Long term declines in the functional diversity of sharks in the coastal oceans of eastern Australia.

Human impacts lead to widespread changes in the abundance, diversity and traits of shark assemblages, altering the functioning of coastal ecosystems. The functional consequences of shark declines are often poorly understood due to the absence of empirical data describing long-term change. We use data from the Queensland Shark Control Program in eastern Australia, which has deployed mesh nets and baited hooks across 80 beaches using standardised methodologies since 1962. We illustrate consistent declines in shark functional richness quantified using both ecological (e.g., feeding, habitat and movement) and morphological (e.g., size, morphology) traits, and this corresponds with declining ecological functioning. We demonstrate a community shift from targeted apex sharks to a greater functional richness of non-target species. Declines in apex shark functional richness and corresponding changes in non-target species may lead to an anthropogenically induced trophic cascade. We suggest that repairing diminished shark populations is crucial for the stability of coastal ecosystems.

Turning a lost reef ecosystem into a national restoration program.

Achieving a sustainable socioecological future now requires large-scale environmental repair across legislative borders. Yet, enabling large-scale conservation is complicated by policy-making processes that are disconnected from socioeconomic interests, multiple sources of knowledge, and differing applications of policy. We considered how a multidisciplinary approach to marine habitat restoration generated the scientific evidence base, community support, and funding needed to begin the restoration of a forgotten, functionally extinct shellfish reef ecosystem. The key actors came together as a multidisciplinary community of researchers, conservation practitioners, recreational fisher communities, and government bodies that collaborated across sectors to rediscover Australia's lost shellfish reefs and communicate the value of its restoration. Actions undertaken to build a case for large-scale marine restoration included synthesizing current knowledge on Australian shellfish reefs and their historical decline, using this history to tell a compelling story to spark public and political interest, integrating restoration into government policy, and rallying local support through community engagement. Clearly articulating the social, economic, and environmental business case for restoration led to state and national funding for reef restoration to meet diverse sustainability goals (e.g., enhanced biodiversity and fisheries productivity) and socioeconomic goals (e.g., job creation and recreational opportunities). A key lesson learned was the importance of aligning project goals with public and industry interests so that projects could address multiple political obligations. This process culminated in Australia's largest marine restoration initiative and shows that solutions for large-scale ecosystem repair can rapidly occur when socially valued science acts on political opportunities.

Transformación de un Ecosistema Arrecifal Perdido en un Programa Nacional de Restauración Resumen Actualmente se requiere una reparación ambiental a gran escala que atraviese fronteras legislativas para lograr un futuro socio-ecológico sustentable. Aun así, habilitar la conservación a gran escala es complicado debido a los procesos de elaboración de políticas que están desconectadas de los intereses socio-económicos, las múltiples fuentes de conocimiento y las diferentes aplicaciones de las políticas. Consideramos cómo una estrategia multidisciplinaria para la restauración de hábitats marinos generó una base de evidencia científica, apoyo comunitario y el financiamiento necesario para así iniciar la restauración de un ecosistema arrecifal de conchas funcionalmente extinto. Los actores clave formaron una comunidad multidisciplinaria de investigadores, practicantes de la conservación, comunidades de pescadores recreativos y órganos gubernamentales que colaboró con varios sectores para redescubrir los arrecifes perdidos de Australia y comunicó el valor de su restauración. Las acciones realizadas para armar el caso para la restauración marina a gran escala incluyeron la síntesis del conocimiento actual sobre los arrecifes de conchas en Australia y su declinación histórica, el uso de esta historia para contar una narración convincente que active el interés público y político, la integración de la restauración a la política gubernamental y la movilización del apoyo local por medio de la participación comunitaria. Claramente, la articulación del caso del negocio social, económico y ambiental para la restauración llevó al financiamiento estatal y nacional para la restauración arrecifal a cumplir diversos objetivos socio-económicos (p. ej.: creación de empleos, oportunidades recreativas) y de restauración (p. ej.: una productividad realzada de la biodiversidad y las pesquerías). Una lección clave que aprendimos fue lo importante que es alinear los objetivos del proyecto con los intereses públicos y de la industria, de tal manera que los proyectos aborden las múltiples obligaciones políticas. Este proceso culminó con la iniciativa de restauración marina más grande en Australia y demuestra que las soluciones para la reparación de los ecosistemas a gran escala pueden ocurrir rápidamente cuando la ciencia con valor social actúa sobre las oportunidades políticas.

