RESUMEN
This study investigated the impact of residual anaerobic granular sludge (AnGS) from anaerobic digesters treating molasses wastewater on ammonium reduction in a downstream aerobic granular sludge (AGS) reactor. Two conditions were tested: raw (high AnGS concentration) and settled (low AnGS concentration) anaerobically digested molasses wastewaters were fed into the AGS reactor. With the introduction of raw wastewater, enhanced nitrite accumulation at 30 % and improved total inorganic nitrogen (TIN) removal at 11 % were observed compared to 1 % nitrite accumulation and 8 % TIN removal with the introduction of settled wastewater. However, AnGS adversely affected other aspects of reactor performance, increasing effluent solid content and decreasing soluble chemical oxygen demand removal efficiency from 20 % in the low AnGS condition to 11 % in the high AnGS condition. Despite the observed retention of AnGS in the reactor, no significant bioaugmentation effects on the microbial community of the AGS were observed. Aerobic granular sludge was consistently observed in both conditions. The study suggests that AnGS may act as a nucleus for granule formation, helping to maintain granule stability in a disturbed environment. This study offers a systematic understanding of the impact of AnGS on subsequent nitrogen removal process using AGS, aiding in the decision making in the treatment of high solid anaerobic digestate.
Asunto(s)
Reactores Biológicos , Melaza , Aguas del Alcantarillado , Eliminación de Residuos Líquidos , Aguas Residuales , Aguas del Alcantarillado/microbiología , Anaerobiosis , Eliminación de Residuos Líquidos/métodos , Nitrógeno/metabolismo , AerobiosisRESUMEN
Effluent from anammox granular sludge (AnGS) bioreactor contains microbes and microbial products. This study explored mechanisms of utilizing AnGS-effluent as biostimulant for anammox process enhancement. Compared with no AnGS-effluent supplemented control reactor, 5.0 and 1.3 times higher ammonium nitrogen and total inorganic nitrogen removal rates, respectively were obtained with continuous AnGS-effluent supplementation after 98 days' operation. Anammox bacteria from Candidatus Brocadia accounted for 0.1 % (DNA level) and 1.3 %-1.5 % (RNA level) in control reactor, and 2.9 % (DNA level) and 54.5 %-55.4 % (RNA level) in the AnGS-effluent-fed reactor. Influent microbial immigration evaluation showed that bacterial immigration via AnGS-effluent supplementation was not the main contributor to active anammox community development. Amino acids biosynthesis, B-vitamins and coenzymes metabolism related pathways were facilitated by AnGS-effluent supplementation. AnGS-effluent supplementation aided anammox metabolic activity by shaping microenvironment and microbial interactions. This study provides insights into enhancing anammox bacterial metabolism with AnGS-effluent microbial products as biostimulant.
Asunto(s)
Oxidación Anaeróbica del Amoníaco , Reactores Biológicos , Eliminación de Residuos Líquidos , Purificación del Agua/métodos , Bacterias/metabolismo , Aminoácidos/biosíntesis , Nitrógeno/análisis , Nitrógeno/metabolismo , Eliminación de Residuos Líquidos/métodosRESUMEN
This study addressed the treatment of high ammonia, low biodegradable chemical oxygen demand (bCOD) anaerobically digested molasses wastewater, utilizing an aerobic granular sludge (AGS) reactor. The AGS achieved 99 % ammonia removal regardless of the bCOD supplementation. By adding low ammonia (<60 mg/L), high bCOD raw molasses wastewater (before anaerobic digestion) as a carbon source, enhanced nitrogen removal, increasing from 10 % to 97 %, and improved sludge settleability via bio-induced calcite precipitation were observed. Functional genes prediction suggested two potential denitrification pathways, including heterotrophic denitrification by Paracoccus and Thauera, and autotrophic denitrification, specifically sulfide-oxidizing autotrophic denitrification by Thiobacillus. An increase in the relative abundance of microorganisms involved in heterotrophic denitrification was observed with the addition of high bCOD raw molasses wastewater. Consequently, incorporating raw molasses wastewater into the AGS presents a sustainable approach to achieve mixotrophic denitrification, maintain stable granular sludge and ensure stable treatment performance when treating anaerobically digested molasses wastewater.
