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1.
J Ethnobiol Ethnomed ; 19(1): 8, 2023 Mar 25.
Article En | MEDLINE | ID: mdl-36964580

BACKGROUND: Proven toxicity and environmental burdens caused by artificial dyes have motivated dyeing industries to turn to natural alternatives. Plant-based dyestuffs are an interesting group of alternative crops. Reunion Island located in the Indian Ocean is the only European region in the southern hemisphere. It has a great number of assets to find new molecules in the abundant plant biodiversity. However, the dye-producing plants diversity in this island had not been documented to date. METHODOLOGY: The assessment of the Reunion Island's plant biodiversity through the "PLANTIN" project allowed us to establish here the first ethnobotanical inventory of plants growing on Reunion Island which may have promising properties as a new alternative source of dyes or colorants for the industries. First, an ethnobotanical survey focused on the uses of plants traditionally used in dyeing was conducted on local stakeholders. Then, the importance of different criteria (e.g., endemicity, accessibility and cultivability, plant organs used for the extraction, industrial interests of the species, etc.) has been considered to establish a classification method of the species, to finally select the most interesting plants which have been further harvested and investigated for their coloring property and dyeing application on natural fibers. RESULTS: The results showed that local people have accumulated traditional knowledge of dyeing plants, but that this approach had been discontinued in Reunion. The uses of 194 plant species potentially rich in dyes or pigments, belonging to 72 different families, with diverse botanical status (endemic, native, introduced or alien-invasive species) have been recorded. Then, 43 species were harvested and their coloring property were investigated. It demonstrated that dyes extracted from promising species, e.g., Terminalia bentzoe, Weinmannia tinctoria, Thespesia populnea, Erythroxylum laurifolium, Morinda citrifolia, Leea guinensis, Ochrosia borbonica, Danais fragrans, Terminalia cattapa, Casuarina equisetifolia, and Coccoloba uvifera, amongst others, could be used as new textile dyes. Their efficacy in the wool and cotton dyeing has been successfully demonstrated here. CONCLUSION: These plant-based dyestuffs showed promising coloring properties with different shades that could meet industrial application requirement. It's an area that could promote local cultural inheritance, create opportunity for business and farmers, and that can make a significant contribution to preserving endangered native species by supporting reforestation schemes. Additional researches are in progress to evaluate the safety of these plant-based colored extracts, their chemical composition and biological activities.


Coloring Agents , Ethnobotany , Humans , Reunion , Plant Extracts , Crops, Agricultural
2.
Arch Phys Med Rehabil ; 104(3): 372-379, 2023 03.
Article En | MEDLINE | ID: mdl-36030892

OBJECTIVE: To establish international recommendations for the management of spastic equinovarus foot deformity. DESIGN: Delphi method. SETTING: International study. PARTICIPANTS: A total of 24 international experts (N=24) in neuro-orthopedic deformities, from different specialties (Physical and Rehabilitation Medicine physicians, neurologists, geriatricians, orthopedic surgeons, neurosurgeons, plastic surgeons). INTERVENTIONS: Experts answered 3 rounds of questions related to important aspects of diagnosis, assessment, and treatment of spastic equinovarus foot deformity. MAIN OUTCOME MEASURES: A consensus was established when at least 80% of experts agreed on a statement RESULTS: A total of 52 items reached consensus. Experts recommend assessing effect of the deformity on functional activities before treatment. Before treatment, it is crucial to differentiate spastic muscle overactivity from soft tissue contractures, identify which muscles are involved in the deformity, and evaluate the activity of antagonist muscles. Motor nerve blocks, 2-dimensional video analysis, and radiologic examinations are often required to complement a clinical examination. The treatment of equinovarus foot depends on the correctability of the deformity and the patient's ability to stand or walk. The preoperative assessment should include an interdisciplinary consultation that must finalize a formal agreement between physicians and the patient, which will define personalized attainable goals before surgery. CONCLUSION: The establishment of guidelines on managing equinovarus foot will help physicians and surgeons, specialists, and nonspecialists to diagnoses and assess the deformity and direct patients to a network of experts to optimize patient functional recovery and improve their autonomy.


