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1.
BMJ Open Sport Exerc Med ; 10(3): e001940, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39161556

RESUMEN

Background: Injury and illness surveillance helps establish the infrastructure to provide adequate medical support at regattas and is the foundation for developing prevention strategies. Objectives: To assess the prevalence and characteristics of injuries in the 4 weeks before the start of the 2022 World Rowing Beach Sprints Finals (WRBSF) (the 'Prevalence Survey') and describe the incidence and nature of new-onset injuries and illnesses incurred during the WRBSF (the 'Incidence Survey'). Methods: Athletes completed: (1) a prevalence survey recording injuries the 4 weeks before the WRBSF and (2) a recording of injuries and illnesses that occurred during the 3-day regatta. Results: Fifty-nine of 152 eligible WRBSF athletes completed the prevalence injury survey. Twenty-three (38.9%) reported experiencing at least one injury within the 4 weeks before the WRBSF. The most prevalent anatomical injury sites were the forearm (11.86%), lumbar spine (10.17%), knee (8.47%), ankle (6.78%) and hand/fingers (6.78%). During the competition, only two illness occurrences were reported; both were respiratory infections. Two athletes reported injuries: a hamstring strain and a concussion. Conclusion: Rowers presenting to the WRBSF described injuries leading up to the event that were similar to those common in classic rowing. Rowers at the event suffered injuries of the lower limb that were different from classic rowing and may be related to the addition of running to this event. An event concussion should be considered as a more likely injury in this type of rowing and future events should be prepared to manage such an injury.

2.
Front Sports Act Living ; 6: 1441022, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39189032

RESUMEN

One of the reoccurring questions that arises during the countermovement vertical jump (CVJ) assessment is whether the learning effect has an impact on the accuracy of the results obtained. Thus, the purpose of the present investigation was to examine the impact of the short-term learning effect on the assessment of lower-body neuromuscular performance characteristics when performed on a portable one-dimensional force plate system. Sixteen recreationally active college-age males volunteered to participate in the present study. Each participant completed four sets of three non-consecutive CVJs with no arm swing throughout a single day. Besides strong verbal encouragement, participants were constantly instructed to focus on pushing the ground as explosively as possible. Fourteen force-time metrics were selected for CVJ performance analysis purposes: eccentric and concentric peak and mean force and power, eccentric and concentric duration, contraction time, jump height, reactive strength index-modified, and countermovement depth. Repeated measures multivariate analysis of variance was used to examine statistically significant differences across four testing time points (p < 0.05). The results indicate an absence of any meaningful differences across four testing time points in force-time metrics of interest during both eccentric and concentric phases of the CVJ. Moreover, no differences were observed in CVJ outcome metrics such as countermovement depth, suggesting that the movement strategy tends to remain consistent. Overall, these findings reveal that CVJ test repeatability is not affected by the short-term learning effect and that data are stable at least within the scope of this study and within this population.

3.
Front Sports Act Living ; 6: 1439858, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39193491

RESUMEN

Sleep has been recognized as one of the most essential recovery methods necessary for achieving optimal performance. However, there is still a lack of scientific literature focused on examining its impact on one of the most prevalent skills in the game of basketball, the countermovement vertical jump (CVJ). Therefore, the purpose of the present study was to examine the relationship between sleep quality and quantity, and lower-body neuromuscular performance characteristics within a cohort of semi-professional male basketball players. Twenty-eight athletes competing in a first-tier regional league in Serbia volunteered to participate in this investigation. Upon arrival at the gym, all athletes completed the Pittsburgh Sleep Quality Index (PSQI) self-rated questionnaire. Immediately after completion of the PSQI, each athlete stepped on a force plate system and performed three maximum-effort CVJs with no arm swing. The following force-time metrics were obtained for the analysis: eccentric and concentric absolute and relative mean and peak force and power, vertical jump height, and reactive strength index-modified. Pearson product-moment correlation coefficients were used to examine the strength of the linear relationships between sleep quality and quantity and lower-body neuromuscular performance characteristics (p < 0.05). The results indicated that sleep quality appears to have a greater impact on the concentric than the eccentric phase of the CVJ (e.g., concentric mean force [r = -0.830; p < 0.001], relative concentric peak force [r = -0.466; p = 0.013, eccentric mean power (r = -0.162; p = 0.409)], while no significant relationship was found between sleep quantity and lower-body neuromuscular performance (e.g., concentric peak force [r = -0.055; p = 0.782], relative eccentric mean power [r = -0.301; p = 0.107]). Overall, these findings offer valuable insights into the importance of good sleep hygiene (e.g., efficiency, duration) in an athletic population, and can help practitioners develop more effective training and recovery programs.

