Association of MTR and MTRR genes and oral health-related quality of life in children with dental caries.

This study aimed to assess whether genetic polymorphisms in MTR and MTRR are potential biomarkers of oral health-related quality of life (OHRQoL) in children with caries. A cross-sectional study was designed wherein pairs of parents/caregivers and children (aged two-five years) were selected. Clinical examination was used to detect dental caries, which were classified as low-severity and high-severity caries. The Early Childhood Oral Health Impact Scale (ECOHIS) questionnaire was used to assess OHRQoL. Genomic DNA extracted from the saliva was used to analyze two missense genetic polymorphisms: MTR (rs1805087) and MTRR (rs1801394). Mann-Whitney non-parametric test was used to analyze candidate genes with OHRQoL scale and domain, with a significance level of p≤0.05. MTR (rs1805087) was found associated (p = 0.05) with children's OHRQoL subscale scores in the dominant model (GG + AG). Genetic polymorphisms in MTR may increase the risk of poor OHRQoL in children with caries. Further studies are needed to investigate genetics, molecular factors, and OHRQoL.

Association of MTR and MTRR genes and oral health-related quality of life in children with dental caries

Abstract This study aimed to assess whether genetic polymorphisms in MTR and MTRR are potential biomarkers of oral health-related quality of life (OHRQoL) in children with caries. A cross-sectional study was designed wherein pairs of parents/caregivers and children (aged two-five years) were selected. Clinical examination was used to detect dental caries, which were classified as low-severity and high-severity caries. The Early Childhood Oral Health Impact Scale (ECOHIS) questionnaire was used to assess OHRQoL. Genomic DNA extracted from the saliva was used to analyze two missense genetic polymorphisms: MTR (rs1805087) and MTRR (rs1801394). Mann-Whitney non-parametric test was used to analyze candidate genes with OHRQoL scale and domain, with a significance level of p≤0.05. MTR (rs1805087) was found associated (p = 0.05) with children's OHRQoL subscale scores in the dominant model (GG + AG). Genetic polymorphisms in MTR may increase the risk of poor OHRQoL in children with caries. Further studies are needed to investigate genetics, molecular factors, and OHRQoL.

Do Antimicrobial Photodynamic Therapy and Low-Level Laser Therapy Minimize Postoperative Pain and Edema After Molar Extraction?

PURPOSE: This study was designed to examine the efficacy of antimicrobial photodynamic therapy (aPDT) and low-level laser therapy (LLLT) in reducing postoperative pain and edema after molar extractions. METHODS: The investigators designed a single-blind randomized controlled study and enrolled subjects requiring extraction of at least one of the first or second molars. They were randomized to one of the following four groups: control group, aPDT group, LLLT group, and aPDT and LLLT group. Patients were blinded to the group assigned. The outcome variables were postoperative pain and edema. Pain intensity was measured on a visual analog scale (recorded every day for 7 days after tooth extraction). Facial edema was assessed by measuring the perimeter between the tragus, base of the jaw, and labial commissure, which was recorded once before surgery and then on the third and seventh days after surgery. Other variables were age, sex, ethnicity, decayed/missing/filled teeth, and tooth types. Appropriate univariate and bivariate statistics were computed and statistical significance was set at a value of P < .05. RESULTS: The sample was composed of 40 patients with a mean age of 41.25 ± 13.97 years and 25 (62.5%) of them were women. There were 10 subjects in each treatment group. The mean of postoperative pain within the groups was associated with a significant continuous decrease over time (P < .05). Postoperative pain was lowest in the aPDT + LLLT group in the 1st, 2nd, 3rd, 5th, 6th, and 7th day after tooth extraction (P < .05). There were no significant differences in edema among the groups (P > .05). CONCLUSION: The combined use of aPDT and LLLT was effective in reducing postoperative pain. These procedures can be applied in everyday surgical practice.

Determination of TNF-a Gene Polymorphisms on Skeletal Pattern in Class II Malocclusion.

Bone development and growth is a non-going, life-long process, varying greatly among individuals and much of this variation could be modulated by genetic factors. The purpose of this study was to evaluate the association between the polymorphisms in the TNF-a gene and skeletal class II malocclusion. Single nucleotide polymorphisms in TNF-a (rs1799724; rs1800629) gene were studied in 79 skeletal class II malocclusion and 102 skeletal class I malocclusion subjects from Straight Wire Group of Studies on Orthodontics and Functional Orthopedics for Maxillary from Rio de Janeiro, Brazil. The Genotyping of these selected polymorphisms was carried out by TaqMan real-time PCR using genomic DNA extracted from buccal cells. All allele and genotype frequencies were compared between the groups using the PLINK® software in a free, in a dominant and in a recessive model using a chi-square test (p≤0.05). There was no significant association of TNF-a (rs1799724; rs1800629) genotype and allele distribution with skeletal class II malocclusion. Regardless of the dominant or recessive genetic model, the preferential genotype associations for rs1799724 and rs1800629 was insignificant. In conclusion, no evidence of association is apparent between genetic polymorphisms involving TNF-a and skeletal class II malocclusion or the position of the maxilla and mandible in the postero-anterior direction.

