RESUMEN
The current study describes a novel species with the strain name ERMR1:05T isolated from the forefield soil of East Rathong Glacier in West Sikkim Himalaya (India). The isolate was facultatively anaerobic, gram-stain negative, non-spore-forming, rod-shaped, and oxidase negative. Whole-genome-based bacterial core gene phylogenetic analysis placed the strain in the genus Rahnella, well separated from Rouxiella spp. The digital DNA-DNA hybridisation and average nucleotide identity values between strain ERMR1:05T and other members of genus Rahnella were below the proposed thresholds for the species delineation. Based on these results, a new species, Rahnella sikkimica sp. nov., is proposed with strain ERMR1:05T (CIP 111636T, MTCC 12598T) as the type strain. The bacterium showed upregulation of cold-stress genes in cold conditions. Additionally, the genome analysis of the bacterium showed the presence of plant growth-promotion factors suggesting its role in crop improvement in cold hilly regions.
Asunto(s)
Cubierta de Hielo , Rahnella , Cubierta de Hielo/microbiología , Rahnella/genética , ARN Ribosómico 16S/genética , Filogenia , ADN Bacteriano/genética , Técnicas de Tipificación Bacteriana , Composición de Base , Sikkim , Análisis de Secuencia de ADN , Ácidos Grasos , FosfolípidosRESUMEN
The genus Serratia has been studied for over a century and includes clinically-important and diverse environmental members. Despite this, there is a paucity of genomic information across the genus and a robust whole genome-based phylogenetic framework is lacking. Here, we have assembled and analysed a representative set of 664 genomes from across the genus, including 215 historic isolates originally used in defining the genus. Phylogenomic analysis of the genus reveals a clearly-defined population structure which displays deep divisions and aligns with ecological niche, as well as striking congruence between historical biochemical phenotyping data and contemporary genomics data. We highlight the genomic, phenotypic and plasmid diversity of Serratia, and provide evidence of different patterns of gene flow across the genus. Our work provides a framework for understanding the emergence of clinical and other lineages of Serratia.
Asunto(s)
Genoma Bacteriano , Genómica , Genoma Bacteriano/genética , Filogenia , Plásmidos , Serratia/genéticaRESUMEN
The seventh cholera pandemic has heavily affected Africa, although the origin and continental spread of the disease remain undefined. We used genomic data from 1070 Vibrio cholerae O1 isolates, across 45 African countries and over a 49-year period, to show that past epidemics were attributable to a single expanded lineage. This lineage was introduced at least 11 times since 1970, into two main regions, West Africa and East/Southern Africa, causing epidemics that lasted up to 28 years. The last five introductions into Africa, all from Asia, involved multidrug-resistant sublineages that replaced antibiotic-susceptible sublineages after 2000. This phylogenetic framework describes the periodicity of lineage introduction and the stable routes of cholera spread, which should inform the rational design of control measures for cholera in Africa.
Asunto(s)
Cólera/epidemiología , Cólera/microbiología , Pandemias , Vibrio cholerae O1/clasificación , Vibrio cholerae O1/genética , África Oriental/epidemiología , África Austral/epidemiología , África Occidental/epidemiología , Asia/epidemiología , Genoma Bacteriano , Genómica , Humanos , Filogenia , Vibrio cholerae O1/aislamiento & purificaciónRESUMEN
Latin America has experienced two of the largest cholera epidemics in modern history; one in 1991 and the other in 2010. However, confusion still surrounds the relationships between globally circulating pandemic Vibrio cholerae clones and local bacterial populations. We used whole-genome sequencing to characterize cholera across the Americas over a 40-year time span. We found that both epidemics were the result of intercontinental introductions of seventh pandemic El Tor V. cholerae and that at least seven lineages local to the Americas are associated with disease that differs epidemiologically from epidemic cholera. Our results consolidate historical accounts of pandemic cholera with data to show the importance of local lineages, presenting an integrated view of cholera that is important to the design of future disease control strategies.
