The importance of promoting oral health in schools: a pilot study.

Promotion of oral health has an essential role in the maintenance of teeth in adults. However, health education must start from an early age, in order to monitor the child's growth and prevent pathologies. Schools are in charge of the overall education of children and of guidance to parents but could also be engaged in the promotion of oral health, supported and counseled by pediatricians and dentists. The purpose of this pilot study is to evaluate whether school age children could be taught, successfully, basic oral sciences and dental hygiene, by a professional, during school hours. In this pilot study, an anonymized test was administered to 45 children of age between 8 and 10, both before and after an interactive lecture on oral health, to assess the effectiveness of the lesson and acquisition by the children of knowledge on oral health. After the presentation, the majority of the children were able to answer correctly to the questionnaire that was given to them (test, retest) which was related to dental anatomy and pathology (number of teeth, cavities, halitosis), and dental hygiene tools and practices (brushes, floss, mouth wash, tongue scrapers). The children seemed to be receptive to learning while in school, and a specific educational session of dental hygiene and oral health seems to be the right approach to ensure children can identify dental hygiene tools and use them appropriately.

FT-IR analysis of the Interface between Universal Scotchbond and Oral Mucosa: a preliminary in-vitro study.

BACKGROUND: The long-term success of implant therapy depends not only on proper osseointegration, but also on the healing of the epithelium and the quality of the biological seal on the abutment and on the implant neck. This study aims to evaluate the possible use of dentinal adhesives on the surface of the transmucosal path of dental implants in order to create a hermetic seal between keratinized epithelium and abutment. METHODS: Four sections of 12 µm thickness were obtained from a sample of the oral mucosa. Scotchbond TM Universal Adhesive (3M ESPE, Seefeld, Germany) was carefully applied both to the samples and to the transmucosal path of titanium abutment (Win-Six, BioSAFin, Italy). The adhesives were polymerized. FT-IR analysis was performed on: 1) polymerized Scotchbond Universal Adhesive (3M ESPE, Seefeld, Germany); 2) the interface between the titanium abutment and the adhesive; 3) the interface between the adhesive and the mucosa; 4) the mucosa samples. RESULTS: Comparing the spectra, it emerged that the adhesive has established chemical bonds both on titanium and on the keratinized mucosa, involving different types of chemical interactions. CONCLUSIONS: The results of this in-vitro study are encouraging. In the future biocompatibility and comparative study with other adhesives will be required.

Epithelial Biological Response to Machined Titanium vs. PVD Zirconium-Coated Titanium: An In Vitro Study.

The aim of this study was to compare the epithelial biological response to machined titanium Ti-6Al-4V grade 5 and titanium Ti-6Al-4V grade 5 coated with zirconia (ZrN) by physical vapor deposition (PVD). Human keratinocytes were cultured in six-well plates. Machined titanium TiAl4V4 grade 5 (T1) and ZrN-coated titanium TiAl4V4 grade 5 (T2) discs were placed in two different wells. The remaining two wells served as control (C). Scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) were performed to compare the T1 and T2 surfaces. Subsequent analyses were performed to explore the effect of T1 and T2 contact with human keratinocyte HUKE cell lines. Cell viability was evaluated using a trypan blue exclusion test and MTT assay. Cell lysates from C, T1, and T2 were Western blotted to evaluate E-cadherin and Integrin-α6ß4 expression. SEM revealed that T2 was smoother and more homogeneous than T1. EDS showed homogeneous and uniform distribution of ZrN coating on T2. Cell viability analyses did not show significant differences between T1 and T2. Furthermore, E-cadherin and Integrin-α6ß4 expressions of the epithelial cells cultured in T1 and T2 were similar. Therefore, titanium Ti-6Al-4V grade 5 surfaces coated with ZrN by PVD seem to be similar substrates to the uncoated surfaces for keratinocyte adhesion and proliferation.

Elastodontic Devices in Orthodontics: An In-Vitro Study on Mechanical Deformation under Loading.

The purpose of the present study was to evaluate the mechanical resistance of elastodontic devices (ED): their maximum compression loads and plastic deformation under loading (percentage). An Instron universal machine (Model 3365, Instron, Industrial Product Group, Grove City, PA, USA) was employed with a 100 N load cell and with Bluehill software for loading analyses. Each device was submitted to a five-cycles test. The following ED were evaluated: A.M.C.O.P. (Micerium, Genova, Italy) in red color, in orange color, and in blue color; HealthyStart (Ortho-Tain, Winnetka, IL, USA), and T4K™ phase 1 (Myofunctional Research Co., Helensvale, Australia). During the five-cycles test, the Ortho-Tain device delivered the greatest compression load (7.56 N), with the lowest percentage of deformation (0.95%). For all devices, a slight plastic deformation of the material was registered, ranging from 0.95% to 1.75%. For the T4K device it was not possible to complete the five-cycles test. For all the analyzed ED, a slight plastic deformation under loading was registered, that in all cases can be considered clinically acceptable. Further studies are needed to test the appliances after clinical usage.

