Ibuprofen degradation by mixed bacterial consortia: Metabolic pathway and microbial community analysis.

Degradation of ibuprofen, one of the most consumed drugs globally, by a mixed bacterial consortium was investigated. A contaminated hospital soil was used to enrich a bacterial consortium possessing the ability to degrade 4 mg/L ibuprofen in 6 days, fed on 6 mM acetate as a supplementary carbon source. Maximum ibuprofen degradation achieved was 99.51%, and for optimum ibuprofen degradation modelled statistically, the initial ibuprofen concentration, and temperature were determined to be 0.515 mg/L and 35 °C, respectively. The bacterial community analyses demonstrated an enrichment of Pseudomonas, Achromobacter, Bacillus, and Enterococcus in the presence of ibuprofen, suggesting their probable association with the biodegradation process. The biodegradation pathway developed using open-source metabolite predictors, GLORYx and BioTransformer suggested multiple degradation routes. Hydroxylation and oxidation were found to be the major mechanisms in ibuprofen degradation. Mono-hydroxylated metabolites were identified as well as predicted by the bioinformatics-based packages. Oxidation, dehydrogenation, super-hydroxylation, and hydrolysis were some other identified mechanisms.

Nickel adsorbed algae biochar based oxygen reduction reaction catalyst.

Lately, the bio electrochemical systems are emerging as an efficient wastewater treatment and energy conversion technology. However, their scaling-up is considerably restrained by slow-rate of cathodic oxygen reduction reaction (ORR) or otherwise by the high cost associated with the available efficient ORR catalysts. In this investigation, a cost-effective and eco-friendly approach for synthesizing Ni based ORR catalyst utilizing biosorption property of microalgae is accomplished. The synthesised Ni adsorbed algal biochar (NAB) served as an efficient cathode catalyst for enhancing ORR in a microbial carbon-capture cell (MCC). On increasing the initial concentration of Ni2+ in the aqueous medium from 100 mgL-1 to 500 mgL-1, the biosorption capacity was found to increase from 3 mgg-1 to 32 mgg-1 of algae cell. The MCC operated with NAB based cathode catalyst loading of 2 mgcm-2 exhibited 3.5 times higher power density (4.69 Wm-3) as compared to the one with commercial activated carbon. A significant organic matter removal (82 %) in the anodic chamber with simultaneous algal biomass productivity in the cathodic chamber was attained by MCC with cathode loaded with 2 mgcm-2 of NAB. Hence, this easily synthesised low-cost catalyst, out of waste stream, proved its ability to improve the performance of MCC.

Waste coconut shell-derived carbon monolith as an efficient binder-free cathode for electrochemical advanced oxidation treatment of endocrine-disrupting compounds.

Discharge of endocrine-disrupting compounds such as methylparaben (MePa) into natural water bodies deteriorates the aquatic ecosystem. In this regard, electrochemical oxidation (EO) and electro-Fenton (EF) processes are acknowledged as effective methods to eliminate biorecalcitrant compounds from different wastewater matrices. In these systems, the H2O2-producing ability of carbon-based cathodes is put to advantage for producing homogenous hydroxyl radicals by simulating Fenton's reaction, which dramatically augments the contaminant removal efficiency. However, commercial carbon based cathodes are not economically affordable, especially for voluminous treatment. Hence in the present work, waste-derived carbonised coconut shell (CCS) monolith was employed as a cathode in EO and EF treatment of MePa. Almost the entire MePa with initial concentration of 10 mg/L was removed in 60 min by EO and 45 min by EF process at neutral pH, applied current density of 7.5 mA/cm2, NaCl concentration of 1.0 g/L and 10 mg/L of Fe2O3 dosing. The MePa removal efficiency of the CCS cathode-fitted system after 60 min was better than the commercial graphite plate and Ti-based mixed metal oxide employing system due to higher H2O2 electrosynthesis (H2O2 = 9.0 ± 0.6 mg/L after 60 min). Moreover, the same setup was used for treating 10 mg/L of MePa-spiked real sewage and demonstrated MePa and total organic carbon removal efficiency of 80.16 ± 2.31% and 37.42 ± 3.50%, respectively, in 45 min. Further, the CCS-mediated EF treatment achieved >90% removal of MePa for eight continuous batch cycles and recorded a current density drop of just 0.23% per cycle. The degradation pathway and toxicity assessment of the intermediates using the Ecological Structure Activity Relationships (ECOSAR) tool supported the eco-friendliness of the current treatment scheme.