Asunto(s)
Cólera/epidemiología , Cólera/microbiología , Pandemias/prevención & control , Vibrio cholerae/clasificación , Vibrio cholerae/genética , Cólera/prevención & control , Control de Enfermedades Transmisibles , Farmacorresistencia Bacteriana Múltiple , Humanos , América Latina/epidemiología , Análisis de Secuencia de ADN , Vibrio cholerae/aislamiento & purificaciónRESUMEN
Four bacterial strains isolated from peat bog soil or swampy meadow in Baden-Württemberg (Germany) and found to have rrs sequences close to that of Rouxiella chamberiensis were compared to this species by using multi-locus sequence analysis and phenotypic tests. The four strains constituted two discrete groups (referred to as the Baden and the Silva groups) belonging to the genus Rouxiella. These groups differed in their ability to grow at 37 °C, reduce nitrate into nitrite, and to produce acid from several carbohydrates. Two novel species are, therefore, proposed: Rouxiella badensis sp. nov. for the Baden group (type strain, 323T=CIP 111153T=DSM 100043T) and Rouxiella silvae for the Silva group (type strain, 213T=CIP 111154T=DSM 103735T). The definition of the genus Rouxiellahas also been emended in order to take these two novel species into account.
Asunto(s)
Gammaproteobacteria/clasificación , Filogenia , Microbiología del Suelo , Humedales , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Gammaproteobacteria/genética , Gammaproteobacteria/aislamiento & purificación , Alemania , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
Six isolates recovered from coffee seeds giving off a potato-like flavour were studied. Gene sequencing (rrs and rpoB) showed they belong to the genus Pantoea. By DNA-DNA hybridization, the isolates constituted a genomic species with less than 17% relatedness to 96 strains representing enterobacterial species. Multilocus sequence analysis (gyrB, rpoB, atpD and infB genes) showed the isolates to represent a discrete species of the genus Pantoea. Nutritional versatility of the novel species was poor. The novel species is proposed as Pantoea coffeiphila sp.nov. and its type strain is Ca04(T) (â=CIP 110718(T)â=DSM 28482(T)).
Asunto(s)
Café/microbiología , Microbiología de Alimentos , Pantoea/clasificación , Filogenia , Gusto , Técnicas de Tipificación Bacteriana , Burundi , ADN Bacteriano/genética , Contaminación de Alimentos , Genes Bacterianos , Datos de Secuencia Molecular , Tipificación de Secuencias Multilocus , Hibridación de Ácido Nucleico , Pantoea/genética , Pantoea/aislamiento & purificación , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
Strains previously classified as members of Klebsiella pneumoniae phylogroups KpI, KpII-A, KpII-B and KpIII were characterized by 16S rRNA (rrs) gene sequencing, multilocus sequence analysis based on rpoB, fusA, gapA, gyrA and leuS genes, average nucleotide identity and biochemical characteristics. Phylogenetic analysis demonstrated that KpI and KpIII corresponded to K. pneumoniae and Klebsiella variicola, respectively, whereas KpII-A and KpII-B formed two well-demarcated sequence clusters distinct from other members of the genus Klebsiella. Average nucleotide identity between KpII-A and KpII-B was 96.4â%, whereas values lower than 94â% were obtained for both groups when compared with K. pneumoniae and K. variicola. Biochemical properties differentiated KpII-A, KpII-B, K. pneumoniae and K. variicola, with acid production from adonitol and l-sorbose and ability to use 3-phenylproprionate, 5-keto-d-gluconate and tricarballylic acid as sole carbon sources being particularly useful. Based on their genetic and phenotypic characteristics, we propose the names Klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and K. quasipneumoniae subsp. similipneumoniae subsp. nov. for strains of KpII-A and KpII-B, respectively. The type strain of K. quasipneumoniae sp. nov. and of K. quasipneumoniae subsp. quasipneumoniae subsp. nov. is 01A030(T) (â=âSB11(T)â=âCIP 110771(T)â=âDSM 28211(T)). The type strain of K. quasipneumoniae subsp. similipneumoniae subsp. nov. is 07A044(T) (â=âSB30(T)â=âCIP 110770(T)â=âDSM 28212(T)). Both strains were isolated from human blood cultures. This work also showed that Klebsiella singaporensis is a junior heterotypic synonym of K. variicola.
Asunto(s)
Infecciones por Klebsiella/microbiología , Klebsiella/clasificación , Filogenia , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Genes Bacterianos , Humanos , Klebsiella/genética , Klebsiella/aislamiento & purificación , Datos de Secuencia Molecular , Tipificación de Secuencias Multilocus , ARN Ribosómico 16S/genéticaRESUMEN
An environmental freshwater bacterial isolate, DM104, appearing as Shigella-like colonies on selective agar plates was found to show strong and specific serological cross-reactivity with Shigella dysenteriae type 4. Biochemical identification according to the analytical profile index, molecular serotyping by restriction of the amplified O-antigen gene cluster (rfb-RFLP), together with phylogenetic analysis of the 16S rRNA gene and multi-locus sequence analysis, identified the isolate as Escherichia albertii. rfb-RFLP of DM104, revealed a profile different from that of S. dysenteriae type 4. However, western blot analysis of extracted lipopolysaccharides demonstrated strong cross-reactivity with S. dysenteriae type 4 using specific monovalent antisera and a lipopolysaccharide gel banding profile similar to that of S. dysenteriae type 4. The observed O-antigen cross-reaction between an E. albertii isolate and S. dysenteriae extends our knowledge of the extent of O-antigen cross-reaction within the Escherichia/Shigella group of organisms, and offers the possibility of using DM104 and similar cross-reacting strains as shigellosis vaccine candidates.
