Breaking down barriers: live or dehydrated dietary whole black soldier fly larvae supplementation in slow growing chickens preserve meat quality and sensory traits.

This study investigated the effects of supplementing the diet of a slow-growing autochthonous chicken breed with dehydrated or live Black Soldier Fly Larvae (BSFL) on meat quality and sensory attributes. The research, conducted at the University of Turin, Italy, involved 144 male birds distributed in three experimental groups. The control group (C) was fed a basal diet in which soybean meal was completely substituted with alternative ingredients. The 2 experimental groups were administered a diet identical to the control group but supplemented with either whole dehydrated black soldier fly larvae (DL) or whole live black soldier fly larvae (LL) at a level equal to 5% expected daily feed intake of dry matter. We evaluated the following parameters: nutrient intake, slaughtering performance, physical and nutritional meat quality, fatty acid composition, proteomics, and sensory characteristics. The results demonstrated BSFL supplementation to have no detrimental effects on overall meat quality or sensory attributes. Specifically, there were no significant differences in physical meat quality parameters, nutritional composition, lipid oxidation, or protein digestibility between control and BSFL-fed groups. Fatty acid analysis revealed higher concentrations of lauric and myristic acids in BSFL-fed chicken breast (p < 0.005), suggesting potential nutritional benefits from the supplement. The proteomic analysis also showed no significant differences in the expression of abundant proteins in the breast meat between groups, indicating minimal physiological impact of BSFL supplementation. Overall, this study provides reassurance to consumers and industries about the suitability of BSFL as a sustainable feed supplement for poultry that also offers potential benefits in terms of optimizing the fatty acid profile of chicken meat.

Dietary processed former foodstuffs for broilers: impacts on growth performance, digestibility, hematobiochemical profiles and liver gene abundance.

BACKGROUND: The present experiment aimed to evaluate the effects of commercially processed former foodstuffs (cFF) as dietary substitutes of corn, soybean meal and soybean oil on the growth performance, apparent total tract digestibility (ATTD), hematobiochemical profiles, and liver gene abundance in broiler chickens. Two hundred one-day-old male ROSS-308 chicks were assigned to 4 dietary groups (5 replicates of ten birds per replicate) according to their average body weight (BW, 38.0 ± 0.11 g). All groups received a two-phase feeding program: starter, d 1-12 and grower, d 12-33. The control group (cFF0) was fed a standard commercial feed based on corn, soybean meal and soybean oil. The other three groups received diets in which the feed based on corn, soybean meal, and soybean oil was partially replaced with cFF at a substitution level of 6.25% (cFF6.25), 12.5% (cFF12.5) or 25% (cFF25) for the following 33 d. RESULTS: The growth performance data showed no differences in BW or average daily gain among groups, although the average daily feed intake decreased during the grower period (12-33 d) and over entire experimental period (1-33 d) in a linear manner as the cFF inclusion level rose (P = 0.026), positively affecting the gain to feed ratio (P = 0.001). The ATTD of dry matter of the cFF-fed groups were greater with respect to control group and increased throughout the experimental period, whereas the ATTD of ether extract linearly decreased with increasing levels of cFF-fed groups compared with control group and throughout the experimental period (P < 0.05). Additionally, a linear increase in the heterophil to lymphocyte ratio, serum cholesterol, triglycerides and alanine-aminotransferase were observed with increasing dietary levels of cFF (P < 0.05); however, no differences were observed in lipoprotein lipase or sterol regulatory element binding transcription factor gene abundance. CONCLUSIONS: The results of this experiment demonstrate that it is possible to incorporate cFF into nutritionally balanced diets for broiler chickens, even up to 25% substitution levels, for up to 33 d without adversely impacting the overall growth performance of male broiler chickens raised under commercial conditions. Further studies are essential to validate the hematological trait findings.

Organic medium-growing chickens fed live black soldier fly larvae: A welfare improvement study.

