Polyphenolic Profile and Antioxidant Activity of Whole Grape Juices from Vitis labrusca and Brazilian Hybrid Grapes in Two Training Systems.

The phenolic profile and antioxidant activity of whole grape juices from Vitis labrusca and Brazilian hybrids in two training systems were analyzed. Genotypes of V. labrusca ('Bordô' and 'Isabel') and Brazilian hybrids ('IAC 138-22 Máximo' and 'BRS Violeta') were grafted onto the rootstock 'IAC 766 Campinas' (106-8 'Mgt' × Vitis caribaea) and trained on low and high trellis. After harvest, the grapes were destemmed and the berries macerated in a roller crusher. Following hot extraction without pressurization of the pomace and gentle pressing of the blend (skins, must, and seeds), the juices were bottled in amber glass bottles and pasteurized. The physicochemical and colorimetric parameters of the juices, as well as the levels of flavonoids, phenolic compounds, total monomeric anthocyanins, antioxidant activity, and polyphenolic profile, were evaluated. The juices were also subjected to sensory analysis (CAAE: 65549817.7.0000.5411). There was broad variation in all assessed characteristics. The results obtained demonstrate that the training system and grape genotype used in juice production are highly related to the presence of sugars, acidity, and bioactive compounds. Juices made from 'Bordô', 'IAC 138-22 Máximo' and 'BRS Violeta' grapes stood out from 'Isabel' juices, the main grape variety used in Brazilian juice and wine production. All juices contain bioactive compounds in considerable concentrations, indicating beverages with high antioxidant activity and, consequently, high biological potential, with the use of high trellis in vine cultivation potentially increasing concentrations.

Pertinencia de los laboratorios de simulación como herramienta de educación avanzada en salud / Relevance of simulation laboratories as a tool for advanced health education

RESUMEN El constante perfeccionamiento del proceso educativo del hombre demanda el enriquecimiento de las prácticas de la educación avanzada. Es preciso efectuar transformaciones en el trabajo educativo, por lo que resulta indispensable que se oriente de forma tal que permita potenciar en cada estudiante aquellos aspectos que le faciliten conocer, de una manera más significativa, el momento histórico en que viven. Los simuladores virtuales, como método de aprendizaje en ciencias médicas, son potenciadores del aprendizaje y ayudan a evadir el obstáculo de horas limitadas en la práctica clínica, grupos numerosos, inconsistencias en la observación de enfermedades de alto riesgo o inusuales. En Cuba, la utilización de estos no ha sido explotada al máximo. Los estudios que han evaluado la efectividad de estos simuladores en la mejoría del desempeño de los profesionales de la salud, han arrojado resultados muy positivos, pero el elevado precio de los simuladores ha constituido la mayor barrera para su completa introducción en Cuba. Este trabajo expresa la posición de los autores sobre la pertinencia de los laboratorios de simulación como herramienta de educación avanzada en salud. El uso de los laboratorios de simulación en la educación médica, es una práctica positiva en el perfeccionamiento de las competencias profesionales y ofrece la seguridad de la atención sanitaria; no es un sustituto de la práctica supervisada en entorno real, sino un complemento deseable para una pericia segura y efectiva que permite desarrollar habilidades, conocimientos y actitudes, es decir, capacidades para su óptimo desempeño.

ABSTRACT The constant improvement of the educational process of man demands the enrichment of the practices of advanced education. It is necessary to carry out transformations in the educational work, so it is essential that it be oriented in such a way that it allows each student to enhance those aspects that make it easier for them to know, in a more significant way, the historical moment in which they live. Virtual simulators, as a learning method in medical sciences, are learning enhancers and help to avoid the obstacle of limited hours in clinical practice, large groups, inconsistencies in the observation of high-risk or unusual diseases. In Cuba, the use of these has not been exploited to the maximum. The studies that have evaluated the effectiveness of these simulators in improving the performance of health professionals have yielded very positive results, but the high price of the simulators has constituted the greatest barrier to their full introduction in Cuba. This work expresses the position of the authors on the relevance of simulation laboratories as a tool for advanced health education. The use of simulation laboratories in medical education is a positive practice in the improvement of professional skills and offers the security of health care, it is not a substitute for supervised practice in a real environment, but a desirable complement to an expertise safe and effective, which allows developing skills, knowledge and attitudes, that is, capacities for optimal performance.

Association of body mass index with Juvenile Idiopathic Arthritis disease activity: a Portuguese and Brazilian collaborative analysis.