Clubfoot , Humans , Muscle Spasticity , Lower Extremity , Walking , Foot , Delphi Technique
3.
JPRAS Open ; 33: 171-183, 2022 Sep.
Article En | MEDLINE | ID: mdl-36051780

Background: Venous anatomy of the digits and the hand is poorly reported in the literature compared to arterial anatomy. While knowledge of the venous anatomy is crucial to ensure safe skin incisions, skin flap design, or blood return restoration for digital replantations, data in anatomical and clinical textbooks are rather limited. The purpose of this anatomical study was to describe the venous anatomy of the digits and the hand. Method: Our series reports descriptive results from 10 non-embalmed hand dissections from 5 different corpses. Hands were previously co-injected by arteries followed by veins with a different colored latex before being dissected under optical magnification (x4). Each anatomical specimen was photographed before being analyzed. Results: Each injection revealed both arterial and venous vascular systems. Latex injections were a useful technique to show the dorsal, volar superficial, and deep venous system. There was a constant and reliable topographic vascular anatomy of the superficial venous system of the digits and hand. However, we could not observe a high density of dorsal superficial venous valves as previously reported. Conclusion: The knowledge of the arrangement of the venous system of the digits and the hand should help the surgeon when performing surgical procedures in the hand. The surgeon should take into consideration this venous anatomy when performing skin incisions, skin flaps, or replantation procedures which would preserve the normal venous physiology as much as possible.

4.
Proc Inst Mech Eng H ; 235(3): 264-272, 2021 Mar.
Article En | MEDLINE | ID: mdl-33243076

Low back pain is a common, expensive, and disabling condition in industrialized countries. There is still no consensus for its ideal management. Believing in the beneficial effect of traction, we developed a novel external dynamic distraction device. The purpose of this work was to demonstrate that external distraction allows limiting the pressure exerted in standing-up position on the lower intervertebral discs. Numerical and cadaveric studies were used as complementary approaches. Firstly, we implemented the device into a numerical model of a validated musculoskeletal software (Anybody Modeling System) and we calculated the lower disc pressure while traction forces were applied. Secondly, we performed an anatomical study using a non-formalin preserved cadaver placed in a sitting position. A pressure sensor was placed in the lower discs under fluoroscopic control through a Jamshidi needle. The intradiscal pressure was then measured continuously at rest while applying a traction force of 200 N. Both numerical and cadaveric studies demonstrated a decrease in intradiscal pressures after applying a traction force with the external device. Using the numerical model, we showed that tensile forces below 500 N in total were sufficient. The application of higher forces seems useless and potentially deleterious. External dynamic distraction device is able to significantly decrease the intradiscal pressure in a sitting or standing position. However, the therapeutic effects need to be proven using clinical studies.


Intervertebral Disc , Low Back Pain , Humans , Lumbar Vertebrae , Pressure
5.
Surg Radiol Anat ; 43(5): 619-630, 2021 May.
Article En | MEDLINE | ID: mdl-33136183

INTRODUCTION: Typically, the axillary arch is defined as a fleshy slip running from latissimus dorsi to the anterior aspect of the humerus. Phylogeny seems to give the most relevant and plausible explanation of this anatomical variant as a remnant of the panniculus carnosus. However, authors are not unanimous about its origin. We report herein the incidence of axillary arch in a series of 40 human female dissections and present an embryologic and a comparative study in three domestic mammals. MATERIALS AND METHODS: Forty formalin-preserved Caucasian human female cadavers, one rat (Rattus norvegicus), one rabbit (Oryctolagus cuniculus) and one pig (Sus scrofa domesticus) cadavers were dissected bilaterally. A comparative, analytical and a descriptive studies of serial human embryological sections were carried out. RESULTS: We found an incidence of axillary arch of 2.5% (n = 1 subject of 40) in Humans. We found a panniculus carnosus inserted on the anterior aspect of the humerus only in the rat and the rabbit but not in the pig. The development of the latissimus dorsi takes place between Carnegie stage 16-23, but the embryological study failed to explain the genesis of the axillary arch variation. However, comparative anatomy argues in favour of a panniculus carnosus origin of the axillary arch. CONCLUSIONS: With an incidence of 2.5% of cases, the axillary arch is a relatively frequent variant that should be known by clinician and especially surgeons. Moreover, while embryology seems to fail to explain the genesis of this variation, comparative study gives additional arguments which suggest a possible origin from the panniculus carnosus.