Is Dental Amalgam a Higher Risk Factor rather than Resin-Based Restorations for Systemic Conditions? A Systematic Review.

OBJECTIVE: The aim of this study was to confirm the hypothesis that patients with one or more amalgam restorations have an increased risk for systemic diseases rather than patients with resin-based restorations. DATA: The data search produced an initial 3568 total number of records. All titles and abstract were reviewed by five independent examiners, and only 36 records were selected for full text in depth examination. Out of these, only nine publications matched the inclusion criteria and were included in this systematic review. SOURCES: Electronic databases (MEDLINE, Scopus, Embase, and Web of Knowledge) were searched up to June 2019. In addition, a manual search was carried out on journals related to this topic. STUDY SELECTION: All selected human clinical studies compared patients with dental amalgam restorations to patients with non-amalgam restorations on restorative material related diseases/health conditions with at least 50 patients and a reasonable follow up. The systemic effects of dental restorations were analyzed. As for any systemic effects, there was no difference between amalgam and composite restoration. CONCLUSIONS: With the limitations of the few available randomized controlled trials (RCTs) on the matter, amalgam restorations, similarly to other modern resin-based materials, were not related to an increased risk of systemic diseases or conditions. CLINICAL SIGNIFICANCE: On the basis of the available RCTs, amalgam restorations, if compared with resin-based fillings, do not show an increased risk for systemic diseases. There is still insufficient evidence to exclude or demonstrate any direct influence on general health. The removal of old amalgam restorations and their substitution with more modern adhesive restorations should be performed only when clinically necessary and not just for material concerns. In order to better evaluate the safety of dental amalgam compared to other more modern restorative materials, further RCTs that consider important parameters such as long and uniform follow up periods, number of restorations per patient, and sample populations representative of chronic or degenerative diseases are needed.

Shaping Ability of Reciproc R25 File and Mtwo System Used in Continuous and Reciprocating Motion.

AIM: Nickel-titanium (NiTi) instruments were designed to be used in continuous rotation mode with controlled speed and torque and a sequence of different sizes. The reciprocating motion was purposed to improve cyclic fatigue of rotary instruments if compared to the conventional rotation. The purpose of this work was to compare the shaping ability of Reciproc R25, Mtwo #25/0.06 used as a single file, and Mtwo sequence used in reciprocating motion and in continuous rotation. MATERIALS AND METHODS: Forty-eight endodontic training resin blocks ISO 15, 2% taper, 7 mm radius, and a 60° angle of curvature were shaped with four different protocols. Group I (Rrsf) was shaped with Reciproc R25 used as a single file in a reciprocating motion. Group II (Mrsf) was shaped with Mtwo #25/0.06 used as a single file in a reciprocating motion. Group III (MSrec) was shaped with Mtwo sequence in reciprocating motion, and finally, group IV (MSrot) was shaped with the Mtwo sequence used in continuous rotation. Preoperative and postoperative images of the simulated canals were taken under standardized conditions and combined exactly. The amount of resin removed was determined at both the inner and outer sides of the canal curvature. The ability of the instruments to remain centered in the canal was determined by calculating a centering ratio. These data were analyzed statistically using two factors analysis of variance (ANOVA) with Bonferroni correction (Bonferroni post-hoc test). RESULTS: Group Rrsf produced a greater enlargement of the canal, especially on the outer side, in the apical and middle third (p < 0.05). Group MSrot produced a lower enlargement in the middle third (p < 0.05). Group Rrsf displayed a lower centering ratio in the apical third (p < 0.05). Group MSrot displayed a lower centering ratio in the coronal third (p < 0.05). CONCLUSION: The shaping of simulated canals using a sequence of instruments in continuous rotation resulted in a more centered preparation of the apical third. The reciprocating motion for all tested instruments produced a bigger enlargement of the canals. CLINICAL SIGNIFICANCE: Reciprocating movement results in a more pronounced canal enlargement but appears to be less respectful of the original canal curvature and produces more apical transportation than a sequence of rotary NiTi files with the same ending apical size.

CAD/CAM Customized Glass Fiber Post and Core With Digital Intraoral Impression: A Case Report.