Neonatal subchronic toxicity and in vitro genotoxicity studies of the human-identical milk oligosaccharide 3-fucosyllactose.

Human milk oligosaccharides, such as 3-fucosyllactose (3-FL), are bioactive components of breast milk associated with benefits for infant growth and development. Structurally identical compounds (human-identical milk oligosaccharides-HiMOs) can be produced using microbial fermentation, allowing their use in infant formula to increase its similarity with human milk. Toxicological studies are required to demonstrate safety of HiMOs and that of any impurities potentially carried over from the manufacturing process. Biotechnologically produced 3-FL was tested for potential genotoxicity (bacterial reverse mutation test and in vitro mammalian micronucleus test) and subchronic toxicity (90-day study with neonatal rats). In the 90-day study, 3-FL was administered by gavage to rats once daily from Day 7 of age, at doses up to 4000 mg/kg body weight (bw)/day (the maximum feasible dose), followed by a 4-week recovery period. Reference controls received 4000 mg/kg bw/day of oligofructose, an ingredient permitted for use in infant formula. Results for the genotoxicity studies were negative. In the 90-day study, there were no adverse effects of 3-FL on any of the parameters measured; thus, the no-observed-adverse-effect level (NOAEL) was 4000 mg/kg bw/day (the highest dose tested). These results support the safety of biotechnologically produced 3-FL for use in infant formula and other foods.

Dredging transforms the seafloor and enhances functional diversity in urban seascapes.

Landscape modification alters the condition of ecosystems and the complexity of terrain, with consequences for animal assemblages and ecosystem functioning. In coastal seascapes, dredging is routine practice for extracting sediments and maintaining navigation channels worldwide. Dredging modifies processes and assemblages by favouring species with wide trophic niches, diverse habitat requirements and tolerances to dredge-related eutrophication and sedimentation. Dredging also transforms the three-dimensional features of the seafloor, but the functional consequences of these terrain changes remain unclear. We investigated the effects of terrain modification on the functional diversity of fish assemblages in natural and dredged estuaries to examine whether dredging programs could be optimised to minimise impacts on ecological functioning. Fish assemblages were surveyed with baited remote underwater video stations and variation in functional niche space was described using species traits to calculate metrics that index functional diversity. Terrain variation was quantified with nine complementary surface metrics including depth, aspect, curvature, slope and roughness extracted from sonar-derived bathymetry maps. Functional diversity was, surprisingly, higher in dredged estuaries, which supported more generalist species with wider functional niches, and from lower trophic levels, than natural estuaries. These positive effects of dredging on functional diversity were, however, spatially restricted and were linked to both the area and orientation of terrain modification. Functional diversity was highest in urban estuaries where dredged channels were small (i.e. <1% of the estuary), and where channel slopes were orientated towards the poles (i.e. 171-189°), promoting both terrain variation and light penetration in urban estuaries. Our findings highlight previously unrecognised functional consequences of terrain modification that can easily be incorporated into dredging programs. We demonstrate that restricting the spatial extent of dredging operations and the orientation of dredged channel slopes, wherever this is practical, could help to limit impacts on ecosystem functioning and productivity in urban seascapes.

Human modifications to estuaries correlate with the morphology and functional roles of coastal fish.

Animals can respond to human impacts by favouring different morphological traits or by exploiting different food resources. We quantified the morphology and diet of four functionally different fish species (n = 543 fish) from 13 estuaries with varying degrees of human modification in Queensland, Australia. We found differences in the responses of trophic groups to the environmental conditions of estuaries; principally the extent of seagrass in the estuary, and the amount of shoreline and catchment urbanisation. Here, seagrass and urbanisation extent correlated with the diet and morphology of zooplanktivores and detritivores; thereby indicating that human modifications may modify these species functional roles. Conversely, environmental variables did not correlate with the diet or morphology of zoobenthivores or piscivores thereby indicating that human modifications may have less an effect on these species functional roles. Our findings demonstrate that anthropogenic impacts to coastal ecosystems might extend from the traditionally measured metrics of abundance and diversity.