Anatomic Variation , Humerus/abnormalities , Superficial Back Muscles/abnormalities , Aged , Aged, 80 and over , Animals , Cadaver , Dissection , Female , Humans , Middle Aged , Prospective Studies , Rabbits , Rats , Retrospective Studies , Sus scrofa
6.
Obesity (Silver Spring) ; 27(6): 950-956, 2019 06.
Article En | MEDLINE | ID: mdl-31006983

OBJECTIVE: A minipig model was employed to explore the changes in endogenous leptin transport into the central nervous system and in hypothalamic sensitivity to exogenous leptin when individuals are placed on high-fat diet (HFD) compared with standard diet. METHODS: Serum and cerebrospinal fluid (CSF) leptin concentrations during 10 weeks of HFD versus standard diet and exogenous leptin-induced STAT3 phosphorylation in the hypothalamus of minipigs were assessed, and the hypothalamic leptin-sensitive cells were characterized by immunofluorescence. RESULTS: The efficiency of the passage of endogenous blood-borne leptin into the CSF (measured as the log [CSF:serum leptin ratio]) decreased over time in minipigs fed a HFD (ß = -0.04 ± 0.005 per kilogram of weight gain in HFD; P < 0.0001), while it remained stable in minipigs fed a standard diet. However, the ability of peripherally administered leptin to activate its receptor in hypothalamic neurons was preserved in obese minipigs at 10 weeks of HFD. CONCLUSIONS: Together, these data are consistent with the existence of an early-onset tranport deficiency for endogenous circulating leptin into the brain in individuals developing obesity, preceding the acquisition of hypothalamic leptin resistance. Although additional studies are required to identify the underlying mechanisms, our study paves the way for the development of new preclinical pharmacological models targeting the restoration of the shuttling of peripheral leptin into the central nervous system to manage obesity.


Cerebrospinal Fluid/chemistry , Diet, High-Fat/adverse effects , Leptin/metabolism , Obesity/blood , Animals , Humans , Male , Swine , Swine, Miniature
7.
J Orthop ; 16(3): 216-219, 2019.
Article En | MEDLINE | ID: mdl-30906126

INTRODUCTION: The synovial wrist ganglion is a particularly common pathology of which the first "complication" is recidivism. The main objective of our study was to determine the rate of recurrence of this pathology in a series of arthroscopic patients. The secondary objective was to assess patient satisfaction well after the operation. MATERIAL AND METHODS: Our study was observational and retrospective and involved 30 patients (17 dorsal and 13 palmar cases) aged 41 years on average. The patients underwent an arthroscopic procedure for a palmar or dorsal ganglion of the wrist between March 2007 and April 2013. The data were collected by re-reading the files and conducting telephone interviews. Each patient answered a questionnaire about the operation, after-treatment, and their satisfaction well after the surgery. At the end of the interview, we calculated the Patient Rated Wrist Evaluation (PRWE) score. RESULTS: The mean follow-up was 4.6 years. A recurrence was noted in 4 (13%) cases, at an average delay of 9 months (6 months-1 year). There were only 2 patients (6.7%) that experienced the complication of complex regional pain syndrome type 1. Twenty-eight (93%) patients experienced improvement in postoperative pain. For 27 (90%) patients, firm-handed activities could be practised without limitation. The average time to resumption of activities of daily living was 27.1 days (1-240 days), resumption of firm-handed activities was 56 days (15-360 days), and return to work was 47.5 days (1-360 days). The mean PRWE score was 6.9/50 (0-34) for pain and 1.38/50 (0-8) for function. CONCLUSION: The 13% recurrence rate is on the average of what is observed in the literature. Later satisfaction with the intervention is very good, and complications remain rare.Studies tend to show a lower rate of complications and recurrence following arthroscopic treatment, but to date, no randomized comparative series between the two methods has yet revealed any significant difference in these two points. A study of this type on a large scale could make it possible to highlight one of these treatment approaches.