The overall beneficial effect of live black soldier fly larvae (BSFL) on the welfare of broiler chickens, turkeys, and laying hens has already been discussed in the literature. However, scant information is available regarding the benefits of feeding live BSFL to medium-growing chicken hybrids reared under organic/free-range conditions, and whose welfare is frequently cited as being inadequate. The aim of this research was to advance our knowledge of this topic. To this end, 240 label naked neck birds (Hubbard JA57 hybrid) were assigned, at 21 days of age, to four experimental groups (6 replicates/treatment, 10 chickens/replicate), created according to sex (M/F) and the provision of a 10% live BSFL dietary supplementation (control males, control females, larvae males, and larvae females), and raised until 82 days of age. We performed behavioural observations, a tonic immobility test, and an avoidance distance (AD) test. We assessed feather damage and cleanliness, hock burn, footpad dermatitis, and skin lesion scores, and determined the concentration of excreta corticosterone metabolites (ECM) and the heterophile to lymphocyte heterophile/lymphocyte (H/L) ratio. The behavioural observations demonstrated increased physical and foraging activity (p < 0.05) in the live BSFL administered groups compared with C ones, providing valuable data on the explorative and recreational behaviour of this chicken genotype. The results also evidenced the usefulness of live BSFL as a fear reducer in females, as those receiving the BSFL supplement moved closer to the operator during the AD test (p < 0.01). No physical injuries or damage were observed on the birds, regardless of whether they received the BSFL supplementation or not. The ECM were unaffected by BSFL supplementation, while the H/L ratio was higher in the larvae groups than in the control ones (p = 0.050). In conclusion, live BSFL provision could constitute a powerful tool for improving life quality in medium-growing chickens. Further research is required to clarify the stress modulation role of live BSFL on poultry production.

Insect live larvae as a new nutritional model in duck: effects on gut health.

BACKGROUND: This study aimed to evaluate the effects of Hermetia illucens (Black soldier fly-BSF) and Tenebrio molitor (Yellow mealworm-YMW) live larvae as a new nutritional model on duck's gut health, considering gut histomorphometry, mucin composition, cytokines transcription levels, and microbiota. A total of 126, 3-days-old, females Muscovy ducks were randomly allotted to three dietary treatments (6 replicates/treatment, 7 birds/pen): (i) C: basal diet; (ii) BSF: C + BSF live larvae; (iii) YMW: C + YMW live larvae. BSF and YMW live larvae were administered on top of the basal diet, based on the 5% of the expected daily feed intake. The live weight, average daily gain, average daily feed intake and feed conversion ratio were evaluated for the whole experimental period. On day 52, 12 ducks/treatment (2 birds/replicate) were slaughtered and samples of duodenum, jejunum, ileum, spleen, liver, thymus and bursa of Fabricius were collected for histomorphometry. Mucin composition was evaluated in the small intestine through histochemical staining while jejunal MUC-2 and cytokines transcription levels were evaluated by rt-qPCR. Cecal microbiota was also analyzed by means of 16 S rRNA gene sequencing. RESULTS: Birds' growth performance and histomorphometry were not influenced by diet, with a proximo-distal decreasing gradient from duodenum to ileum (p < 0.001), respecting the physiological gut development. Mucin staining intensity and MUC-2 gene expression did not vary among dietary treatments, even though mucin intensity increased from duodenum to ileum, according to normal gut mucus physiology (p < 0.001). Regarding local immune response, IL-6 was higher in YMW group when compared to the other groups (p = 0.009). Insect live larvae did not affect cecal microbiota diversity, but BSF and YMW groups showed a higher presence of Helicobacter, Elusimicrobium, and Succinatimonas and a lower abundance of Coriobacteriaceae and Phascolarctobacterium compared to C birds (p < 0.05). CONCLUSIONS: The use of BSF and YMW live larvae as new nutritional model did not impair gut development and mucin composition of Muscovy ducks, but slightly improved the intestinal immune status and the microbiota composition by enhancing regulatory cytokine IL-6 and by increasing minor Operational Taxonomic Units (OTUs) involved in short-chain fatty acids production.

Lauric acid content in intramuscular fat is a reliable indicator of black soldier fly larvae meal consumption in Muscovy ducks.