Asunto(s)
Amoníaco , Reactores Biológicos , Desnitrificación , Melaza , Aguas del Alcantarillado , Aguas Residuales , Amoníaco/metabolismo , Aguas del Alcantarillado/microbiología , Aerobiosis , Anaerobiosis , Análisis de la Demanda Biológica de Oxígeno , Purificación del Agua/métodos , Nitrógeno/metabolismo , Biodegradación AmbientalRESUMEN
Cold stress is a limiting stress factor that limits plant distribution and development; however, polyploid plants have specific characteristics such as higher resistance to abiotic stress, especially cold stress, that allow them to overcome this challenge. The cultivated cultivar Ziziphus jujuba Mill. 'Yueguang' (YG) and its autotetraploid counterpart 'Hongguang' (HG) exhibit differential cold tolerance. However, the underlying molecular mechanism and methods to enhance their cold tolerance remain unknown. Anatomical structure and physiological analysis indicated YG had a higher wood bark ratio, and xylem ratio under cold treatment compared to HG. However, the half-lethal temperature (LT50), cortex ratio, and malondialdehyde (MDA) content were significantly decreased in YG than HG, which indicated YG was cold tolerant than HG. Transcriptome analysis showed that 2084, 1725, 2888, and 2934 differentially expressed genes (DEGs) were identified in HC vs YC, H20 vs Y20, Y20 vs YC, and H20 vs HC treatment, respectively. Meanwhile, KEGG enrichment analysis of DEGs showed that several metabolic pathways, primarily plant hormone signal transduction and the MAPK signaling pathway, were involved in the differential regulation of cold tolerance between YG and HG. Furthermore, exogenous abscisic acid (ABA) and brassinolide (BR) treatments could improve their cold tolerance through increased SOD and POD activities, decreased relative electrical conductivity, and MDA content. All of these findings suggested that plant hormone signal transduction, particularly ABA and BR, might have an important role in the regulation of differential cold tolerance between YG and HG, laying the foundation for further improving cold tolerance in jujube and examining the molecular mechanisms underlying differences in cold tolerance among different ploidy cultivars.
Asunto(s)
Respuesta al Choque por Frío , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Ziziphus , Ziziphus/genética , Ziziphus/fisiología , Ziziphus/metabolismo , Respuesta al Choque por Frío/genética , Transcriptoma/genética , Frío , Malondialdehído/metabolismoRESUMEN
Neuroinflammation is considered to be a significant mechanism contributing to depression. Several studies have reported that A1 astrocytes were highly prevalent in human neuroinflammatory and neurodegenerative diseases. However, the precise mechanism by which A1 astrocytes contribute to depression remains unclear. Clinical studies have suggested a correlation between TNF-α, an activator of A1 astrocytes, and the severity of depression. Based on these findings, we hypothesized that TNF-α might worsen depression by activating A1 astrocytes. Our previous studies indicated that Rhodomyrtone (Rho) has the potential to improve depression-like behavior in mice. However, the exact mechanism for this effect has not been fully elucidated. Importantly, it was reported that Rho alleviated skin inflammation in a mouse model of psoriasis by inhibiting the expression of TNF-α. Based on this finding, we hypothesized that rhodomyrtone may exert antidepressant effects by modulating the TNF-α pathway. However, further research is required to investigate and validate these hypotheses, shedding light on the relationships between neuroinflammation, A1 astrocytes, TNF-α, and depression. By obtaining a deeper understanding of the underlying mechanisms, these findings could lead to the development of novel antidepressant strategies that target the TNF-α pathway in the context of neuroinflammation. In vivo, based on the established chronic unpredictable mild stress (CUMS) mouse depression model, we characterized the mechanism of TNF-α and Rho during depression by using several behavioral assays, adeno-associated virus(AAV) transfection, western blotting, immunofluorescence, and other experimental methods. In vitro, we characterized the effect of Rho on inflammation in TNF-α-treated primary astrocytes. TNFR1 expression was significantly increased in the hippocampus of depression-like mice, with increased astrocytes activation and neuronal apoptosis. These processes were further enhanced with increasing levels of TNF-α in the cerebrospinal fluid of mice. However, this process was attenuated by knockdown of TNFR1 and infliximab (Inf; a TNF-α antagonist). Injection of rhodomyrtone decreased the expressions of TNFR1 and TNF-α, resulting in significant improvements in mouse depression-like behaviors and reduction of astrocyte activation. TNF-α could be involved in the pathophysiological process of depression, through mediating astrocytes activation by binding to TNFR1. By blocking this pathway, Rho may be a novel antidepressant.
