RESUMEN
Approximately 400 billion PET bottles are produced annually in the world, of which from 8 to 9 million tons are discarded in oceans. This requires developing strategies to urgently recycle them. PET recycling can be carried out using the microbial hydrolysis of polymers when monomers and oligomers are released. Exploring the metabolic activity of fungi is an environmentally friendly way to treat harmful polymeric waste and obtain the production of monomers. The present study addressed: (i) the investigation of potential of strains with the potential for the depolymerization of PET bottles from different manufacturers (crystallinity of 35.5 and 10.4%); (ii) the search for a culture medium that favors the depolymerization process; and (iii) gaining more knowledge on fungal enzymes that can be applied to PET recycling. Four strains (from 100 fungal strains) were found as promising for conversion into terephthalic acid from PET nanoparticles (npPET): Curvularia trifolii CBMAI 2111, Trichoderma sp. CBMAI 2071, Trichoderma atroviride CBMAI 2073, and Cladosporium cladosporioides CBMAI 2075. The fermentation assays in the presence of PET led to the release of terephthalic acid in concentrations above 12 ppm. Biodegradation was also confirmed using mass variation analyses (reducing mass), scanning electron microscopy (SEM) that showed evidence of material roughness, FTIR analysis that showed band modification, enzymatic activities detected for lipase, and esterase and cutinase, confirmed by monomers/oligomers quantification using high performance liquid chromatography (HPLC-UV). Based on the microbial strains PET depolymerization, the results are promising for the exploration of the selected microbial strain.
RESUMEN
Metal and metalloid concentrations in the liver tissue of green turtles (Chelonia mydas) stranded on the Brazilian coast (n = 506) were studied using inductively coupled plasma mass spectrometry and cold vapor atomic fluorescence spectrometry. The influences of occurrence registers (date and location) and biological characteristics (sex, age, and developmental stage) were assessed, as well as the temporal influences of oil exploration and production activities. The mean concentrations of Cd, Cu, Mn, Zn, and Hg were the highest reported for the liver of C. mydas on the Brazilian coast. The mean element concentrations followed the order: Cu > Zn > Cd > Mn > As > Hg > Mo > Pb > V > Ni > Ba > Cr. Further, significant differences (p < 0.05) were observed for Hg between the sexes (males > females) and for As, Cu, Pb, Mo, and V between young individuals and older individuals (≥11 years), suggesting a relationship between the dietary shift inherent to green turtle development. These results were corroborated by the curved carapace length (CCL) data, wherein individuals residing in coastal areas (CCL > 50 cm) presented higher concentrations of Cu, Pb, Mo, Zn, Ba, and V than those in the oceanic stage (CCL < 30 cm). The opposite pattern was observed for As and Hg. The influences of spatial autocorrelation (Moran Index) at a global scale and oil production activities on the element concentrations were not observed. However, five hotspots of high metal concentrations were identified via a local spatial autocorrelation (local indicator of spatial association), existing predominantly in a region of heavy anthropic activity within the sampling area. Further, baseline element concentrations were established at the 95% confidence level. Overall, the developmental stage, which is related to feeding habits, had an expressive influence on element concentrations.
Asunto(s)
Metaloides , Metales Pesados , Tortugas , Contaminantes Químicos del Agua , Animales , Brasil , Monitoreo del Ambiente , Femenino , Hígado/química , Masculino , Metales Pesados/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
Os exergames (EXGs), jogos virtuais que englobam o movimento humano em sua prática, têm funcionado atualmente como um intermédio entre a vida extremamente tecnológica e a inatividade física. Os objetivos do presente estudo foram identificar os principais motivos pelos quais as pessoas jogam videogames e EXGs, correlacionando-os aos motivos pelos quais elas praticam atividades físicas e esportes. Participaram do estudo 18 voluntários de ambos os gêneros, com idade média de 22,7 ± 2,4 anos. Foi utilizado o Inventário de Motivação, Jogos Virtuais e Prática de Atividades Físicas/Esportes. Os resultados proporcionaram estabelecer conexões entre a influência das novas tecnologias, com enfoque específico nos jogos virtuais e EXGs, os estados emocionais, mais especificamente a motivação e a prática de exercícios e atividades físicas.
The exergames (EXGs), virtual games that involve the human movement in their practice, have now acted as an intermediary between highly technological life and physical inactivity. The objectives of this study were to identify the main reasons why people play video games and EXGs, correlating them to why they practice physical activities and sports. Study participants were 18 volunteers of both genders, with a mean age of 22.7 ± 2.4 years. Inventory of Motivation, Virtual Games and Practice of Physical Activities/Sports was used. The results provide connections between the influence of new technologies, with a specific focus on virtual and EXGs games, emotional states, specifically the motivation and the practice of physical exercises and activities.