Nickel-iron-driven heterogenous bio-electro-fenton process for the degradation of methylparaben.

Discharge of emerging contaminants such as parabens in natural water bodies is a grievous concern. Among parabens, methylparaben (MP) is most prevalent due to its extensive usage in personal care and food products and has been purported to trigger hormonal-related diseases. In this regard, the bio-electro-Fenton (BEF) process garners attention for remediating refractory compounds because of its ability to generate in situ hydroxyl radicals (•OH) utilising the energy harvested from electroactive microorganisms. In the present investigation, a Ni-Fe-driven heterogenous BEF system (BEF-MFC) was used to degrade MP from different matrices. At neutral catholyte pH, 99.54 ± 0.22% of MP was removed from an initial concentration of 10 mg/L in 240 min of retention time with an estimated treatment cost of about 1.01 $/m3. The removal rate ameliorated when the catholyte pH was dropped to 3.0 and by imposing an external voltage of 0.5 V, requiring just 120 min to achieve comparable MP removal efficiencies. However, catalyst leaching was higher at acidic pH (leaching of Fe ions = 0.44 mg/L and Ni ions = 0.06 mg/L) and applying external voltage increased the treatment cost slightly to 1.08 $/m3. Further, treatment of 10 mg/L MP-spiked real wastewater at pH of 7.0 with the BEF-MFC attained 85.70 ± 3.30% and 56.50 ± 1.70% reduction in MP and total organic carbon, respectively, in 240 min. In addition, a maximum power density of 205.90 ± 2.27 mW/cm2 was harvested in the BEF-MFC; thus, portraying the dual benefit of Ni-Fe heterogeneous catalyst. Even though, Ni-Fe performed reasonably well as Fenton-cum-cathode catalyst, future endeavours should be poised to fine-tune catalysts to accelerate H2O2 and •OH generation, which will reinforce the scalability of this system.

Graphene and biochar-based cathode catalysts for microbial fuel cell: Performance evaluation, economic comparison, environmental and future perspectives.

Microbial fuel cells (MFCs) have been the prime focus of research in recent years because of their distinctive feature of concomitantly treating and producing electricity from wastewater. Nevertheless, the electrical performance of MFCs is hindered by a protracted oxygen reduction reaction (ORR), and often a catalyst is required to boost the cathodic reactions. Conventional transition metals-based catalysts are expensive and infeasible for field-scale usage. In this regard, carbon-based electrocatalysts like waste-derived biochar and graphene are used to enhance the commercialisation prospects of MFC technology. These carbon-catalysts possess unique properties like superior electrocatalytic activity, higher surface area, and high porosity conducive to ORR. Theoretically, graphene-based cathode catalysts yield superior results than a biochar-derived catalyst, though at a higher cost. In contrast, the synthesis of waste-extracted biochar is economical; however, its ability to catalyse ORR is debatable. Therefore, this review aims to make a side-by-side techno-economic assessment of biochar and graphene-based cathode catalyst used in MFC to predict the relative performance and typical cost of power recovery. Additionally, the life cycle analysis of the graphene and biochar-based materials has been briefly discussed to comprehend the associated environmental impacts and overall sustainability of these carbo-catalysts.

Enzymatic cell disruption followed by application of imposed potential for enhanced lipid extraction from wet algal biomass employing photosynthetic microbial fuel cell.

Solvent-free algal cell lysis using fungal enzyme for enhanced lipid recovery diminishes per unit production cost of algal biodiesel. In this investigation, a triple chamber photosynthetic microbial fuel cell (PMFC) was fabricated, where positive potential was imposed in the extraction chamber to draw the negatively charged lipid ions from the cathodic chamber. Under optimum imposed potential of + 3.0 V (vs standard hydrogen electrode) and with 3.5% (v/v) dosage of fungal enzyme in to the algal consortium of cathodic chamber, a maximum of 79.0% of lipid was recovered. Additionally, enzyme-assisted de-oiled algal biomass was applied in the anodic chamber to function as substrate and mediator for exo-electrogens, and the maximum power density of 10.0 W/m3 with 82.4% removal of chemical oxygen demand was achieved while treating synthetic wastewater. Therefore, this cost-effective exploration demonstrated successful bioelectricity production and concomitant wastewater treatment with solvent-free direct lipid recovery from wet algal biomass through PMFC.