RESUMEN
Isopropyl acetate (IPA) and propyl acetate (PA) are recognized as promising biofuels suitable for applications as fuel additives and biodiesel models. The H-abstraction reactions with radicals stand out as the fundamental initiating reactions in the combustion kinetic models for IPA and PA. In the present work, the kinetic calculations of IPA and PA plus HO2 and OH radicals were investigated at M06-2X/cc-pVTZ//G4, M08-HX/maug-cc-pVTZ, and CCSD(T)/jul-cc-pVTZ levels. The thermodynamic calculations were obtained based on the G4 and CBS-APNO methods. Rate coefficients were calculated using both transition state theory and canonical variational transition state theory with tunneling correction at the temperature range of 250-2000 K. The total rate constants for the IPA + OH system were fitted as follows: k = 0.4674 × T3.927 exp(2128/T) (cm3 mol-1 s-1), and for the PA + OH system, the total rate constants were determined using the following equation: k = 0.0161 × T4.373 exp(2220/T) (cm3 mol-1 s-1). The rate coefficients of IPA + OH reactions determined based on the M08-HX/maug-cc-pVTZ level effectively replicate the experimental data, while H-abstraction rate coefficients of PA + OH by the CCSD(T)/jul-cc-pVTZ method accurately reproduce the experimental data. Refining the H-abstraction rate coefficients in the kinetic mechanism of PA, as proposed by Dayma et al. [Proc. Combust. Inst. 37 (2019) 429-436], has been achieved through incorporating the present calculated data, leading to the development of a revised mechanism. The validation of the updated mechanism against jet-stirred reactor data is presented, showcasing its effective performance in predicting JSR data.
RESUMEN
Anaerobically digested sludge supernatant, characterized by its high ammonia and low biodegradable chemical oxygen demand (COD) content, has raised concerns when returned to mainstream treatment lines due to potential impacts on effluent quality. Addressing this, an aerobic granular sludge (AGS) reactor adopted nitritation/denitritation with external COD addition was utilized and achieved a considerable nitrogen treatment capacity of 4.2 kg N/m3/d, reaching over 90% removal efficiencies for both ammonia and total inorganic nitrogen. This study applied progressively increased nitrogen loading to select for a microbial community that exhibited high nitrogen oxidation and reduction rates, demonstrating peak rates of 0.5 g N/g VSS/d and 3 g N/g VSS/d, respectively. The enrichment of highly efficient microbial community was achieved along with the increased biomass density peaked at 17 g/L MLVSS, with the system retaining small-sized granular sludge at 0.5 mm. The primary ammonia oxidizing bacteria was Nitrosomonas, while Thauera was the dominated denitrifiers. Quantitative polymerase chain reaction analyses reinforced the enhanced nitrogen removal capacity based on the progressively increased abundance of nitrogen cycling functional genes. The high nitrogen treatment capacity, synergistic attributes of high specific microbial activities and the substantial biomass retention, suggest the AGS's efficacy and capacity in ammonia rich wastewater treatment.