BACKGROUND: After endodontic treatment, a proper restorative technique is necessary to ensure coronal seal and protection of residual dental structure; teeth which have lost two or more walls need to be restored with posts to increase retention and stability of final restoration. Posts can be distinguished in prefabricated and customized, which are manufactured by lost wax technique or CAD-CAM. PURPOSE: Digital dentistry has been developed to increase workflow precision and to accelerate production process; use of CAD-CAM to realize customized posts was limited to scanning plaster models obtained from traditional impressions. PATIENTS AND METHODS: In the reported case an intraoral scan was used to mill a post and core restoration on an endodontically treated inferior premolar; this operative protocol was based on previous in vitro experiments that confirmed the ability of 3shape Trios scanner to read post-space up to 9 mm in depth. RESULTS: The digital technique allows us to convert the concave surface of the root canal into the convex surface of the post, and realize an anatomical post and core that improves the biomechanics of the endodontically treated tooth reducing the possibility of root fractures. CONCLUSION: The use of an intraoral digital scanner represents an opportunity for the clinician as it speeds up the production of an anatomical post and core restorations.

The relationship of tooth shade and skin tone and its influence on the smile attractiveness.

OBJECTIVE: The purpose of this clinical study was to determine if skin tone and tooth color had the same influence on smile attractiveness. MATERIALS AND METHODS: A woman's smile photograph was digitally manipulated to create a range of images with varying colors. The skin shade was modified to create four tones (p1: light, p2: light medium, p3: medium dark, p4: dark) using the L'Oreal True Illusion shade as a guide. The tooth shade was modified in four different tones: A1, A2, A3, A4 using the VITAPAN Classical shade guide to produce 16 images. A sample of 328 participants rated each image for attractiveness by means of a visual analog scale (VAS). Comparison among groups was performed with a 2-way ANOVA adjusted for multiple comparisons with the Bonferroni test (α = .05). RESULTS: Image (p3-a1) showed the highest VAS values, while image (p1-a4) obtained the lowest, (Bonferroni Test: a1 vs a2, a3, a4 P < .001; p3 vs p1, p2, p4 P < .001). Analysis performed for age, sex, level of education, and laypeople/dentists were not statistically significant (P > .05). CONCLUSIONS: Variations in tooth and skin tone can significantly influence the perception of smile attractiveness. In the tested conditions, a brighter tooth shade significantly affected the attractiveness of the smile independently from skin tone. CLINICAL SIGNIFICANCE: Understanding patient and dentist perception of the attractiveness of a smile with the important role-played by skin tone, may help clinicians to better identify teeth shade, helping delivery of tailored prostheses and esthetic restorations.

Single tooth anesthesia versus conventional anesthesia: a cross-over study.

OBJECTIVES: The aim of the present study was to compare an electronic device, the Wand Injection System (Milestone Scientific Livingstone), with conventional anesthesia in terms of the following: pain sensation during anesthetic injection; effectiveness in achieving adequate anesthesia for a complete painless dental treatment; post-operative discomfort; and patient's anxiety toward dental treatment. MATERIALS AND METHODS: Eighty adults from 18 to 70 years were enrolled in this cross-over study. Each patient served as his/her own control being subject to two anesthesia techniques: conventional and Single Tooth Anesthesia (STA) performed with the Wand. A split-mouth design was adopted in which each tooth undergoing conservative restorative or endodontic treatment received anesthesia with both techniques at 1-week interval. Before anesthetic administration, the patients' anxiety levels were determined. Physiological parameteres were measured before, during, and after the two injection procedures, and the Visual Analogue Scale (VAS) was used to assess pain of injection, discomfort, and anesthetic efficacy. Differences in assessment of pain's injection, discomfort, anesthetic efficacy, vital parameters (heart rate, blood pressure, and oxygen saturation), and state anxiety levels were analyzed using Student's t test (p value < 0.001). RESULTS: The mean injection pain and post-operative discomfort ratings with Wand were lower than those with conventional syringe (p = 0.022 and p < 0.001, respectively). No differences were found in the assessment of anesthetic efficacy. Blood pressure and heart rate mean values were lower during the anesthesia performed with the Wand than with the conventional syringe (p < 0.001). The anxiety level was higher during the first appointment, independently from the device used for the injections. CONCLUSION: The STA technique resulted in lower pain, discomfort, and lower intensity of physiological parameters. CLINICAL RELEVANCE: Single Tooth Anesthesia could be an efficacious alternative to conventional procedures.

The retrieval of unerupted teeth in pedodontics: two case reports.