The present research investigated if intramuscular fatty acid (FA) profile could distinguish meat from ducks fed with black soldier fly larvae meal (BSFLM) during fattening. By using stepwise linear discriminant analysis on FA profiles of 96 meat samples, lauric acid (C12:0) was found to be the best predictor, accurately differentiating samples with only two misclassifications. The Fisher classification functions indicated that breast samples with lauric acid content above 0.222 % in intramuscular fat would be classified as BSFLM-fed. The Fisher classification functions were also effective in other poultry species fed BSFLM, with only two misclassified samples out of 42 samples from a dataset compiled from published papers. Misclassifications were linked to an unexpected lauric acid content in the intramuscular fat. In conclusion, given that BSFLM is the only relevant source of lauric acid in the diet, this fatty acid could identify meat from BSFLM-fed ducks and possibly other poultry species, warranting further exploration of other FA as potential identifiers of BSFLM-fed poultry.

Former foodstuffs in feed: a minireview of recent findings.

The sustainability of all productive activities, including livestock farming, becomes a fundamental challenge in the current scenario. Livestock production faces both old and new challenges related to climate change, food safety, and feed-food competition. The latter aspect has recently become a hot topic, and many researchers are turning their attention to this issue. According to circular economy principles, former foodstuffs have characteristics that make them a promising source of raw material for animal feed. The main objective of the present review is to provide a brief overview of the most recent studies (published between 2016 and 2022) addressing the dietary inclusion of former foodstuffs for livestock. The articles analyzed cover key findings from both in vitro and in vivo studies of former foodstuffs included in the diets for pigs, cows, and broilers, and assess the associated safety aspects. The articles provide information on livestock performances and product quality, as well as feed digestibility, fecal microbiota, and blood analysis. Although the evidence supports the inclusion of former foodstuffs in livestock diets as a safe, effective, and sustainable ingredient, this analysis of the most recent literature also highlights gaps in our knowledge that need to be filled. The present overview will help researchers plan future research and standardize and promote the inclusion of former food products in livestock diets.

Does the provision of live black soldier fly and yellow mealworm larvae improve Muscovy duck welfare?

BACKGROUND: The provision of environmental enrichments to Muscovy ducks could reduce the expression of the aggressive behaviors. The aim of the present study was to evaluate the effects of black soldier fly (BSF) and yellow mealworm (YM) live larva provision on Muscovy duck performance, excreta corticosterone metabolites (ECM), behavior, and blood parameters. METHODS: A total of 126 3-day-old female Muscovy ducklings were allotted to 18 pens (6 replicates/treatment, 7 birds/pen) and assigned to 3 experimental treatments: a control group fed commercial feed, and two experimental treatments fed commercial feed plus the 5% (based on the expected daily feed intake, as fed basis) of BSF and YM live larvae (BSF and YM groups, respectively). A two-phase feeding program was applied: starter (from 3 to 31 days of age) and grower-finisher (from 32 to 55 days of age). The live weight, average daily gain, average daily feed intake, and feed conversion ratio were calculated. Larva consumption times were collected, and video recordings were performed during 3 periods (P) each day: the hour before (P1), during (P2), and after (P3) the larva administration. ECM were evaluated at 3, 31, and 55-day-old. Finally, the total red and white blood cell counts, serum proteins, lipids, and liver and renal function serum enzymes were evaluated on 12 birds/treatment. RESULTS: The experimental treatment did not affect the growth performance of the birds (P > 0.05). Larva consumption times were always similar between the two insect species, except at 14-18 days of age, were BSF larvae were consumed faster than YM larvae (P < 0.001). The birds showed less walking activity during P2, and preening behavior increased in YM birds during P3. The C birds increased the attack behavior over the weeks (P < 0.05). During weeks 1-3 the YM group reduced the attack frequency (P1 > P3; P < 0.05). Finally, the provision of live BSF and YM larvae significantly reduced the ECM at 55 days of age and the heterophil to lymphocyte ratio (P < 0.05). CONCLUSIONS: Live BSF and YM larva supplementation in Muscovy duck improves duck welfare, without impairing birds' growth performance.

