RESUMEN
PURPOSE: Ovarian cancer (OC) is a common malignancy, and IFN-γ, a multifunctional cytokine, is unveiled to impede the multiplication and enhance apoptosis in diverse tumor cells in previous research. Nonetheless, its function and mechanism in OC are blurred. METHODS: OC cell lines SKOV3 and OVCAR3 were dealt with different concentrations (0-40 ng/ml) of IFN-γ. CCK-8 experiment was utilized to examine cell multiplication; Flow cytometry was executed to detect apoptosis and cell cycle; Wound healing assay was utilized to detect cell migration; and Transwell experiment was implemented to examine cell invasion. qRT-PCR analysis was applied to detect STAT5, STAT3, JAK2 and JAK3 mRNA expression in OC cell lines. Western blot experiment was applied to detect the protein and phosphorylation levels of SOCS1, STAT5 and STAT3. RESULTS: IFN-γ suppressed OC cell multiplication in a concentration-dependent manner. Relative to the control group, IFN-γ restrained OC cell migration, invasion, enhanced apoptosis and prevented cell transformation from G0/G1 to S phase. Further analysis revealed that IFN-γ up-modulated SOCS1 expression and impeded STAT5 and STAT3 protein phosphorylation levels, and knockdown of SOCS1 partially counteracted the inhibitory effect of IFN-γ on STAT5 and STAT3 protein phosphorylation levels. CONCLUSION: IFN-γ represses OC progression by facilitating SOCS1 to suppress STAT3 and STAT5 protein phosphorylation.
Asunto(s)
Interferón gamma/fisiología , Janus Quinasa 2/fisiología , Neoplasias Ováricas/patología , Factor de Transcripción STAT5/fisiología , Transducción de Señal , Proteína 1 Supresora de la Señalización de Citocinas/fisiología , Progresión de la Enfermedad , Femenino , Humanos , Factor de Transcripción STAT3RESUMEN
Epigenetic silencing of the GSTP1 gene by promoter methylation has been associated with increased risk and shortened survival in patients with hepatocellular carcinoma (HCC). We therefore conducted a meta-analysis to obtain a more precise estimate of this association. By searching the Cochrane Library, CBM, EMBASE, PubMed, and the Web of Science, we tabulated and analyzed parameters from each study. Results were summarized by meta-analyses using the version 12.0 STATA software. Odds ratios (ORs) and 95% confidence intervals (95%CIs) were also calculated in this analysis. A total of 14 cohort studies (tumor samples = 607, adjacent samples = 356, benign samples = 182, normal samples = 133) were included for the following statistical analysis. Our meta-analysis results demonstrated that the frequency of GSTP1 methylation in cancer tissues was significantly higher than those in adjacent tissues, benign tissues, and normal tissues (all P < 0.05). Further subgroup analysis by country indicated that the frequency of aberrant GSTP1 promoter methylation was correlated to the development of HCC among all the included experimental subgroups (all P < 0.05). The results indicate a significant association between GSTP1 methylation and poor outcomes in HCC patients.
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Carcinoma Hepatocelular/genética , Metilación de ADN , Gutatión-S-Transferasa pi/genética , Neoplasias Hepáticas/genética , Regiones Promotoras Genéticas , Adulto , Anciano , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/mortalidad , Carcinoma Hepatocelular/patología , Estudios de Cohortes , Epigénesis Genética , Femenino , Predisposición Genética a la Enfermedad , Gutatión-S-Transferasa pi/metabolismo , Humanos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/mortalidad , Neoplasias Hepáticas/patología , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Análisis de SupervivenciaRESUMEN
Os eventos isquêmicos em cães são incomuns, porém podem estar sendo subnotificados. Avaliou-se o infarto agudo do miocárdio (IAM) clinicamente, por meio de eletrocardiografia (ECG), eletrocardiografia contínua (EC), ecocardiografia (ECO), enzima creatina quinase (CK), enzima creatina quinase fração MB (CK-MB) e anátomo-histologicamente em cães sem raça definida, e observou-se a ocorrência de arritmias após injeção intramiocárdia por EC. O IAM foi obtido após a ligadura da coronária descendente anterior. Os animais apresentaram ao ECO dilatação da câmara esquerda e aumento do índice de desempenho miocárdico. Ao ECG houve desnivelamento de ST nas derivações pré-cordiais V1 e V2. No EC observaram-se arritmias ventriculares graves e supradesnivelamento de ST. As enzimas CK e CK-MB aumentaram significativamente, sendo que os picos de CK-MB e de CK ocorreram seis horas e 12 horas, respectivamente, após o IAM. Na análise histológica constatou-se infarto da parede inferior do ventrículo esquerdo e substituição do tecido muscular por tecido fibroso. Avaliou-se a injeção intramiocárdica por EC que pode servir como via terapêutica cardíaca, não sendo observado aumento das arritmias ventriculares após a injeção no miocárdio infartado. O infarto em cães pode ser detectado pelos exames cardíacos disponíveis, e a injeção intramiocárdica é uma via terapêutica cardíaca possível.