INTRODUCTION: The retrieval of unerupted teeth in pedodontics is always significant to preserve the trophism of adjacent tissues, establish the correct space, provide adequate function and maintain good esthetics for the patient. The treatment plan is based on radiographic examinations and measurements, and on an accurate clinical evaluation; it aims to achieve the best treatment possible depending on the complexity of the specific case.In the most difficult clinical cases it is very important to have an early diagnosis, which is essential to plan the treatment and achieve success. In these cases, the pediatrician is in a strategic position to give an early diagnosis through a child's medical history and by counting the child's teeth. CASE PRESENTATION: This article presents two different difficult clinical cases of impacted teeth diagnosed during pediatric age, with a radiological analysis, and successfully treated with orthodontic devices designed for these specific cases. Clinical case 1 describes a 13-year-old Italian girl; clinical case 2 describes a 9-year-old Italian girl. The use of these devices achieved the desired treatment goals. The problems associated with impacted teeth and the biomechanical interventions used for these patients are discussed. CONCLUSIONS: An early and careful diagnosis followed by an accurate treatment plan for the individual cases can lead to retrieval of the impacted teeth without affecting other anatomic structures and adjacent teeth. In these cases, the pediatrician is in a strategic position to give an early diagnosis through a child's medical history and by counting the child's teeth.

In-office bacteria test for a microbial monitoring during the conventional and self-ligating orthodontic treatment.

This study investigated the microbial level of Streptococcus mutans and Lactobacillus spp. during an orthodontic treatment, and compare the data with untreated control subjects.Sixty young adult subjects were selected (average 20.5, DS 1.62), among which 40 underwent an orthodontic treatment (20 were treated with self-ligating brackets and 20 with conventional brackets) and 20 were controls. Plaque Index, salivary flow and buffering capacity of saliva were assessed before the beginning of the orthodontic treatment. Then the microbial counts were obtained by using an in-office bacteria test.The plaque index (PI) increased over time in each group as well as salivary flow, mostly in subjects treated with self-ligating brackets, suggesting a difference between conventional and self-ligating brackets. S.mutans showed a different trend of colonization in the two treated groups, as for subjects treated with conventional brackets it showed the greater value at the early stage of treatment (T1), followed by a decrease at T2. Lactobacillus spp. showed significant increase over time in the two treated groups, respect to the control group. Linear regression analysis showed no significant predictor for the microbial count at T2.The assortment of the various species of bacteria change over time during the orthodontic treatment, and seems to show different trends, depending on the type of orthodontic device. Consequently a periodical microbial monitoring using in-office bacteria tests, seems indicated.

Embryotoxicity assays for leached components from dental restorative materials.

BACKGROUND: Currently, there are no suitable assays available to evaluate the embryotoxicity of leached components from restorative dental materials. METHODS: The effect of the medium conditioned by composites and amalgam on mouse blastocysts in vitro was tested. The materials were also subcutaneously implanted, and the effect of the medium supplemented with serum from the host blood was evaluated in the embryotoxicity assay. The embryo implantation rate in the material-transplanted mothers was also evaluated. RESULTS: The results show that while the culture in media conditioned by amalgams did not affect blastocyst development, the medium conditioned by composites caused blastocyst degeneration and apoptosis. The development of blastocysts in a medium containing serum obtained from animals after transplantation was, however, without effect. Finally, inconsistent reduction in the implantation rate in transplanted mothers was observed. CONCLUSIONS: In this study, we provide examples of in vitro and in vivo tests that may be used to evaluate embryotoxicity for dental materials. Our results show that leached components from our composite-material induced embryotoxicity in vitro, however, no toxicity was observed when subcutaneously implanted in vivo. This highlights the necessity of integrated in vitro and in vivo tests for valuable predictive estimation of embryotoxicity for complex materials.

sEMG activity of masticatory, neck, and trunk muscles during the treatment of scoliosis with functional braces. A longitudinal controlled study.

BACKGROUND: Studies on the relationship between occlusal problems and the spine are of increasing interest. In this study, we monitored the sEMG activity of masticatory, neck, and trunk muscles during the treatment of scoliosis in young patients, and compared the data with a control of untreated group. SUBJECTS AND METHODS: Twelve white Caucasian patients (nine males and three females; mean age of 8.0±1.5 years) with scoliosis and Class I occlusion (without crowding) were included in this study (study group). Fifteen healthy subjects (nine males and six females; mean age of 9.5±0.8 years) were recruited as control group. The subjects were visited before they underwent the treatment of scoliosis, as well as after 3 (T1) and 6 months (T2) of their treatment for scoliosis. The patients were instructed to wear the device during sleep and during the day, according to the protocol given by their orthopedic. RESULTS: The treated group showed statistically significant changes in the sEMG activity of masticatory, neck, and trunk muscles, both at rest and during MVC of the mandible with respect to T0. The masseter and the anterior temporalis showed a significant improvement in the asymmetry index from T0 to T2. On the other hand, subjects in the control group did not register much change. CONCLUSION: Our findings suggest that the use of a functional device for the treatment of scoliosis induces a significant reduction in the asymmetry index of the trunk muscles, as well as a significant increase in the contractility of masticatory muscles.