Cryopreservation of seeds of Neonotonia wightii Wight and Arn: a strategy for conservation, dormancy breaking and preservation of nutritional status.

BACKGROUND: N. wightii (Leguminosae) is valued as a cover crop and as a potential source of protein in food insecure countries. However, plantlet establishment is limited by physical dormancy. Our previous work has shown that exposure of N. wightii seeds to cryogenic temperatures is able to overcome physical dormancy. OBJECTIVE: The current study is an extension of that work where the field performance and nutritional composition of plants regenerated from N. wightii seeds was investigated. RESULTS: It was evident that plants regenerated from cryopreserved seeds displayed faster growth rates than those from control seeds. In addition, cryopreservation did not alter the nutritional profile of plants produced from cryo-stored seeds. CONCLUSION: Collectively, the results indicate that cryopreservation serves as a suitable strategy for the preservation of seeds of N. wightii with the added benefit of also serving as a dormancy breaking mechanism upon retrieval from cryogenic temperatures. Doi.org/10.54680/fr23510110712.

Exposure of Teramnus labialis (L.F.) Spreng Seeds to Liquid Nitrogen Does Not Affect Nutritional Status of Field Grown Adult Plants.

BACKGROUND: Teramnus labialis (L.f.) Spreng is a potentially important legume species, and can be used as an animal feed and to enhance soil physicochemical characteristics. Despite the biological and agricultural importance, the low availability of seeds, their small size and the low percentage germination limit their large-scale use by farmers. We previously reported a method to cryopreserve seeds of T. labialis which also allowed for the breaking of seed dormancy. OBJECTIVE: The current study reports on the nutritional status of 5 month old field grown plants regenerated from cryostored and control seeds. MATERIALS AND METHODS: Biomass (fresh and dry mass of leaves and stems) and contents of ash, neutral detergent fiber, acid detergent fiber, lignin, cellulose, crude protein, P, Ca, Mg and K were measured. RESULTS: Seeds germinated and emerged faster following immersion in liquid nitrogen (LN) which was supported by quantitative evaluations of fresh and dry weights per m2. However, the ratio of leaf:stem mass were not altered by seed exposure to LN. CONCLUSION: The results showed that exposure of seeds to cryogenic temperatures did not alter the nutritional composition of regenerated plants.

Pr-magnetism in the quasi-skutterudite compound PrFe2Al8.

The intermetallic compound PrFe2Al8 that possesses a three-dimensional network structure of Al polyhedra centered at the transition metal element Fe and the rare earth Pr is investigated through neutron powder diffraction and inelastic neutron scattering in order to elucidate the magnetic ground state of Pr and Fe and the crystal field effects of Pr. Our neutron diffraction study confirms long-range magnetic order of Pr below [Formula: see text] K in this compound. Subsequent magnetic structure estimation reveals a magnetic propagation vector [Formula: see text] with a magnetic moment value of [Formula: see text]/Pr along the orthorhombic c-axis and evidence the lack of ordering in the Fe sublattice. The inelastic neutron scattering study reveals one crystalline electric field excitation near 19 meV at 5 K in PrFe2Al8. The energy-integrated intensity of the 19 meV excitation as a function of [Formula: see text] follows the square of the magnetic form factor of [Formula: see text] thereby confirming that the inelastic excitation belongs to the Pr sublattice. The second sum rule applied to the dynamic structure factor indicates only 1.6(2) [Formula: see text] evolving at the 19 meV peak compared to the 3.58 [Formula: see text] for free [Formula: see text], indicating that the crystal field ground state is magnetic and the missing moment is associated with the resolution limited quasi-elastic line. The magnetic order occurring in Pr in PrFe2Al8 is counter-intuitive to the symmetry-allowed crystal field level scheme, hence, is suggestive of exchange-mediated mechanisms of ordering stemming from the magnetic ground state of the crystal field levels.

Anxiety and depression in parents of a Brazilian non-clinical sample of attention-deficit/hyperactivity disorder (ADHD) students

Higher prevalence rates of anxiety and depression have been reported in parents of children with attention-deficit/hyperactivity disorder (ADHD). The interaction between the burden of ADHD in offspring, a higher prevalence rate of this highly inherited disorder in parents, and comorbidities may explain this finding. Our objective was to investigate levels of ADHD, anxious and depressive symptomatology, and their relationship in parents of ADHD children from a non-clinical sample using a dimensional approach. The sample included 396 students enrolled in all eight grades of a public school who were screened for ADHD using the SNAP IV rating scale. Positive cases were confirmed through a semi-structured interview. Parents of all 26 ADHD students and 31 paired controls were enrolled. A sample of 36 parents of ADHD children (21 mothers, 15 fathers) and 30 parents of control children (18 mothers, 12 fathers) completed the Adult Self Report Scale, State-Trait Anxiety Inventory, and Beck Depression Inventory in order to investigate anxious and depressive symptomatology. Probands' mothers presented a higher level of ADHD symptomatology (with only inattention being a significant cluster). Again, mothers of ADHD children presented higher depressive and anxiety levels; however, these did not correlate with their own ADHD symptomatology. Only trait-anxiety levels were higher in ADHD mothers. Our findings suggest that: 1) anxious and depressive symptoms might be more prevalent in mothers of ADHD students; 2) anxious and depressive symptomatology might be independent of impairment associated with ADHD symptoms; 3) anxious and depressive symptoms are independent of the presence of ADHD.