Waste-derived iron catalyzed bio-electro-Fenton process for the cathodic degradation of surfactants.

The application of waste-derived iron for reuse in wastewater treatment is an effective way of utilizing waste and attaining sustainability in the overall process. In the present investigation, bio-electro-Fenton process was initiated for the cathodic degradation of surfactants using waste-iron catalyzed MFC (WFe-MFC). The waste-iron was derived from spent tonner ink using calcination at 600 °C. Three surfactants namely, sodium dodecyl sulphate (SDS), cetyltrimethylammonium bromide, and Triton x-100 were selected as target pollutants. The effect of experimental factors like application of catalyst, contact time, external resistance, and anodic substrate concentration on the SDS degradation was investigated. At a neutral pH, the cathodic surfactants removal efficiency in WFe-MFC was above 85% in a contact time of 180 min with the initial surfactant concentration of ∼20 mg L-1 and external resistance of 100 Ω. The long-term operation using secondary treated real wastewater with unchanged cathode proved that the catalyst was still active to produce effluent SDS concentration of less than 1 mg L-1 in 4 h of contact time after 16 cycles. In a way, the present investigation suggests a potential application for spent tonner ink in the form of Fenton catalyst for wastewater treatment via bio-electro-Fenton MFC.

Optimum dose of Chaetoceros for controlling methanogenesis to improve power production of microbial fuel cell.

The marine algae Chaetoceros contains hexadecatrienoic acid, which suppresses methanogen development and improves the coulombic efficiency (CE) of microbial fuel cells (MFC). To inhibit the methanogens, optimum concentration of marine algae should be added to the anaerobic sludge to enhance the performance of MFC. A varying concentration of Chaetoceros ranging from 1 to 20 mg/mL was carried out for pretreatment of an anaerobic-mix consortium to suppress methanogens. MFC inoculated with pretreated anaerobic sludge with 10 mg/mL Chaetoceros showed a maximum power density of 21.62 W/m3 and a maximum CE of 37.25%, which was considerably higher than the treatment with other concentrations. At 10 mg/mL concentration, Tafel analysis of the anode in the MFC showed a higher exchange current density of 66.35 mA/m2 and a lower charge transfer resistance of 0.97 Ω.m2, revealing higher bio-electrochemical activity. The performance of MFC improved when the concentration of Chaetoceros was increased up to 10 mg/mL, but then began to decline as the concentration increased further. Thus, the optimum dose of Chaetoceros to be added in the mix-anaerobic consortium to optimize the power performance of MFC was determined, which can be carried out in scaled-up MFCs.

Microbial fuel cell coupled Fenton oxidation for the cathodic degradation of emerging contaminants from wastewater: Applications and challenges.

Urbanization and industrialization have resulted in the escalation of the occurrence of emerging contaminants (EC) in the wastewater and ultimately to the receiving water bodies due to their bio-refractory nature. The presence of ECs in the water bodies adversely affects all three domains of life, viz. bacteria, archaea and eukaryotes, and eventually the ecosystem. Fenton oxidation is one of the most suitable method that is capable of degrading a variety of ECs by employing a strong oxidizing agent in the form of •OH. The coupling of Fenton oxidation with microbial fuel cell (MFC) offers benefits, such as low-cost, minimal requirement of external energy, and in-situ generation of oxidizing agents. The resulting system, termed as bio-electro-Fenton MFC (BEF-MFC), is capable of degrading the ECs in the cathodic chamber, while harvesting bioelectricity and simultaneously removing oxidizable organic matter from wastewater in the anodic chamber. This review discusses the applications of BEF-MFC for the treatment of dyes, pharmaceuticals, pesticides, and real complex wastewaters. Additionally, the effect of operating conditions on the performance of BEF-MFC are elaborated and emphasis is also given on possible future direction of research that can be adopted in BEF-MFC in the purview of up-scaling.

Bacterial signalling mechanism: An innovative microbial intervention with multifaceted applications in microbial electrochemical technologies: A review.