Lactiplantibacillus plantarum, lactiplantibacillus pentosus and inulin meal inclusion boost the metagenomic function of broiler chickens.

BACKGROUND: The inclusion of alternative ingredients in poultry feed is foreseen to impact poultry gut microbiota. New feeding strategies (probiotics/prebiotics) must be adopted to allow sustainable productions. Therefore, the current study aimed to use metagenomics approaches to determine how dietary inclusion of prebiotic (inulin) plus a multi-strain probiotic mixture of Lactiplantibacillus plantarum and Lactiplantibacillus pentosus affected microbiota composition and functions of the gastro-intestinal tract of the broilers during production. Fecal samples were collected at the beginning of the trial and after 5, 11 and 32 days for metataxonomic analysis. At the end of the trial, broilers were submitted to anatomo-pathological investigations and caecal content was subjected to volatilome analysis and DNAseq. RESULTS: Probiotic plus prebiotic inclusion did not significantly influence bird performance and did not produce histopathological alterations or changes in blood measurements, which indicates that the probiotic did not impair the overall health status of the birds. The multi-strain probiotic plus inulin inclusion in broilers increased the abundance of Blautia, Faecalibacterium and Lachnospiraceae and as a consequence an increased level of butyric acid was observed. In addition, the administration of probiotics plus inulin modified the gut microbiota composition also at strain level since probiotics alone or in combination with inulin select specific Faecalibacterium prausnitzi strain populations. The metagenomic analysis showed in probiotic plus prebiotic fed broilers a higher number of genes required for branched-chain amino acid biosynthesis belonging to selected F. prausnitzi strains, which are crucial in increasing immune function resistance to pathogens. In the presence of the probiotic/prebiotic a reduction in the occurrence of antibiotic resistance genes belonging to aminoglycoside, beta-lactamase and lincosamide family was observed. CONCLUSIONS: The positive microbiome modulation observed is particularly relevant, since the use of these alternative ingredients could promote a healthier status of the broiler's gut.

Effect of Dietary Organic Acids and Botanicals on Metabolic Status and Milk Parameters in Mid-Late Lactating Goats.

The microencapsulated mixture of organic acids and pure botanicals (OA/PB) has never been evaluated in goats. The aim of this study was to extend the analysis to mid-late lactating dairy goats, evaluating the effects of OA/PB supplementation on the metabolic status, milk bacteriological and composition characteristics, and milk yield. Eighty mid-late lactating Saanen goats were randomly assigned to two groups: one group was fed the basal total balanced ration (TMR) (CRT; n = 40) and the other was fed a diet that was TMR supplemented with 10 g/head of OA/PB (TRT; n = 40) for 54 days during the summer period. The temperature-humidity index (THI) was recorded hourly. On days T0, T27, and T54, the milk yield was recorded, and blood and milk samples were collected during the morning milking. A linear mixed model was used, considering the fixed effects: diet, time, and their interaction. The THI data (mean ± SD: 73.5 ± 3.83) show that the goats did not endure heat stress. The blood parameters fell within the normal range, confirming that their metabolic status was not negatively influenced by OA/PB supplementation. OA/PB increased the milk fat content (p = 0.04) and milk coagulation index (p = 0.03), which are effects that are looked on as favorable by the dairy industry in relation to cheese production.

Effects of feeding a thermomechanical, enzyme-facilitated, coprocessed yeast and soybean meal on growth performance, organ weights, leg health, and gut development of broiler chickens.