4.
Front Sports Act Living ; 6: 1384476, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39011348

RESUMEN

Introduction: With recent increases in the popularity of studying the physical construct of horizontal deceleration performance in team-sport athletes, the aim of the present study was to assess the inter-rater and intra-rater reliability of processing and quantifying horizontal deceleration ability using radar technology. Methods: Data from 92 NCAA Division 1 athletes from two different athletic teams (American football and Lacrosse) were used for the present investigation. All athletes performed two trials of the modified acceleration to deceleration assessment (ADA), which consisted of a maximal 10 m sprint acceleration, followed by a rapid deceleration. Four individual raters manually processed raw, radar-derived instantaneous velocity data for the ADA, and an automated script was used to calculate metrics of interest. Results: Primary study findings suggest moderate to excellent levels of agreement (ICC = 0.56-0.91) for maximal horizontal deceleration metrics between the four individual raters. The intra-rater analyses revealed poor to excellent consistency (ICC = 0.31-0.94) between ADA trials, with CV%'s ranging from 3.1% to 13.2%, depending on the respective metric and rater. Discussion: Our data suggests that if a foundational understanding and agreement of manual data processing procedures for radar-derived data is given between raters, metrics may be interpreted with moderate to excellent levels of confidence. However, when possible, and when using the Stalker ATS radar technology, authors recommend that practitioners use one trained individual to manually process raw data. Ideally, this process should become fully automated, based on selected filters or algorithms, rather than the subjectivity of the rater.

5.
J Strength Cond Res ; 2024 Jul 23.
Artículo en Inglés | MEDLINE | ID: mdl-39074242

RESUMEN

ABSTRACT: Cabarkapa, DV, Fry, AC, Kavadas, NG, and Cabarkapa, D. Are load-velocity estimates of bench press maximal strength as accurate as actual 1-repetition maximum testing? J Strength Cond Res XX(X): 000-000, 2024-The purpose of the present investigation was to determine if using maximal velocity measures while lifting submaximal loads as a predictor of bench press maximal strength (i.e., 1 repetition maximum [1RM]) is more accurate than the actual 1RM test and determine which specific submaximal loads best estimate 1RM bench press strength with the lowest variability when compared with actual 1RM tests. Sixteen recreationally trained subjects performed 5 testing sessions. The first and second sessions included the actual 1RM bench press testing, whereas the remaining 3 sessions consisted of performing one repetition of a bench press exercise in a series of incremental loads, starting at 20% 1RM and increasing the resistance by 10% until reaching the 90% of individual's 1RM. For each participant, linear regressions using bar velocities at each relative load were used to estimate 1RM capabilities, using the predetermined 1RM barbell velocities from actual 1RM testing. The results of the present investigation indicated the following: (a) actual bench press 1RM can be a highly reliable assessment of maximal strength; (b) having a greater number of loads included in the equations increases the accuracy of 1RM estimation; (c) practitioners should incorporate light (e.g., 20% 1RM) and heavy (e.g., 80 and/or 90% 1RM) loads when estimating 1RM from load-velocity profiles; and (d) most load-velocity regression equations for estimating strength are not as accurate as actual 1RM tests for the free-weight bench press. Those who use load-velocity testing to estimate 1RM strength must be willing to accept the accompanying error for most loading protocols.

