RESUMEN
Adult schistosomes were detected in the veins or capillaries of the large intestine, mesentery, liver, and adrenal glands in eight of 13 whooper swans (Cygnus cygnus) examined in Iwate Prefecture, Japan. However, neither eggs nor severe tissue injuries were observed in any of the swans. The schistosomes were definitively identified as Allobilharzia visceralis based on the nucleotide sequences of the ribosomal internal transcribed spacer (ITS) region. Allobilharzia visceralis infections have been reported in whooper swan in Iceland and tundra swan (Cygnus columbianus) in North America. These detections suggest that A. visceralis is distributed extensively along the swan flyways because the swans are migratory birds. To the best of our knowledge, this is the first report of A. visceralis infection in Asia.
Asunto(s)
Anseriformes/parasitología , Enfermedades de las Aves/parasitología , Schistosomatidae/aislamiento & purificación , Infecciones por Trematodos/veterinaria , Glándulas Suprarrenales/parasitología , Glándulas Suprarrenales/patología , Animales , Enfermedades de las Aves/epidemiología , Capilares/parasitología , ADN Espaciador Ribosómico , Huevos , Femenino , Intestino Grueso/parasitología , Intestino Grueso/patología , Japón/epidemiología , Hígado/parasitología , Hígado/patología , Masculino , Filogenia , Schistosomatidae/clasificación , Schistosomatidae/genética , Infecciones por Trematodos/epidemiología , Infecciones por Trematodos/parasitología , Venas/parasitologíaRESUMEN
Hemoplasmas cause severe infections in mammals, but these pathogens are difficult to detect and identify at the species and subspecies level because of the need for time-consuming sequence based methods. Here, we used real-time PCR with SYBR Green I targeting of the dnaK gene followed by standard melting curve analysis to achieve rapid detection and differentiation of the Mycoplasma haemomuris subspecies 'Candidatus Mycoplasma haemomuris subsp. musculi' and 'Candidatus M. haemomuris subsp. ratti'. The melting temperatures of the PCR products, 84.63 ± 0.14 °C for 'Candidatus M. haemomuris subsp. musculi', and 80.72 ± 0.16 °C for 'Candidatus M. haemomuris subsp. ratti', provided clear differentiation between them. Murine hemoplasma DNA samples, which were used as references, were confirmed for species by an analysis of 16S rRNA sequences. The protocol described herein provides a new rapid detection and identification method suitable for use with two recognized subspecies of M. haemomuris.
Asunto(s)
Proteínas HSP70 de Choque Térmico/genética , Mycoplasma/genética , Mycoplasma/aislamiento & purificación , Desnaturalización de Ácido Nucleico , Compuestos Orgánicos/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Secuencia de Bases , Benzotiazoles , Cartilla de ADN/metabolismo , Diaminas , QuinolinasRESUMEN
To establish a method for estimating the glomerular filtration rate (GFR) in conscious monkeys, the radiographic contrast medium iodixanol and the standard agent inulin were coadministered as tracers to male cynomolgus monkeys (Macaca fascicularis) as a bolus injection; blood was collected after 60, 90 and 120 min. An equation based on a single-blood-sample method derived from Jacobsson's formula was prepared using the data from healthy and saline- and gentamicin-treated monkeys by a multisample strategy with iodixanol. The GFR using the equation with iodixanol was in agreement with that from the multisample method with inulin or iodixanol. When the GFR decreased to more than 60% of the basal reference level, serum creatinine concentrations tended to increase, whereas serum blood urea nitrogen concentrations fluctuated. The results suggest that the single-sample-blood method with iodixanol is a practical tool for estimating the monkey GFR in a toxicological research setting therefore minimizing animal sufferings.
Asunto(s)
Medios de Contraste/análisis , Creatinina/sangre , Tasa de Filtración Glomerular/fisiología , Inulina/sangre , Pruebas de Función Renal/métodos , Macaca fascicularis/fisiología , Ácidos Triyodobenzoicos/sangre , Animales , Masculino , Trazadores RadiactivosRESUMEN
We examined the localization of connexin 32 (Cx32), a component of gap junctions, in 24-month-old male B6C3F1 mice with spontaneously occurring hepatocellular altered foci or tumors. Immunohistochemically, Cx32-staining intensity in cell-to-cell membranes of altered hepatocytes was decreased in eosinophilic foci and increased in basophilic foci as compared to those in intact hepatocytes. These alterations were enhanced in adenomas and carcinomas with both eosinophilic and basophilic cytoplasm. In cell membranes facing on the sinusoidal portions, the intensities increased in all lesions. Image analyses confirmed that the spot areas of Cx32 were decreased in eosinophilic foci, but increased in basophilic foci, adenomas and carcinomas. These results demonstrate that Cx32 shows different expression in different types of hepatic lesions.