8.
Eur J Orthop Surg Traumatol ; 29(4): 785-792, 2019 May.
Article En | MEDLINE | ID: mdl-30649622

INTRODUCTION: Elbow dislocation can lead to instability and stiffness of the elbow. The main goal of this study was to list the initial elbow ligaments injuries caused by simple posterolateral elbow dislocations. The secondary goals were to assess ligament healing 2 months after the initial dislocation, to research a correlation between ligaments injuries and clinical course, and to search for predictive factors of instability. PATIENTS AND METHODS: Patients who had simple posterolateral elbow dislocation for the first time between January 2015 and May 2016 were included. Each patient had an MRI scan of their traumatised elbow on the day of the dislocation and then again 2 months later. The assessment was performed thanks to a clinical examination and calculation of functional recovery scores. The Mann-Whitney U test was used to research a correlation between the healing of ligaments injuries and clinical course. RESULTS: Twenty-five patients were included in the study. The initial MRI scans showed 70% and 54% ligament rupture, respectively, for the anterior band (ant MCL) and the posterior band (post MCL) of the medial collateral ligament (MCL), as well as 79% for the ulnar (ULCL) and 50% for the radial (RLCL) lateral collateral ligaments. The healing rate 2 months after dislocation was fairly low from 18% for the ULCL up to 41% for the anterior band of the MCL. No correlation was found between the ligament healing noticeable on MRI scans and clinical course. No elbow instability was diagnosed during the 4-month follow-up. CONCLUSION: Elbow dislocation is particularly damaging for ligaments. There is no predominance on medial or lateral ligament for rupture. The low healing rate 2 months after the initial dislocation could be explained by performing a follow-up MRI scan too early.


Collateral Ligaments/diagnostic imaging , Collateral Ligaments/injuries , Elbow Injuries , Elbow Joint/diagnostic imaging , Joint Dislocations/diagnostic imaging , Wound Healing , Adolescent , Adult , Disability Evaluation , Female , Follow-Up Studies , Humans , Joint Dislocations/therapy , Joint Instability/etiology , Magnetic Resonance Imaging , Male , Middle Aged , Prospective Studies , Range of Motion, Articular , Rupture/diagnostic imaging , Young Adult
9.
Knee Surg Sports Traumatol Arthrosc ; 27(4): 1241-1250, 2019 Apr.
Article En | MEDLINE | ID: mdl-30203198

PURPOSE: Few studies investigated whether trochlear and patellar design enhancements improve long-term outcomes of total knee arthroplasty (TKA). This study aimed to compare the long-term survival and complication rates of two consecutive generations of the same TKA system with identical tibiofemoral geometry, but different patellofemoral designs. METHODS: The authors retrieved the records of 93 patients (104 knees) operated with the HLS II system and 116 patients (122 knees) operated with HLS Evolution system. Patients were evaluated preoperatively and at a minimum of 10 years noting all complications. Kaplan-Meier (KM) survival was compared for two endpoints: (1) revision of all components and (2) revision of any component. RESULTS: From the HLS II series, the incidence of revision of all components was 6.4%, and of any component was 9.8%. From the HLS Evolution series, the incidence of revision of all components was 4.1%, and of any component was 5.1%. Comparing the survival at equivalent follow-up of 14 years, considering revision of all components, the HLS II had higher survival than the HLS Evolution (98.9% vs 95.9%), while considering revision of any component, the HLS II had lower survival than the HLS Evolution (93.0% vs 94.9%). The differences in survival of the two implants were not significant, neither at equivalent follow-up of 14 years (n.s.), nor at maximum follow-up of each cohort (n.s.). The complication rate was higher for the HLS II series compared to the HLS Evolution (28% vs 12%, p = 0.009), but patellofemoral complications were not more frequent (8% vs 6%, n.s.). CONCLUSIONS: Though the differences in survival of the two implants were not significant, conflicting findings are observed due to partial revisions for patellar fractures (5 in the HLS II series and 1 in the HLS Evolution series) which could be related to patellofemoral design enhancements. This study highlights the importance of patello-femoral geometry, which is often overlooked in TKA. LEVEL OF EVIDENCE: Retrospective comparative study, Level III.


Arthroplasty, Replacement, Knee/methods , Femur/surgery , Knee Joint/surgery , Knee Prosthesis , Patella/surgery , Postoperative Complications/prevention & control , Adult , Aged , Aged, 80 and over , Female , Femur/diagnostic imaging , Follow-Up Studies , France/epidemiology , Humans , Incidence , Knee Joint/diagnostic imaging , Knee Joint/physiopathology , Male , Middle Aged , Patella/diagnostic imaging , Postoperative Complications/epidemiology , Prognosis , Prosthesis Design , Retrospective Studies , Time Factors , Young Adult
11.
Surg Radiol Anat ; 41(4): 385-392, 2019 Apr.
Article En | MEDLINE | ID: mdl-30547208