6.
Front Sports Act Living ; 6: 1399399, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38887688

RESUMEN

With innovative force plate technology being available to many sports organizations worldwide that allow for time-efficient in-depth neuromuscular performance assessment, the purpose of the present study was to examine the relationship between some of the most commonly analyzed countermovement vertical jump (CVJ) force-time metrics and basketball playing time and efficiency. Twenty-four professional male basketball players volunteered to participate in the present study. The CVJ testing procedures were conducted within the first quarter of the competitive season span. Following a standardized warm-up protocol, each athlete stepped on a dual uni-axial force plate system sampling at 1,000 Hz and performed three maximum-effort CVJs with no arm swing. To minimize the possible influence of fatigue, each jump trial was separated by a 10-15 s rest interval and the average value across three jumps was used for performance analysis purposes. Basketball playing efficiency and average playing time were obtained at the end of the regular season competitive period from the coaching staff records and the official team records. Pearson product-moment correlation coefficients (r) were used to examine the strength of the relationships between force-time metrics and basketball playing time and efficiency, separately for each dependent variable (p < 0.05). A significant positive association was observed between playing efficiency and eccentric mean force and eccentric mean and peak power (r = 0.406-0.552). Similarly, an increase in eccentric mean power was positively correlated with the number of minutes played during the competitive season (r = 0.464). Moreover, the aforementioned relationship remained present even when eccentric mean power was expressed relative to the player's body mass (r = 0.406). Thus, the findings of the present study indicate that, at the professional level of men's basketball competition, CVJ eccentric strength and power have a positive impact on both playing time and efficiency.

7.
J Strength Cond Res ; 38(7): 1326-1329, 2024 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-38900179

RESUMEN

ABSTRACT: Cabarkapa, D, Johnson, QR, Cabarkapa, DV, Philipp, NM, Eserhaut, DA, and Fry, AC. Changes in countermovement vertical jump force-time metrics during a game in professional male basketball players. J Strength Cond Res 38(7): 1326-1329, 2024-As technology within elite basketball advances and is more available to sporting organizations, novel approaches for assessing and addressing athletic performance during practice or competition are being continuously explored. The aim of this investigation was to examine changes in neuromuscular performance during live basketball play. Eight professional male basketball players volunteered to participate in this study. The testing procedures were conducted during a pre-tournament camp over a span of 2 days. During the first day, the athletes were familiarized with the testing procedures, and baseline measurements were obtained. Using a uni-axial force plate system sampling at 1,000 Hz, each athlete performed 3 countermovement vertical jumps (CVJ) without an arm swing before proceeding with their regular training activities. During the second day of the pre-tournament camp, the athletes repeated identical CVJ testing procedures before the start of the first quarter and post-first, second, third, and fourth quarter of a simulated 5-on-5 basketball game. Repeated-measures testing design was used to examine statistically significant differences in various force-time metrics of interest in comparison to the baseline levels (p < 0.05). Besides a trivial decrease in eccentric mean force, the findings of this study revealed no statistically significant changes in any force-time metrics of interest within both eccentric and concentric phases of the CVJ (i.e., mean and peak force and power, jump height, impulse, velocity, and contraction time). Thus, we can conclude that these variables were not sensitive to acute fatigue, suggesting that the neuromuscular performances of professional male basketball players tend to remain unchanged throughout a 5-on-5 simulated game.


Asunto(s)
Rendimiento Atlético , Baloncesto , Humanos , Baloncesto/fisiología , Masculino , Rendimiento Atlético/fisiología , Adulto Joven , Fuerza Muscular/fisiología , Adulto , Movimiento/fisiología , Atletas , Músculo Esquelético/fisiología , Fenómenos Biomecánicos , Prueba de Esfuerzo/métodos
8.
Front Sports Act Living ; 6: 1387918, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38840953

RESUMEN

The purpose of the present study was to examine differences in game-related statistics between winning and losing game outcomes and determine which performance parameters have the greatest impact in classifying winning from losing game outcomes at the National Collegiate Athletic Association (NCAA) Division-I men's basketball level of competition. The data scraping technique was used to obtain publicly available data over a 2018-2019 season span. The total number of games examined was 5,147. Independent t-tests were used to examine statistically significant differences between winning and losing game outcomes, while a full model discriminant function analysis was used to determine the relative contribution of each game-related statistic and its ability to classify winning from losing game outcomes (p < 0.05). Alongside scoring a greater number of points at the end of the game, the findings of the present study indicate that winning teams: (a) attempted and made more field goals, three-point, and free-throw shots, (b) accumulated more defensive and total rebounds, assists, steals, and blocks, (c) had fewer turnovers and personal fouls, and (d) secured greater field goal, three-point, and free-throw shooting percentage. Moreover, the top three performance parameters discriminating winning from losing game outcomes were field goal percentage, defensive rebounds, and assists, accounting for 16.8%, 12.2%, and 12.0% of the total percentage of explained variance, respectively (i.e., 41.0% combined). Overall, these findings support the expected roles of offensive and defensive game-related statistics and provide further insight into how they work together to optimize the chances of securing the desired game outcome.