Asunto(s)
Conexinas/metabolismo , Neoplasias Hepáticas/metabolismo , Adenoma/metabolismo , Adenoma/patología , Animales , Carcinoma/metabolismo , Carcinoma/patología , Uniones Comunicantes/metabolismo , Hepatocitos/metabolismo , Hígado/metabolismo , Hígado/patología , Neoplasias Hepáticas/patología , Masculino , Ratones , Proteína beta1 de Unión ComunicanteRESUMEN
The vertical transmission of Mycoplasma (M.) wenyonii was investigated in beef cattle raised on a farm in Japan by analysing the ribonuclease P RNA (rnpB) gene sequence using PCR. Peripheral blood samples from 17 dams infected with M. wenyonii and from their neonatal calves were collected and colostrum samples were taken from cows immediately after parturition, and subsequently the blood samples of calves were monitored continuously for three months. At birth on day 0, although no rnpB gene was detected in the colostrum of any of the dams, four (23.5%) of the 17 calves born were positive. At three months after delivery, the number of positive calves decreased to three. Although horizontal transmission by blood-feeding arthropod vectors has been basically accepted as the most common route of haemoplasma infection, these findings suggest that vertical transmission is, at least in part, another most likely route of M. wenyonii infection in cattle.
RESUMEN
OBJECTIVE: To establish a simplified single-blood-sample method (SBSM) involving iodixanol to estimate glomerular filtration rate (GFR) in dogs and compare data provided by that procedure with data provided by a conventional multiple-blood-sample method (MBSM) involving inulin. ANIMALS: 26 healthy dogs and 36 dogs with naturally occurring renal disease. PROCEDURES: Dogs were used in various preliminary experiments to establish protocols for the SBSM and the MBSM of GFR estimation. To evaluate the relationship between GFRs obtained by the SBSM and the MBSM each involving iodixanol, iodixanol (40 mg of I/kg) was administered IV to 26 healthy dogs and 36 dogs with renal disease; blood sample collection was performed before and at 60, 90, and 120 minutes after the injection. To evaluate the relationship between GFRs obtained by the SBSM involving iodixanol and the MBSM involving inulin, iodixanol (40 mg of I/kg) and inulin (50 mg/kg) were coadministered IV to 22 healthy dogs and 3 dogs with renal disease, followed by blood sample collection 30, 60, 90, and 120 minutes later. Serum iodixanol and inulin concentrations were separately determined by reverse-phase high-performance liquid chromatography. RESULTS: Findings revealed a correlation (r = 0.99) between GFR estimated by the SBSM and MBSM each involving iodixanol. Likewise, GFR estimated by the SBSM involving iodixanol was correlated (r = 0.89) with that estimated by the MBSM involving inulin. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that the SBSM involving iodixanol can be applied to estimate GFR in dogs, instead of use of an MBSM.
Asunto(s)
Tasa de Filtración Glomerular/veterinaria , Enfermedades Renales/veterinaria , Ácidos Triyodobenzoicos/farmacología , Animales , Estudios de Casos y Controles , Cromatografía Líquida de Alta Presión/veterinaria , Medios de Contraste/administración & dosificación , Perros , Inulina/farmacología , Enfermedades Renales/patología , Análisis de RegresiónRESUMEN
We examined the correlation between the glomerular filtration rate (GFR) estimated from an equation based on the serum iodixanol clearance technique and International Renal Interest Society (IRIS) stages of chronic kidney disease (CKD) in cats. The equation included the injection dose, sampling time, serum concentration and estimated volume of distribution (Vd) of the isotonic, nonionic, contrast medium iodixanol as a test tracer. The percent changes in the median basal GFR values calculated from the equation in CKD cats resembled those of IRIS stages 1-3. These data validate the association between the GFR derived from the simplified equation and IRIS stages based on the serum creatinine concentration in cats with CKD. They describe the GFR ranges determined using single-sample iodixanol clearance for healthy cats and cats with various IRIS stages of CKD.