PURPOSE: While anatomical variations of the subscapular vessels are frequently encountered during axillary dissection, little is found in the literature. The aim of this cadaveric study was to define arterial and venous anatomical variations and frequencies of the subscapular vascular pedicle and its terminal/afferent vessels in women. METHODS: We performed 80 dissections of the axillary region on forty female formalin-embalmed cadavers. Each anatomical arrangement was photographed and recorded on a scheme before analysis. RESULTS: We propose a new classification of the subscapular pedicle variations. We observed three types of subscapular arterial variation. The type Ia was the most frequent arrangement (71% of our dissections), the type Ib was observed in 11% and the type II in 18% of cases. We observed four types of subscapular venous variation. The type Ia was observed in 63% of cases, the type Ib in 14%, the type II in 14% and the type III in 10% of cases. CONCLUSIONS: This knowledge of the anatomical variation arrangement and frequencies of the subscapular vascular pedicle will assist the surgeon when dissecting the axillary region for malignant or reconstructive procedures.


Scapula/blood supply , Aged , Aged, 80 and over , Anatomic Variation , Axilla/blood supply , Breast Neoplasms/surgery , Cadaver , Female , Humans , Middle Aged , Prospective Studies
12.
Orthop Traumatol Surg Res ; 105(1): 153-158, 2019 02.
Article En | MEDLINE | ID: mdl-30591416

BACKGROUND: Tibialis posterior transfer (TPT) is the treatment most widely used to palliate foot drop due to dorsiflexor palsy. TPT has been extensively studied in patients with peripheral neurological causes of foot drop. In contrast, data are scarce on central foot drop, in which TPT is often blamed for causing flattening of the arches. The primary objective of this study was to assess the impact on foot alignment of TPT in patients with central foot drop. The secondary objective was to determine whether TPT combined with other surgical procedures improved gait. HYPOTHESIS: TTP can induce flattening of the medial arch of the foot. PATIENTS AND METHODS: We retrospectively identified 13 patients managed with TPT (1 foot per patient). Mean follow-up was 65 months (range, 12-108 months). The causes were stroke (n=5), head injury (n=3), spinal cord injury (n=2), cervical spondylotic myelopathy (n=1), cerebral palsy (n=1), and a brain tumour (n=1). The clinical assessment focused chiefly on forefoot alignment and footprint parameters. The following variables were collected from weight-bearing radiographs: Djian-Annonier angle, Méary-Toméno angle, lateral arch angle, and calcaneal pitch angle in the sagittal plane; talo-metatarsal angle in the transverse plane; and rearfoot valgus angle in the coronal plane. RESULTS: Of the 13 feet, 6 had normal footprint parameters and 7 pes cavus. There were no cases of flatfoot. Pronation deformities and supination deformities were each found in 2 patients. Comparing the radiographic parameters between the two feet in each patient identified differences only for the lateral arch angle and calcaneal pitch angle, which indicated pes cavus on the operated side (operated side: 142.7° [range, 136°-156°], p=0.041; and 24° [range, 14°-33°], p=0.028, respectively). DISCUSSION: In contrast to the working hypothesis, we found no evidence of progression to valgus flatfoot after TPT transfer performed to treat central foot drop. LEVEL OF EVIDENCE: IV, retrospective study with no control group.


Foot/diagnostic imaging , Peroneal Neuropathies/surgery , Tendon Transfer , Adult , Brain Neoplasms/complications , Calcaneus/diagnostic imaging , Cerebral Palsy/complications , Craniocerebral Trauma/complications , Female , Flatfoot/diagnostic imaging , Follow-Up Studies , Foot/pathology , Gait , Humans , Male , Metatarsal Bones/diagnostic imaging , Middle Aged , Peroneal Neuropathies/etiology , Peroneal Neuropathies/physiopathology , Radiography , Retrospective Studies , Spinal Cord Injuries/complications , Stroke/complications , Talipes Cavus/diagnostic imaging , Talus/diagnostic imaging , Time Factors , Tumor Protein, Translationally-Controlled 1
13.
J Craniomaxillofac Surg ; 46(12): 2256-2260, 2018 Dec.
Article En | MEDLINE | ID: mdl-30420152