9.
J Biol Chem ; 300(6): 107409, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38796063

RESUMEN

About 18% of all human cancers carry a mutation in the KRAS gene making it among the most sought-after anticancer targets. However, mutant KRas protein has proved remarkably undruggable. The recent approval of the first generation of RAS inhibitors therefore marks a seminal milestone in the history of cancer research. It also raises the predictable challenges of limited drug efficacies and acquired resistance. Hence, new approaches that improve our understanding of the tumorigenic mechanisms of oncogenic RAS within more physiological settings continue to be essential. Here, we have used the near-diploid hTERT RPE-1 cells to generate isogenic cell lines in which one of the endogenous KRAS alleles carries an oncogenic KRAS mutation at glycine 12. Cells with a KRASG12V/+, KRASG12C/+, or KRASG12D/+ genotype, together with WT KRASG12G(WT)/+ cells, reveal that oncogenic KRAS.G12X mutations increase cell proliferation rate and cell motility and reduced focal adhesions in KRASG12V/+ cells. Epidermal growth factor -induced phosphorylation of ERK and AKT was comparable between KRASG12V/+, KRASG12C/+, KRASG12D/+, and KRASG12G(WT)/+ cells. Interestingly, KRASG12X/+ cells showed varying responses to distinct inhibitors with the KRASG12V/+ and KRASG12D/+ cells more sensitive to hydroxyurea and MEK inhibitors, U0126 and trametinib, but more resistant to PI3K inhibitor, PIK-90, than the KRASG12G(WT)/+ cells. A combination of low doses of hydroxyurea and U0126 showed an additive inhibition on growth rate that was greater in KRASG12V/+ than WT cells. Collectively, these cell lines will be a valuable resource for studying oncogenic RAS signaling and developing effective anti-KRAS reagents with minimum cytotoxicity on WT cells.


Asunto(s)
Movimiento Celular , Proliferación Celular , Proteínas Proto-Oncogénicas p21(ras) , Humanos , Movimiento Celular/efectos de los fármacos , Proteínas Proto-Oncogénicas p21(ras)/genética , Proteínas Proto-Oncogénicas p21(ras)/metabolismo , Proliferación Celular/efectos de los fármacos , Telomerasa/genética , Telomerasa/metabolismo , Proteínas ras/metabolismo , Proteínas ras/genética , Pirimidinonas/farmacología , Piridonas/farmacología , Mutación Missense , Línea Celular , Proteínas Proto-Oncogénicas/metabolismo , Proteínas Proto-Oncogénicas/genética , Nitrilos/farmacología , Proteínas Proto-Oncogénicas c-akt/metabolismo , Proteínas Proto-Oncogénicas c-akt/genética , Butadienos/farmacología , Sustitución de Aminoácidos , Mutación
10.
Hum Mol Genet ; 33(17): 1506-1523, 2024 Aug 18.
Artículo en Inglés | MEDLINE | ID: mdl-38776958

RESUMEN

The ubiquitin-proteasome system mediates the degradation of a wide variety of proteins. Proteasome dysfunction is associated with neurodegenerative diseases and neurodevelopmental disorders in humans. Here we identified mutations in PSMC5, an AAA ATPase subunit of the proteasome 19S regulatory particle, in individuals with neurodevelopmental disorders, which were initially considered as variants of unknown significance. We have now found heterozygotes with the following mutations: P320R (6 individuals), R325W, Q160A, and one nonsense mutation at Q69. We focused on understanding the functional consequence of PSMC5 insufficiency and the P320R mutation in cells and found that both impair proteasome function and activate apoptosis. Interestingly, the P320R mutation impairs proteasome function by weakening the association between the 19S regulatory particle and the 20S core particle. Our study supports that proteasome dysfunction is the pathogenic cause of neurodevelopmental disorders in individuals carrying PSMC5 variants.


