[The level of neopterin in blood serum in premature newborn in the process of their adaptation to postnatal life].

The study had a purpose to evaluate the level of serum neopterin in the process of adaptation of healthy newborns to postnatal life. The dynamic clinical monitoring and comparative analysis was applied to determine the level of neopterin in serum of umbilical blood in 46 children and in peripheral blood of their mothers. The immune-enzyme analysis was implemented. It is established that in healthy newborn the content of neopteril reliably exceeds common normative indicator of adults i.e. less than 10 nmol/l. The increased level of neopterin in blood of newborns and their mothers reflects the total reaction of immune response to delivery process. The dependence is established between the level of neopterin and pathologic course of gestation period (acute respiratory viral disease, fetoplacental insufficiency) and morphologic functional maturity of child

[The activity of components of system of matrix metalloproteinase and tissue inhibitors of metalloproteinase on different stages of chronic cardiac failure].

The article discusses the results of detection of activity of matrix metalloproteinase-9, tissue inhibitors of metalloproteinases-4, endotoxin, tumor necrosis factor-alpha, C-reactive protein, sE-selectine in blood of patients with chronic cardiac failure depending on severity of disease. It is demonstrated that progressing of chronic cardiac failure is associated with the increase of level of endotoxemia, activation of systemic inflammation and misbalance in the system of matrix metalloproteinase and tissue inhibitors of metalloproteinase.

[Nonspecific adaptation reactions and immunological status in patients with type 2 diabetes mellitus].

The clinical laboratory study of 75 patients with type 2 diabetes mellitus (T2D) has shown that most of them have elevated immunoglobulin A and G levels, the diminished activity of neutrophiles, and higher C-reactive protein and 30% of the patients show non-physiological adaptation reactions: reactivation and stress. During these reactions, there are the most pronounced changes in the immunological status and in the level of acute phase protein. The rate of nonphysiological reactions increases, immunity deteriorates, and the activity of an inflammatory process is enhanced with the longer duration of T2D, grades 2 and 3 arterial hypertension, micro- and macroangiopathies, as well as with more evident hyperglycemia and triglyceridemia.

[Combined method for testing microflora in oral specimens].

The integrated procedure used in a clinical laboratory to test oral specimens for the microflora, which includes microscopic and rapid cultural studies, enhances the specificity and sensitivity of detection of fungi of the genus Candida as the used chromogenic selective nutrient medium allows C. albicans, C. glabrata, and C. tropocalis to be identified in the primary culture, without increase the time of giving the results of a test. The results of a test for the fungi may be quantitatively presented, which is of importance for the reasonable use of antifungal therapy (only when the diagnostically significant quantity of Candida exceeds) and of value for the control of the results of therapy. Semiquantitative detection of other morphological types (Leptotrichia, Borrelia, Fusobacterium, Protozoon) makes it possible to prescribe etiotropic therapy and to evaluate its efficiency.

[Comparative microanalysis of lipids in various components of the hemostasis system]. / Sravnitel'nyi mikroanaliz lipidov nekotorykh komponentov sistemy gemostaza.

Lipid composition was studied in blood plasma, blood serum and blood clot of healthy persons and of patients with chronic unspecific lung impairments. Similar lipid composition was detected in blood clot (free of cells) and in blood plasma or blood serum where phospholipids and free fatty acids prevailed and constituted about a half of the total lipids content. During formation of blood clot a part of blood plasma lipids was transferred to the clot. Phospholipids and free fatty acids were mainly detected in the blood clot of healthy persons, while in patients with chronic unspecific lungs impairments triglycerides were also detected in the clot. Alterations in lipid composition of blood clot were most informative in patients with chronic unspecific lungs impairments as compared with blood serum and plasma, which might be used for diagnosis of the impairments studied.