Microbial electrochemical technologies (METs) are a set of inventive tools that generate value-added by-products with concomitant wastewater remediation. However, due to the bottlenecks, like higher fabrication cost and inferior yield of resources, these inventive METs are still devoid of successful field-scale implementation. In this regard, application of quorum sensing (QS) mechanism to improve the power generation of the METs has gained adequate attention. The QS is an intercellular signalling mechanism that controls the bacterial social network in its vicinity via the synthesis of diffusible signal molecules labelled as auto inducers, thus ameliorating yield of valuables produced through METs. This state-of-the-art review elucidates different types of QS molecules and their working mechanism with the special focus on the widespread application of QS in the field of METs for their performance enhancement. Thus, this review intends to guide the researchers in rendering scalability to METs by integrating innovative QS mechanisms into them.

Live diatoms as potential biocatalyst in a microbial fuel cell for harvesting continuous diafuel, carotenoids and bioelectricity.

Microbial fuel cell (MFC) with live diatoms (Nitzschia palea) displacing bacteria in the anodic chamber generated electrical potential. Unlike other microalgae, diatoms fix 25% of atmospheric CO2, thus releasing O2. They perform photolysis of water by photosynthesis in the plastid during light photoperiod and cellular respiration in the mitochondria during dark, producing electrons and protons, respectively. The electrogenic property of diatom was explored and evaluated by comparing the potential changes with reference fuel cell without diatoms and that operated with diatoms in the anodic chamber. Such photosynthetic diatom microbial fuel cell (PDMFC) employed f/2 media rich in nitrates, phosphates, metasilicates, trace metals and vitamins as the anolyte and potassium permanganate as catholyte enhanced the output voltage by 3rd day. The maximum power density for PDMFC was 12.62 mWm-2 and coulombic efficiency of 22.95%. Besides this, the fixed diatom cells at anode showed about 64.28% increase in lipid production on 15th day compared to that on 1st day along with the increment in formation of complex fatty acid methyl esters and carotenoids during its operation. Hence, diatoms can be envisaged to substitute bacteria in the anodic chamber of MFC to simultaneously produce bioelectricity and other valuable compounds. Further their silica nanoporous architecture serve as good absorbents for heavy metal removal found in many wastewaters.

A novel bio-electro-Fenton process for eliminating sodium dodecyl sulphate from wastewater using dual chamber microbial fuel cell.

The frequent occurrence of surfactants in urban wastewaters represents a multifaceted environmental concern. In this investigation, bio-electro-Fenton-microbial fuel cell (BEF-MFC) was developed for the degradation of sodium dodecyl sulphate (SDS) from wastewater. The synthesised cathode catalyst (powdered activated carbon and iron oxide) facilitated the Fenton reaction in the cathodic chamber of the MFC, concurrently generating a maximum power density of 105.67 mW m-2. The overall performance of the BEF-MFC for SDS removal and power generation excelled the control MFC (C-MFC) having carbon black coated cathode under similar operating conditions. Although, the rate of SDS degradation was favourable in acidic pH, under neutral pH, 70.8 ± 6.4% of SDS degradation was achieved in 120 min in BEF-MFC. A comparison of environmental impacts of BEF-MFC with up-flow MFC and electrochemical oxidation using life cycle assessment tool suggests that BEF-MFC can be one of the promising technologies for the tertiary treatment of wastewater.

High throughput techniques for the rapid identification of electroactive microorganisms.

Electroactive microorganisms (EAM), capable of executing extracellular electron transfer (EET) in/out of a cell, are employed in microbial electrochemical technologies (MET) and bioelectronics for harnessing electricity from wastewater, bioremediation and as biosensors. Thus, investigation on EAM is becoming a topic of interest for multidisciplinary areas, such as environmental science, energy and health sectors. Though, EAM are widespread in three domains of life, nevertheless, only a few hundred EAM have been identified so far and hence, the rapid identification of EAM is imperative. In this review, the techniques that are developed for the direct identification of EAM, such as azo dye and WO3 based techniques, dielectrophoresis, potentiostatic/galvanometric techniques, and other indirect methods, such as spectroscopy and molecular biology techniques, are highlighted with a special focus on time required for the detection of these EAM. The bottlenecks for identifying EAM and the knowledge gaps based on the present investigations are also discussed. Thus, this review is intended to encourage researchers for devolving high-throughput techniques for identifying EAM with more accuracy, while consuming less time.

Methanogenesis inhibitors used in bio-electrochemical systems: A review revealing reality to decide future direction and applications.

