OsOFP6 Overexpression Alters Plant Architecture, Grain Shape, and Seed Fertility.

OVATE family proteins (OFPs) play important roles in plant growth and development, hormone signaling, and stress response pathways. However, the functions of OsOFPs in rice are largely unknown. In this study, a novel gain-of-function rice mutant, Osofp6-D, was identified. This mutant exhibited decreased plant height, erect leaves, reduced panicle size, short and wide seeds, delayed seed germination time, and reduced fertility. These phenotypic changes were attributed to the increased expression of OsOFP6, which was caused by a T-DNA insertion. Complementation of the Osofp6-D phenotype by knockout of OsOFP6 using the CRISPR/Cas9 system confirmed that the Osofp6-D phenotype was caused by OsOFP6 overexpression. In addition, transgenic plants overexpressing OsOFP6 with the 35S promoter mimicked the Osofp6-D phenotype. Cytological observations of the glumes showed that OsOFP6 overexpression altered the grain shape, mainly by altering the cell shape. Hormone response experiments showed that OsOFP6 was involved in the gibberellin (GA) and brassinolide (BR) signaling responses. Further studies revealed that OsOFP6 interacts with E3BB, which is orthologous to the Arabidopsis central organ size-control protein BIG BROTHER (BB). This study further elucidates the regulation mechanism of the rice OFP family on plant architecture and grain shape.

A Survey on Reinforcement Learning Methods in Bionic Underwater Robots.

Bionic robots possess inherent advantages for underwater operations, and research on motion control and intelligent decision making has expanded their application scope. In recent years, the application of reinforcement learning algorithms in the field of bionic underwater robots has gained considerable attention, and continues to grow. In this paper, we present a comprehensive survey of the accomplishments of reinforcement learning algorithms in the field of bionic underwater robots. Firstly, we classify existing reinforcement learning methods and introduce control tasks and decision making tasks based on the composition of bionic underwater robots. We further discuss the advantages and challenges of reinforcement learning for bionic robots in underwater environments. Secondly, we review the establishment of existing reinforcement learning algorithms for bionic underwater robots from different task perspectives. Thirdly, we explore the existing training and deployment solutions of reinforcement learning algorithms for bionic underwater robots, focusing on the challenges posed by complex underwater environments and underactuated bionic robots. Finally, the limitations and future development directions of reinforcement learning in the field of bionic underwater robots are discussed. This survey provides a foundation for exploring reinforcement learning control and decision making methods for bionic underwater robots, and provides insights for future research.

Biomechanical comparison of the effect of bilateral sagittal split ramus osteotomy with or without Le Fort I osteotomy on the temporomandibular joints of the patients with maxillofacial deformities under centric occlusion.

Mandibular deformities negatively affect the daily activities of the patients and may cause temporomandibular disorders (TMD). Bilateral sagittal split ramus osteotomy (BSSRO) and Le Fort I osteotomy are effective treatments to correct the mandibular deformities. The aim of this study was to investigate and compare the effects of the BSSRO with or without Le Fort I on the stress distributions of the temporomandibular joints (TMJs) of the patients with mandibular deformities under centric occlusion based on finite element (FE) method. Preoperative and postoperative cone-beam computed tomography (CBCT) images of twenty-four patients diagnosed with mandibular prognathism, including ten patients with BSSRO and another 14 patients with bimaxillary osteotomy (BSSRO with Le Fort I), were used to construct maxillofacial models. Ten asymptomatic individuals were also performed CBCT scanning and defined as the control group. In addition, the muscle forces and boundary conditions corresponding to centric occlusions were applied on each model. For the preoperative groups with both the BSSRO and bimaxillary osteotomies, the average peak contact stresses of the TMJs were both greater than those of the control group. After the surgeries, the contact stresses of the discs and temporal bones of both groups considerably decreased. However, the contact stresses on the condyles slightly increased after BSSRO but decreased after bimaxillary osteotomy. The TMJs of the patients with maxillofacial deformities suffered abnormal tensile and compressive stresses compared with the asymptomatic subjects under centric occlusion. Both of the BSSRO and bimaxillary osteotomy could improve the risk stress distributions of the TMJs.