OBJECTIVE: To investigate the relationship between body mass index (BMI) and disease activity in patients with Juvenile Idiopathic Arthritis (JIA). METHODS: Patients with JIA, aged ≤18 years, registered at the Rheumatic Diseases Portuguese Register (Reuma.pt) in Portugal and Brazil were included. Age- and sex-specific BMI percentiles were calculated based on WHO growth standard charts and categorized into underweight (P <3), normal weight (3≤P≤85), overweight (85

97). Disease activity was assessed by Juvenile Arthritis Disease Activity Score (JADAS-27). Uni- and multivariate analyses were performed. RESULTS: A total of 275 patients were included. The prevalence of underweight, normal weight, overweight and obesity was 6.9%, 67.3%, 15.3% and 10.5%, respectively. Underweight patients had significantly higher number of active joints (p <0.001), patient's/parent's global assessment of disease activity (PGA) (p=0.020), physician's global assessment of disease activity (PhGA) (p <0.001), erythrocyte sedimentation rate (ESR) (p=0.032) and overall higher JADAS-27 (p <0.001), compared to patients with normal weight, overweight and obesity. In the multivariate regression, underweight persisted significantly associated with higher disease activity, compared to normal weight (B=-9.430, p <0.001), overweight (B=-9.295, p=0.001) and obesity (B=-9.120, p=0.001), when adjusted for age, gender, country, ethnicity, JIA category and therapies used. The diagnosis of RF- (B=3.653, p=0.006) or RF+ polyarticular JIA (B=5.287, p=0.024), the absence of DMARD therapy (B=5.542, p <0.001) and the use of oral GC (B=4.984, p=0.002) were also associated with higher JADAS-27. CONCLUSION: We found an independent association between underweight and higher disease activity in patients with JIA. Further studies are needed to understand the underlying mechanisms of this association.

Ele não chega nunca... experiências de mulheres no contexto do trabalho escravo / He never comes back... experiences of women in the slave labor context

O artigo discute situações de medo, resistência e estranhamento vividos em consequência de migrações, a partir do discurso de três camponesas do Piauí. Elas têm em comum a região de moradia, o trabalho rural, a baixa escolaridade, a situação de pobreza, a “invisibilidade” de gênero, e, sobretudo, a existência de familiares masculinos aliciados e levados ao sul do Estado do Pará: muitos desses homens, frequentemente submetidos ao que tem sido reconhecido como trabalho escravo por dívida em empresas agropecuárias, passam por situações de violência e as suas famílias não têm notícias sobre eles. Nas comunidades de origem, esposas, mães, filhas, mantêm a vida cotidiana, compartilhando redes de relações materiais e simbólicas.(AU)

This article discusses situations of fear, resistance and estrangement due to migration, based on the discourse of three peasant women in Piauí, Northeast Brazil. These women have much in common: housing conditions, rural work, poor education, poverty, “invisibility” of gender, and male relatives who were deceived and taken to south Pará, North Brazil. Many of these men, often subjected to what has been recognized as debt bondage in agricultural companies, are submitted to violence situations and their families have no news of them. In their origin communities, wives, mothers and daughters lead their daily life by sharing networks of material and symbolic relations.(AU)

Effect of different hormone protocols on the pregnancy rate of Nelore cows inseminated at fixed time / Efeito de diferentes protocolos hormonais sobre a taxa de prenhez de vacas Nelore inseminadas em tempo fixo

The aim of this study was to evaluate the effect of different hormonal treatments on Nelore cows pregnant rates inseminated at fixed time. Sixty nine cows were used, distributed in treatments T1 (n=15) 50µg Fertirelin day 0 (D0), 530µg PGF2 D7, 50µg Fertirelin D9; T2 (n=14) 25µg Fertirelin D0, 265µg PGF2 D7, 25µg Fertirelin D9; T3 (n=15) 530µg PGFD0, 530µg PGFD11, 50µg Fertirelin D13; T4 (n=12) 265µg PGFD0, 265µg PGFD11, 25µg Fertirelin D13; T5 (n=13) 265µg PGFD0, 265µg PGF2 D11, 50µg Fertirelin D13. The biggest follicle diameter was ultrasonographically measured before the last Fertirelin administration in T1 (0.98±0.3); T2 (1.20±0.3); T3 (1.22±0.2); T4 (1.02±0.4) and T5 (1.09±0.3), not showing statistical difference (P>0.05). The pregnancy diagnosis was accomplished on days 45 and 60 post insemination, with pregnancy rates of 20% (3/15); 50% (7/14); 40% (6/15); 50% (6/ 12); 30.8% (4/13) for T1, T2, T3, T4 e T5, respectively, not showing statistical difference as well (P>0.05). It was concluded that the reduction of hormonal doses used, despite the protocol, do not interfere on pregnancy rates at first service in cyclical Nelore cows.

