Construction of a microcavity-based microfluidic chip with simultaneous SERS quantification of dual biomarkers for early diagnosis of Alzheimer's disease.

Since there is no effective Alzheimer's disease (AD)-modifying therapy available currently, early analysis of AD core biomarkers has become one of great significance and common concern in clinical diagnosis. Herein, we designed an Au-plasmonic shell attached polystyrene (PS) microsphere in a microfluidic chip for simultaneous detection of Aß1-42 and p-Tau181 protein. The corresponding Raman reporters were identified in femto gram level by ultrasensitive surface enhanced Raman spectroscopy (SERS). Both of Raman experimental data and finite-difference time-domain modeling demonstrates the synergetic coupling between PS microcavity with the optical confinement property and the localized surface plasmon resonance (LSPR) of AuNPs, so leading to highly amplified electromagnetic fields at the 'hot spot'. Moreover, the microfluidic system is designed with multiplex testing and control channels in which the AD-related dual proteins were detected quantitatively with a lower limit of 100 fg mL-1. Thus, the proposed microcavity-based SERS strategy initiates a new way for accurately prediction of AD in human blood samples and provides the potential application for synchronous determination of multiple analytes in general disease assays.

Use of sponge iron dosing in baffled subsurface-flow constructed wetlands for treatment of wastewater treatment plant effluents during autumn and winter.

Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the effect of baffled subsurface-flow constructed wetland (BSFCW) with SI dosing to purify the WWTP effluents after the stable operation. The water flow of this BSFCW is the repeated combination of upward flow and downward flow, which can provide a longer treatment pathway and microbial exposure time. The average removal rates of total inorganic nitrogen (TIN) were 27.80%, 30.17%, and 44.83%, and the average removal rates of chemical oxygen demand (COD) were 19.96%, 23.73%, and 18.38%. The average removal rates of total phosphorus (TP) were 85.94%, 82.14%, and 83.95%. Cu/SI improved the dissolution of iron, C/SI improved denitrification, and a winter indoor temperature retention measure was adopted to increase the effectiveness of wetland treatment during the winter months. After comprehensively analyzing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and two-dimensional numerical simulation diagrams, a plausible conjecture that microbes use electrons from SI for autotrophic denitrification is presented. Moreover, the stress effect of wetlands dosed with SI on plants decreased stepwise along the course since C/SI used on wetlands had less impact on plant stress.

1. Treatment of sequencing batch biofilm reactor reaction effluent indoors in low temperature using a combination of three wetland fillers, sponge iron + gravel, copper sulfate modified sponge iron + gravel, and sponge iron coupled carbon source + gravel.2. The use of the baffled subsurface-flow constructed wetland, combined with the distribution simulation diagram, especially the distribution simulation diagram of iron, gives an explanation to the degradation mechanism of the pollutants and the transformation of iron into wetlands.3. An conjecture of electron transport during microbial autotrophic denitrification involving iron is presented, plausibly explaining the variation in treatment effects.

Use of sponge iron as an indirect electron donor to provide ferrous iron for nitrate-dependent ferrous oxidation processes: Denitrification performance and mechanism.

Sponge iron (SI) can serve as an indirect electron donor to provide Fe(II) for the nitrate-dependent ferrous oxidation (NDFO) process, producing OH- and magnetite. The SI-NDFO system mainly uses Fe(OH)2 as an electron donor, achieving a TN reduction rate of 0.42 mg-TN/(gVSS·h) for a period of at least 90 days. The enrichment of iron-oxidizing bacteria and the competition of iron-carbon micro-electrolysis for reaction sites on the surface of SI are the main reasons for the improvement of total nitrogen removal efficiency (TNRE). With an influent NO3--N concentration of 50 mg/L and a SI concentration of 50 g/L (at pH 5.0 and 30 °C), the TNRE reached a maximum level of 38.28%. In addition, reducing the pH environment was found to improve the denitrification efficiency of the SI-NDFO system, although denitrification stability was also reduced as a result. Overall, the SI-mediated NDFO process is a promising technique.