Genotoxicity and neonatal subchronic toxicity assessment of a novel mixture of the human-identical milk oligosaccharides lacto-N-fucopentaose I and 2'-fucosyllactose.

Human milk oligosaccharides (HMOs) are a complex group of bioactive molecules largely observed in human breast milk but also occurring in limited amounts in other mammalian milks. Advances in biotechnology have enabled production of human-identical milk oligosaccharides (HiMOs), structurally identical molecules to HMOs found naturally in human milk, intended for addition to infant formula to more closely replicate breast milk. Biosynthesis of a novel mixture of two major HMOs, lacto-N-fucopentaose I and 2'-fucosyllactose (LNFP-I/2'-FL), recently became possible. To support the safety of LNFP-I/2'-FL for use in infant formula and other foods, it was subject to a safety assessment comprising a bacterial reverse mutation test, an in vitro mammalian cell micronucleus test, and a 90-day oral gavage study in neonatal rats. In the 90-day study (the first HiMO study to include the new endocrine-sensitive endpoints described in the 2018 version of OECD Test Guideline 408), LNFP-I/2'-FL was administered by oral gavage to neonatal rats once daily (from Day 7 of age) for 90 consecutive days, at doses up to 5000 mg/kg bw/day, followed by a 4-week recovery period. Concurrent reference controls received 5000 mg/kg bw/day of the approved infant formula ingredient oligofructose. LNFP-I/2'-FL was nongenotoxic in vitro. The highest dose tested (5000 mg/kg bw/day) was established as the no-observed-adverse-effect level in the 90-day study, as there were no test article-related adverse effects on clinical observations, body weight, food consumption, clinical pathology, and organ weights nor any noteworthy macroscopic or microscopic findings. This supports the safety of LNFP-I/2'-FL for its intended uses in food.

Diverse land uses and high coastal urbanisation do not always result in harmful environmental pollutants in fisheries species.

Human activities in coastal catchments can cause the accumulation of pollutants in seafood. We quantified the concentration of heavy metals, pesticides and PFASs in the flesh of the fisheries species yellowfin bream Acanthopagrus australis (n = 57) and mud crab Scylla serrata (n = 65) from 13 estuaries in southeast Queensland, Australia; a region with a variety of human land uses. Pollutants in yellowfin bream were best explained by the extent of intensive uses in the catchment. Pollutants in mud crabs were best explained by the extent of irrigated agriculture and water bodies. No samples contained detectable levels of pesticides, and only six samples contained low levels of PFASs. Metals were common in fish and crab flesh, but only mercury in yellowfin bream from the Mooloolah River breached Australian food safety standards. High pollutant presence and concentration is not the norm in seafood collected during routine surveys, even in estuaries with highly modified catchments.

Landscape context and nutrients modify the effects of coastal urbanisation.

Estuaries are focal points for coastal cities worldwide, their habitats frequently transformed into engineered shorelines abutting waters with elevated nutrients in an urbanised landscape. Here we test for relationships between shoreline armouring and nutrients on the diversity and trophic composition of fish assemblages across 22 estuaries in eastern Australia. Urbanisation was associated with fish diversity and abundance, but there were differences in the effects of shoreline armouring and nutrient level on the trophic composition of fish assemblages. Fish diversity and the abundance of most trophic groups, particularly omnivores, zoobenthivores and detritivores, was greatest in highly urban estuaries. We show that estuarine fish assemblages are associated with urbanisation in more nuanced ways than simple habitat transformation would suggest, but this depends on the broader environmental context. Our findings have wider implications for estuarine conservation and restoration, emphasizing that ecological benefits of habitat measures may depend on both landscape attributes and water quality in urban settings.

Low redundancy and complementarity shape ecosystem functioning in a low-diversity ecosystem.