Asunto(s)
Amoníaco , Reactores Biológicos , Nitrógeno , Aguas del Alcantarillado , Reactores Biológicos/microbiología , Aguas del Alcantarillado/microbiología , Amoníaco/metabolismo , Nitrógeno/metabolismo , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/microbiología , Aguas Residuales/química , Aerobiosis , Bacterias/metabolismo , Bacterias/genética , Contaminantes Químicos del Agua/metabolismoRESUMEN
BACKGROUND: Epilepsy is a widespread and chronic disease of the central nervous system caused by a variety of factors. Mitochondrial ferritin (FtMt) refers to ferritin located within the mitochondria that may protect neurons against oxidative stress by binding excess free iron ions in the cytoplasm. However, the potential role of FtMt in epilepsy remains unclear. We aimed to investigate whether FtMt and its related mechanisms can regulate epilepsy by modulating ferroptosis. METHODS: Three weeks after injection of adeno-associated virus (AAV) in the skull of adult male C57BL/6 mice, kainic acid (KA) was injected into the hippocampus to induce seizures. Primary hippocampal neurons were transfected with siRNA using a glutamate-mediated epilepsy model. After specific treatments, Western blot analysis, immunofluorescence, EEG recording, transmission electron microscopy, iron staining, silver staining, and Nissl staining were performed. RESULTS: At different time points after KA injection, the expression of FtMt protein in the hippocampus of mice showed varying degrees of increase. Knockdown of the FtMt gene by AAV resulted in an increase in intracellular free iron levels and a decrease in the function of iron transport-related proteins, promoting neuronal ferroptosis and exacerbating epileptic brain activity in the hippocampus of seizure mice. Additionally, increasing the expression level of FtMt protein was achieved by AAV-mediated upregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) gene in the hippocampus of seizure mice. CONCLUSIONS: In epilepsy, Nrf2 modulates ferroptosis by involving the expression of FtMt and may be a potential therapeutic mechanism of neuronal injury after epilepsy. Targeting this relevant process for treatment may be a therapeutic strategy to prevent epilepsy.
Asunto(s)
Epilepsia , Ferroptosis , Masculino , Animales , Ratones , Ratones Endogámicos C57BL , Ácido Kaínico/toxicidad , Factor 2 Relacionado con NF-E2/genética , Epilepsia/inducido químicamente , Convulsiones , Ácido Glutámico , Dependovirus , Modelos Animales de Enfermedad , Ferritinas , HomeostasisRESUMEN
Granular activated carbon (GAC), a porous carbon-based material, provides increased attachment space for functional microorganisms and enhances nitrogen removal by facilitating extracellular electron transfer in the anammox process. This study investigates the effects of GAC on the biosynthesis of microbial extracellular secretions (MESs) and explores the roles of these secretions in anammox activities. Four lab-scale reactors were operated: two downstream UASB reactors (D1 and D2) receiving effluents from the upstream UASB reactors (U1: no-GAC, U2: yes-GAC). Our results indicate that MESs were enhanced with the addition of GAC. The effluent from U2 exhibited a 59.62 % higher amino acid content than that from U1. These secretions contributed to an increase in the nitrogen loading rate (NLR) in the downstream reactors. Specifically, NLR in D1 increased from 130.5 to 142.7 g N/m3/day, and in D2, it escalated from 137.5 to 202.8 g N/m3/day, likely through acting as cross-feeding substrates or vital nutrients. D2 also showed increased anammox bacterial activity, enriched Ca. Brocadia population and hao gene abundance. Furthermore, this study revealed that D2 sludge has significantly higher extracellular polymeric substances (EPS) (48.71 mg/g VSS) and a larger average granule size (1.201 ± 0.119 mm) compared to D1 sludge. Overall, GAC-stimulated MESs may have contributed to the enhanced performance of the anammox process.
