Fabrication and characterization of polymer optical waveguide Bragg grating for pulse signal sensing.

Polymer materials have the advantages of a low Young's modulus and low-cost preparation process. In this paper, a polymer-based optical waveguide pressure sensor based on a Bragg structure is proposed. The change in the Bragg wavelength in the output spectrum of the waveguide Bragg grating (WBG) is used to linearly characterize the change in pressure acting on the device. The polymer-based WBG was developed through a polymer film preparation process, and the experimental results show that the output signal of the device has a sensitivity of 1.275 nm/kPa with a measurement range of 0-12 kPa and an accuracy of 1 kPa. The experimental results indicate that the device already perfectly responds to a pulse signal. It has significant potential application value in medical diagnostics and health testing, such as blood pressure monitoring, sleep quality monitoring, and tactile sensing.

Salinity stress induces epigenetic alterations to the promoter of MsMYB4 encoding a salt-induced MYB transcription factor.

The transcriptomic response of plants to salinity stress is regulated in part by epigenetic alterations to gene promoter sequences. The transcription factor MsMYB4 is an important component of the response of alfalfa to salinity stress, but the involvement of epialleles of its encoding gene has not as yet been explored. Here, the MsMYB4 promoter was isolated using a genome walking approach in order to perform a deletion analysis to identify the region harboring the elements required for its stress inducibility. The analysis showed that these reside in the sequence lying between 739 and 336 nt up stream of the MsMYB4 translation start codon. The methylation status of the sequence around the MsMYB4 translation start site was altered by the imposition of salinity stress. The activation of MsMYB4 was associated with an increased level of histone H3K4 trimethylation and H3K9 acetylation in specific regions of the promoter sequence. Our results suggest a critical role for MsMYB4's activation by DNA methylation and/or histone modifications in response to salinity stress in alfalfa.

The expression of alfalfa MsPP2CA1 gene confers ABA sensitivity and abiotic stress tolerance on Arabidopsis thaliana.

Although clade A phosphatase 2Cs (PP2CAs) are well known to regulate abscisic acid (ABA) signaling, few members of this family have been identified in alfalfa so far. Here, the isolation and characterization of the gene MsPP2CA1 from alfalfa is described. Its transcription was found to be highly inducible by treatment with abscisic acid, salt, hydrogen peroxide and polyethylene glycol. The constitutive expression of MsPP2CA1 in Arabidopsis thaliana seedlings mitigates root growth imposed by either salinity or oxidative stress, while also raising the level of sensitivity to ABA during germination and early seedling development, and promoting stomatal closure. In transgenic plants, many ABA-dependent stress-responsive genes were activated, and the expressions of catalase and peroxidase which involved in reactive oxygen scavenging were promoted. MsPP2CA1 is suggested as a candidate for the genetic manipulation of salinity tolerance in legume species.

A wheat GTP-binding protein like gene reduces tolerance to low temperature in Arabidopsis.

Low temperature adversely affects plant growth and crop yield. The studies largely focus on cold stress (<4 °C), while the response upon low temperature higher than 4 °C is rarely documented so far. Here, we isolate a GTP-binding protein ß subunit like gene TaGPBL. TaGPBL is responsive to low temperature of 16 °C, and its ectopic overexpression in Arabidopsis results in more remarkable growth restriction under 16 °C, but has no effect under 22 °C. TaGBPL overexpression reduces the induction of cold-inducible genes and the activities of ROS scavengers and producers in lower temperature dependent manner. The data indicate that TaGBPL participates in the response to low temperature, which provides evidence for deepening our insight into the role of G-protein in temperature perception and signaling transduction.

[Analysis of histone modification of MtSERK1 during in vitro regeneration in Medicago truncatula].

Epigenetic modification, especially histone modification, plays an important role in maintaining plant genome stability, regulating gene expression and promoting regeneration in vitro. MtSERK1 is an important marker gene involved in establishing of embryogenic callus during in vitro regeneration of Medicago truncatula. In order to understand the regulation Epigenetic modification, especially histone modification, plays an important role in maintaining plant genome stability, regulating gene expression and promoting regeneration in vitro. MtSERK1 is an important marker gene involved in establishing of embryogenic callus during in vitro regeneration of Medicago truncatula. In order to understand the regulation relationship between dynamic histone modification and MtSERK1s expression during the processes of in vitro organogenesis, the expression of MtSERK1 was analyzed by qRT-PCR, and the modification status of H3K9me2, H3K4me3 and H3K9ac in the promoter region and different regions included in the gene body was analyzed by chromatin immunoprecipitation (ChIP). We found expression activation of MtSERK1 was related to the dynamic changes of histone H3K4me3 and H3K9ac in the 5' and 3' regions. This study will provide important theoretical guidance for understanding of the regulatory mechanism of MtSERK1 and also for establishing efficient genetic transformation system of Medicago truncatula.

Transcriptional profiling reveals that a MYB transcription factor MsMYB4 contributes to the salinity stress response of alfalfa.

MYB transcription factors are important regulators of the plant response to abiotic stress. Their participation in the salinity stress of the key forage legume species alfalfa (Medicago sativa) was investigated here by comparing the transcriptomes of the two cultivars Dryland (DL) and Sundory (SD), which differed with respect to their ability to tolerate salinity stress. When challenged by the stress, DL plants were better able than SD ones to scavenge reactive oxygen species. A large number of genes encoding transcription regulators, signal transducers and proteins involved in both primary and secondary metabolism were differentially transcribed in the two cultivars, especially when plants were subjected to salinity stress. The set of induced genes included 17 MYB family of transcription factors, all of which were subsequently isolated. The effect of constitutively expressing these genes on the salinity tolerance expressed by Arabidopsis thaliana was investigated. The introduction of MsMYB4 significantly increased the plants' salinity tolerance in an abscisic acid-dependent manner. A sub-cellular localization experiment and a transactivation assay indicated that MsMYB4 was deposited in the nucleus and was able to activate transcription in yeast. Based on this information, we propose that the MsMYB4 products is likely directly involved in alfalfa's response to salinity stress.