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Actividades RecreativasRESUMEN
Based on Global Network Isotopes in Precipitation (GNIP) isotopic data set, a review of the spatial and temporal variability of δ18O and δ2H in precipitation was conducted throughout central and eastern Brazil, indicating that dynamic interactions between Intertropical and South Atlantic Convergence Zones, Amazon rainforest, and Atlantic Ocean determine the variations on the isotopic composition of precipitation over this area. Despite the seasonality and latitude effects observed, a fair correlation with precipitation amount was found. In addition, Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) air mass back trajectories were used to quantify the factors controlling daily variability in stable isotopes in precipitation. Through a linear multiple regression analysis, it was observed that temporal variations were consistent with the meteorological parameters derived from HYSPLIT, particularly precipitation amount along the trajectory and mix depth, but are not dependent on vapour residence time in the atmosphere. These findings also indicate the importance of convective systems to control the isotopic composition of precipitation in tropical and subtropical regions.
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Monitoreo del Ambiente , Lluvia , Movimientos del Aire , Brasil , Deuterio , Isótopos de Oxígeno , Estaciones del AñoRESUMEN
This manuscript reports an evaluation of the effects of simple chemical-heat treatments on the deposition of different ceramic coatings, i.e., TiO2, CaTiO3 and CaP, on commercially pure titanium (cp-Ti) and Ti6Al4V and the influence of the coatings on cells interaction with the surfaces. The ceramic materials were prepared by the sol-gel method and the coating adhesion was analyzed by pull-off bending tests. The wettability of positively or negatively charged surfaces was characterized by contact angle measurements, which also enabled the calculation of the surface free energy through the polar-apolar liquids approach. Both acid and alkaline treatments activated the cp-Ti, whereas Ti6Al4V was only activated by the alkaline treatment. Such treatment led to increased hydrophilicity with inhibition of the fibroblastic response on Ti6Al4V. On the other hand, osteoblastic cells adhered to and proliferated on the positively and negatively charged surfaces. The maximum adhesion strength (~ 3400 N) was obtained with a negative Ti6Al4V-CaTiO3-CaP multilayer surface.
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Materiales Biocompatibles Revestidos/química , Fibroblastos/efectos de los fármacos , Osteoblastos/efectos de los fármacos , Titanio/química , Aleaciones , Animales , Biomarcadores/química , Compuestos de Calcio/química , Adhesión Celular , Proliferación Celular , Cerámica/química , Durapatita/química , Ratones , Osteoblastos/citología , Óxidos/química , Polímeros/química , Conejos , Estrés Mecánico , Propiedades de Superficie , Adherencias Tisulares , HumectabilidadRESUMEN
BACKGROUND: Data on the relationship between behavioral disturbances in Alzheimer's disease (AD) and global clinical deterioration is still controversial. The purpose of this study was to explore potential correlations of neuropsychiatric syndromes with global clinical deterioration in patients with AD, with particular consideration on severity levels of dementia. METHODS: AD patients (n = 156) aged 76.7 years from Brazilian clinical centers were assessed to diagnose the five neuropsychiatric syndromes measured by the Neuropsychiatric Inventory-Clinician rating scale (NPI-C): psychosis, agitation, affective, apathy, and sleep. These syndromes were then analyzed for their correlation with the Global Deterioration Scale (GDS). To analyze the association of neuropsychiatric syndromes with the GDS, considering the total sample and patients grouped by dementia severity levels, we applied the coefficient of multiple correlation (Ryy), adjusted multiple linear regression, and the coefficient of determination (R2yx). We tested the significance of correlation coefficients using the Student t-test for simple correlations (a single independent variable) and analysis of variance (ANOVA) for multiple correlations. ANOVA was also used to compare means of demographic and some clinical variables at different levels of dementia. RESULTS: For the total sample, apathy and agitation syndromes were most strongly correlated (0.74; 0.72, respectively) with clinical deterioration according to the GDS, followed by psychosis (0.59), affective (0.45), and sleep syndromes (0.34). Agitation significantly correlated with mild and moderate dementia (CDR 1: 0.45; and CDR 2: 0.69, respectively). At CDR 2, agitation and affective syndromes were most strongly correlated (0.69; 0.59, respectively) with clinical deterioration while at CDR 3, the apathy syndrome was most strongly correlated with clinical deterioration (0.52). CONCLUSIONS: Agitation, apathy, and affective disorders were the syndromes most strongly correlated with global deterioration in AD patients, becoming more evident at severe stages of dementia.
