The treatment of spinal cord injury (SCI) is often ineffective. Additionally, SCI-induced inflammation leads to secondary injury. Current anti-inflammatory hydrophilic drugs fail to reach the nerve injury site due to the blood-brain barrier. Here, we synthesized MSR405, a new lipophilic unsaturated fatty acid derivative of Radix Isatidis and investigated its therapeutic effect in SCI model rats. Furthermore, we systematically investigated its structure, toxicity, anti-inflammatory effect, and the underlying mechanism. MSR405 was injected into the abdominal cavity of the Sprague Dawley SCI model rats, and the effect on their behavioral scores and pathology was estimated to assess the status of neurological inflammation. Our data show that MSR405 treatment significantly improved the motor function of SCI rats, and markedly suppressed the associated neuroinflammation. Moreover, MSR405 could attenuate LPS-induced inflammatory response in BV2 cells (Mouse microglia cells) in vitro. Mechanistically, MSR405 inhibits proinflammatory cytokines, supporting the anti-inflammatory response. Additionally, MSR405 can significantly block the TLR4/NF-κB signaling pathway and nitric oxide production. In summary, MSR405 reduces inflammation in SCI rats through the TLR4/NF-κB signal cascade and can inhibit neuroinflammation after spinal cord injury.
We demonstrated a compact and efficient Ho:YAG slab laser intra-cavity pumped by a Tm:YLF slab laser for the first time. In the Tm:YLF laser operation, the maximum power of 32.1 W with optical-to-optical efficiency of 52.8% was obtained. In the intra-cavity pumped Ho:YAG laser operation, the output power of 12.7 W at 2122â nm was obtained. The beam quality factors M2 in the vertical and horizontal directions were 1.22 and 1.11, respectively. The RMS instability was measured to be lower than 0.1%. To the best of our knowledge, this was the maximum power for the Tm-doped laser intra-cavity pumped Ho-doped laser with near-diffraction-limited beam quality.