Asunto(s)
Enfermedades de los Gatos/diagnóstico , Medios de Contraste/farmacocinética , Tasa de Filtración Glomerular , Insuficiencia Renal Crónica/veterinaria , Ácidos Triyodobenzoicos/farmacocinética , Animales , Estudios de Casos y Controles , Enfermedades de los Gatos/sangre , Enfermedades de los Gatos/fisiopatología , Gatos , Creatinina/sangre , Femenino , Tasa de Filtración Glomerular/fisiología , Masculino , Insuficiencia Renal Crónica/sangre , Insuficiencia Renal Crónica/diagnóstico , Insuficiencia Renal Crónica/fisiopatología , Índice de Severidad de la Enfermedad , Ácidos Triyodobenzoicos/sangreRESUMEN
To clarify the effect of renal dysfunction on pharmacokinetics of the prokinetic agent metoclopramide (MCP), we administered intravenously 0.4 mg/kg MCP to healthy calves and calves subjected to right kidney vessel ligation (ligation) without or with a subsequent left nephrectomy (ligation plus removal). Plasma MCP concentration, glomerular filtration rate (GFR) and plasma prolactin level were measured by liquid chromatography-tandem mass spectrometry, simplified equation using iodixanol and enzyme-linked immunosorbent assay, respectively. Only in calves with ligation plus removal, plasma MCP concentrations were increased significantly 6, 8 and 12 hr after injection, showing that a negative correlation was observed between the plasma MCP concentrations and GFR value. A tendency to increase in plasma PRL concentration was noted also in these calves. In conclusions, plasma MCP concentrations depend on the GFR mode in calves, and its critical GFR value was estimated.
Asunto(s)
Antieméticos/farmacocinética , Enfermedades de los Bovinos/metabolismo , Metoclopramida/farmacocinética , Insuficiencia Renal/veterinaria , Animales , Antieméticos/sangre , Área Bajo la Curva , Bovinos , Enfermedades de los Bovinos/sangre , Tasa de Filtración Glomerular , Semivida , Ligadura , Metoclopramida/sangre , Nefrectomía , Insuficiencia Renal/etiologíaRESUMEN
To testify the relevance of Jacobsson's equation for estimating bovine glomerular filtration rate (GFR), we prepared an integrated formula based on its equation using clinically healthy dairy (n=99) and beef (n=63) cows, and cows with reduced renal function (n=15). The isotonic, nonionic, contrast medium iodixanol was utilized as a test tracer. The GFR values estimated from the integrated formula were well consistent with those from the standard multisample method in each cow strain, and the Holstein equation prepared by a single blood sample in Holstein dairy cows. The basal reference GFR value in healthy dairy cows was significantly higher than that in healthy beef cows, presumably due to a breed difference or physiological state difference. It is concluded that the validity for the application of Jacobsson's equation to estimate bovine GFR is proven and it can be used in bovine practices.
Asunto(s)
Bovinos/fisiología , Medios de Contraste , Tasa de Filtración Glomerular , Ácidos Triyodobenzoicos , Animales , Femenino , Soluciones IsotónicasRESUMEN
To develop an expedient procedure for estimating glomerular filtration rate (GFR) in conscious mice, we first established a simple technique for repeated blood collections from the tail vein using a microcapillary tube attached to a 24-gauge needle without the hub. Then, we devised a definition equation for estimation of the GFR using the contrast medium iodixanol as a test tracer. Iodixanol was administered as a bolus injection at 1500 mg I/kg to ddY mice, and the GFR was determined by the conventional multisample strategy. Based on cumulative data from the multisample method, an equation for the single-blood-sample method including the iodixanol dose, estimated distribution volume (Vd), and plasma iodixanol concentration at 60 min later was sought. The GFR values from the multisample method were in good agreement with those calculated using the equation. In clinically healthy mice, the GFR decreased gradually with ages from 11 weeks old in both sexes, suggesting the necessity of the corresponding control in each protocol. In nephropathy mice induced by cisplatin, the GFR values decreased with rises in serum BUN and creatinine concentrations, and serum creatinine became elevated when the GFR decreased to approximately 70% of the basal value. The results suggest that the simplified equation using iodixanol, allowing for the repeated use of the same mice, is a versatile procedure for research purposes.