The development of a healthy gut during prestarter and starter phases is crucial to drive chicken's productivity. This study aimed to evaluate the effects of a thermomechanical, enzyme-facilitated, coprocessed yeast and soybean meal (pYSM) on growth performance, organ weights, leg health, and gut development in broiler chickens. A total of 576 as-hatched broiler chicks were randomly allotted to 3 dietary treatments (8 replicates/treatment, 24 chickens/replicate): a control group (C) without the pYSM, a treatment group 1 (T1), in which the pSYM was included at 20, 10, 5, 0, and 0% levels in the prestarter, starter, grower, finisher I, and finisher II feeding phases, respectively, and a treatment group 2 (T2), in which the pSYM was included at 5, 5, 5, 0, and 0% levels in each feeding phase. On d 3 and 10, 16 broilers/treatment were euthanized. The T1 broilers tended to show higher live weight (d 3 and 7) and average daily gain (prestarter and starter phases) than the other groups (P ≤ 0.10). Differently, pYSM-based diets did not influence the growth performance of the other feeding phases and the whole experimental period (P > 0.05). Relative weights of pancreas and liver were also unaffected by pYSM utilization (P > 0.05). Litter quality tended to have higher average scores in C group (P = 0.079), but no differences were observed for leg health (P > 0.05). Histomorphometry of gut, liver, and bursa of Fabricius was not affected by diet (P > 0.05). Gut immunity was driven to an anti-inflammatory pattern, with the reduction of IL-2, INF-γ, and TNF-α in the duodenum of treated birds (d 3, P < 0.05). Also, MUC-2 was greater in the duodenum of C and T2 group when compared to T1 (d 3, P = 0.016). Finally, T1-fed chickens displayed greater aminopeptidase activity in the duodenum (d 3 and 10, P < 0.05) and jejunum (d 3, P < 0.05). Feeding high levels of pYSM (10-20%) to broilers in the first 10 d tended to improve growth performance in the prestarter and starter phases. It also positively downregulated proinflammatory cytokines during the first 3 d, as well as stimulated the aminopeptidase activity in the prestarter and starter periods.

Welfare implications for broiler chickens reared in an insect larvae-enriched environment: Focus on bird behaviour, plumage status, leg health, and excreta corticosterone.

The use of insect live larvae as environmental enrichment has recently been proposed in broiler chickens, but the concomitant administration of black soldier fly (BSF) and yellow mealworm (YM) has never been tested yet. Therefore, the present study aims to evaluate the effects of live BSF and YM larvae as environmental enrichments for broiler chickens by means of plumage status, behaviour, leg health, and excreta corticosterone metabolites (CM). A total of 180 4-day old male Ross 308 broiler chickens were randomly distributed in 3 experimental treatments (6 replicates/treatment, 10 birds/replicate) and fed for 35 days as follows: 1) control (C, commercial feed), 2) BSF: C + 5% of the expected daily feed intake [DFI] live BSF larvae and 3) YM: C + 5% of the expected DFI live YM larvae. Feathering, hock burn (HB) and footpad dermatitis (FPD) scores (end of the trial), as well as behavioural observations (beginning of the trial [T0] and every 11 days [T1, T2 and T3] during morning, larvae intake and afternoon) through video recordings, were assessed, and excreta samples collected to evaluate the CM. Feathering, HB and FPD scores, and excreta CM were unaffected by insect live larvae administration (p > 0.05). In the morning, the insect-fed birds displayed higher stretching, wing flapping, ground pecking (at T1 and T3), as well as lower preening (at T1 and T2), than the C group (p < 0.05). During the larvae intake, higher scratching, wing flapping and ground pecking, as well as lower stretching, preening and laying down, were observed in the insect-fed (scratching, stretching and laying down) or YM-fed (wing flapping, ground pecking and preening) groups than the C birds (p < 0.05). In the afternoon, insect live larvae administration increased wing flapping (YM) and laying down (BSF and YM), as well as decreased ground pecking (YM, p < 0.05). In conclusion, the administration of insect live larvae as environmental enrichment (especially YM) was capable of positively influencing the bird welfare through the stimulation of foraging behaviour, increase in activity levels, and reduction in bird frustration, without affecting the plumage status, leg health, and excreta CM.

Carcass Yields and Meat Composition of Male and Female Italian Slow-Growing Chicken Breeds: Bianca di Saluzzo and Bionda Piemontese.