Microbial fuel cell (MFC) is a robust technology capable of treating real wastewaters by utilizing mixed anaerobic microbiota as inoculum for producing electricity from oxidation of the biodegradable matters. However, these mixed microbiota comprises of both electroactive microorganisms (EAM) and substrate/electron scavenging microorganisms such as methanogens. Hence, in order to maximize bioelectricity from MFC, different physio-chemical techniques have been applied in past investigations to suppress activity of methanogens. Interestingly, recent investigations exhibit that methanogens can produce electricity in MFC and possess the cellular machinery like cytochrome c and Type IV pili to perform extracellular electron transfer (EET) in the presence of suitable electron acceptors. Hence, in this review, in-depth analysis of versatile behaviour of methanogens in both MFC and natural anaerobic conditions with different inhibition techniques is explored. This review also discusses the future research directions based on the latest scientific evidence on role of methanogens for EET in MFC.

Enhancing the Performance of Microbial Fuel Cell by Using Chloroform Pre-treated Mixed Anaerobic Sludge to Control Methanogenesis in Anodic Chamber.

Formation of methane in the anodic chamber of a microbial fuel cell (MFC) indicates an energy inefficiency in electricity generation as the energy required for electrogenesis gets redirected to methanogenesis. The hypothesis of this research is that inhibition of methanogenesis in the mixed anaerobic anodic inoculum is associated with an enhanced activity of the electrogenic bacterial consortia. Hence, the primary objective of this investigation is to evaluate the ability of chloroform to inhibit the methanogenesis at different dosing to enhance the activity of electrogenic consortia in MFC. A higher methane inhibition and hence an enhanced performance of MFC was achieved when mixed anaerobic sludge, collected from septic tank, was used as inoculum after pre-treatment with 0.25% (v/v) chloroform dosing (MFC-0.25CF). The MFC-0.25CF attained a maximum power density of 8.51 W/m3, which was more than twice as that of MFC inoculated with untreated sludge. Also, a clear correlation between the chloroform dosing, methane inhibition, wastewater treatment, and power generation was established, which demonstrated the effectiveness of the technique in enhancing power generation in MFC along with adequate biodegradation of organic matter present in wastewater at an optimum chloroform dosing of 0.25% (v/v) to inhibit methanogenesis.

Plant secondary metabolites induced electron flux in microbial fuel cell: investigation from laboratory-to-field scale.

Wastewater treatment coupled with electricity recovery in microbial fuel cell (MFC) prefer mixed anaerobic sludge as inoculum in anodic chamber than pure stain of electroactive bacteria (EAB), due to robustness and syntrophic association. Genetic modification is difficult to adopt for mixed sludge microbes for enhancing power production of MFC. Hence, we demonstrated use of eco-friendly plant secondary metabolites (PSM) with sub-lethal concentrations to enhance the rate of extracellular electron transfer between EAB and anode and validated it in both bench-scale as well as pilot-scale MFCs. The PSMs contain tannin, saponin and essential oils, which are having electron shuttling properties and their addition to microbes can cause alteration in cell morphology, electroactive behaviour and shifting in microbial population dynamics depending upon concentrations and types of PSM used. Improvement of 2.1-times and 3.8-times in power densities was observed in two different MFCs inoculated with Eucalyptus-extract pre-treated mixed anaerobic sludge and pure culture of Pseudomonas aeruginosa, respectively, as compared to respective control MFCs operated without adding Eucalyptus-extract to inoculum. When Eucalyptus-extract-dose was spiked to anodic chamber (125 l) of pilot-scale MFC, treating septage, the current production was dramatically improved. Thus, PSM-dosing to inoculum holds exciting promise for increasing electricity production of field-scale MFCs.

Improved Performance of Microbial Fuel Cell by In Situ Methanogenesis Suppression While Treating Fish Market Wastewater.