Ecosystem functioning is positively linked to biodiversity on land and in the sea. In high-diversity systems (e.g. coral reefs), species coexist by sharing resources and providing similar functions at different temporal or spatial scales. How species combine to deliver the ecological function they provide is pivotal for maintaining the structure, functioning and resilience of some ecosystems, but the significance of this is rarely examined in low-diversity systems such as estuaries. We tested whether an ecological function is shaped by biodiversity in a low-diversity ecosystem by measuring the consumption of carrion by estuarine scavengers. Carrion (e.g. decaying animal flesh) is opportunistically fed on by a large number of species across numerous ecosystems. Estuaries were chosen as the model system because carrion consumption is a pivotal ecological function in coastal seascapes, and estuaries are thought to support diverse scavenger assemblages, which are modified by changes in water quality and the urbanization of estuarine shorelines. We used baited underwater video arrays to record scavengers and measure the rate at which carrion was consumed by fish in 39 estuaries across 1,000 km of coastline in eastern Australia. Carrion consumption was positively correlated with the abundance of only one species, yellowfin bream Acanthopagrus australis, which consumed 58% of all deployed carrion. The consumption of carrion by yellowfin bream was greatest in urban estuaries with moderately hardened shorelines (20%-60%) and relatively large subtidal rock bars (>0.1 km2 ). Our findings demonstrate that an ecological function can be maintained across estuarine seascapes despite both limited redundancy (i.e. dominated by one species) and complementarity (i.e. there is no spatial context where the function is delivered significantly when yellowfin bream are not present) in the functional traits of animal assemblages. The continued functioning of estuaries, and other low-diversity ecosystems, might therefore not be tightly linked to biodiversity, and we suggest that the preservation of functionally dominant species that maintain functions in these systems could help to improve conservation outcomes for coastal seascapes.

Toxicological safety evaluation of the human-identical milk oligosaccharide 6'-sialyllactose sodium salt.

Human milk oligosaccharides (HMOs) are abundant in breastmilk, but their presence in infant formula is negligible. Sialylated HMOs, such as 6'-sialyllactose, constitute a significant portion of the HMO fraction of human milk and are linked to important biological functions. To produce infant formula that is more comparable with human milk, biosynthesized sialyllactoses known as human-identical milk oligosaccharides (structurally identical counterparts to their respective naturally occurring HMOs in breastmilk) are proposed for use in infant formula and other functional foods for the general population. To support the safety of 6'-sialyllactose sodium salt (6'-SL), a 90-day oral (gavage) toxicity study and in vitro genotoxicity tests were conducted. The 90-day study is the first to be conducted with 6'-SL using neonatal rats (day 7 of age at the start of dosing), thus addressing safety of 6'-SL for consumption by the most sensitive age group (infants). In the 90-day study, neonatal rats received 6'-SL at doses up to 5000 mg/kg body weight (BW)/day and reference controls received 5000 mg/kg BW/day of fructooligosaccharide (an ingredient approved for use in infant formula) for comparison with the high-dose 6'-SL group, followed by a 4-week recovery period. There was no evidence of genotoxicity in vitro. No test item-related adverse effects were observed on any parameter in the 90-day study, thus the high dose (5000 mg/kg BW/day) was established as the no-observed-adverse-effect level. These results confirm that 6'-SL is safe for use in formula milk for infants and in other functional foods for the general population.

Toxicological safety assessment of the human-identical milk oligosaccharide 3'-sialyllactose sodium salt.

Human breastmilk is a mixture of nutrients, hormones and bioactive molecules that are vital for infant growth and development. Infant formula (IF) lacks many of these compounds, most notably human milk oligosaccharides (HMOs), which are abundant in breastmilk but scarce in IF. Sialyllactoses, such as 3'-sialyllactose, constitute a large portion of the HMO fraction. To produce IF that matches breastmilk more closely, biosynthesized human-identical milk oligosaccharides (structurally identical to HMOs) such as 3'-sialyllactose sodium salt (3'-SL) are proposed for use in IF and foods for the general population. The safety assessment of 3'-SL comprised in vitro genotoxicity tests and a 90-day oral (gavage) toxicity study. This is the first 90-day study conducted with 3'-SL using neonatal rats (7 days old at the start of dosing-equivalent age to newborn human infants in terms of central nervous system and reproductive development), demonstrating the safety of 3'-SL for consumption by infants, the most sensitive age group. The neonatal rats received 3'-SL at doses up to 5,000 mg/kg body weight (BW)/day and reference controls received 5,000 mg/kg BW/day of fructooligosaccharide (an ingredient approved for use in IF) for comparison with the high-dose 3'-SL group, followed by a 4-week recovery period. There was no evidence of genotoxicity in vitro. In the absence of any test item-related adverse effects in the 90-day study, the high dose (5,000 mg/kg BW/day) was established as the no-observed-adverse-effect level. This confirms the safety of 3'-SL for use in IF for infants, as well as in functional foods for the general population.

