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1.
Metallomics ; 12(4): 470-473, 2020 04 01.
Artículo en Inglés | MEDLINE | ID: mdl-32236192

RESUMEN

N-Truncated Aß4-42 displays a high binding affinity with CuII. A mechanistic scheme of the interactions between Aß4-42 and CuII has been proposed using a fluorescence approach. The timescales of different conversion steps were determined. This kinetic mechanism indicates the potential synaptic functions of Aß4-42 during neurotransmission.


Asunto(s)
Enfermedad de Alzheimer/metabolismo , Péptidos beta-Amiloides/metabolismo , Cobre/metabolismo , Fragmentos de Péptidos/metabolismo , Péptidos beta-Amiloides/química , Cobre/química , Cinética , Modelos Químicos , Estructura Molecular , Fragmentos de Péptidos/química , Unión Proteica , Termodinámica
2.
Biophys J ; 116(10): 1984-1993, 2019 05 21.
Artículo en Inglés | MEDLINE | ID: mdl-31053255

RESUMEN

Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 × g.


Asunto(s)
Gravitación , Espacio Intracelular/metabolismo , Células 3T3 , Animales , Fenómenos Biomecánicos , Compuestos de Boro/metabolismo , Células Endoteliales de la Vena Umbilical Humana/citología , Humanos , Ratones , Viscosidad
3.
Chem Sci ; 10(14): 4069-4076, 2019 Apr 14.
Artículo en Inglés | MEDLINE | ID: mdl-31015948

RESUMEN

Structure-based drug design is commonly used to guide the development of potent and specific enzyme inhibitors. Many enzymes - such as protein kinases - adopt multiple conformations, and conformational interconversion is expected to impact on the design of small molecule inhibitors. We measured the dynamic equilibrium between DFG-in-like active and DFG-out-like inactive conformations of the activation loop of unphosphorylated Aurora-A alone, in the presence of the activator TPX2, and in the presence of kinase inhibitors. The unphosphorylated kinase had a shorter residence time of the activation loop in the active conformation and a shift in the position of equilibrium towards the inactive conformation compared with phosphorylated kinase for all conditions measured. Ligand binding was associated with a change in the position of conformational equilibrium which was specific to each ligand and independent of the kinase phosphorylation state. As a consequence of this, the ability of a ligand to discriminate between active and inactive activation loop conformations was also independent of phosphorylation. Importantly, we discovered that the presence of multiple enzyme conformations can lead to a plateau in the overall ligand K d, despite increasing affinity for the chosen target conformation, and modelled the conformational discrimination necessary for a conformation-promoting ligand.

4.
Biochemistry ; 57(43): 6228-6233, 2018 10 30.
Artículo en Inglés | MEDLINE | ID: mdl-30265526

RESUMEN

The ability of the amyloid-ß peptide to bind to redox active metals and act as a source of radical damage in Alzheimer's disease has been largely accepted as contributing to the disease's pathogenesis. However, a kinetic understanding of the molecular mechanism, which underpins this radical generation, has yet to be reported. Here we use a sensitive fluorescence approach, which reports on the oxidation state of the metal bound to the amyloid-ß peptide and can therefore shed light on the redox kinetics. We confirm that the redox goes via a low populated, reactive intermediate and that the reaction proceeds via the Component I coordination environment rather than Component II. We also show that while the reduction step readily occurs (on the 10 ms time scale) it is the oxidation step that is rate-limiting for redox cycling.


Asunto(s)
Péptidos beta-Amiloides/química , Péptidos beta-Amiloides/metabolismo , Cobre/química , Cobre/metabolismo , Humanos , Concentración de Iones de Hidrógeno , Cinética , Oxidación-Reducción , Estrés Oxidativo
5.
Chembiochem ; 17(18): 1732-7, 2016 09 15.
Artículo en Inglés | MEDLINE | ID: mdl-27356100

RESUMEN

Mutations and post-translational modifications of amyloid-ß (Aß) peptide in its N terminus have been shown to increase fibril formation, yet the molecular mechanism is not clear. Here we investigated the kinetics of the interactions of copper with two Aß peptides containing Familial Alzheimer's disease (FAD) mutations (English (H6R) and Tottori (D7N)), as well as with Aß peptide phosphorylated at serine 8 (pS8). All three peptides bind to copper with a similar rate as the wild-type (wt). The dissociation rates follow the order pS8>H6R>wt>D7N; the interconversion between the two coordinating species occurs 50 % faster for H6R and pS8, whereas D7N had only a negligible effect. Interestingly, the rate of ternary complex (copper-bridged heterodimer) formation for the modified peptides was significantly faster than that for wt, thus leading us to propose that FAD and sporadic AD might share a kinetic origin for the enhanced oligomerisation of Aß.


Asunto(s)
Enfermedad de Alzheimer/genética , Enfermedad de Alzheimer/metabolismo , Péptidos beta-Amiloides/química , Péptidos beta-Amiloides/metabolismo , Cobre/metabolismo , Mutación , Cobre/química , Humanos , Cinética , Fosforilación
6.
Angew Chem Int Ed Engl ; 54(4): 1227-30, 2015 Jan 19.
Artículo en Inglés | MEDLINE | ID: mdl-25529008

RESUMEN

The kinetics of the interactions between amyloid-ß (Aß) and metal ions are crucial to understanding the physiological and pathological roles of Aß in the normal brain and in Alzheimer's disease. Using the quenching of a fluorescent probe by Cu(2+), the mechanism of Aß/Cu(2+) interactions in physiologically relevant conditions has been elucidated. Cu(2+) binds to Aß at a near diffusion-limited rate, initially forming component I. The switching between component I and II occurs on the second timescale, with a significant energy barrier. Component I is much more reactive towards Cu(2+) ligands and likely responsible for initial Aß dimer formation. Clioquinol (CQ) is shown to sequester Cu(2+) more effectively than other tested ligands. These findings have implications for the potential roles of Aß in regulating neurotransmission, and for the screening of small molecules targeting Aß-metal interactions.


Asunto(s)
Péptidos beta-Amiloides/química , Cobre/química , Colorantes Fluorescentes/química , Péptidos beta-Amiloides/metabolismo , Clioquinol/química , Espectroscopía de Resonancia por Spin del Electrón , Iones/química , Cinética
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