Ischemic events in dogs are uncommon; however, this may be under-reported. The myocardial infarction was created by left anterior descending coronary ligation in healthy mongrel dogs in clinical and laboratorial exams. These dogs were evaluated clinically, electrocardiography (ECG), through ambulatory electrocardiography (AE), echocardiography (ECO), creatine kinase enzyme (CK), creatine kinase MB fraction enzyme (CK-MB) and histopathologically. Even in these animals we observed the occurrence of arrhythmia after intramyocardial injection by AE. The animals exhibited left ventricular chamber enlargement and increase in myocardial performance index at ECO. In ECG, there were deviations in ST segment in the precordial leads V1 and V2. CK and CK-MB showed high increase, CK and CK-MB peaks occurred six and 12 hours after infarction, respectively. Histopathology of the infarction in the inferior wall of the left ventricle and replacement of muscle tissue by fibrous tissue were seen. Furthermore, intramyocardial injection that may be used for therapeutic purposes was evaluated by AE, which demonstrated no increase in the ventricular arrhythmias. Therefore, myocardial infarction in dogs can be detected with the tests available and intramyocardial injection can be used as a therapeutic way.
RESUMEN
The fish family Cichlidae has a large diversity and dominates African freshwater bodies, with over 200 species reported in inland waters. Sampling for the fish Oreochromis niloticus (Linnaeus) in Opa reservoir, Nigeria, started in October 1997 and extended until February 2000. The fishing methods employed for collecting the 1 430 specimens were cast netting and gillnetting. Egg diameter varied between 2.12 mm and 2.69 mm with a mean of 2.47±0.02. Female gonadosomatic index was 1.34±0.01 (0.12-4.06, n= 637). The male gonadosomatic index was 0.39±0.02 (0.03-1.67, n= 789). In Opa reservoir, O. niloticus bred throughout the study period. The species was a maternal mouth brooder with the female fish carrying eggs and fry in the buccal cavities. The sex ratio of O. niloticus was approximately 1:1 in the reservoir. The fecundity of the species was between 73 eggs and 1 810 eggs per female with a mean fecundity of 815 eggs. Rev. Biol. Trop. 55 (2): 595-602. Epub 2007 June, 29.
Estudiamos la tilapia, Oreochromis niloticus (Linnaeus) en la reserva de Opa, Nigeria, desde octubre 1997 hasta febrero del 2000. Recolectamos 1 430 especímenes con red lanzada y red de arrastre. El diámetro de los huevos varía entre los 2.12 mm y 2.69 mm con un promedio de 2.47±0.02. El índice gonadosomático de las hembras fue de 1.34±0.01 (0.12-4.06, n= 637), y en machos fue de 0.39±0.02 (0.03-1.67, n= 789). En la reserva este pez se reprodujo durante todo el periodo de estudio. Hay cuido materno: la hembra lleva los huevos y alevines en la boca. La proporción machos/hembras fue aproximadamente 1:1. La fecundidad varía entre 73 y 1 810 huevos por hembra con un promedio de 815 huevos.
Asunto(s)
Animales , Femenino , Masculino , Cíclidos/fisiología , Fertilidad/fisiología , Reproducción/fisiología , Nigeria , Estaciones del AñoRESUMEN
Major diastolics indexes, including the mitral propagation velocity (Vp), were evaluated in 36 healthy Boxer dogs, 18 males and 18 females, aging from 1.5 to 6-years-old. The aortic root and left atrium ratio, measured in B mode, was 1.17±0.11. The peaks of velocity of waves of the left atrium filling were: systolic 31.41±6.87cm/s, diastolic 73.85±17.04cm/s and atrial reversal 28.90±8.33cm/s. The ratios of the waves of initial ventricular filling were 1.58±0.19 to mitral valve and 1.62±0.29 to tricuspid valve. From the evaluated indexes, the Vp was only weakly correlated (r=0.39) with E and A waves of mitral valve and with D, showing itself, relativity, as an independence index. Regarding to the Vp, no significant differences were seen between the gender of the animals and among the observers. The Vp decreased as the age increased, showing mean values of 99.73±16.06cm/s.
Foram avaliados os principais índices diastólicos, inclusive a velocidade de propagação mitral (Vp), em 36 cães sadios da raça Boxer, 18 de cada sexo, de idade entre um ano e meio e seis anos. A relação raiz aórtica e átrio esquerdo medida no modo B foi de 1,17±0,11. Os picos de velocidades das ondas de enchimento atrial esquerdo foram: sistólica 31,41±6,87cm/s, diastólica (D) 73,85±17,04cm/s e de reversão atrial 28,90±8,33cm/s. A relação das ondas de enchimento ventricular inicial (E) e atrial (A) foi 1,58±0,19 na valva mitral e de 1,62±0,29 na valva tricúspide. Entre os índices avaliados, a Vp só se correlacionou fracamente (r=0,39) com as ondas E e A mitrais e com a onda D, mostrando-se um índice, relativamente, independente. A Vp não apresentou diferença significativa entre os sexos nem entre examinadores. A Vp diminuiu com o aumento da idade e exibiu valor médio, neste estudo, de 99,73±16,06cm/s.