PURPOSE: The purpose of our anatomical study was to compare the accessibility of the area at the level of the neck of the condyle and the condylar head by two different approaches: the modified Risdon approach and a transparotid approach by rhytidectomy (TPAR). METHODS: An anatomical study was performed on 12 heads of cadavers preserved with a solution rich in glycerin and ethanol, but very low in formalin. A modified Risdon approach and a TPAR were each performed on a hemiface of the same head. The bone surface was rendered digitally accessible on an anatomical diagram using Image J software. The software was used to determine a concordance score between the area of accessibility and the area of interest necessary for osteosynthesis of a high subcondylar fracture. This score was presented as a numerical scale from 0 to 100. We measured the total number of pixels in our area of interest and assigned it a score of 100. We then compared the area of accessibility with the area of interest necessary for osteosynthesis of a high subcondylar fracture. RESULTS: Using the modified Risdon approach, an average score of 55.88 (SD = 18.96) was found, or 55.88% of the accessible area of interest. Using TPAR, we found a score of 91.05 (SD = 7.95) or 91.05% of the accessible area of interest. This difference in score between the two techniques was significantly different (p < 0.001), taking into account intra-hemiface and intra-individual correlation. CONCLUSION: TPAR seems to be more effective in treating high condylar process fractures of the mandible.


Fracture Fixation, Internal/methods , Mandibular Condyle/anatomy & histology , Mandibular Condyle/surgery , Mandibular Fractures/surgery , Anatomic Landmarks , Cadaver , Humans , Mandibular Condyle/injuries
14.
Surg Radiol Anat ; 40(8): 903-910, 2018 Aug.
Article En | MEDLINE | ID: mdl-29978328

INTRODUCTION: The trapezius muscle is a good option for soft tissue defects in the cervical and thoracic regions. However, pedicled flaps raised from perforators in the trapezius are rare. Through a series of cadaver dissections, the authors aim to map the perforating arteries in the trapezius muscle. MATERIALS AND METHODS: Dissection of 58 anatomical regions in 32 cadavers was carried out. The perforating arteries of the trapezius were inventoried based on their location, dimensions and frequency using the scapular spine and spinous process line as landmarks. RESULTS: In 78% of cases, the main perforators of the trapezius were located along two vertical lines parallel to the spinous process line, 5-6 cm on either side of it, between the scapular spine laterally, the tip of the scapula caudally and the superior margin of the trapezius cranially. On each side of the spinous process line, we found an average of 16 perforators (range 5-27) with an average diameter of 0.6 mm (range 0.1-2.6 mm). CONCLUSION: Our study shows that surgeons can use fixed landmarks when developing trapezius perforator flaps.


Anatomic Landmarks , Arteries/anatomy & histology , Perforator Flap/blood supply , Plastic Surgery Procedures/methods , Superficial Back Muscles/blood supply , Adult , Aged , Aged, 80 and over , Cadaver , Dissection , Female , Humans , Male , Middle Aged , Neck/surgery , Perforator Flap/transplantation , Superficial Back Muscles/transplantation , Thoracic Wall/surgery
15.
Article En | MEDLINE | ID: mdl-29777859

OBJECTIVE: The anatomy of the perineum and the pelvic diaphragm of woman is complex. A numerical complete three-dimensional (3D) model of every muscle of the woman pelvis doesn't exist. The pathophysiology of genital prolapse is still debated. Knowledge of anatomy is essential to better understand its mechanisms. The aim of this research was to build a complete three-dimensional model of the female perineum and pelvic floor muscles. STUDY DESIGN: To model the pelvic muscles we reconstructed them in three dimensions from tracing a magnetic resonance imaging (MRI) of a female pelvis from a cadaver. RESULTS: We obtained a complete anatomical model of the muscles of the perineum and pelvic floor. Each muscle was built thanks to the MRI. CONCLUSION: We obtained the first complete anatomical model of the perineal muscles and pelvic diaphragm. It could be a good educational and simulation tool for better understanding normal and pathological pelvic mobility.