Asunto(s)
Carbón Orgánico , Aguas del Alcantarillado , Aguas del Alcantarillado/microbiología , Carbón Orgánico/metabolismo , Oxidación Anaeróbica del Amoníaco , Reactores Biológicos/microbiología , Bacterias/metabolismo , Anaerobiosis , Nitrógeno/metabolismo , Oxidación-ReducciónRESUMEN
BACKGROUND: The efficacy of current immunotherapeutic strategies for patients with glioblastoma multiforme (GBM) remains unsatisfactory. The purpose of this study was to investigate the correlation between tumor necrosis factor alpha-induced protein 2 (TNFAIP2) and immunogenic cell death (ICD) in GBM, and to examine the effect of TNFAIP2 knockdown and anti-PD-1 combination treatment in a mouse glioma model. METHODS: The CGGA and TCGA databases were used to explore the possible function of TNFAIP2 in GBM. Multiplex immunohistochemistry (mIHC) staining was performed to detect the immune infiltration of tissues. Western blot, quantitative real-time polymerase chain reaction (qRT-PCR), flow cytometry, and enzyme linked immunosorbent assay (ELISA) were utilized to detect the release of damage-associated molecular patterns (DAMPs) and the activation of the immune response. A mouse glioma model was applied to examine the induction of immune response. RESULTS: In vitro and in vivo studies demonstrated that TNFAIP2 knockdown increased the surface exposure of calreticulin (CALR), heat shock protein 70 kDa (HSP70), and heat shock protein 90 kDa (HSP90) in GBM cell lines, thereby inducing immunogenic cell death (ICD). Importantly, the study found that TNFAIP2 knockdown in combination with anti-PD-1 therapy significantly improved the overall survival of glioma in a mouse model. CONCLUSIONS: TNFAIP2 knockdown induces ICD by downregulating TNFAIP2 in GBM. In addition, TNFAIP2 knockdown sensitized glioma to anti-PD-1 therapy. Hence, targeting TNFAIP2 alone or in combination with anti-PD-1 therapy may be a potential strategy for GBM treatment through ICD.
Asunto(s)
Glioblastoma , Glioma , Animales , Ratones , Humanos , Glioblastoma/patología , Muerte Celular Inmunogénica , Glioma/patología , Línea Celular , Modelos Animales de Enfermedad , Línea Celular Tumoral , CitocinasRESUMEN
BACKGROUND: Plant organs grow in a certain direction and organ twisted growth, a rare and distinctive trait, is associated with internal structure changes and special genes. The twisted branch mutant of Chinese jujube jujube, an important fruit tree native to China and introduced to nearly 50 countries, provides new typical materials for exploration of plant twisted growth. RESULTS: In this study, the cytological characteristics and related genes of twisted branches in Chinese jujube were revealed by microscopy observation and transcriptome analysis. The unique coexistence of primary and secondary structures appeared in the twisted parts of branches, and special structures such as collateral bundle, cortical bundles, and internal phloem were formed. Ninety differentially expressed genes of 'Dongzao' and its twisted mutant were observed, in which ZjTBL43, ZjFLA11, ZjFLA12 and ZjIQD1 were selected as candidate genes. ZjTBL43 was homologous to AtTBL43 in Arabidopsis, which was involved in the synthesis and deposition of cellular secondary wall cellulose. The attbl43 mutant showed significant inflorescence stem bending growth. The transgenic lines of attbl43 with overexpression of ZjTBL43 were phenotypically normal.The branch twisted growth may be caused by mutations in ZjTBL43 in Chinese jujube. AtIQD10, AtFLA11 and AtFLA12 were homologous to ZjIQD1, ZjFLA11 and ZjFLA12, respectively. However, the phenotype of their function defect mutants was normal. CONCLUSION: In summary, these findings will provide new insights into the plant organ twisted growth and a reference for investigation of controlling mechanisms of plant growth direction.