Biomechanical analysis of temporomandibular joints during mandibular protrusion and retraction motions: A 3d finite element simulation.

BACKGROUND AND OBJECTIVE: Temporomandibular disorders (TMDs) represent a wide range of musculoskeletal disorders associated with the maxillofacial system, which negatively affect the daily activities of patients. TMD symptoms are caused by the temporomandibular joint (TMJ) overloading. TMJ motions are frequent and can trigger overloading and imbalanced loads on the TMJs, which are assumed to be dangerous. The condyles move forward a lot during mandibular protrusion, which is possibly harmful to the biomechanical environment of the TMJs. The aim of this study was to investigate the biomechanical behavior of TMJs during mandibular protrusion and retraction. METHODS: Six three-dimensional maxillofacial system models from asymptomatic subjects were established through computed tomography (CT) and magnetic resonance imaging (MRI). The mandibular protrusion and retraction were recorded using an optical tracking system. Finite element analysis was used to simulate the biomechanical behaviors of the TMJs during the movements. RESULTS: The simulation results were validated to be effective by comparison with the MRIs. The results indicated that the stresses during the protrusion and retraction were approximately equal at the same condylar displacement. Meanwhile the discal stresses, relatively correlated with the condylar displacement, increased as the condylar displacement increased during the protrusion and decreased as the condylar displacement decreased in the retraction. In addition, the average peak maximum and minimum principal stresses of the discs were 0.186 and -0.192 MPa, respectively. CONCLUSIONS: The models were reasonable for the investigation of the TMJs motion. Based on the results, three quadratic polynomials were proposed to describe the relationship between the stresses and the condylar displacements. In clinical diagnosis, the functions are helpful in the prediction of the discal stresses by measuring the condylar displacement.

Photonic Crystal Polymeric Thin-Film Dye-Lasers for Attachable Strain Sensors.

In this report, using two-dimensional photonic crystals (PhC) and a one-dimensional PhC nano-beam cavity, we realized the development of all-polymeric dye-lasers on a dye-doped, suspended poly-methylmethacrylate film with a wavelength-scale thickness. In addition to the characterization of basic lasing properties, we also evaluated its capacity to serve as an attachable strain sensor. Through experimentation, we confirmed the stable lasing performances of the dye-laser attaching on a rough surface. Moreover, we also theoretically studied the wavelength responses of the utilized PhC resonators to stretching strain and further improved them via the concept of strain shaping. The attachability and high strain sensing response of the presented thin film PhC dye-lasers demonstrate their potential as attachable strain sensors.

Temporomandibular condylar articulation and finite helical axis determination using a motion tracking system.

Kinematics play an important role in assessing the recovery of the patients' temporomandibular joint (TMJ) and occlusal functions. The finite helical axis (FHA), which simplifies three parameters in Euler-angle descriptions, provides a comprehensive insight into TMJ kinematics. Additionally, the FHA is one of the potential indicators used in the diagnosis and treatment of TMDs and the design and use of the TMJ replacement. This study aimed to illustrate the changes in the FHA of the TMJs during basic mandibular motions. Visible markers were rigidly affixed to the mandibular dentition and a helmet. Four active motions were registered: mouth opening, mandibular protrusion, and left and right lateral protrusions. According to the models reconstructed from the computed tomography of the same subject and the relative distance of the markers, subject-specific condylar tracking was achieved, and the FHAs for the four motions were determined. In addition to the irregular distribution in the initial opening, the FHA of the opening formed an "L-shaped" curve. Mandibular protrusion is a translational motion with little rotation. Additionally, the FHA crossed the ipsilateral TMJ during lateral protrusions, from initially vertical directions generally to horizontal directions at the front view. The proposed method provides a feasible way for measuring the FHA.