Imaging, Three-Dimensional , Models, Anatomic , Muscle, Skeletal/anatomy & histology , Pelvic Floor/anatomy & histology , Perineum/anatomy & histology , Female , Humans , Magnetic Resonance Imaging , Muscle, Skeletal/diagnostic imaging , Pelvic Floor/diagnostic imaging , Perineum/diagnostic imaging
16.
J Comp Neurol ; 526(9): 1419-1443, 2018 06 15.
Article En | MEDLINE | ID: mdl-29230807

The adult brain contains niches of neural stem cells that continuously add new neurons to selected circuits throughout life. Two niches have been extensively studied in various mammalian species including humans, the subventricular zone of the lateral ventricles and the subgranular zone of the hippocampal dentate gyrus. Recently, studies conducted mainly in rodents have identified a third neurogenic niche in the adult hypothalamus. In order to evaluate whether a neural stem cell niche also exists in the adult hypothalamus in humans, we performed multiple immunofluorescence labeling to assess the expression of a panel of neural stem/progenitor cell (NPC) markers (Sox2, nestin, vimentin, GLAST, GFAP) in the human hypothalamus and compared them with the mouse, rat and a non-human primate species, the gray mouse lemur (Microcebus murinus). Our results show that the adult human hypothalamus contains four distinct populations of cells that express the five NPC markers: (a) a ribbon of small stellate cells that lines the third ventricular wall behind a hypocellular gap, similar to that found along the lateral ventricles, (b) ependymal cells, (c) tanycytes, which line the floor of the third ventricle in the tuberal region, and (d) a population of small stellate cells in the suprachiasmatic nucleus. In the mouse, rat and mouse lemur hypothalamus, co-expression of NPC markers is primarily restricted to tanycytes, and these species lack a ventricular ribbon. Our work thus identifies four cell populations with the antigenic profile of NPCs in the adult human hypothalamus, of which three appear specific to humans.


Hypothalamus/anatomy & histology , Neural Stem Cells/physiology , Stem Cell Niche/physiology , Animals , Biological Ontologies , Doublecortin Domain Proteins , Humans , Lemur , Mice , Microtubule-Associated Proteins/metabolism , Neuropeptides/metabolism , Rats , Species Specificity
17.
J Plast Reconstr Aesthet Surg ; 70(6): 781-791, 2017 Jun.
Article En | MEDLINE | ID: mdl-28259642

INTRODUCTION: The deep inferior epigastric perforator (DIEP) flap is a reliable and reproducible technique for autologous microsurgical breast reconstruction. Several recipient vessels sites for microvascular anastomosis have been described such as the internal thoracic vessels, the thoracodorsal vessels, and the circumflex scapular vessels. Nonetheless, the choice of the recipient site depends mainly on individual operator's experience and preferences, and currently the best recipient vessel site is under debate. This anatomical observational study aimed to determine whether anatomy could address this dilemma by determining the best vessel diameter to match the donor with these three recipient sites. METHODS: Our series reports 80 dissections of the three anatomical regions of interest. Forty formalin-preserved female cadavers were dissected bilaterally. Internal vessels diameter measurements were recorded with a vascular gauge ranging from 1.0 to 5.0 mm with successive half-millimeter graduations. RESULTS: The median diameter of the deep inferior epigastric (DIEA), internal thoracic (ITA), circumflex scapular (CSA), and thoracodorsal arteries (TDA) were: 2.0, 2.5, 2.5, and 1.5 mm, respectively. The median diameter of the deep inferior epigastric, internal thoracic, circumflex scapular, and thoracodorsal veins were: 3.0, 3.0, 3.0, and 2.5 mm, respectively. At the individual level, the perfect match between DIEA and ITA was significantly more frequent than between DIEA and TDA (p = 0.002), and it was more frequent between DIEA and CSA than between DIEA and TDA (p = 0.009). CONCLUSIONS: This study supports the use of the internal thoracic pedicle as the first recipient vessel choice, which should be considered, at least anatomically, as the best one with the closest diameter matching with the donor pedicle.


Mammaplasty/methods , Microsurgery/methods , Perforator Flap/blood supply , Thoracic Arteries/anatomy & histology , Thorax/blood supply , Veins/anatomy & histology , Aged , Aged, 80 and over , Cadaver , Epigastric Arteries/anatomy & histology , Female , Humans , Middle Aged
18.
Hand Surg Rehabil ; 35(1): 44-50, 2016 02.
Article En | MEDLINE | ID: mdl-27117024

