[Changes in lipid content of RBC membranes in divers working on low and moderate depths.]

The study covered lipid content of RBC membranes in divers exposed to extreme hyperbaric factors at work. Findings are that prophylactic intake of biologically active additive nutrient "Kalifen" made of viburnum berries preserved physiologic characteristics of RBC and recovery of lipid components in their membranes.

[Hepatorotective activity of honeysuckle fruit extract in carbon tetrachloride intoxicated rats].

We have studied the effects of the extract from the dry pomace of honeysuckle fruits Lonicera edulis Turcz. and commercial reference preparation Legalon on weight and biochemical liver indexes in rats upon carbon tetrachloride intoxication. The extract of honeysuckle fruits favored a decrease in the total lipid content, reduced specific liver weight, and facilitated restoration of the activity of serum alanine aminotransferase and liver lysosomal enzymes. It the blood of rats, the extract increased superoxide dismutase activity, decreased glutathione level, enhanced antiradical activity, and reduced the level of malonic dialdehyde. The effect of administration of honeysuckle fruits pomace extract on restoration of the liver function was more pronounced than the effect of Legalon.

[Hepatoprotective properties of isoflavonoids from roots of Maackia amurensis on experimental carbon tetrachloride-induced hepatic damage].

Hepatoprotective properties of ethanol extract from the roots of Maackia amurensis Ruper et Maxim have been studied on the model of toxic hepatitis induced by carbon tetrachloride damage. It is established that the extract contains daidzein, 7-O-gentobiosides of isoflavonoids genistein, formononetin, pseudobabtige-nin, and 5-O-methylgenistein, and 3-O-gentobiosides of pterocarpans (6aR, 11aR)-maakiain and (6aR, 11aR)-medicarpin. The administration of extract facilitates the restoration of antioxidant protection enzymes activity and reduced glutathione level, decreases the formation of toxic peroxidation products, produces normalizing impact on liver phospholipid pattern, and improves the erythrocyte tolerance to hemolytic agents. The action of isoflavonoids from Maackia amurensis in restoration of metabolic pathways of the liver and removal of toxic stress was more effective as compared to that of the reference hepatoprotector legalon.

[Prophylaxis of carbon bisulphide poisoning effects on lipid component of erythrocyte membrane in experiment].

Preventive injection of "Kalifen" extract of high cranberry before and during intoxication with carbon bisulphide appeared to preserve lipid component of RBC membrane in rats.

[Prevention of stress-related disorders in medical personnel].

Study covered female surgeons subjected to occupational stress complex (psychoemotional, chemical, work intensity, etc). Prophylactic intake of biologically active food supplement made of high cranberry appeared to relieve tension of antioxidant and antiradical defence systems, increased activation and stress, to normalize carbohydrates metabolism parameters.

[Influence of the extract from tunic of purple ascidium sea hydrobiont on the lipid metabolism in liver under acute stress conditions].

The effect of an extract from tunic of the sea hydrobiont purple ascidium (Halocynthia aurantium) on the lipid metabolism in the liver of mice has been studied under the acute stress conditions modeled by fixing animals for 22 h at the dorsal neck fold. The extract from purple ascidium tunic is superior to essentiale (well-known commercial hepatoprotector) in the efficacy of restoring lipid fraction content (tryglycerides, cholesterol, free fat acids, lysophospholipids) disturbed by stress action. In addition, the extract normalizes the esterification function of the liver. The observed effect of the purple ascidium tunica extract is related to a combined action of the "marine" phospholipids that are present in the preparation and are known to possess reparative properties.

[Influence of hyperbaric stress on metabolic reactions of the organism of divers, prevention].

Research of metabolic rates is conducted in a blood of the subjects whose professional work is connected with the systematic implementation of diving on the large and medium depths. For restoration of working capacity and preservation of health of divers was carried pharmacological prophylaxis with supplementation "Kalifen", which is the total polyphenol complex isolated from viburnum (Viburnum sargentii Koehne). The blood plasma of divers, investigated after a dive, there was hypertriglyceridemia and hypercholesterolemia, the suppression of esterified liver function, an imbalance of fractional content of phospholipids, the strain of antioxidant defense of an organism and increased lipid peroxidation. Preventive administration supplementation "Kalifen" for 2 months before the dive will help remove metabolic disorders caused by hyperbaric factors, and also to store up of anabolic reserves and increase body resistance level in extreme conditions.