The slaughter performance and meat quality of two native Italian chicken breeds, Bionda Piemontese (BP, n = 64) and Bianca di Saluzzo (BS, n = 64), were investigated. Two-way ANOVA, considering breed, sex, and their interaction, was used to compare the properties of birds slaughtered at 5, 6, 7, and 8 months of age. Subsequently, data were analyzed using one-way ANOVA and the Duncan test to evaluate the differences between slaughter ages. The BP breed produced a better carcass yield than BS at 5, 7, and 8 months of age (p < 0.05). Breast moisture and crude protein contents were influenced by gender, and were higher in males than in females (p < 0.05). By contrast, the crude fat content was higher in females than in males (p < 0.05). The saturated fatty acid content of breast meat increased as the birds aged in both breeds (p < 0.05). The polyunsaturated fatty acid content of both breast and thigh meat was higher in males than in females (p < 0.001 and p < 0.05, respectively). In general, slaughtering at 7 months was associated with the best slaughter and meat quality characteristics in both breeds. Moreover, from a nutritional point of view, the characteristics of the meat from male birds were preferable to those of meat from females.

Black soldier fly larvae used for environmental enrichment purposes: Can they affect the growth, slaughter performance, and blood chemistry of medium-growing chickens?

Introduction: This research has been aimed at evaluating the effects of live black soldier fly larvae (BSFL) (Hermetia illucens) on the growth, slaughtering performance, and blood parameters of medium-growing chickens. Methods: A total of 240, 28-day-old, Label Rouge Naked Neck chickens were allotted to four experimental groups, according to the gender (males-females) and to the absence (control group, C) or presence (larvae group, L) of a dietary supplementation with 10% live BSFL, on the basis of the expected average daily feed intake (ADFI) (6 replicates/diet, 10 chickens/replicate). The birds were weighed weekly, and the feed consumption was recorded to calculate the average live weight, feed conversion ratio (FCR), average daily gain (ADG), and the ADFI. At 82 days of age, 2 birds/replicate (12 birds/diet) were selected and slaughtered. The blood samples were collected, and the carcass traits (carcass, breast, thigh, and organ weights and yields) were assessed. Results and discussions: Overall, the administered live BSFL did not impair the growth and slaughtering performance, or the blood traits, while the C females showed a better FCR than the treated ones (P < 0.05). The live BSFL consumption time was longer for the females than for the males (P < 0.001). The weight of the immune organs (spleen and bursa of Fabricius) increased as the live BSFL supplementation increased (P < 0.05). Furthermore, the provision of live BSFL reduced the gamma glutamyl transferase (GGT, U/l) activity content in the blood (P < 0.05). Finally, both the leukocytes (%) and the monocytes (%) were more abundant in the C groups than in the larvae ones (P < 0.05 and P < 0.01, respectively). In short, the supplementation of live BSFL can be used successfully as an environmental enrichment, without affecting the growth performance of male birds. Furthermore, the immune organ activity could be enhanced by the provision of live BSFL.

Effect of Insect Live Larvae as Environmental Enrichment on Poultry Gut Health: Gut Mucin Composition, Microbiota and Local Immune Response Evaluation.

The aim of this study was to evaluate the effect of Hermetia illucens (HI) and Tenebrio molitor (TM) live larvae as environmental enrichment on the mucin composition, local immune response and microbiota of broilers. A total of 180 four-day-old male broiler chickens (Ross 308) were randomly allotted to three dietary treatments (six replicates/treatment; ten animals/replicate): (i) control (C); (ii) C+HI; (iii) C+TM. Live larvae were distributed based on 5% of the expected daily feed intake. At slaughter (39 days of age), samples of duodenum, jejunum and ileum (twelve animals/diet) were submitted to mucin histochemical evaluation. Expression of MUC-2 and cytokines was evaluated by rt-qPCR in jejunum. Mucin staining intensity was not influenced by diet (p > 0.05); however, this varied depending on the intestinal segment (p < 0.001). No significant differences were recorded for IL-4, IL-6 TNF-α, MUC-2 and INF-γ gene expression in jejunum, while IL-2 was lower in the TM group compared to HI and C (p = 0.044). Caecal microbiota showed higher abundance of Clostridium, Saccharibacteria and Victivallaceae in the HI group, while Collinsella was higher in the TM group. The results suggested that live insect larvae did not impair mucin composition or local immune response, and can slightly improve caecal microbiota by enhancing a minor fraction of short chain fatty acid-producing taxa.