RESUMEN
To estimate the glomerular filtration rate (GFR) in cynomolgus monkeys (Macaca fascicularis), a three-blood-sample method using iodixanol was assessed in comparison with the conventional multisample strategy using inulin. Iodixanol and inulin were coadministered intravenously 40 mg I/kg and 50 mg/kg, respectively, to male monkeys, followed by blood collection 60, 90 and 120 min later. A close correlation (r=0.96) was noted between the GFR values estimated by both methods. In clinically healthy monkeys, the basal values were determined to be 3.06 ± 0.50 ml/min/kg. This is the first report, suggesting that serum clearance of iodixanol is a ready-to-use tool for a screening the GFR in monkeys, although it is necessary to perform a more longitudinal study using animals with reduced renal function.
Asunto(s)
Tasa de Filtración Glomerular/fisiología , Macaca fascicularis/fisiología , Animales , Medios de Contraste/farmacología , Relación Dosis-Respuesta a Droga , Inulina/farmacología , Masculino , Valores de Referencia , Ácidos Triyodobenzoicos/farmacologíaRESUMEN
Cisplatin is a widely used chemotherapeutic agent, but its use is limited by nephrotoxicity associated with mitochondrial dysfunction. Because its mechanisms are poorly understood, we aimed to identify the mitochondrial proteins targeted by cisplatin. We isolated renal mitochondrial proteins from Sprague-Dawley (SD) rats and performed cisplatin-affinity column chromatography. The proteins eluted were detected on SDS-PAGE and subjected to in-gel tryptic digestion and LC-MS/MS analysis. We identified glutamate oxaloacetate transaminase (GOT) and mitochondrial malate dehydrogenase (MDH). Next, we administered cisplatin intraperitoneally to SD rats to induce nephrotoxicity and assayed the activities of the enzymes. The results indicated that cisplatin caused a severe decrease in mitochondrial GOT activity on day 1 after cisplatin administration. Three d later, we also identified a decrease in mitochondrial MDH activity. Our results indicate that cisplatin binds to mitochondrial GOT and inhibits its activity, causing mitochondrial dysfunction and subsequent nephrotoxicity.
Asunto(s)
Antineoplásicos/administración & dosificación , Aspartato Aminotransferasa Mitocondrial/metabolismo , Cisplatino/administración & dosificación , Riñón/efectos de los fármacos , Animales , Antineoplásicos/toxicidad , Aspartato Aminotransferasa Mitocondrial/antagonistas & inhibidores , Aspartato Aminotransferasa Mitocondrial/química , Cisplatino/toxicidad , Malato Deshidrogenasa/química , Malato Deshidrogenasa/metabolismo , Mitocondrias/efectos de los fármacos , Mitocondrias/enzimología , Mitocondrias/patología , Oxidación-Reducción , Ratas , Ratas Sprague-Dawley , Espectrometría de Masas en TándemRESUMEN
To compare glomerular filtration rate (GFR) estimated by a single blood sample method, the non-ionic contrast medium iodixanol (40 mg I/kg) and the standard GFR tracer inulin (50 mg/kg) were co-administered as a bolus intravenous injection to 12 cats, followed by blood collection 60 and 90 mins later. Serum iodixanol and inulin concentrations were measured separately by high-performance liquid chromatography and colourimetric assay. A correlation (r = 0.90, P <0.01) was noted between GFR values estimated by the single-blood-sample method using iodixanol and inulin, indicating that this procedure can apply to feline GFR estimates, even if different GFR tracers are used. In a feline kidney transplantation study, the GFR was monitored subsequently by this simplified iodixanol method throughout a 750-day observation period with no adverse reactions. The results demonstrate that the simplified method, including the volume of distribution, can be used as an alternative or expedient tool in a clinically relevant situation.