The fish market wastewater, which is rich in ammonium concentration, was investigated to explore its ability of in situ suppression of methanogenesis in the anodic chamber of microbial fuel cell (MFC) while treating it and to ensure non-reoccurrence of methanogenic consortia in the anodic chamber during its long-term operations. A lower specific methanogenic activity (0.097g chemical oxygen demand (COD)CH4/g volatile suspended solids (VSS). day) with a higher power density (3.81 ± 0.19 W/m3) was exhibited by the MFC operated with raw fish market wastewater as compared to the MFC fed with synthetic wastewater (0.219g CODCH4/g VSS. day and 1.75 ± 0.09 W/m3, respectively). The enhanced electrochemical activity of anodic biofilm of MFC fed with raw fish market wastewater than the MFC fed with synthetic wastewater further advocated the enhanced electrogenic activity and suppression of methanogenesis, because of the presence of higher ammonium content in the feed. This, in response, reduced the internal resistance (55 Ω), enhanced the coulombic efficiency (21.9 ± 0.3%) and normalized the energy recovery (0.27 kWh/m3) from the MFC fed with fish market wastewater than the MFC fed with synthetic wastewater (92 Ω, 15.7 ± 0.3% and 0.13 kWh/m3, respectively). Thus, while treating the fish market wastewater in the anodic chamber of MFC, any costly and repetitive treatment procedures for anodic microorganisms are not required for suppression of methanogens to ensure higher activity of electrogenic bacteria for higher electricity harvesting.

Surfactant removal from wastewater using photo-cathode microbial fuel cell and laterite-based hybrid treatment system.

Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant, which finds its way to the receiving water body due to the incapability of conventional wastewater treatment systems to completely remove it. A hybrid treatment system consisting of upflow microbial fuel cell (MFC) with titanium dioxide (TiO2) as a photocathode catalyst was developed for treating synthetic wastewater spiked with SDS (10.00 ± 0.46 mg L-1). Effluent from anodic chamber of MFC was passed through raw laterite soil filter followed by the photo-cathodic chamber with TiO2-coated cathode irradiated with the UV spectrum. This hybrid system was operated under varying hydraulic retention time (HRT) in anodic chamber of MFC. The SDS removal efficiency of more than 96% along with organic matter removal efficiency of more than 71% was obtained by this hybrid system at different HRTs. The MFC having cathode coated with TiO2 could generate a maximum power density of 0.73 W m-3 and 0.46 W m-3 at the HRT of 12 h and 8 h, respectively, showing the adverse effect of increased SDS loading rate on the electrical performance of MFC. This investigation highlighted the importance of HRT in anodic chamber of MFC and offered solution for effective removal of surfactant from wastewater.

Waste-derived biochar: Applications and future perspective in microbial fuel cells.

Application of microbial fuel cell (MFC) is coming to the forefront as a dual-purpose system for wastewater treatment and energy recovery. Future research should emphasize on developing low-cost field-scale MFCs for removal of organic matter, nutrients, xenobiotic and recalcitrant compounds from wastewaters and powering low energy devices. For achieving this, low-cost electrodes, low-cost yet efficient cathode catalysts and proton exchange membrane (PEM) should be developed from waste-based resources to salvage the waste-derived material as much as possible, thereby reducing the fabrication cost of this device. Biochar is one such low-cost material, which has wide range of applications. This review discusses different applications of biochar in MFC, viz. in the form of standalone electrodes, electrocatalyst and material for PEM in light of different characteristics of biochar. Further emphasis is given on the future direction of research for implementation of biochar-based PEMs and electrodes in field-scale MFCs.

Utilisation of waste medicine wrappers as an efficient low-cost electrode material for microbial fuel cell.

Waste generation from healthcare facilities now has become a concerning issue as it contain plastic and metals. Medicine wrappers are one of the major portions of healthcare solid waste, which impel intensive solid waste management practice due to fewer possibilities of deriving by-products. However, it can be recycled and used as an electrode material in microbial fuel cells (MFCs). An electrode material for application in MFCs is a crucial component, which governs total fabrication cost as well as power recovery, thus a cost-effective, stable and durable electrode is essential. In this endeavour, a new metallic (aluminium) waste material, a waste medicine wrapper (WMW), was evaluated for feasibility to be used as anode/cathode in MFCs. Based on the stability test under corrosive environment (1 N KCl), the WMW electrode sustained a maximum current of 46 mA during cyclic voltammetry (CV) and noted only 14% reduction in current at an applied voltage of +0.4 V after 2500 s in chronoamperometry, indicating its good stability. Power recovery from MFC using WMW was higher than the MFC using bare carbon felt as an anode (27 vs. 21 mW/m2). The entire analytical test results viz. CV, electrochemical impedance spectroscopy and power performance established WMW as an excellent anode rather than cathode material.