Asunto(s)
Ziziphus , Arabidopsis , Perfilación de la Expresión Génica , Mutación , Ziziphus/genéticaRESUMEN
Anaerobic ammonium oxidation (anammox) offered an energy-efficient option for nitrogen removal from wastewater. Granular activated carbon (GAC) addition has been reported that improved biomass immobilization, but the role of GAC in anammox reactors has not been sufficiently revealed. In this study, it was observed that GAC addition in an upflow anaerobic sludge blanket (UASB) reactor led to the significantly shortened anammox enrichment time (shortened by 45 days) than the reactor without GAC addition. The nitrogen removal rate was 0.83 kg N/m3/day versus 0.76 kg N/m3/day in GAC and non-GAC reactors, respectively after 255 days' operation. Acyl-homoserine lactone (AHL) quorum sensing signal molecule C8-HSL had comparable concentrations in both anammox reactors, whereas the signal molecule C12-HSL was more pervasive in the reactor containing GAC than the reactor without GAC. Microbial analysis revealed distinct anammox development in both reactors, with Candidatus Brocadia predominant in the reactor that did not contain GAC, and Candidatus Kuenenia predominant in the reactor that contained GAC. Denitrification bacteria likely supported anammox metabolism in both reactors. The analyses of microbial functions suggested that AHL-dependent quorum sensing was enhanced with the addition of GAC, and that GAC possibly augmented the extracellular electron transfer (EET)-dependent anammox reaction.
Asunto(s)
Oxidación Anaeróbica del Amoníaco , Carbón Orgánico , Reactores Biológicos/microbiología , Aguas Residuales , Aguas del Alcantarillado/microbiología , Acil-Butirolactonas , Anaerobiosis , Oxidación-Reducción , Nitrógeno , Comunicación , DesnitrificaciónRESUMEN
The role of different ammonia concentrations (mg N/L) (of 100 (carbon to nitrogen ratio (C/N) = 12; Stage I), 200 (C/N = 6; Stage II), 400 (C/N = 3; Stage III) and 200 (C/N = 6; Stage IV)) in nitrogen metabolic pathways, microbial community, and specific microbial activity were investigated in an aerobic granular sludge reactor. Heterotrophic ammonia oxidizing bacteria (HAOB) showed higher ammonia oxidation rates (AORs) than autotrophic ammonia oxidizing bacteria (AAOB) at higher C/N conditions (Stages I and II). Paracoccus was the dominant HAOB. AAOB, with only 0.2-0.3 % in relative abundance, showed 2.7-fold higher AORs than HAOB at elevated ammonia and free ammonia (FA) concentrations with C/N at 3. Nitrosomonas and a genus in Nitrosomondaceae family were the major AAOB. This study proposed that FA inhibition on heterotrophic bacteria might be the mechanism that contributes to the development of the autotrophic ammonia oxidation pathway and enrichment of AAOB.
Asunto(s)
Microbiota , Aguas del Alcantarillado , Aguas del Alcantarillado/microbiología , Nitrógeno/metabolismo , Nitrificación , Desnitrificación , Amoníaco , Carbono , Reactores Biológicos/microbiología , Redes y Vías Metabólicas , Oxidación-ReducciónRESUMEN
Peroxynitrite (ONOO-) as an active substance, is produced during normal physiological process, which plays an important role in maintaining cell REDOX balance and cell function. Moreover, the peroxynitrite is involved in many diseases and especially can be used as a biomarker of drug-induced liver injury (DILI). Therefore, in this work, we synthesized a fluorescent probe JQ-3 for detecting ONOO-. The results showed the probe JQ-3 possessed excellent selectivity, fast response time (10 min) and low detection limit (32 nM). The probe JQ-3 is almost unaffected by pH, showing the potential application in biological systems. Moreover, the probe JQ-3 can be successfully used for the detection of exogenous and endogenous ONOO- in living cells and zebrafish. At the same time, the DILI was successfully recognized by visualizing ONOO- with JQ-3 in living cells and zebrafish. Therefore, the probe JQ-3 provides a potential tool for detecting ONOO- to understand physiological and pathology processes of disease.
Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas , Ácido Peroxinitroso , Animales , Fluorescencia , Colorantes Fluorescentes/química , Colorantes Fluorescentes/toxicidad , Pez CebraRESUMEN
In this study, we constructed and displayed a ratiometric fluorescent probe JQ-2 for detecting ONOO-. The probe JQ-2 showed a ratiometric signal for visualizing ONOO- with a rapid response and high selectivity over a panel of biological analytes. Moreover, the JQ-2 has near-infrared emission (657 nm), which provides an excellent basis for the practical application in biological systems. The probe JQ-2 possessed low cytotoxicity and excellent cell membrane permeability, which can specifically visualize the exogenous and endogenous ONOO- in vitro and vivo by emission in two channels. Meanwhile, JQ-2 can be used for diagnosing drug-induced liver injury by visualizing and monitoring the fluctuations of endogenous ONOO-. Therefore, JQ-2 provided a potential tool for precisely detecting the fluctuation of ONOO- in biological systems to understand physiological and pathological process.
Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas , Ácido Peroxinitroso , Colorantes Fluorescentes , Humanos , Imagen ÓpticaRESUMEN
Mature landfill leachate wastewater (LLW) was characterized by high ammonia, refractory chemical oxygen demand (COD) and heavy metal contents, which limits the nitrogen removal in conventional activated sludge systems. Granular sludge is known to be more resistant to toxic compounds because of its dense structure and diverse microbial community. Here, granular sludge-based reactor (GSR) was applied with nitritation/denitritation (Nit/DNit) process for effective ammonia-rich mature LLW treatment at 20 °C. After a short startup period, the efficiencies of ammonia removal and total inorganic nitrogen removal stabilized at 99 % and 93 %, respectively, under a hydraulic retention time (HRT) of 6 h. High ammonia oxidation rate (~ 0.64 g N/g VSS/d) was achieved, with ~93 % ammonia conversing to nitrite before being reduced to nitrogen gas. Microbial analysis results revealed that Nitrosomonas (ammonia oxidizing bacteria) and Thauera (denitrifiers) were the dominant bacteria with key functional genes involved in the Nit/DNit. With an increase in the LLW loading, increased ammonia oxidation rates and biomass retention were also observed. This study demonstrated that granular sludge-based technology is feasible for mature LLW treatment.
Asunto(s)
Aguas del Alcantarillado , Contaminantes Químicos del Agua , Amoníaco , Análisis de la Demanda Biológica de Oxígeno , Reactores Biológicos/microbiología , Nitrógeno , Oxidación-Reducción , Aguas del Alcantarillado/microbiología , Aguas ResidualesRESUMEN
Objective: To explore the temperature distribution characteristics of the face, palms, feet and the trunk area of metabolic syndrome (MS) through infrared thermography (IRT) and provide evidence for the application of IRT in the assistant evaluation of MS population. Methods: We collected thermographs of 184 participants (91 males, 93 females) and further divided participants of each gender into 4 groups according to the number of abnormal metabolic indexes. Mean temperatures of 6 Region of Interests (ROIs) (face, anterior trunk, bilateral palms and dorsum of feet) were calculated. Comparisons of the mean temperatures between genders, among groups and ROIs were carried out. Results: Male participants had higher mean temperature in their face, palms (P<0.01) and dorsum of feet (P<0.05), and lower mean temperature in the anterior trunk (P<0.01). Female participants with MS had higher mean temperature in their palms and dorsum of feet (P<0.01) and lower mean temperature in the anterior trunk (P<0.01) than normal participants. Similar tendencies were shown in the mean temperature of the left palms and trunk of MS males. With the increase of the number of abnormal metabolic indexes, it seems that the mean temperature gradually increased in palms and dorsum of feet, and decreased in the anterior trunk. Conclusion: The thermograph of MS exhibits certain characteristics. This may help reveal the correlations between Infrared thermography and metabolic disorders.
