Effect of breathing exercises on improving oral feeding ability of premature infants / 中国实用护理杂志

Objective To investigate the effects of respiratory training on oral feeding ability, feeding response, transition time, hospitalization time, gastric tube indwelling time and recovery time to birth weight in premature infants. Methods The Neonatal Intensive Care Unit(NICU) preterm infants who were in accordance with the inclusion criteria were divided into the observation group and the control group according to the random number method. The observation group was 20 cases, the control group was 21 cases. The control group implemented NICU routine nursing, including oral nursing, umbilical nursing, body position nursing. The observation group received respiratory training exercises on the basis of the control group, and the oral feeding ability of the two groups of premature infants was evaluated and recorded after intervention at the beginning of 0, 5, 10, 15 d.The feeding reaction, transition time, the hospitalization time and gastric tube indwelling time were recorded in two groups of preterm infants. Results The non-nutritive sucking scores after intervention at the beginning of 0, 5, 10, 15 d were (31.10±5.57), (37.50±4.86), (53.20±7.60), (74.90±4.77) points in the observation group . The non-nutritive sucking scores after intervention at the beginning of 0, 5, 10, 15 d were (31.91±5.56), (33.36±6.17), (45.54±5.39), (62.82±6.78) points in the control group. There was no significant difference after intervention at the beginning of 0, 5 d between the two groups (t=-0.333,1.694, all P>0.05), but had the significant difference after intervention at the beginning of 10 ,15 d (t=2.683, 4.676, all P <0.05). The transition time,gastric tube indwelling time and recovery time to birth weight in premature infants was (7.15±1.72), (11.00±2.37), (7.86±1.43) d in the observation group and (10.33±2.44), (16.14±2.29), (9.97±1.69) d in the control group, and there were significant differences between the two groups(t=-4.807, 3.335,-9.905, P <0.05 ). There was no significant difference in the hospitalization time between the two groups(P>0.05). Conclusions Breathing exercises can improve the oral feeding ability and improve the feeding effect of premature infants.

Determination of Chloride in Infant Formula and Adult/Pediatric Nutritional Formula by Potentiometric Titration: Single-Laboratory Validation, First Action 2015.07.

A potentiometric method for determination of chloride was validated against AOAC Standard Method Performance Requirement (SMPR(®)) 2014.015. Ten AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) matrixes, including National Institute of Standards and Technology (NIST) Standard Reference Material (SRM) 1849a, were tested in duplicate on 6 independent days. The repeatability (RSDr) ranged from 0.43 to 1.34%, and the intermediate reproducibility (RSDiR) ranged from 0.80 to 3.04%. All results for NIST SRM 1849a were within the range of the certified concentration (701 ± 17 mg/100 g). Recovery was demonstrated with two overspike levels, 50 and 100%, in the 10 SPIFAN matrixes. Samples were tested in duplicate on 3 different days, and all results were within the SMPR requirement of 95 to 105%. The LOQs of the method for powdered products and ready-to-feed or reconstituted products were 20 mg/100 g and 2.2 mg/100 mL, respectively. A wide analytical range from the LOQ to 99.5% chlorine content can be reached with an appropriate dilution factor, but in practice, the upper analytical value observed in routine matrix testing was approximately 1080 mg/100 g in skim milk powder. This is a rapid, simple, and reliable chlorine-testing method applicable to infant formula, adult nutritionals, and ingredients used in these dairy-based products, such as skim milk powder, desalted whey powder, whey protein powder, and whole milk powder.

Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin.

The goal of this study was to provide practical strategies for purifying genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin (BCG-DNA) by ion-exchange chromatography. A multistep process was developed to purify BCG-DNA. The process consisted of sonication, heating, trypsin digestion, ion-exchange chromatography, gel-filter chromatography, and lyophilization. After ion-exchange chromatography, BCG-DNA was highly purified and possessed potent biological effects. The methods described were efficient and had good reproducibility. Further, this was the first reported chromatography method to purify BCG-DNA.

THE PREVENTIVE AND THERAPEUTIC EFFECTS OF SILK FIBROIN ACTIVE PEPTIDES ON ACUTE ALCOHOLISM IN MICE / 营养学报

Objective:To study the preventive and therapeutic effects of silk fibroin peptides (SFP) on acute alcoholism in mice. Methods:In first experiment, the mice were randomly divided into four groups, and every group was treated by 56o alcohol (ethanol dosage 7.56 ml/kg bw) via i.g. Eighty mice were fed normal saline (NS) and different dosages of SFP (0.1, 0.5, 2.5 g/kg bw) 30 min later respectively, then the rate of ebriety and time of sobriety were determined. Another 72 mice were also divided into four groups and given NS and SFP similarly. The concentration of ethanol in serum was measured 1 h, 2 h and 4 h later respectively. In second experiment, the mice were also divided into four similar groups, but 56o alcohol was given at 6.16 ml/kg?bw via i.g. NS and SFP were given similarly, 0.5 h before alcohol. Two experiments were performed to observe the effect of SFP on prevention of temulence. Results:The time of sobriety and concentration of ethanol of SFP groups fed 0.5 and 2.5g/kg bw were lower significantly than those of NS group (P

Screening out CpG immunostimulatory sequence-specific DNA-binding proteins with Yeast-one-hybrid system / 第二军医大学学报

Objective: To screen out DNA binding proteins specifically recognizing CpG immunostimulatory sequence (ISS) for further investigating the molecular mechanisms of ISS. Methods: Yeast one hybrid system was adapted in screening a human bone marrow cDNA library using 4 copies of ISS as bait. The ISS binding activity of the positive clone was confirmed by electrophoretic mobility shift assay (EMSA). Results: Four dual positive colonies were obtained, two of them encoded proteins with unknown functions. The other 2 encoded light chains of immunoglobulin with amino sequences homology to anti DNA Ab and HBsAb respectively. EMSA showed HBsAb specifically bound to CpG ODN at pH6.4 and pH 5.8. Conclusion: HBsAb may have ISS specific DNA binding activity.