Determination of tnf-a gene polymorphisms on skeletal pattern in class ii malocclusion

Abstract Bone development and growth is a non-going, life-long process, varying greatly among individuals and much of this variation could be modulated by genetic factors. The purpose of this study was to evaluate the association between the polymorphisms in the TNF-a gene and skeletal class II malocclusion. Single nucleotide polymorphisms in TNF-a (rs1799724; rs1800629) gene were studied in 79 skeletal class II malocclusion and 102 skeletal class I malocclusion subjects from Straight Wire Group of Studies on Orthodontics and Functional Orthopedics for Maxillary from Rio de Janeiro, Brazil. The Genotyping of these selected polymorphisms was carried out by TaqMan real-time PCR using genomic DNA extracted from buccal cells. All allele and genotype frequencies were compared between the groups using the PLINK® software in a free, in a dominant and in a recessive model using a chi-square test (p≤0.05). There was no significant association of TNF-a (rs1799724; rs1800629) genotype and allele distribution with skeletal class II malocclusion. Regardless of the dominant or recessive genetic model, the preferential genotype associations for rs1799724 and rs1800629 was insignificant. In conclusion, no evidence of association is apparent between genetic polymorphisms involving TNF-a and skeletal class II malocclusion or the position of the maxilla and mandible in the postero-anterior direction.

Resumo O desenvolvimento e crescimento ósseo é um processo contínuo, que dura toda a vida, variando muito entre os indivíduos e grande parte dessa variação pode ser modulada por fatores genéticos. O objetivo deste estudo foi avaliar a associação entre os polimorfismos no gene TNF-a e a má oclusão da classe II esquelética. Polimorfismos no gene TNF-a (rs1799724; rs1800629) foram estudados em 79 indivíduos com má oclusão esquelética de classe II e 102 indivíduos com má oclusão esquelética classe I do Grupo de Estudos em Ortodontia e Ortopedia Funcional dos Maxilares do Rio de Janeiro, Brasil. A genotipagem destes polimorfismos foi realizada por PCR em tempo real, através de DNA genômico extraído de células bucais. Todas as frequências alélicas e genotípicas foram comparadas entre os grupos utilizando o software PLINK® em um modelo livre, dominante e recessivo. Foi aplicado o teste do qui-quadrado (p≤0,05). Não houve associação significativa na distribuição genotipica e alélica do gene TNF-a (rs1799724; rs1800629) com a má oclusão de classe II esquelética. Independentemente do modelo genético dominante ou recessivo, as associações genotípicas preferenciais para rs1799724 e rs1800629 foram insignificantes. Pode-se concluir que, não existe evidência de associação entre polimorfismos genéticos envolvendo TNF-a e má oclusão esquelética de classe II ou a posição da maxila e mandíbula na direção póstero-anterior.

Avaliação comparativa da alteração dimensional de diferentes materiais utilizados para a confecção de troquéis / Comparative evaluation of dimensional change of different materials used to die making

Neste trabalho experimental, objetivou-se avaliar comparativamente a alteração dimensional de três diferentes materiais utilizados na confecção de troqueis: gesso tipo IV – Fuji Rock (GC), resina epóxica industrial – Sikadur 32 (Sika) e resina epóxica – Tri-Epoxy Die Material (Tri-Dynamics). Foram confeccionados dois modelos-padrão em aço inoxidável. Um desses modelos foi confeccionado de modo a simular um preparo para coroa total em um pré-molar. O outro modelo foi confeccionado 2,0mm maior em todas as dimensões em relação ao primeiro de modo a proporcionar um alívio uniforme para a base leve do material de moldagem. Trinta troquéis foram obtidos a partir de moldes em silicona de adição (Aquasil – Dentsply) utilizada com a técnica de dois passos. Os troqueis foram divididos em grupos de dez para cada material. As alterações foram mensuradas através das medições da altura e dos diâmetros da base e do topo dos troqueis obtidos, com o auxílio de um Projetor de Perfis Deltronic DV-114 com leitura em software. As medidas obtidas foram dispostas em tabelas e analisadas estatisticamente. Após avaliação pode-se concluir que houve diferença estatisticamente significante entre todos os grupos testados. Os troquéis de gesso tipo IV Fuji Rock (GC) contraíram nas medições da altura e do diâmetro do topo, e expandiram nas medições do diâmetro da base, apresentando diferença estatisticamente significativa em todas as comparações com o grupo controle (metal). Os troquéis de resina epóxica Tri-Epoxy (Tri-Dynamics) contraíram nas medições da altura e do diâmetro do topo, e expandiram nas medições diâmetro da base, apresentando diferença estatisticamente significante somente no diâmetro do topo nas comparações com o grupo controle (metal). Os troquéis de resina epóxica Sikadur 32 (Sika) contraíram nas medições da altura e do diâmetro do topo, e expandiram nas medições do diâmetro das base, apresentando diferença estatisticamente significante ...

The aim of this study was to comparatively evaluate the dimensional changes of three different materials used in the production of dies: gypsum type IV - Fuji Rock (CG), industrial epoxy resin - Sikadur 32 (Sika) and epoxy resin - Tri-Epoxy Die Material (Tri-Dynamics). Two standard models of stainless steel have been made. One of these models was prepared to simulate a full crown preparation on a premolar. The other model was prepared 2.0 mm larger in all dimensions compared to the first one so as to provide an uniform relief for impression material mild base. Thirty dies have been made from molds in vinyl polysiloxane (Aquasil - Dentsply) using the two steps technique. Dies were divided into groups of ten for each material. Changes were evaluated by measuring the height and diameter of bottom and top of the dies with the aid of a Deltronic DV-114 Profiles Projector with reading software. The resultant measurements were arranged into tables and statistically analyzed. After evaluation, it was concluded that there was a statistically significant difference among all tested groups. Gypsum Fuji Rock type IV (GC) dies shrank in top’s height and diameter measurements, and expanded the bottom diameter measurements, showing statistically significant differences when compared to control group (metal). Epoxy resin Epoxy-Tri (Tri-Dynamics) dies shrank in top’s height and diameter and expanded in bottom diameter measurements, showing statistically significant difference only in top’s diameter when compared to control group (metal). Epoxy resin Sikadur 32 (Sika) dies shrank in top’s height and diameter measurements and expanded in bottom’s diameter measurements, showing a statistically significant difference when comparing to control group (metal).