BACKGROUND: Emerging studies have shown that long noncoding RNAs (lncRNAs) predominantly function in the carcinogenesis of multiple developing human tumors. The current study aimed to investigate the underlying mechanisms of LINC00337 in lung adenocarcinoma. METHODS: We analyzed TCGA and GTEx datasets and chose LINC00337 as the research object. Cell proliferation, cell apoptosis, cell cycle, migration, and invasion were detected in the gain and loss experiments of LINC00337 both in vitro and in vivo. Moreover, RNA pull-down, luciferase reporter assays, western blotting analysis, and rescue experiments were performed to investigate the underlying molecular mechanisms of LINC00337 function. RESULTS: LINC00337 expression was remarkably upregulated in lung adenocarcinoma. In addition, LINC00337 knockdown was shown to repress cell migration, invasion, and proliferation, as well as the cell cycle, and gear up apoptosis in lung adenocarcinoma in vitro and in vivo. With respect to the mechanism, LINC00337 knockdown boosted miR-1285-3p expression and then restrained YTHDF1 expression post-transcriptionally. Crucially, both miR-1285-3p decrement and YTHDF1 overexpression successfully reversed the influence on cell proliferation, migration, invasion, and apoptosis caused by LINC00337 shRNA. CONCLUSIONS: These results suggest that LINC00337 acts as an oncogenic lncRNA, targeting miR-1285-3p and regulating YTHDF1 expression, to promote the progression of lung adenocarcinoma.
The water-mediated neutral hydrolysis mechanism of carbonyl sulfide (OCS) has been re-examined using the hybrid supramolecule/continuum models with n = 2-8 explicit water cluster at the level of MP2(fc)(CPCM)/6-311++G(d,p)//MP2(fc)(CPCM)/6-31+G(d). Present calculations indicate that the potential energy surface in water solution is different from the one in the gas-phase, and only stepwise mechanism is observed in aqueous solution, i.e., monothiocarbonic acid (H2CO2S) is formed via monothiocarbonate (OCSOH(-), MTC) and its counterion, protonated water cluster, (H2O)nH3O(+). The predicted rate-determining step (RDS) barrier for the stepwise mechanism in water solution, about 90 kJ/mol, shows good agreement with the experimental values, 83.7-96.2 kJ/mol using six- or eight-water model including two cooperative water molecules. Moreover, two reaction pathways, the nucleophilic addition of water molecule across the CâO or the CâS bond of OCS are competitive.
Sulfur Oxides/chemistry , Water/chemistry , Hydrolysis , Molecular Structure , Quantum Theory
In the title compound, C(18)H(19)NO(2), the five-membered ring of the indane fragment adopts an envelope conformation with the unsubstituted carbon atom at the flap displaced by 0.412â (3)â Å from the plane formed by the other four atoms. The nitro group forms a dihedral angle of 5.3â (2)° with the indane benzene ring while the dihedral angle between the phenyl ring and the indane benzene ring is 76.74â (9)°.
The asymmetric unit of the title compound, C(22)H(22)O(4)S·2H(2)O, contains one quarter of the organic mol-ecule and one half water mol-ecule, the site symmetries of the S atom and the water O atom being mm2 and m, respectively. The dihedral angle between the benzene rings is 76.27â (11)°. In the crystal structure, inter-molecular O-Hâ¯O hydrogen bonds link the mol-ecules into chains running parallel to the a axis.
In the mol-ecule of the title compound, C(21)H(18)O(9), the two aromatic rings are aligned at an angle of 49.7â (1)°.
In the mol-ecule of the title compound, C(25)H(20)N(2)O(3), the dihedral angles formed by adjacent benzene rings are 66.75â (8), 48.37â (8) and 71.43â (9)°. In the crystal structure, centrosymmetrically related mol-ecules are linked into dimers by inter-molecular N-Hâ¯O hydrogen bonds.
The asymmetric unit of the title compound, C(20)H(18)O(8), contains two mol-ecules with small geometric differences. The dihedral angles between the benzene rings are 62.94â (12) and 59.99â (12)°. The dihedral angles between the carboxylate groups in the 2- and 3-positions are 81.72â (13) and 65.54â (15)°, respectively. However, the dihedral angles between the carboxylate groups in the 3' and 4'-positions are 67.24â (15) and 59.98â (17)°, respectively.
In the organic molecule of the title compound, C(16)H(10)O(8)·H(2)O, the dihedral angle between the two benzene rings is 42.30â (11)°. Extensive O-Hâ¯O hydrogen bonding helps to stabilize the crystal structure.
In the title compound, C(18)H(20), the five-membered ring of the indane fragment adopts an envelope conformation, with the flap atom deviating by 0.399â (3)â Å from the plane of the remaining four atoms. The dihedral angle between the phenyl ring and the indane benzene ring is 79.58â (7)°.
In the title compound, C(18)H(19)NO(2), the five-membered ring of the indane fragment adopts an envelope conformation, with the unsubstituted C atom, acting as the flap atom, deviating by 0.412â (3)â Å from the plane through the remaining four atoms. The dihedral angle between the nitro-phenyl ring and the indane benzene ring is 72.5â (1)°. The distances from the two O atoms to the plane of the adjacent benzene ring are 0.113â (4) and 0.064â (4)â Å.
The title compound, C(16)H(8)O(3), was synthesized by the Pd-coupling reaction of phenyl-acetyl-ene with 4-bromo-phthalic anhydride. The phenyl and isobenzofurane rings are nearly coplanar, forming a dihedral angle of 6.70â (10)°. In the crystal structure, centrosymmetrically related mol-ecules are linked into dimers by C-Hâ¯O hydrogen bonds.
The title compound, C(5)H(9)O(4)PS, was synthesized by the reaction of penta-erythritol with thio-phosphoryl chloride. In the crystal structure, the three six-membered rings all adopt boat conformations. Mol-ecules form chains along the c axis via inter-molecular O-Hâ¯O hydrogen bonds.
The performance and feasibility of immobilization biological activated carbon (IBAC) were investigated to treat micro-pollutant water containing nitrobenzene. IBAC has been developed on the granular activated carbon by immobilization of selected and acclimated species of engineering bacteria to treat the micro-pollutant water containing nitrobenzene. The IBAC removal efficiencies for nitrobenzene, permanganate index, turbidity, UV, ammonia and nitrite were compared with granular activated carbon (GAC) process. Biological toxicity of influent and effluent of filter were determined. Amount of bacteria in carbon was measured when carbon filter was inoculated and circulated stably. The results showed that compared with GAC, it took short time for IABC to startup and recover to normal after impact burden. In addition, IBAC was more effective to treat micro-pollutants. In order to ensure security of drinking water, the influent nitrobenzene should be controlled below 26 microg/L. Effluent biological toxicity treated with IBAC was less than that with GAC. The performance of IBAC was much better than that of GAC. Amount of bacteria in both activated carbon filter increased first and then declined from inlet to outlet.
Charcoal/chemistry , Nitrobenzenes/chemistry , Water Pollutants, Chemical/chemistry , Water Purification/methods , Adsorption , Bioreactors/microbiology , Feasibility Studies , Genetic Engineering , Nitrobacter/genetics , Nitrobacter/metabolism