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Enfermedad de Alzheimer/psicología , Apatía , Agitación Psicomotora/epidemiología , Anciano , Anciano de 80 o más Años , Brasil , Femenino , Humanos , Modelos Lineales , Masculino , Pruebas Neuropsicológicas , Inventario de Personalidad , Escalas de Valoración PsiquiátricaRESUMEN
BACKGROUND: The measurement of neuropsychiatric symptoms (NPS) in dementia is often based on caregiver report. Challenges associated with providing care may bias the caregiver's recognition and reporting of symptoms. Given potential problems associated with caregiver report, clinicians may improve measurement by drawing from a wider array of available data and by applying clinical judgment. OBJECTIVE: The objective of this study is to evaluate potential disagreements between caregiver report and clinician impression when rating psychopathological manifestations from the same patient with dementia. METHODS: Three hundred twelve participants (156 patients with Alzheimer's disease [AD] and 156 caregivers) were studied using the Neuropsychiatric Inventory-Clinician Rating Scale. We considered disagreement to be present when caregiver ratings were significantly higher or lower (p < 0.05) than NPS ratings by clinicians of the same patient. To evaluate whether disagreements were related to dementia severity, we repeated comparisons across levels defined by the clinical dementia rating. RESULTS: The most common disagreements involved ratings of agitation, depression, anxiety, apathy, irritability, and aberrant motor behavior especially in patients with mild dementia. There were fewer discrepancies in moderate or severe dementia. The most consistent disagreements involved global ratings of depression where caregiver scores ranged from +22.5 higher to -4.5 lower than clinician rating. CONCLUSIONS: Caregivers may have incomplete perception of patient NPS mainly in mild dementia. NPS ratings might be confounded by cultural beliefs, sometimes leading caregiver to interpret symptoms as part of "normal" aging.
Asunto(s)
Enfermedad de Alzheimer/diagnóstico , Cuidadores , Pruebas Neuropsicológicas , Anciano , Anciano de 80 o más Años , Enfermedad de Alzheimer/psicología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Pruebas Neuropsicológicas/normas , Variaciones Dependientes del Observador , Escalas de Valoración Psiquiátrica , Calidad de Vida , Índice de Severidad de la EnfermedadRESUMEN
BACKGROUND: The potential attraction from 1-octen-3-ol for sandflies has been documented; however, studies using other primary alcohols are limited. FINDINGS: We used a wind tunnel to compare the activation and attractive behaviors in male and female Lutzomyia longipalpis using 1-octen-3-ol and three additional alcohols, 1-octanol, 1-heptanol and 1-nonanol at three different concentrations: neat (100%) and diluted in hexane (10% and 50%). The compounds 1-octen-3-ol and 1-nonanol induced a clear concentration-dependent activation and attraction response in females. In males, 1-octen-3-ol, 1-nonanol and 1-heptanol yielded the same results. CONCLUSIONS: L. longipalpis is attracted to 1-octen-3-ol, 1-nonanol and 1-heptanol, which are found in many plant volatiles.
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Alcoholes , Psychodidae/fisiología , Animales , Femenino , Control de Insectos , Masculino , Compuestos Orgánicos VolátilesRESUMEN
This study aimed to evaluate the mental conditions of cocaine-dependent individuals and school commitment/attachment. We evaluated 50 patients referred to the psychiatry emergency room due to mental disorders from chemical dependence. After clinical diagnosis, clinical interview, Hospital Anxiety and Depression Scale, Hamilton Scale for Depression and Brief Psychiatric Rating Scale were applied. The Spearman and Mann-Whitney nonparametric tests, as well as the t-Student test were utilized for statistical analysis.. The accepted significance value was 0.05. All subjects had used cocaine or crack and other substances. Only 13 (26 percent) did not drop out of school (group 1). Regarding the other 37 (74 percent), irregular class attendance , successive failures and definitive school drop out rates (group 2) were verified. These subjects presented an early substance use when compared with those which did not drop out of school (p=0.0001). Patients with an early substance use presented higher school dropout rates than those with a later initiation to substance use. Psychopathological phenomena were frequent in both groups.