Asunto(s)
Mutación , Trastornos del Neurodesarrollo , Complejo de la Endopetidasa Proteasomal , Complejo de la Endopetidasa Proteasomal/genética , Complejo de la Endopetidasa Proteasomal/metabolismo , Humanos , Trastornos del Neurodesarrollo/genética , Trastornos del Neurodesarrollo/patología , Apoptosis/genética , Masculino , Femenino , Ubiquitina/metabolismo , Ubiquitina/genética , Células HEK293
11.
Front Sports Act Living ; 6: 1394739, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38799031

RESUMEN

Given its fast-growing popularity and unique on-court competitive demands, 3 × 3 basketball has captured a considerable amount of attention over recent years. However, unlike research focused on studying 5 × 5 basketball players, there is a lack of scientific literature focused on examining countermovement vertical jump (CMJ) and sprint performance characteristics of 3 × 3 athletes. Thus, the purpose of the present study was to compare force-time metrics during both eccentric and concentric phases of the CMJ and acceleration and deceleration capabilities between 3 × 3 and 5 × 5 top-tier professional male basketball athletes. Ten 3 × 3 and eleven 5 × 5 professional basketball players volunteered to participate in the present study. Upon completion of a standardized warm-up, each athlete performed three maximum-effort CMJs, followed by two 10 m sprints. A uni-axial force plate system sampling at 1,000 Hz was used to analyze CMJ force-time metrics and a radar gun sampling at 47 Hz was used to derive sprint acceleration-deceleration measures. Independent t-tests and Hedge's g were used to examine between-group statistically significant differences (p < 0.05) and effect size magnitudes. The findings of the present study reveal that 3 × 3 and 5 × 5 professional male basketball players tend to display similar neuromuscular performance characteristics as no significant differences were observed in any force-time metric during both eccentric and concentric phases of the CMJ (g = 0.061-0.468). Yet, prominent differences were found in multiple measures of sprint performance, with large effect size magnitudes (g = 1.221-1.881). Specifically, 5 × 5 basketball players displayed greater average and maximal deceleration and faster time-to-stop than their 3 × 3 counterparts. Overall, these findings provide reference values that sports practitioners can use when assessing athletes' CMJ and sprint performance capabilities as well as when developing sport-specific training regimens to mimic on-court competitive demands.

12.
Front Sports Act Living ; 6: 1407601, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38783868

RESUMEN

Given the complex nature of the handball as a game, players are required to possess a distinct set of physical and physiological attributes to attain peak performance. With the countermovement vertical jump (CVJ) being widely implemented as a non-invasive and time-efficient testing modality in sports settings, the purpose of the present study was twofold: (a) to establish a CVJ profile of professional female handball players and (b) to examine differences in force-time metrics between starters and non-starters. Forty-two professional female handball players (e.g., SuperLeague) volunteered to participate in this study. Each athlete performed three maximum-effort CVJs with no arm swing while standing on a uni-axial force plate system sampling at 1,000 Hz. Independent t-tests were used to examine differences in each variable between starters and non-starters. The results revealed that starters attained superior performance within the eccentric phase of the CVJ when compared to non-starters, particularly in terms of eccentric peak velocity (-0.957 ± 0.242 vs. -0.794 ± 0.177 m·s-1), eccentric mean power (320.0 ± 77.7 vs. 267.1 ± 75.2 W), and eccentric peak power (929.0 ± 388.1 vs. 684.4 ± 214.2 W). While not reaching the level of statistical significance, moderate-to-large effect sizes were observed for concentric impulse, peak velocity, and mean and peak force and power, all in favor of players included in the starting lineup (g = 0.439-0.655). Overall, these findings suggest that at the top-tier level of handball competition, the ability to secure a spot in a starting lineup may be possibly influenced by the athlete's eccentric performance capabilities. Thus, the development of lower-body eccentric strength and power may positively impact on-court athlete performance and ultimately help the team secure the desired game outcome.

13.
Front Sports Act Living ; 6: 1377528, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38711571

RESUMEN

Introduction: While using force-plate derived measures of vertical jump performance, reflective of stretch-shortening-cycle (SSC) efficiency is common practice in sport science, there is limited evidence as to which tests and measures may be most sensitive toward neuromuscular fatigue. The aim of this study was to explore the SSC fatigue response to a one-week high-intensity fatiguing phase of training in National Collegiate Athletic Association (NCAA) Division-I basketball players. Methods: The study timeline consisted of three weeks of baseline measures, one week of high-intensity training, and two weeks of follow-up testing. Countermovement jumps (CMJ) and 10-5 hop tests were performed at baseline, as well as at two time-points during, and three time-points following the fatiguing training period, allowing for performance-comparisons with baseline. Results: Compared to the weekly training sum at baseline, during the high intensity training phase, athletes were exposed to very large increases in selected external load metrics (ES = 1.44-3.16), suggesting that athletes experienced fatigue acutely, as well as potential longer lasting reductions in performance. Vertical jump data suggested that in the CMJ, traditional metrics such as jump height, as well as metrics reflecting kinetic outputs and movement strategies, were sensitive to the stark increase in high-intensity training exposure. The 10-5 hop test suggested a fatigue-induced loss of tolerance to ground impact reflected by performance reductions in metrics related to jump height and reactive strength qualities. Discussion: These findings emphasize that when monitoring neuromuscular fatigue, variables and assessments may not be looked at individually, but rather as part of a more global monitoring approach.