[Application of food supplement, of a duilder-rose for prevention of stressful disturbance of physiological and biochemical characteristics of erythrocytes].

In the article results of researches of influence of food supplement allocated of a guelder-rose (Viburnum sargentii Koehne) on structural and physiological characteristics of erythrocytes are presented to blood of the doctors-surgeons who are exposed during of the working day to complex stressful influence (psychologic-emotional, chemical, intensity of labour process, etc). It is shown that preventive application a polyphenolic complex from a guelder-rose promoted restoration of average volume and diameter of erythrocytes, normalization of a parity of quantitative structure of neutral lipids and phospholipids, to preservation of permeability of membranes. The food supplement allocated of a guelder-rose can be used in a treatment and prophylactic feed.

[Biologically active additives in health prophylaxis of medical workers].

The aim of the investigation was to use the biologically active additive (BAD) Kalifen to prevent metabolic disturbances in medical workers during their occupational activity. Female physicians aged 40-50 years were examined. Group 1 (control) included 20 age-matched healthy female donors. Group 2 comprised 27 female physicians (anesthesiologists and surgeons) daily exposed to stresses (chemical, psychoemotional, work-related, etc.) who were asked after blood biochemical tests to daily take the BAD Kalifen in a dose of 2.5 ml after a meal (they made up Group 3), which is equal to the daily prophylactic dose in 100 mg of total polyphenols. The plasma tested before an experiment showed hypertriglyceridemia and hypercholesterolemia, suppressed hepatic etherifying function, imbalance of the fractional content of phospholipids, exhaustion of the antioxidative system, increased lipid peroxidation, and impaired erythrocytic membrane permeability. The prophylactic use of the BAD Kalifen eliminates stress-induced metabolic disturbances and increases occupational and biological longevity in the able-bodied population.

[Prevention of stress states in full-time students].

The study was undertaken to use the biologically active additive (BAA) Kalifen as a component of the jellies BIO-LAD for students' adaptation to academic load. Two groups of male volunteers aged 20-22 years were examined. Group 1 (control) included 20 healthy male donors of the comparable age. Group 2 comprised 10 students who were asked to take 100 mg of the jellies containing the BAA Kalifen (TU 9128-152-02067936-2006), which was equivalent to 100 mg of total polyphenols a day, on fasting every day after biochemical blood studies. The serum tested before the experiment showed hypertriglyceridemia and hypercholesterolemia, suppressed hepatic etherifying function and imbalance of the fractional content of phospholipids. Exhaustion of the antioxidative system, increased lipid peroxidation, and impaired erythrocytic membrane permeability were also ascertained. Supplementation of the jellies normalized the biochemical parameters under study. From the above discussion, it follows that the jellies BIO-LAD should be used as a purposefully acting product to prevent mental and emotional stress.

[Impact of the working environment on metabolic reactions in operating pilots].

Two groups of male volunteers aged 35-45 years were examined. Group 1 comprised 20 healthy donors controls) and Group 2 included 10 operating pilots whom after biochemical blood analysis were proposed to use daily the biologically active supplement (BAS) Kalifen, 5 ml (which was equal to 100 mg/day of total polyphenols) after meals in the morning for 4 weeks. Before the use of the supplement, the sera of the examinees provided evidence that they had hypertriglyceridemia and hypercholesterolemia, inhibited hepatic etherifying function, unbalanced phospholipid composition, depleted antioxidative system, enhanced lipid peroxidation, and increased erythrocytic membrane permeability. The combined influence of the working environment on the operating pilots's health status appeared as pronounced metabolic changes characteristic of those observed under physical and emotional stress. The use of Kalifen facilitated normalization of the studied biochemical parameters. From the afore-said, it is advisable to use BAS for the prevention of stress actions caused by a variety of physical and emotional agents.

[Impact of stress on hepatic lipid and carbohydrate metabolism, prevention].