El objetivo del estudio fue evaluar la relación entre las condiciones mentales de dependientes de sustancias psicoativas y el vínculo escolar. Fueron evaluados 50 participantes atendidos en emergencias psiquiátricas por trastornos mentales asociados a la dependencia química. Luego del diagnóstico clínico, fueron realizadas las siguientes estrategias metodológicas: Entrevista Clínica, Escala Hospitalaria de Ansiedad y Depresión, Escala de Depresión de Hamilton y Escala Psiquiátrica Breve. La análisis estadístico se expresó mediante testes no-paramétricos como correlación de Spearman, Mann-Whitney, y t-Student test, con nivel de significación de 0,05. Todos los participantes hacían uso de cocaína o crack con otras sustancias. Apenas 13 (26 por ciento) no interrumpen el vínculo escolar (grupo 1). Los demás, 37 participantes (74 por ciento), verificaron frecuencia irregular en aula, sucesivas reprobaciones y interrupción definitiva del vínculo escolar (grupo 2). Estos últimos participantes (con interrupción del vínculo escolar) habían iniciado el uso de sustancias psicoativas más precozmente cuando los comparamos con los primeros (sin interrupción del vínculo escolar) con diferencia significativa entre ambos (p=0,0001). En conclusión, los participantes que iniciaron tempranamente el uso de sustancias psicoativas presentaban tasas mayores de interrupción del vínculo escolar quando comparados con aquejes con inicio más tardío. Los fenómenos psicopatológicos eran frecuentes y graves en ambos grupos.
RESUMEN
El objetivo del estudio fue evaluar la relación entre las condiciones mentales de dependientes de sustancias psicoativas y el vínculo escolar. Fueron evaluados 50 participantes atendidos en emergencias psiquiátricas por trastornos mentales asociados a la dependencia química. Luego del diagnóstico clínico, fueron realizadas las siguientes estrategias metodológicas: Entrevista Clínica, Escala Hospitalaria de Ansiedad y Depresión, Escala de Depresión de Hamilton y Escala Psiquiátrica Breve. La análisis estadístico se expresó mediante testes no-paramétricos como correlación de Spearman, Mann-Whitney, y t-Student test, con nivel de significación de 0,05. Todos los participantes hacían uso de cocaína o crack con otras sustancias. Apenas 13 (26
) no interrumpen el vínculo escolar (grupo 1). Los demás, 37 participantes (74
), verificaron frecuencia irregular en aula, sucesivas reprobaciones y interrupción definitiva del vínculo escolar (grupo 2). Estos últimos participantes (con interrupción del vínculo escolar) habían iniciado el uso de sustancias psicoativas más precozmente cuando los comparamos con los primeros (sin interrupción del vínculo escolar) con diferencia significativa entre ambos (p=0,0001). En conclusión, los participantes que iniciaron tempranamente el uso de sustancias psicoativas presentaban tasas mayores de interrupción del vínculo escolar quando comparados con aquejes con inicio más tardío. Los fenómenos psicopatológicos eran frecuentes y graves en ambos grupos (AU)
Asunto(s)
Trastornos Relacionados con Sustancias , Competencia Mental , EducaciónRESUMEN
The automated Bioscreen C system was used for growth of four Mucor hiemalis strains isolated from the soil in the Juréia-Itatins Ecology Station (JIES), São Paulo state, in liquid culture media containing different carbon (2 percent) and nitrogen (1 percent) sources, pH 5.0, at 25°C, and agitated at 150 rpm for 5 days. The medium containing only one nitrogen source had been added with 2 percent glucose. The readings were taken at lambda = 540 nm, at 2-hour intervals, up to five days. The results were compared using the Friedman Test (alpha = 5 percent). The best growth was obtained for strains M1, M2 and M3, reaching the log phase in 60 hours. The best carbon sources varied according to the strain, and yeast extract proved to be the best nitrogen source. Sodium acetate inhibited the growth of the four strains, being the M3 strain the most affected. The use of the automated system was very convenient for cultures in liquid media, as it is rapid and automated, providing a good technique for determination of the optimal environmental factors for growth of the filamentous fungi.
O sistema automatizado Bioscreen C foi utilizado para o crescimento de quatro linhagens de Mucor hiemalis, isoladas do solo da Estação Ecológica de Juréia-Itatins (EEJI), estado de São Paulo, em meios líquidos com uma única fonte de carbono (2 por cento) ou de nitrogênio (1 por cento), pH 5,0, a 25°C, e agitação de 150rpm por 5 dias. O meio com somente uma única fonte de nitrogênio foi adicionado com 2 por cento de glicose. As leituras de densidade óptica foram realizadas a 540nm, em intervalos de 2h, por cinco dias. Os resultados foram analisados estatisticamente com o Teste de Friedman (alfa = 5 por cento). Os melhores crescimentos foram obtidos com as linhagens M1, M2 e M3, que atingiram o início da fase log em 60 horas de cultivo. As melhores fontes de carbono variaram de acordo com a linhagem estudada, e extrato de levedura provou ser a melhor fonte de nitrogênio para todas as linhagens. Acetato de sódio inibiu o crescimento das quatro linhagens, sendo a M3 a mais afetada. O uso do sistema automatizado foi muito conveniente para as culturas em meio liquido, sendo rápido e automático, constituindo em uma boa técnica para a determinação das condições ambientais ótimas para crescimento de fungos filamentosos.