Black soldier fly and yellow mealworm live larvae for broiler chickens: Effects on bird performance and health status.

The commercial broiler chicken strains are the result of successful selection programmes. Most of the problems related to welfare arise from the high growth rate and body weight. The use of environmental enrichments in intensive farming could have a positive effect on birds by increasing animal welfare. The aim of the study was to evaluate the effects of Hermetia illucens (HI) and Tenebrio molitor (TM) live larvae in the diets of broiler chickens on growth performance, carcass yield and health status. A total of 180 four-day-old male broiler chickens (Ross 308) were randomly allotted to 18 pens. Each pen was assigned to one of the three dietary treatments (6 replicates/treatment, 10 birds/replicate) as follows: (i) control diet (C): commercial feed (two feeding phases: starter [4-11 days] and grower [12-38 days]), (ii) HI: C + 5% of the expected daily feed intake (DFI) HI live larvae (calculated on dry matter [DM]) and (iii) TM: C + 5% of DFI TM live larvae (DM). At 39 days of age, birds were slaughtered. Growth performance parameters were overall not affected by dietary treatments, except for the grower phase feed conversion ratio (FCR) and the overall FCR being better in the TM broilers than the others (p < 0.01). No differences were observed for slaughtering performance and haematological and serum parameters, except for the spleen relative weight being higher (p < 0.01) in the birds administered with larvae when compared to the C group. Gut morphometric indexes and histopathological alterations were not influenced by insect larvae administration. In conclusion, the administration in limited quantities of HI and TM live larvae as environmental enrichment has no negative effects on broiler chicken growth performance and health status. A behavioural study could confirm that live insect larvae represent a novel natural environmental enrichment in broiler farming.

Modified Black Soldier Fly Larva Fat in Broiler Diet: Effects on Performance, Carcass Traits, Blood Parameters, Histomorphological Features and Gut Microbiota.

In this study, a total of 200 male broiler chickens (Ross 308) were assigned to four dietary treatments (5 pens/treatment and 10 birds/pen) for two feeding phases: starter (0-11 days of age) and grower-finisher (11-33 days of age). A basal diet containing soy oil (SO) as added fat was used as control group (C), tested against three experimental diets where the SO was partially substituted by BSF larvae fat (BSF) or one of two types of modified BSF larvae fat (MBSF1 and MBSF2, respectively). The two modified BSF larvae fats had a high and low ratio of monobutyrin to monoglycerides of medium chain fatty acid, respectively. Diet did not influence the growth or slaughter performance, pH, color, or the chemical composition of breast and thigh muscles, gut morphometric indices, or histopathological alterations in all the organs. As far as fecal microbiota are concerned, MBSF1 and MBSF2 diets reduced the presence of Clostridium and Corynebacterium, which can frequently cause infection in poultry. In conclusion, modified BSF larva fat may positively modulate the fecal microbiota of broiler chickens without influencing the growth performance and intestinal morphology or showing any adverse histopathological alternations.

Insects as Feed for Farmed Poultry: Are Italian Consumers Ready to Embrace This Innovation?

The inclusion of insects as a protein source in feed production is not only related to technical, economical, and normative restrictions but is also affected by consumer acceptance. In this study, we evaluated consumers' attitudes, intention to purchase and eat, and willingness to pay for meat obtained from a farmed duck fed with insect-based meal or a live insect diet. We conducted a survey among a sample of 565 consumers to test the effects of information about the benefits of using insects as feed on consumers' attitudes towards animal-based products fed with insects. Providing information on the sustainability and nutritional benefits of using insects as feed increased both attitude towards and intention to purchase and eat meat products made from animals fed with insects. In the treatment group, we found a significant reduction from 21.9 to 14.0% in those who wanted to be compensated for buying a duck fed with an insect-based meal and an increase in those willing to pay the same price-from 64.9 to 72.7%. The information treatment significantly increased the intention to eat such products, suggesting that increasing consumers' knowledge might help in reducing the fears and misconceptions around the topic of using insects as a feed source.