Analysis of historical negative control group data from the in vitro micronucleus assay using human lymphocytes.

A database of the micronuclei counts was built up for historical negative control data from human lymphocyte in vitro micronuclei tests (MnVit) carried out in 8 laboratories with experience of the method. The mean incidence of micronucleated cells (mnt)/1000 cells ranged from 2.2/1000 to 15.9/1000. There were no large differences in incidence between the presence or absence of S9 mix or between different treatment lengths. There was also little evidence that different solvents affected the numbers of micronuclei appreciably. A number of laboratories did show significant inter-experiment variability, indicating that there remained unidentified factors affecting frequencies. Donor variance may be one such factor. Inter-individual variability may explain some of these differences. The approximate 7.5-fold difference in mnt/1000 scores in a relatively small group of experienced laboratories illustrates the potential complications that can arise if a metric like a fold increase was considered the only biologically important finding. Although there is inherent variability between experiments, it was evident that within a laboratory the overall laboratory mean remains constant over time. It is believed that these findings will provide help to laboratories conducting studies using human lymphocytes in the MnVit and to those involved in the assessment of MnVit results.

Optimizing conservation benefits for threatened beach fauna following severe natural disturbances.

Wildlife on sandy beaches is often constrained by transformation of natural areas for human use, and opportunities for acquiring or restoring new habitat are rare. Storms can often force changes in land use naturally by re-shaping coastal landforms, thereby creating high quality habitat; yet, wildlife requirements are seldom considered in post-storm recovery planning, and conservation practitioners lack suitable evidence to argue for the protection of habitats freshly formed by storms. Here we used a maximum-likelihood spatial modeling approach to quantify impacts of Hurricane Sandy (mid-Atlantic United States, October 2012) on nesting habitat of four bird species of conservation concern: American oystercatchers, black skimmers, least terns and piping plovers. We calculated the immediate storm-created changes (loss, persisting, gained) in nesting habitat under two levels of conservation protections: the current regulatory framework, and a scenario in which all potential habitats were under conservation protection. Hurricane Sandy resulted in apparent large gains for least terns (+181 ha) and piping plovers (+289 ha). However, actual gains were reduced to 16 ha for plovers and reversed for least terns (net loss of 6.4 ha) because newly formed habitat occurred outside existing reserve boundaries. Similarly, under the current management framework, black skimmer nesting habitat decreased by ~164 ha. We also tested whether birds benefited from newly created nesting habitat by identifying nest and colony locations for three years following Hurricane Sandy. All species overwhelmingly nested in habitat that existed prior the storm (76-98% of all nests/colonies); only a small percentage (≤17% for all species) occupied newly created habitat. We conclude that static spatial conservation efforts fail to capitalize on potentially large gains resulting from storms for several species and recommend flexible spatial conservation investments as a key input in post-storm recovery planning.

Effects of seascape connectivity on reserve performance along exposed coastlines.

Seascape connectivity (landscape connectivity in the sea) can modify reserve performance in low-energy marine ecosystems (e.g., coral reefs, mangroves, and seagrass), but it is not clear whether similar spatial linkages also shape reserve effectiveness on high-energy, exposed coastlines. We used the surf zones of ocean beaches in eastern Australia as a model system to test how seascape connectivity and reserve attributes combine to shape conservation outcomes. Spatial patterns in fish assemblages were measured using baited remote underwater video stations in 12 marine reserves and 15 fished beaches across 2000 km of exposed coastline. Reserve performance was shaped by both the characteristics of reserves and the spatial properties of the coastal seascapes in which reserves were embedded. Number of fish species and abundance of harvested fishes were highest in surf-zone reserves that encompassed >1.5 km of the surf zone; were located < 100 m to rocky headlands; and included pocket beaches in a heterogeneous seascape. Conservation outcomes for exposed coastlines may, therefore, be enhanced by prioritizing sufficiently large areas of seascapes that are strongly linked to abutting complementary habitats. Our findings have broader implications for coastal conservation planning. Empirical data to describe how the ecological features of high-energy shorelines influence conservation outcomes are lacking, and we suggest that seascape connectivity may have similar ecological effects on reserve performance on both sheltered and exposed coastlines.