Anxiety and depression in parents of a Brazilian non-clinical sample of attention-deficit/ hyperactivity disorder (ADHD) students.

Higher prevalence rates of anxiety and depression have been reported in parents of children with attention-deficit/hyperactivity disorder (ADHD). The interaction between the burden of ADHD in offspring, a higher prevalence rate of this highly inherited disorder in parents, and comorbidities may explain this finding. Our objective was to investigate levels of ADHD, anxious and depressive symptomatology, and their relationship in parents of ADHD children from a non-clinical sample using a dimensional approach. The sample included 396 students enrolled in all eight grades of a public school who were screened for ADHD using the SNAP IV rating scale. Positive cases were confirmed through a semi-structured interview. Parents of all 26 ADHD students and 31 paired controls were enrolled. A sample of 36 parents of ADHD children (21 mothers, 15 fathers) and 30 parents of control children (18 mothers, 12 fathers) completed the Adult Self Report Scale, State-Trait Anxiety Inventory, and Beck Depression Inventory in order to investigate anxious and depressive symptomatology. Probands' mothers presented a higher level of ADHD symptomatology (with only inattention being a significant cluster). Again, mothers of ADHD children presented higher depressive and anxiety levels; however, these did not correlate with their own ADHD symptomatology. Only trait-anxiety levels were higher in ADHD mothers. Our findings suggest that: 1) anxious and depressive symptoms might be more prevalent in mothers of ADHD students; 2) anxious and depressive symptomatology might be independent of impairment associated with ADHD symptoms; 3) anxious and depressive symptoms are independent of the presence of ADHD.

Evaluacion del indice de riesgo de infeccion quirurgica (IRIQ) en la cirugia pediatrica / Evaluation of infection rate in pediatric surgical operations

La incidencia de infecciones en cirugia pediatrica varya en la literatura internacional de 7,5 por ciento a 30 por ciento, no existiendo una diferencia significativa con los pacientes adultos. Las cifras tan variables dependen de las caracteristicas de la poblacion y de los procedimientos especificos. La clasificacion de los procedimientos por la potencialidad de contaminacion, la evaluacion del estado clinico en base a los criterios de la American Society of Anestesiology (ASA) y el tiempo de duracion de las cirugias, son las formas tradicionales de evaluar estos riesgos. El objetivo de este estudio fue evaluar el indice de Riesgo de Infeccion Quirurgica (IRIQ), propuesto por el Centers of Diseases Control and Prevention (CDC), en tres franjas etarias de la cirugia pediatrica. Fueron evaluados 773 pacientes sometidos a cirugia pediatrica en el periodo de Enero de 1993 a Diciembre de 1994. Las infecciones fueron diagnosticadas mediante una busqueda activa intra y extra hospitalaria, utilizando los criterios del CDC. La tasa de infeccion quirurgica fue del 3,5 por ciento (27/773). El potencial de contaminacion, el ASA y el tiempo de cirugia aisladamente fueron buenos predictores de riesgo de infeccion quirurgica. La asociacion de las tres variables fue mas adecuada y con menos variaciones que la relacion con la franja etaria. Recomendamos que el analisis de incidencia de infeccion en cirugia pediatrica sea realizado a traves de estadificaciones por el IRIQ

Evaluacion del indice de riesgo de infeccion quirurgica (IRIQ) en la cirugia pediatrica / Evaluation of infection rate in pediatric surgical operations

La incidencia de infecciones en cirugia pediatrica varya en la literatura internacional de 7,5 por ciento a 30 por ciento, no existiendo una diferencia significativa con los pacientes adultos. Las cifras tan variables dependen de las caracteristicas de la poblacion y de los procedimientos especificos. La clasificacion de los procedimientos por la potencialidad de contaminacion, la evaluacion del estado clinico en base a los criterios de la American Society of Anestesiology (ASA) y el tiempo de duracion de las cirugias, son las formas tradicionales de evaluar estos riesgos. El objetivo de este estudio fue evaluar el indice de Riesgo de Infeccion Quirurgica (IRIQ), propuesto por el Centers of Diseases Control and Prevention (CDC), en tres franjas etarias de la cirugia pediatrica. Fueron evaluados 773 pacientes sometidos a cirugia pediatrica en el periodo de Enero de 1993 a Diciembre de 1994. Las infecciones fueron diagnosticadas mediante una busqueda activa intra y extra hospitalaria, utilizando los criterios del CDC. La tasa de infeccion quirurgica fue del 3,5 por ciento (27/773). El potencial de contaminacion, el ASA y el tiempo de cirugia aisladamente fueron buenos predictores de riesgo de infeccion quirurgica. La asociacion de las tres variables fue mas adecuada y con menos variaciones que la relacion con la franja etaria. Recomendamos que el analisis de incidencia de infeccion en cirugia pediatrica sea realizado a traves de estadificaciones por el IRIQ