14.
Exp Physiol ; 109(6): 939-955, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38643471

RESUMEN

Exercise-induced muscle adaptations vary based on exercise modality and intensity. We constructed a signalling network model from 87 published studies of human or rodent skeletal muscle cell responses to endurance or resistance exercise in vivo or simulated exercise in vitro. The network comprises 259 signalling interactions between 120 nodes, representing eight membrane receptors and eight canonical signalling pathways regulating 14 transcriptional regulators, 28 target genes and 12 exercise-induced phenotypes. Using this network, we formulated a logic-based ordinary differential equation model predicting time-dependent molecular and phenotypic alterations following acute endurance and resistance exercises. Compared with nine independent studies, the model accurately predicted 18/21 (85%) acute responses to resistance exercise and 12/16 (75%) acute responses to endurance exercise. Detailed sensitivity analysis of differential phenotypic responses to resistance and endurance training showed that, in the model, exercise regulates cell growth and protein synthesis primarily by signalling via mechanistic target of rapamycin, which is activated by Akt and inhibited in endurance exercise by AMP-activated protein kinase. Endurance exercise preferentially activates inflammation via reactive oxygen species and nuclear factor κB signalling. Furthermore, the expected preferential activation of mitochondrial biogenesis by endurance exercise was counterbalanced in the model by protein kinase C in response to resistance training. This model provides a new tool for investigating cross-talk between skeletal muscle signalling pathways activated by endurance and resistance exercise, and the mechanisms of interactions such as the interference effects of endurance training on resistance exercise outcomes.


Asunto(s)
Músculo Esquelético , Resistencia Física , Entrenamiento de Fuerza , Transducción de Señal , Humanos , Transducción de Señal/fisiología , Músculo Esquelético/fisiología , Músculo Esquelético/metabolismo , Entrenamiento de Fuerza/métodos , Resistencia Física/fisiología , Animales , Adaptación Fisiológica/fisiología , Ejercicio Físico/fisiología , Modelos Biológicos
15.
Front Sports Act Living ; 6: 1389001, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38590296

RESUMEN

As one of the fundamental volleyball skills, countermovement vertical jump (CMJ) has been commonly implemented in the applied sports setting as a non-invasive and time-efficient assessment of athletes' lower-body neuromuscular function. The purpose of the present study was to examine the differences in CMJ characteristics between starters and non-starters within a cohort of professional female volleyball players. Nineteen athletes competing in one of the top European leagues (i.e., SuperLeague) volunteered to participate in the present investigation. Following the completion of a warm-up protocol, each athlete performed three maximal-effort CMJs with no arm swing while standing on a uni-axial force plate system sampling at 1,000 Hz. The following force-time metrics were used for performance analysis purposes: braking phase duration and impulse, eccentric and concentric duration, mean and peak force and power, contraction time, jump height, and reactive strength index-modified. Mann-Whitney U and independent t-tests revealed no statistically significant differences (p > 0.05) during both eccentric and concentric phases of CMJ between the players included in the starting lineup (n = 9) and their substitutions (n = 10), with the effect sizes being small to moderate in magnitude (g = 0.053-0.683). While further research is warranted on this topic, these results suggest that securing a position in a starting lineup at the professional level of volleyball play may be more contingent on the player's ability to proficiently execute sport-specific skills (e.g., blocking, attacking), rather than the performance on the CMJ assessment, considering that the observed values for both groups fall within the desired ranges for this specific population of athletes.