Efectos de la Conectividad de Paisajes Marinos sobre el Desempeño de las Reservas a lo largo de Costas Expuestas Resumen La conectividad entre paisajes marinos puede modificar el desempeño de las reservas en los ecosistemas marinos de baja energía (p. ej.: arrecifes de coral, manglares, pastos marinos), pero no está claro si las conexiones espaciales similares también moldean la efectividad de las reservas en costas expuestas con alta energía. Usamos las zonas de rompimiento de las playas oceánicas en el este de Australia como sistema modelo para probar cómo la conectividad entre paisajes marinos y los atributos de la reserva se combinan para moldear los resultados de la conservación. Los patrones espaciales en los ensamblados de peces se midieron con estaciones remotas de video subacuático con carnada en doce reservas marinas y 15 playas a lo largo de 2000 km de costas expuestas. El desempeño de las reservas estuvo moldeado por las características de las reservas y las propiedades espaciales de los paisajes costeros en los cuales estaban insertadas las reservas. El número de especies de peces y la abundancia de peces recolectados fue mucho mayor en las reservas en las zonas de rompimiento que abarcaban >1.5 km de la zona de rompimiento; estaban localizadas a <100 m de cabos rocosos; e incluían playas pequeñas entre los cabos en un paisaje marino heterogéneo. Los resultados de conservación para las costas expuestas pueden, por lo tanto, mejorarse con la priorización suficiente de grandes áreas de paisajes marinos que están conectados fuertemente con hábitats complementarios colindantes. Nuestros hallazgos tienen consecuencias más generales para la planeación de la conservación costera. Los datos empíricos para describir cómo las características ecológicas de las costas con alta energía influyen sobre los resultados de conservación son muy pocos, y sugerimos que la conectividad entre paisajes marinos puede tener efectos ecológicos similares sobre el desempeño de las reservas en costas expuestas y resguardadas.

Preclinical safety evaluation of the human-identical milk oligosaccharide lacto-N-tetraose.

Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk. To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts. As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted. In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period. An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group. LNT was non-genotoxic in the in vitro tests. There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level. These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.

Safety evaluation of a mixture of the human-identical milk oligosaccharides 2'-fucosyllactose and difucosyllactose.

Human milk oligosaccharides (HMOs) are endogenous indigestible carbohydrates representing the largest compositional difference between human breastmilk and infant formula (IF). Two major HMOs in human breastmilk are 2'-fucosyllactose (2'-FL) and difucosyllactose (DFL); commercial IF can be supplemented with manufactured structurally identical versions of HMOs [known as human-identical milk oligosaccharides (HiMOs)] to better replicate the composition of human milk. As 2'-FL and DFL are always found together in human milk, a mixture of these HiMOs (2'-FL/DFL) has been proposed for use in IF and as a food ingredient. Safety assessment of 2'-FL/DFL included conduct of in vitro genotoxicity tests and a subchronic oral toxicity study. In the subchronic study, 2'-FL/DFL (8:1 ratio) was administered to neonatal rats at doses up to 5000 mg/kg body weight (bw)/day, once daily for 90 days, followed by a 4-week recovery period. A concurrent reference control group received 5000 mg/kg bw/day of an oligosaccharide already used in IF (fructooligosaccharide), for direct comparison with the high-dose 2'-FL/DFL group. No evidence of genotoxicity was observed. In the absence of compound-related adverse effects in the 90-day study, 5000 mg/kg bw/day was established as the no-observed-adverse-effect-level. These results support the use of 2'-FL/DFL in IF and as a food ingredient.