Overview of Native Chicken Breeds in Italy: Conservation Status and Rearing Systems in Use.

The most reared species of farm animal around the world is the chicken. However, the intensification of livestock systems has led to a gradual increase in the concentration of a limited number of breeds, resulting in substantial erosion to the genetic pool. The initial step of an 'animal conservation program' entails establishing the actual conservation statuses of the breeds concerned in a defined area; in this case, in Italy. To this end, a survey of breeds was performed by means of a census questionnaire divided into two parts. The first part collected information on breeds, breeders, housing facilities, and management aspects, the results of which are presented here. The second part of the questionnaire regarded chicken products and their markets, and these data will be reported in a second paper. The breed status of six chicken breeds was shown to be exceptionally worrying, with total numbers ranging from just 18 to 186 birds. Population sizes exceeding 1000 birds was identified for just four breeds, the maximum being 3400. Some improvements in status were noted in relation to breeds which had been the subject of conservation efforts in the past. The two most common breeds reported are the Bionda Piemontese, a double-purpose breed, and the Livorno egg-laying hen. Collo Nudo Italiano, Millefiori Piemontese, Pollo Trentino, and Tirolese chicken breeds and the Castano Precoce turkey breed were not listed by breeders at all. The most reported turkey breeds are the Bronzato Comune and the Ermellinato di Rovigo. The population sizes of native Italian poultry breeds were shown to be generally poor. Italian poultry farmers and the population at large are largely ignorant about indigenous poultry breeds. Thus, promoting the virtues of Italian breeds would help their conservation by encouraging breeders to rear these birds and consumers to buy their products. The identification of strategies to facilitate access to pure breed birds is essential, and will require the collaboration of university research centers, public entities, and breeders. The results presented in this paper constitute the initial part of a more complex conservation program.

Overview of Native Chicken Breeds in Italy: Small Scale Production and Marketing.

The intensive use of high-performing strains in poultry production has led to the extinction of several autochthonous chicken breeds and, consequently, loss of genetic variability. Interest in saving biodiversity is growing rapidly and has become a major objective worldwide. The aim of this study was to shed light on the production trends of native Italian poultry breeds and the related market. A questionnaire, which asked about the production cycles, the number of animals and table eggs produced per year and their retail prices was completed by 121 breeders across Italy. The surveyed breeders were divided into two categories: breeders conducting an agrozootechnical farm, referred to as 'farmers' (F); and breeders keeping chickens as backyard poultry, referred to as 'fancy breeders' (FB). Analysis of the data acquired indicated that animals were mainly slaughtered between 6 and 12 months of age, with F processing more animals per year. The same production trend was observed for table eggs. The recorded retail prices of native chicken products were higher than those for conventional products, but similar to those reported for valuable niche poultry products, such as the Poulet de Bresse in France and organic eggs. Knowledge about these highly valuable markets should be used to encourage the use of local breeds in alternative poultry farming and help protect biodiversity.

Performance of Slow-Growing Male Muscovy Ducks Exposed to Different Dietary Levels of Quebracho Tannin.

The study of the nutritional effects of tannins is complex due to the large chemical diversity; consequently, in poultry nutrition the biological responses may vary greatly. The aim of the present study was to evaluate the effect of different levels of dietary Quebracho tannins (QT) on growth and production performance in slow-growing type Muscovy ducks. For this purpose, a 42-d trial was carried out on 126 male ducks (42-d old at start), fed on three levels of dietary QT inclusion in the diet (0% as control diet, vs. 1.5% vs. 2.5% on an as fed basis). Birds were reared under free-range conditions. A linear increase in feed intake as a function of QT inclusion in the diet was observed (p < 0.05). No difference as to final body weight, overall average daily weight gain (ADG) and total feed conversion ratio (FCR) in relation to dietary treatments was observed. Carcass yields were positively improved in QT birds (p < 0.05). No adverse responses were recorded in total blood protein and liver weight. Dietary QT might be safely used up by to 2.5% in 42- to 84-d aged male Muscovy ducks.