Asunto(s)
Enfermedades de los Gatos/sangre , Tasa de Filtración Glomerular/veterinaria , Riñón/fisiología , Insuficiencia Renal Crónica/veterinaria , Animales , Nitrógeno de la Urea Sanguínea , Enfermedades de los Gatos/diagnóstico , Gatos , Creatinina , Trasplante de Riñón/veterinaria , Insuficiencia Renal Crónica/sangre , Insuficiencia Renal Crónica/diagnósticoRESUMEN
The prevalence of hemotropic mycoplasmas in wild rodents is largely unknown. Here, we report the presence of hemoplasmas in blood samples collected from brown sewer rats (Rattus norvegicus) trapped during rodent control around an animal hospital in Morioka, Japan. We examined nine rats using real-time PCR and end-point PCR, and found one rat (11.1%) that was positive for a hemoplasma infection. The 16S rRNA gene and 16S to 23S rRNA intergenic spacer region of the hemoplasma detected in a wild-caught rat were amplified using PCR. The nucleotide sequences of the PCR products were further determined and compared to those of other hemoplasmas. Our examinations revealed the presence of a hemoplasma that has not previously been described in rodents. The pathogenic traits of this hemoplasma remain unexplored.
Asunto(s)
Infecciones por Mycoplasma/veterinaria , Mycoplasma , Ratas , Enfermedades de los Roedores/epidemiología , Enfermedades de los Roedores/microbiología , Animales , Secuencia de Bases , Cartilla de ADN/genética , ADN Espaciador Ribosómico/genética , Japón/epidemiología , Datos de Secuencia Molecular , Infecciones por Mycoplasma/epidemiología , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , ARN Ribosómico 16S/genética , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria , Alineación de Secuencia , Análisis de Secuencia de ADN/veterinaria , Especificidad de la EspecieRESUMEN
Connexin 32 (Cx32) is a major gap junction protein in the liver. Neoplastic and non-neoplastic lesions were examined in Cx32-deficient (Cx32KO) mice maintained for 24-month, and compared with those in wild-type mice as a corresponding control. In neoplastic lesions, hepatocellular carcinoma increased significantly only in male Cx32KO mice, suggesting that Cx32 deficiency may be related to their pathogenesis. For females, the incidence of pituitary adenoma in the pars distalis of Cx32KO mice was lower than that of wild-type mice. No non-neoplastic lesions related to Cx32-deficiency were observed in the Cx32KO mice. In conclusion, these results demonstrate that the incidence of hepatocellular carcinoma increases only in male Cx32KO mice, presumably due to enhanced tumor promotion and progression signals associated with Cx32 deficiency.
Asunto(s)
Adenoma/patología , Carcinoma Hepatocelular/patología , Conexinas/metabolismo , Regulación de la Expresión Génica/fisiología , Neoplasias Hepáticas/patología , Neoplasias Hipofisarias/patología , Adenoma/genética , Adenoma/metabolismo , Animales , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/metabolismo , Conexinas/genética , Femenino , Predisposición Genética a la Enfermedad , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , Masculino , Ratones , Ratones Noqueados , Neoplasias Hipofisarias/genética , Neoplasias Hipofisarias/metabolismo , Factores Sexuales , Proteína beta1 de Unión ComunicanteRESUMEN
We utilized autoradiography to visualize radioactive contamination in the skeletal muscles of a pig raised within 20 km of the Fukushima Daiichi nuclear power plant following the nuclear accident. The autoradiogram of control muscle showed relatively homogenous exposure. In contrast, the autoradiogram of affected muscle showed a heterogeneous and sporadically dense imaging pattern. Photo luminescence densities of the affected and control muscles were 3.89 ± 0.67 and 2.13 ± 0.43 PSL/mm(2), respectively. This difference indicated that radioactive cesium was distributed in the skeletal muscle of the affected pig.
Asunto(s)
Radioisótopos de Cesio/análisis , Accidente Nuclear de Fukushima , Músculo Esquelético/química , Músculo Esquelético/diagnóstico por imagen , Porcinos/anatomía & histología , Animales , Autorradiografía/veterinaria , Japón , Mediciones Luminiscentes/veterinaria , Masculino , Radioisótopos de Potasio/análisis , RadiografíaRESUMEN
A quantitative assay method using LC/ESI-MS/MS for simultaneous determination of MCP in cattle plasma was developed and validated. Chromatographic separation was carried out using a multimode column (2 × 150 mm, 3 µm) with gradient elution (0.05% formic acid/methanol with 0.05% formic acid). MCP and levosulpiride (internal standard) were analyzed in the precursor/product ion pair of m/z 300.1/226.9 and 342.0/112.0, respectively. Linear calibration curves were obtained in the range of 2.5-500 ng/ml (R(2)>0.999) with a lower limit of quantification of 0.05 ng/ml. Mean recoveries were 96-103%, and the coefficient of variation was less than 6.5%. Plasma MCP concentrations after intravenous administration at 0.4 mg/kg to 12 cattle were determined by the LC-MS/MS method.