Functional plasticity in vertebrate scavenger assemblages in the presence of introduced competitors.

Introduced species may suppress or enhance ecological functions, or they may have neutral effects in ecosystems where they replace or complement native species. Few studies, however, have explicitly tested for these trajectories, and for the effect these might have for native species. In this study, we experimentally test the trajectory and scale of change in the function of 'carrion removal' at different carrion loads along ocean beaches in Eastern Australia that have different numbers of introduced red foxes (Vulpes vulpes) and several species of native raptors. We hypothesized that the 'positive' effect of foxes on carrion removal would be greatest at high carrion loads, because competition for resources between native and introduced species is lower. Scavenger abundance, fox occurrences, and carrion consumption by these species differed widely between locations and times. Despite distinct spatial differences in the structure of vertebrate scavenger assemblages, total carrion consumption was not significantly different between locations at any carrion load. This lack of variation in functional rates indicates potential functional plasticity in the scavenger assemblage and possible functional accommodation of red foxes. Neutral fox effects on ecological functions or the ecosystem more broadly are, however, very unlikely to extend beyond carrion consumption.

Marine turtles are not fussy nesters: a novel test of small-scale nest site selection using structure from motion beach terrain information.

BACKGROUND: Nest selection is widely regarded as a key process determining the fitness of individuals and viability of animal populations. For marine turtles that nest on beaches, this is particularly pivotal as the nesting environment can significantly control reproductive success.The aim of this study was to identify the environmental attributes of beaches (i.e., morphology, vegetation, urbanisation) that may be associated with successful oviposition in green and loggerhead turtle nests. METHODS: We quantified the proximity of turtle nests (and surrounding beach locations) to urban areas, measured their exposure to artificial light, and used ultra-high resolution (cm-scale) digital surface models derived from Structure-from-Motion (SfM) algorithms, to characterise geomorphic and vegetation features of beaches on the Sunshine Coast, eastern Australia. RESULTS: At small spatial scales (i.e., <100 m), we found no evidence that turtles selected nest sites based on a particular suite of environmental attributes (i.e., the attributes of nest sites were not consistently different from those of surrounding beach locations). Nest sites were, however, typically characterised by occurring close to vegetation, on parts of the shore where the beach- and dune-face was concave and not highly rugged, and in areas with moderate exposure to artificial light. CONCLUSION: This study used a novel empirical approach to identify the attributes of turtle nest sites from a broader 'envelope' of environmental nest traits, and is the first step towards optimizing conservation actions to mitigate, at the local scale, present and emerging human impacts on turtle nesting beaches.

Optimising Land-Sea Management for Inshore Coral Reefs.

Management authorities seldom have the capacity to comprehensively address the full suite of anthropogenic stressors, particularly in the coastal zone where numerous threats can act simultaneously to impact reefs and other ecosystems. This situation requires tools to prioritise management interventions that result in optimum ecological outcomes under a set of constraints. Here we develop one such tool, introducing a Bayesian Belief Network to model the ecological condition of inshore coral reefs in Moreton Bay (Australia) under a range of management actions. Empirical field data was used to model a suite of possible ecological responses of coral reef assemblages to five key management actions both in the sea (e.g. expansion of reserves, mangrove & seagrass restoration, fishing restrictions) and on land (e.g. lower inputs of sediment and sewage from treatment plants). Models show that expanding marine reserves (a 'marine action') and reducing sediment inputs from the catchments (a 'land action') were the most effective investments to achieve a better status of reefs in the Bay, with both having been included in >58% of scenarios with positive outcomes, and >98% of the most effective (5th percentile) scenarios. Heightened fishing restrictions, restoring habitats, and reducing nutrient discharges from wastewater treatment plants have additional, albeit smaller effects. There was no evidence that combining individual management actions would consistently produce sizeable synergistic until after maximum investment on both marine reserves (i.e. increasing reserve extent from 31 to 62% of reefs) and sediments (i.e. rehabilitating 6350 km of waterways within catchments to reduce sediment loads by 50%) were implemented. The method presented here provides a useful tool to prioritize environmental actions in situations where multiple competing management interventions exist for coral reefs and in other systems subjected to multiple stressor from the land and the sea.