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Bacillus spore enumeration using flow cytometry: A proof of concept for probiotic application.

Genovese, Maxime; Poulain, Edith; Doppler, Florie; Toussaint, Renaud; Boyer, Mickaël.

J Microbiol Methods ; 190: 106336, 2021 11.

Article En | MEDLINE | ID: mdl-34560161

Use of flow cytometry (FCM) for bacteria quantification is growing in the food industry. We report here a FCM method using a double-staining LDS751/SYTO24 for the quantification of probiotic Bacillus viable cells and its spores, with potential application for the control of commercial product specifications.

Bacillus/isolation & purification , Bacterial Load/methods , Flow Cytometry/methods , Spores, Bacterial/isolation & purification , Microbial Viability , Probiotics , Staining and Labeling