Asunto(s)
Síndrome Metabólico , Cara , Femenino , Pie , Cabeza , Humanos , Masculino , Síndrome Metabólico/diagnóstico , TemperaturaRESUMEN
Jujube witches' broom disease (JWB), one of the most serious phytoplasma diseases, usually results in the destruction of Chinese jujube (Ziziphus jujuba Mill.). Although most jujube cultivars are sensitive to JWB, we found a few genotypes that are highly resistant to JWB. However, the molecular mechanism of phytoplasma resistance has seldom been studied. Here, we used Chinese jujube "T13," which has strong resistance to JWB, and a typical susceptible cultivar, "Pozao" ("PZ"), as materials to perform comparative transcriptome, hormone, and regulation analyses. After phytoplasma infection, the differential expression genes (DEGs) were detected at all three growth phases (S1, S2, and S3) in "PZ," but DEGs were detected only at the first growth phase in "T13." Meanwhile, no phytoplasma was detected, and the symptoms especially witches' broom caused by JWB were not observed at the last two growth phases (S2 and S3) in "T13." Protein-protein interaction analysis also showed that the key genes were mainly involved in hormone and reactive oxygen species (ROS) signaling. In addition, during the recovered growth phase in "T13" from S1 to S2, the level of hydrogen peroxide (H2O2) was significantly increased and then decreased from S2 to S3. Moreover, jasmonic acid (JA) was significantly accumulated in "PZ" diseased plants, especially at the S2 phase and at the S2 phase in "T13," while the content of salicylic acid (SA) decreased significantly at the S2 phase of "T13" compared to that in "PZ." The changes in H2O2 and JA or SA were consistent with the changes in their key synthesis genes in the transcriptome data. Finally, exogenous application of an SA inhibitor [1-aminobenzotriazole (ABT)] rescued witches' broom symptoms, while the contents of both JA and MeJA increased after ABT treatment compared to the control, demonstrating that exogenous application of an SA inhibitor rescued the symptoms of jujube after phytoplasma infection by decreasing the contents of SA and increasing the contents of JA and MeJA. Collectively, our study provides a new perspective on the transcriptional changes of Chinese jujube in response to JWB and novel insights that the crosstalk of JA and SA signaling communicated together to contribute to "T13" JWB resistance.
RESUMEN
Operational factors and microbial interactions affect the ecology in anaerobic digestion systems. From 12 lab-scale reactors operated under distinct engineering conditions, bacterial communities were found driven by temperature, while archaeal communities by both temperature and substrate properties. Combining the bacterial and archaeal community clustering patterns led to five sample groups (ambient, mesophilic low-solid-substrate, mesophilic, mesophilic co-digestion and thermophilic) for co-occurrence network analysis. Network topological properties were associated with substrate characteristics and hydrolysis-methanogenesis balance. The hydrolysis efficiency correlated (p < 0.05) with clustering coefficient positively and with normalized betweenness negatively. The influent particulate COD ratio and the relative differential hydrolysis-methanogenesis efficiency (Defficiency) correlated negatively with the average path length (p < 0.05). Individual genera's topological properties showed more connector genera in thermophilic network, representing stronger inter-module communication. Individual genera's normalized degree and betweenness revealed that lower-abundance genera (as low as 0.1%) could perform central hub roles and communication roles, maintaining the stability and functionality of the microbial community.
Asunto(s)
Reactores Biológicos , Consorcios Microbianos , Anaerobiosis , Archaea , Bacterias/genéticaRESUMEN
Hydrogen peroxide (H2O2) plays pivotal roles in various biological functions and pharmacological activities. High selectivity and sensitivity remain challenges for fluorescent probes to detection of H2O2 with a large stokes shift. Herein, a new "turn-on" fluorescent probe (DCM-C) was designed based on the mechanism of intramolecular charge transfer (ICT). In PBS buffer (10â¯mM, pH 7.4, with 20% DMSO, v/v), DCM-C exhibited high selectivity and sensitivity for H2O2 over other interfering analytes with a large stokes shift (187â¯nm), and the detection limit was as low as 35.5â¯nM. In addition, the probe was effective for detecting exogenous and endogenous H2O2 in living cells, and identifying stained in cytoplasm. Moreover, the probe has been used successfully for determining H2O2 in zebrafish by fluorescence imaging.