16.
J Biol Chem ; 300(5): 107144, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38458397

RESUMEN

Echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) oncogenic fusion proteins are found in approximately 5% of non-small cell lung cancers. Different EML4-ALK fusion variants exist with variant 3 (V3) being associated with a significantly higher risk than other common variants, such as variant 1 (V1). Patients with V3 respond less well to targeted ALK inhibitors, have accelerated rates of metastasis, and have poorer overall survival. A pathway has been described downstream of EML4-ALK V3 that is independent of ALK catalytic activity but dependent on the NEK9 and NEK7 kinases. It has been proposed that assembly of an EML4-ALK V3-NEK9-NEK7 complex on microtubules leads to cells developing a mesenchymal-like morphology and exhibiting enhanced migration. However, downstream targets of this complex remain unknown. Here, we show that the microtubule-based kinesin, Eg5, is recruited to interphase microtubules in cells expressing EML4-ALK V3, whereas chemical inhibition of Eg5 reverses the mesenchymal morphology of cells. Furthermore, we show that depletion of NEK7 interferes with Eg5 recruitment to microtubules in cells expressing EML4-ALK V3 and cell length is reduced, but this is reversed by coexpression of a phosphomimetic mutant of Eg5, in a site, S1033, phosphorylated by NEK7. Intriguingly, we also found that expression of Eg5-S1033D led to cells expressing EML4-ALK V1 adopting a more mesenchymal-like morphology. Together, we propose that Eg5 acts as a substrate of NEK7 in cells expressing EML4-ALK V3 and Eg5 phosphorylation promotes the mesenchymal morphology typical of these cells.


Asunto(s)
Cinesinas , Quinasas Relacionadas con NIMA , Proteínas de Fusión Oncogénica , Quinasas Relacionadas con NIMA/metabolismo , Quinasas Relacionadas con NIMA/genética , Humanos , Fosforilación , Proteínas de Fusión Oncogénica/metabolismo , Proteínas de Fusión Oncogénica/genética , Cinesinas/metabolismo , Cinesinas/genética , Microtúbulos/metabolismo , Microtúbulos/genética , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patología , Neoplasias Pulmonares/genética , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Carcinoma de Pulmón de Células no Pequeñas/patología , Carcinoma de Pulmón de Células no Pequeñas/genética , Mesodermo/metabolismo , Mesodermo/patología , Línea Celular Tumoral , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Serina-Treonina Quinasas/genética
17.
IEEE Open J Eng Med Biol ; 5: 140-147, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38445237

RESUMEN

Goal: Urinary incontinence (UI) affects a significant proportion of the population and is associated with negative physical and psychological side-effects. Microwave-based technologies may have the potential to monitor bladder volume, providing a proactive, low-cost and non-invasive tool to support individuals with UI. Methods: Studies to date on microwave bladder monitoring have been limited to highly simplified computational and experimental scenarios. In this work, we study the most realistic models to date (both male and female), which incorporate dielectrically and anatomically representative tissues of the pelvic region. Results: We examine the ability of detecting bladder fullness through both reflection and transmission-based parameters and, for the first time, study the effect of urine permittivity. As a proof-of-concept of bladder state detection, we further investigate reconstructing differential radar images of the bladder with two different volumes of urine. Conclusions: The results indicate that there is strong potential for monitoring and detecting the bladder state using microwave measurements.

18.
J Strength Cond Res ; 38(2): e72-e77, 2024 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-38258833

RESUMEN

ABSTRACT: Cabarkapa, DV, Cabarkapa, D, Philipp, NM, and Fry, AC. Competitive season-long changes in countermovement vertical jump force-time metrics in female volleyball players. J Strength Cond Res 38(2): e72-e77, 2024-Although force plates remain one of the most widely used tools for neuromuscular performance assessment in applied sports-specific settings, there is still a lack of scientific literature focused on studying changes in countermovement vertical jump (CVJ) performance in team sports such as volleyball, especially within the female athlete population. Thus, the purpose of the present study was to examine season-long neuromuscular performance changes in volleyball players. Eighteen National Association of Intercollegiate Athletics Division-I collegiate female athletes performed 3 maximal-effort CVJs while standing on a uniaxial force plate system sampling at 1,000 Hz at 5 different testing timepoints throughout a competitive season span (∼11 weeks). The testing sessions were separated 2-3 weeks apart and performed at the approximately same time of the day (12:00 hours). Repeated-measures analysis of variance revealed that both concentric and eccentric force-time metrics remain relatively unchanged throughout a regular season span (e.g., concentric peak force and power, eccentric impulse and duration). However, the eccentric metrics such as peak and mean power and peak velocity displayed a slight improvement after a brief tapering period purposely implemented before the post-season competition to optimize the athlete's recovery (∼15, 18, and 14% increase, respectively). In addition, the outcome metrics such as vertical jump height and reactive strength index-modified did not display notable fluctuations across the competitive season span. These findings can help coaches, sports scientists, and strength and conditioning practitioners to obtain a deeper insight into collegiate female athletes' force-time characteristics that may aid with developing adequate training regimens targeted toward optimizing on-court performance.


