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Front Immunol
; 9: 2044, 2018.
Article
En
| MEDLINE
| ID: mdl-30271404
Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.
B-Lymphocytes/immunology , Chemokine CCL3/metabolism , Germinal Center/immunology , Lymph Nodes/immunology , T-Lymphocytes, Regulatory/immunology , Adoptive Transfer , Animals , Cell Differentiation , Cells, Cultured , Chemokine CCL3/genetics , Chemotaxis , Immunity, Humoral , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Mice, Knockout