RESUMEN
BACKGROUND/AIMS: Oxidative stress plays an important role in liver fibrosis. Under pathological conditions, hepatic stellate cells (HSC) undergo an activation process, developing a myofibroblast-like phenotype from the lipocyte phenotype. In this study, we determined the levels of oxidative stress and proliferation in different activation states of an experimental model of mouse HSC, the GRX cell line. These cells can be induced in vitro to display a more activated state or a quiescent phenotype. METHODS/RESULTS: We observed increased oxidative damage and higher levels of reactive oxygen species, measured by thiobarbituric acid reactive species and 2',7'-dichlorofluorescein diacetate, respectively, and diminished catalase activity in activated cells. Activation decreased proliferation and increased the number of cells in G2/M. Antioxidants N-acetylcysteine and Trolox varied in their capacity to correct the oxidative stress and proliferation status. CONCLUSIONS: The differences in physiological functions of stellate cell phenotypes suggest a relationship between oxidative stress levels and activation state.
Asunto(s)
Cirrosis Hepática/fisiopatología , Hígado/citología , Hígado/fisiología , Estrés Oxidativo/fisiología , Acetilcisteína/farmacología , Animales , Antioxidantes/farmacología , Catalasa/metabolismo , Línea Celular , Proliferación Celular/efectos de los fármacos , Cromanos/farmacología , Citocinas/farmacología , Hígado/efectos de los fármacos , Cirrosis Hepática/patología , Ratones , Estrés Oxidativo/efectos de los fármacos , Fenotipo , Especies Reactivas de Oxígeno/metabolismo , Factor de Necrosis Tumoral alfa/farmacología , Vitamina E/farmacologíaRESUMEN
Rotavirus are the major etiological agent of acute gastroenteritis in various species of mammals and birds. This study was aimed to explore the frequency of rotavirus in flocks of commercial laying hens and industrial broiler chicken. Feces and caecal content were sampled from 589 birds belonging to 17 farms located in the neighbourhood of Lujan National University. RNA patterns were explored by polyacrylamide gel electrophoresis. Viral particles were found with moderate frequency throughout the period of study: 7.06% in 1987; 7.45% in 1988; 12.57% in 1989. This finding demonstrates the permanence of rotavirus infection in the farm under study and emphasizes the importance of routine rotavirus screening in industrial poultry farms.
Asunto(s)
Pollos/microbiología , Gastroenteritis/veterinaria , Enfermedades de las Aves de Corral/epidemiología , Infecciones por Rotavirus/veterinaria , Rotavirus/aislamiento & purificación , Crianza de Animales Domésticos/métodos , Animales , Argentina/epidemiología , Electroforesis en Gel de Poliacrilamida , Heces/microbiología , Femenino , Gastroenteritis/epidemiología , Incidencia , Tamizaje Masivo , Enfermedades de las Aves de Corral/microbiología , ARN Viral/análisis , Infecciones por Rotavirus/epidemiología , Estaciones del AñoRESUMEN
Rotavirus are the major etiological agent of acute gastroenteritis in various species of mammals and birds. This study was aimed to explore the frequency of rotavirus in flocks of commercial laying hens and industrial broiler chicken. Feces and caecal content were sampled from 589 birds belonging to 17 farms located in the neighbourhood of Lujan National University. RNA patterns were explored by polyacrylamide gel electrophoresis. Viral particles were found with moderate frequency throughout the period of study: 7.06
in 1987; 7.45
in 1988; 12.57
in 1989. This finding demonstrates the permanence of rotavirus infection in the farm under study and emphasizes the importance of routine rotavirus screening in industrial poultry farms.