Asunto(s)
Voleibol , Humanos , Femenino , Estaciones del Año , Atletas , Posición de Pie , Deportes de Equipo
19.
J Strength Cond Res ; 38(5): e253-e263, 2024 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-38241475

RESUMEN

ABSTRACT: Philipp, NM, Cabarkapa, D, Blackburn, SD, and Fry, AC. Dose-response relationship for external workload and neuromsuclar performance over a female, collegiate, basketball season. J Strength Cond Res 38(5): e253-e263, 2024-The aim of this study was to investigate the relationship between external workload exposure and changes in countermovement jump force-time characteristics over the course of an entire basketball season, in a sample of National Collegiate Athletic Association Division I, female, basketball players. Data for 12 players were retrospectively analyzed, with external workload being quantified by means of an exponentially weighted, acute, and chronic workload, as well as an acute:chronic workload ratio derived from an inertial measurement unit-based system worn by athletes for all practices and games during the regular season. Countermovement jumps were performed on a total of 26 test days over the span of the in-season competitive period. To statistically analyze these relationships, and to account for multiple observations of the same athletes in a data set, linear mixed-effects models with athlete identity (ID) intercept as the random effect were used. Study findings suggested that associations between external workload exposure and respective force-time characteristics after controlling for the random effect of athlete ID were dependent on the specific metric or metric subgroup used, as well as the type of workload exposure (e.g., acute vs. chronic). Force-time signatures from the braking phase (e.g., average braking force) seemed to be particularly associated with higher degrees of acute workload exposure, whereas strategy-based metrics such as countermovement depth showed significant associations with chronic workload exposure. Furthermore, model results suggested the importance of analyzing neuromuscular responses to external workload on an individual basis, rather than across an entire team. Findings might help practitioners in their selection process related to metrics of interest in monitoring neuromuscular fatigue and readiness.


Asunto(s)
Rendimiento Atlético , Baloncesto , Humanos , Femenino , Baloncesto/fisiología , Rendimiento Atlético/fisiología , Adulto Joven , Estudios Retrospectivos , Atletas/estadística & datos numéricos , Carga de Trabajo , Fuerza Muscular/fisiología
20.
J Strength Cond Res ; 38(1): 74-79, 2024 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-37815260

RESUMEN

ABSTRACT: Philipp, NM, Crawford, DA, Cabarkapa, D, and Fry, AC. Strength and power thresholds to identify high and low linear sprint speed performers in collegiate American football players. J Strength Cond Res 38(1): 74-79, 2024-Lower-body strength and power are commonly measured performance qualities across a number of sports. In recent years, more attention has been given to relationships, primarily between lower-body strength and linear speed performance. While still limited, evidence is in agreement that lower-body strength positively contributes to linear speed performance. However, what is less well understood is if there comes a point in an athlete's development, at which, further working on increasing maximal strength may not fully compliment additional gains in speed performance. Within this study, authors aimed to provide practitioners with lower-body strength and power thresholds that can discriminate between slow and fast performers, within a group of collegiate American football players. The sample was further divided into a high-body and low-body weight group, and authors hypothesized that by using logistic regression, supplemented with receiver operator curve analyses, optimal cut-off points (i.e., relative lower-body strength thresholds) that are able to significantly discriminate between slow and fast linear speed performers may be identified. Findings indicate that optimal cut-off scores differed between the groups of athletes, as well as the lower body strength and power tests. All models were able to significantly distinguish between slower and faster performers, and area under the curve values ranged from 0.695 to 0.903. Although thresholds will likely vary based on factors such as sex, training age, and sport, findings from this investigation may be used to benchmark athletes and to further individualize training aimed at improving linear speed performance.


Asunto(s)
Rendimiento Atlético , Fútbol Americano , Humanos , Universidades , Atletas , Fuerza Muscular
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