Effect of bioresonance therapy on antioxidant system in lymphocytes in patients with rheumatoid arthritis.

We measured activities of superoxide dismutase, catalase, and glutathione peroxidase and content of nonprotein thiol groups (reduced glutathione) in blood lymphocytes from patients with rheumatoid arthritis before and during bioresonance therapy. The state of the antioxidant system in lymphocyte from patients receiving standard pharmacotherapy was characterized by activation of the key antioxidant enzymes and decreased content of thiol groups. Bioresonance therapy increased the content of thiol groups and normalized activities of superoxide dismutase and glutathione peroxidase. However, catalase activity remained above the control. Changes in the lymphocyte antioxidant system indicate that bioresonance therapy activates nonspecific protective mechanisms in patients with rheumatoid arthritis.

[Immunological and functional characteristics of epitopes and regions of gB glycoprotein of Aujeszky's disease virus]. / Immunologicheskaia i funktsional'naia kharakteristika épitopov i oblastei glikoproteina gB virusa bolezni Aueski.

The role of different gB epitopes and regions at some stages of virus replication in cell cultures and in the formation of immunity to Aujeszky's disease virus (ADV) was studied using a panel of 13 monoclonal antibodies (MAB) that recognize glycoprotein gB (gB) of ADV and antisera against fusion recombinant proteins expressing gB fragments. Productive infection following virion attachment was prevented by antibodies to the N-terminal domain of gB. Three MABs against the N-terminal domain of gB and 5 MABs directed against the immunodominant region located in the gBc-subunit of gB inhibited the cell-to-cell spread of viral infection. After immunization with recombinant proteins expressing the N-terminal fragments of gB 80% mice were protected from lethal ADV challenge. After passive immunization the majority of MABs protected 20-80% mice from lethal ADV challenge. Hence, the N-terminal domain of ADV gB is associated with the virus penetration into the cell and is important for anti-ADV immunity.

Structural and chemical changes in the cytoskeleton of the goldfish Mauthner cells after vestibular stimulation.

The ultrastructure and protein content of goldfish Mauthner cells (M-cells) at different functional states induced by natural vestibular stimulations were studied. 2-h stimulation, usually causing a fatiguing of the fishes, was found to be accompanied by ultrastructural changes within M-cells and a decreased content of cytoskeletal proteins. After training by short stimulations resulting in a long-term adaptation of the fishes, the ultrastructure and protein content of M-cells could not be distinguished qualitatively and quantitatively from those of non-adapted fishes. When the adapted fishes were stimulated for 2 h the content of a 70-kDa protein was found to be increased. In addition, the content of a 42-kDa protein, obviously actin, was elevated in this case. Correspondingly, electron microscopic analysis demonstrated a significantly increased resistance of the cytoskeleton to fatiguing stimulation. The data obtained indicate that the neural cytoskeleton is a central target of fatiguing stimulation. We suppose that the 70-kDa protein is responsible for the adaptive properties of the cytoskeleton. This protein is assumed to be identical with one of the so-called heat-shock proteins of non-neural cells which have the same electrophoretic mobility and are also able to protect the cytoskeleton under stress conditions.