Comminuted radial head fractures are usually associated with destabilizing lesions of the elbow. The radial head prosthesis (RHP) is a therapeutic alternative when fracture fixation is impossible, as it restores one of the elbow's secondary stabilizers. The aim of this study was to assess healing of the medial collateral ligament (MCL) after implantation of a RHP. All patients implanted with a RHP during the 2003-2012 period were eligible for follow-up ultrasound evaluation of MCL healing. A clinical evaluation with standardized tests (DASH and MEPS) was performed and the patients asked to evaluate their elbow's stability during the same follow-up visit. Out of the 33 eligible patients, 20 were included with a mean follow-up of 73 months. The MCL had been torn in 15 of these 20 patients; the MCL appeared healed on the ultrasound images in 13 patients. The elbow was considered subjectively stable in 19 patients. The mean DASH score was 21.4 (±16.67) and the mean MEPS was 84.7 (±13.9); these scores were 17.19 (±14.0) and 88.3 (±10.9) for patients with a healed MCL, versus 32.5 (±10.6) and 70 (±0.0) for patients with a non-healed MCL. Our findings suggest that a torn MCL can heal after RHP implantation, even without surgical ligament repair or reattachment.


Arthroplasty/methods , Collateral Ligaments/injuries , Fractures, Comminuted/surgery , Prosthesis Implantation , Radius Fractures/surgery , Radius/surgery , Wound Healing , Adult , Arthroplasty/instrumentation , Collateral Ligaments/surgery , Elbow Joint/physiology , Female , Follow-Up Studies , Humans , Male , Retrospective Studies , Time Factors
19.
Hand Surg Rehabil ; 35(2): 100-6, 2016 04.
Article En | MEDLINE | ID: mdl-27117123

Proximal row carpectomy (PRC) and four-corner arthrodesis (4CA) are the two most commonly performed surgical procedures to treat wrist arthritis. Postoperative strength is one of the criteria for choosing between the two techniques. Some authors believe that strength is correlated with residual carpal height. The goal of this study was to determine if postoperative carpal height was predictive of postoperative strength. This study consisted of two parts: a clinical evaluation of grip strength after 4CA or PRC; anatomical and radiological measurements of carpal height before and after 4CA or PRC. Grip strength was better preserved after PRC (87.5%) than after 4CA (76.1%), when expressed relative to the opposite hand (P=0.053). There was a significant decrease in carpal height for the PRC group with a Youm's index of 0.37 versus 0.50 for the 4CA group (P<0.0001). Our clinical results and analysis of the literature indicate that 4CA is not superior to PRC when it comes to grip strength, whereas carpal height is significantly decreased after PRC. The decreased tendon excursion after PRC is balanced by an increase in joint stresses after 4CA.


Arthritis/surgery , Arthrodesis/methods , Carpal Bones/anatomy & histology , Carpal Bones/surgery , Hand Strength , Wrist Joint/surgery , Adult , Aged , Arthritis/diagnostic imaging , Biomechanical Phenomena , Carpal Bones/diagnostic imaging , Female , Humans , Male , Middle Aged , Range of Motion, Articular , Scaphoid Bone/diagnostic imaging , Scaphoid Bone/surgery , Treatment Outcome , Wrist Joint/diagnostic imaging , Young Adult
20.
Surg Radiol Anat ; 38(4): 395-401, 2016 May.
Article En | MEDLINE | ID: mdl-26589680

PURPOSE: Knowledge of elbow kinematics is essential to better understand this joint. There is currently no reliable dynamic method to accurately study the elbow joint in a non-invasive manner. The goal of this study was to implement an accurate protocol to study in vivo elbow kinematics using a VICON™ optoelectronic motion analysis system. MéTHODS: The elbow's centers of rotation (CR) were calculated for 10 anatomical specimens. The effect of skin movement was determined by comparing measurements taken using skin surface markers and bone-fixed markers. The validated protocol was then used in 30 healthy subjects who underwent passive elbow joint movements. RESULTS: The elbow's CR was found to be distal (7 ± 14 mm), lateral (4 ± 9 mm) and anterior (4 ± 10 mm) to the medial epicondyle in vitro. Mean CR values for anatomical specimens did not differ whether calculated using the skin-based or bone-fixed markers. CONCLUSION: This study has validated a dynamic, non-invasive, and accurate method for locating the elbow's center of rotation. This preliminary study thus found a different center of rotation of the one in the middle of the trochlea previously thought. This could lead us to reflect on the designs of our prostheses to reduce the mechanical stresses and the risk of loosening.


Elbow Joint/physiology , Adult , Biomechanical Phenomena , Female , Humans , Male , Young Adult
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