Asunto(s)
Bovinos/sangre , Cromatografía Liquida/métodos , Antagonistas de Dopamina/sangre , Metoclopramida/sangre , Espectrometría de Masas en Tándem/métodos , Animales , Bovinos/metabolismo , Antagonistas de Dopamina/farmacocinética , Límite de Detección , Metoclopramida/administración & dosificación , Metoclopramida/farmacocinética , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
The responses of 64 Holstein cows with milk fever to first treatment with 500 ml of either of 2 intravenous calcium (Ca) solutions, one containing Ca alone (group A, n = 32) or 1 containing Ca, phosphate and magnesium (group B, n = 32), were evaluated by selected clinical signs and serum biochemical data. Based on the cow's ability to stand, treatment response was categorized into "immediate response" (stood after single treatment), "delayed response" (stood after repeated treatments) and "non-response" (slaughtered despite repeated treatments). No significant differences among the response categories were found between the two groups, suggesting that the solution containing Ca borogluconate alone was sufficient for the first treatment of milk fever.
Asunto(s)
Calcio/uso terapéutico , Enfermedades de los Bovinos/tratamiento farmacológico , Parálisis de la Parturienta/tratamiento farmacológico , Animales , Calcio/administración & dosificación , Bovinos , Esquema de Medicación , Quimioterapia Combinada , Femenino , Magnesio/administración & dosificación , Magnesio/uso terapéutico , Fosfatos/administración & dosificación , Fosfatos/uso terapéutico , Embarazo , Estudios RetrospectivosRESUMEN
OBJECTIVE: To compare the use of a single-sample method involving IV administration of iodixanol with a multisample method involving inulin for the estimation of glomerular filtration rate (GFR) in cats. ANIMALS: 24 cats, including 15 healthy cats and 9 cats with naturally occurring renal diseases. PROCEDURES: Each cat was coadministered iodixanol (a nonionic contrast medium; dose providing 40 mg of I/kg) and inulin (50 mg/kg), IV, and blood samples were collected 60, 90, and 120 minutes later. Serum iodixanol and inulin concentrations were determined by means of high-performance liquid chromatography and colorimetry, respectively. Serum urea nitrogen and creatinine concentrations were also measured. RESULTS: Analysis of the data from healthy cats and cats with naturally occurring renal diseases revealed an excellent correlation between GFR values estimated by the multisample and single-sample methods with iodixanol. Likewise, GFR values estimated from the single-sample method with iodixanol were closely correlated with those calculated from the multisample method with inulin. CONCLUSIONS AND CLINICAL RELEVANCE: For estimation of GFR in cats, use of a single-sample method with iodixanol, instead of a multisample procedure, may be an expedient tool in both clinical and research settings because of its benefits to patient well-being as a result of reduced stress associated with blood sample collection.
Asunto(s)
Enfermedades de los Gatos/fisiopatología , Medios de Contraste , Tasa de Filtración Glomerular/veterinaria , Enfermedades Renales/veterinaria , Ácidos Triyodobenzoicos , Administración Intravenosa/veterinaria , Animales , Nitrógeno de la Urea Sanguínea , Enfermedades de los Gatos/sangre , Enfermedades de los Gatos/diagnóstico , Gatos , Cromatografía Líquida de Alta Presión/veterinaria , Medios de Contraste/análisis , Creatinina/sangre , Femenino , Tasa de Filtración Glomerular/fisiología , Enfermedades Renales/sangre , Enfermedades Renales/diagnóstico , Enfermedades Renales/fisiopatología , Masculino , Análisis de Regresión , Ácidos Triyodobenzoicos/sangreRESUMEN
Soil samples from the ground surface and feces and blood from a mixed-breed male pig were collected on April 10, 2011 at a farm within 20 km of the Fukushima Daiichi nuclear power plant. The radioactivity of each sample was measured using a Ge semiconductor detector. Despite the fact that the pig had been fed non-contaminated imported feed, (131)I, (134)Cs and (137)Cs were detected in the feces, and (134)Cs and (137)Cs were detected in the blood clots. Because it is considerably difficult to measure radioactive contamination in the edible muscle of living livestock, bioassays are an option for the screening of radioactive contamination in living livestock to ensure food safety.