An oligomer proantocyanidine complex (OPCC) isolated from the aqueous-and-alcoholic extract Kalifen and a polyphenol complex (PPC) isolated from the eleutherococcus extract were tested for their effects on carbohydrate and lipid metabolism in the rat liver under acute stress caused by hanging up of the animals by the cervical fold for 22 hours. This acute stress was accompanied by decreases in aerobic glycolysis and pentose cycle and by an increase in lipolysis. Hepatic etherifying function impaired. Lipids became predominant energy substrates. The administration of OPCC and PPC during stress promoted the preservation of carbohydrate metabolic reactions (gluconeogenesis, pentosephosphate pathway, Krebs cycle) and to the decrease of lipolysis. OPCC was shown to have stress-protective properties and to be able to diminish adrenal hypertrophy, to increase the production of oxidized equivalents (NAD+), and to enhance the activity of G-6-PDH in the rat liver. The complex is superior to the well-known antistress agent eleutherococcus, which makes it possible to regard these drugs as possible promising drugs in preventive and rehabilitative medicine.

[Stress-protector effect of diprim]. / Stress-protektivnoe deistvie diprima.

Diprim, a polyphenol preparation from crests of Far-Eastern grape species, influences the carbohydrate and lipid metabolism in liver of rats subjected to stress. The drug possesses antistress properties determined by the biologically active natural polyphenol derivatives. Playing the role of a buffer redox system under the stressed conditions, the polyphenol structures provide for the possibility of retaining the carbohydrate and lipid metabolism in rat liver and creating a reserve of the oxidized form of nicotinamide NAD+ coenzyme, which favors removal of the tissue hypoxia. The antioxidant properties of polyphenols from diprim preserve the phospholipid spectrum of the plasmic membranes of hepatocytes.

[Efficiency of Diprim for the restoration of the functional status of the liver after its damage by ethanol poisoning]. / Effektivnost' primeneniia rastitel'nogo preparata Diprim dlia vosstanovleniia funktsional'nogo sostoianiia pecheni posle porazheniia étilovym spirtom.

The effects of Diprim, a natural polyphenol complex isolated from Amur grapes crest, on lipid metabolism, lipid peroxidation (LPO), and antioxidative system of the liver were studied in ethanol-treated rats. Diprim injected to animals during ethanol abstinence arrested stress and thus inhibited lipolysis in fatty tissue and prevented triglyceride accumulation in the liver. Ethanol-suppressed metabolic reactions of the liver concerned with the synthesis of phospholipid fractions (phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine) were restored. Diprim promoted inhibition of LPO processes (the level of malonic dialdehyde decreased) and a pronounced glutathione-saving effect, which is largely due to its components--polyphenols which capture free radicals.

[Effects of a plant preparation Korrda-K on metabolic reactions in the liver of rats exposed to acetone]. / Vliianie rastitel'nogo preparata Korrda-K na metabolicheskie reaktsii v pecheni krys pri intoksikatsii atsetonom.

The paper deals with the study of the preventive effect of the plant drug Korrda-K on lipid and carbohydrate metabolism in the liver of the rat exposed to acetone inhalation. The agent was found to have hepatic and membranous protective effects. On exposure to acetone, Korrda-K promotes the normalization of blood glucose levels and other biochemical parameters. The magnitude of changes in the composition of lipids and fatty acids suggests that the preventive use of the agent favours the preservation of the membrane structure of hepatocytes changed by acetone.

[Effect of natural complexes of biologically active substances on liver regeneration in alcohol poisoning]. / Vliianie prirodnykh kompleksov biologicheski aktivnykh veshchestv na protsessy vosstanovleniia funktsii pecheni pri alkogol'noi intoksikatsii.

Hepatoprotective effect of natural substances obtained from extracts of grape combs, leaves of green and black tea were studied in liver tissue of ethanol-consuming rats by means of evaluation of the neutral lipid fractions and phospholipids as well as by measurement of glucose and nicotinamide coenzymes NAD+ and NADP in blood. In all the animal groups treated with these vegetable extracts content of total phospholipids, decreased after the ethanol treatment, was increased, while fraction composition of phospholipids and of neutral lipids was normalized; in blood content of glucose, NAD+ and NADP approached to control values. These results suggest that the vegetable extracts studied exhibited the hepatoprotective effect in alcohol intoxication.