Objetivou-se com esse trabalho avaliar o efeito de diferentes protocolos hormonais sobre a taxa de prenhez de vacas Nelore inseminadas artificialmente em tempo fixo (IATF). Foram utilizadas 69 vacas, distribuídas nos tratamentos T1 (n=15) 50µg Fertirelina dia 0 (D0), 530µg PGF2 D7, 50µg Fertirelina D9; T2 (n=14) 25µg Fertirelina D0, 265µg PGFD7, 25µg Fertirelina D9; T3 (n=15) 530µg PGFD0, 530µg PGF D11, 50µg Fertirelina D13; T4 (n=12) 265µg PGFD0, 265µg PGFD11, 25µg Fertirelina D13; T5 (n=13) 265µg PGFD0, 265µg PGFD11, 50µg Fertirelina D13. Antes da última aplicação de Fertirelina, foi mensurado ultra-sonograficamente o diâmetro (cm) do maior folículo em T1 (0,98±0,3); T2 (1,20±0,3); T3 (1,22±0,2); T4 (1,02±0,4) e T5 (1,09±0,3), não diferindo estatisticamente (P>0,05). O diagnóstico de gestação foi rea¬lizado nos dias 45 e 60 pós-inseminação, com taxas de prenhez de 20% (3/15); 50% (7/ 14); 40% (6/15); 50% (6/12); 30,8% (4/13) para T1, T2, T3, T4 e T5, respectivamente, também não apresentando diferença estatística (P>0,05). Concluiu-se que a redução nas doses hormonais utilizadas, independentemente do protocolo, não interferiu nas taxas de prenhez ao primeiro serviço em vacas Nelore cíclicas.

Effect of different hormone protocols on the pregnancy rate of Nelore cows inseminated at fixed time / Efeito de diferentes protocolos hormonais sobre a taxa de prenhez de vacas Nelore inseminadas em tempo fixo

The aim of this study was to evaluate the effect of different hormonal treatments on Nelore cows pregnant rates inseminated at fixed time. Sixty nine cows were used, distributed in treatments T1 (n=15) 50µg Fertirelin day 0 (D0), 530µg PGF2 D7, 50µg Fertirelin D9; T2 (n=14) 25µg Fertirelin D0, 265µg PGF2 D7, 25µg Fertirelin D9; T3 (n=15) 530µg PGFD0, 530µg PGFD11, 50µg Fertirelin D13; T4 (n=12) 265µg PGFD0, 265µg PGFD11, 25µg Fertirelin D13; T5 (n=13) 265µg PGFD0, 265µg PGF2 D11, 50µg Fertirelin D13. The biggest follicle diameter was ultrasonographically measured before the last Fertirelin administration in T1 (0.98±0.3); T2 (1.20±0.3); T3 (1.22±0.2); T4 (1.02±0.4) and T5 (1.09±0.3), not showing statistical difference (P>0.05). The pregnancy diagnosis was accomplished on days 45 and 60 post insemination, with pregnancy rates of 20% (3/15); 50% (7/14); 40% (6/15); 50% (6/ 12); 30.8% (4/13) for T1, T2, T3, T4 e T5, respectively, not showing statistical difference as well (P>0.05). It was concluded that the reduction of hormonal doses used, despite the protocol, do not interfere on pregnancy rates at first service in cyclical Nelore cows.

Objetivou-se com esse trabalho avaliar o efeito de diferentes protocolos hormonais sobre a taxa de prenhez de vacas Nelore inseminadas artificialmente em tempo fixo (IATF). Foram utilizadas 69 vacas, distribuídas nos tratamentos T1 (n=15) 50µg Fertirelina dia 0 (D0), 530µg PGF2 D7, 50µg Fertirelina D9; T2 (n=14) 25µg Fertirelina D0, 265µg PGFD7, 25µg Fertirelina D9; T3 (n=15) 530µg PGFD0, 530µg PGF D11, 50µg Fertirelina D13; T4 (n=12) 265µg PGFD0, 265µg PGFD11, 25µg Fertirelina D13; T5 (n=13) 265µg PGFD0, 265µg PGFD11, 50µg Fertirelina D13. Antes da última aplicação de Fertirelina, foi mensurado ultra-sonograficamente o diâmetro (cm) do maior folículo em T1 (0,98±0,3); T2 (1,20±0,3); T3 (1,22±0,2); T4 (1,02±0,4) e T5 (1,09±0,3), não diferindo estatisticamente (P>0,05). O diagnóstico de gestação foi rea¬lizado nos dias 45 e 60 pós-inseminação, com taxas de prenhez de 20% (3/15); 50% (7/ 14); 40% (6/15); 50% (6/12); 30,8% (4/13) para T1, T2, T3, T4 e T5, respectivamente, também não apresentando diferença estatística (P>0,05). Concluiu-se que a redução nas doses hormonais utilizadas, independentemente do protocolo, não interferiu nas taxas de prenhez ao primeiro serviço em vacas Nelore cíclicas.