Asunto(s)
Reacciones Cruzadas/inmunología , Escherichia/inmunología , Antígenos O/inmunología , Shigella dysenteriae/inmunología , Escherichia/clasificación , Escherichia/genética , Genes Bacterianos , Genes Esenciales , Antígenos O/genética , Filogenia , Serotipificación , Shigella dysenteriae/clasificación , Shigella dysenteriae/genéticaRESUMEN
Phylogenetic analysis of partial rpoB gene sequences of type and clinical strains belonging to different 16S rRNA gene-fingerprinting ribogroups within 11 species of enterobacteria of the genera Proteus, Morganella and Providencia was performed and allowed the definition of rpoB clades, supported by high bootstrap values and confirmed by ≥2.5â% nucleotide divergence. None of the resulting clades included strains belonging to different species and the majority of the species were confirmed as discrete and homogeneous. However, more than one distinct rpoB clade could be defined among strains belonging to the species Proteus vulgaris (two clades), Providencia alcalifaciens (two clades) and Providencia rettgeri (three clades), suggesting that some strains represent novel species according to the genotypes outlined by rpoB gene sequence analysis. Percentage differences between the rpoB gene sequence of the type strain of Proteus myxofaciens and other members of the same genus (17.3-18.9â%) were similar to those calculated amongst strains of the genus Providencia (16.4-18.7â%), suggesting a genetic distance at the genus-level between Proteus myxofaciens and the rest of the Proteus-Providencia group. Proteus myxofaciens therefore represents a member of a new genus, for which the name Cosenzaea gen. nov., is proposed.
Asunto(s)
ARN Polimerasas Dirigidas por ADN/genética , Morganella/clasificación , Filogenia , Proteus/clasificación , Providencia/clasificación , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Genes Bacterianos , Datos de Secuencia Molecular , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
A Listeria-like strain isolated in Austria from pre-cut lettuce fitted the description of the genus Listeria although it could not be assigned to any of the known species. Comparison of the rrs gene (encoding 16S rRNA) sequence and gene content by DNA-array indicated affiliation to the genus Listeria. Phylogenetic distance from known species of the genus Listeria indicated that it represents a novel species. Since it can be differentiated from all other known species of the genus Listeria by using phenotypic tests, the name Listeria rocourtiae sp. nov. is proposed for the novel species. The type strain is CIP 109804(T) (=DSM 22097(T) =Allerberger 700284/02(T)). The type strain is avirulent as assessed by cell culture assays and inoculation of mice.
Asunto(s)
Lactuca/microbiología , Listeria/clasificación , Listeria/aislamiento & purificación , ADN Bacteriano/genética , ADN Ribosómico/genética , Listeria/genética , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genéticaRESUMEN
This supplement reports the characterization of 70 new Salmonella serovars recognized between 2003 and 2007 by the WHO Collaborating Center for Reference and Research on Salmonella: 44 were assigned to Salmonella enterica subspecies enterica, 11 to subspecies salamae, 5 to subspecies arizonae, 8 to subspecies diarizonae, one to subspecies houtenae and one to Salmonella bongori. One new serovar, Mygdal, displayed a new H factor, H:z(91).
Asunto(s)
Técnicas Bacteriológicas/métodos , Salmonelosis Animal/microbiología , Infecciones por Salmonella/microbiología , Salmonella enterica/clasificación , Salmonella enterica/aislamiento & purificación , Animales , Humanos , SerotipificaciónRESUMEN
In 1993, after 6 years of absence, cholera re-emerged in the Horn of Africa. Following its introduction to Djibouti, the disease spread to the central and southern areas of Ethiopia reaching Somalia in 1994. Cholera outbreaks persisted in Ethiopia with a recrudescence of cases in 1998. Twenty-two Vibrio cholerae O1 strains, selected to represent the 1998 history of cholera in Ethiopia, were characterized by random amplified polymorphic DNA patterns, BglI ribotyping and antimicrobial susceptibility. All isolates showed a unique amplified DNA pattern and a prevalent ribotype B8a. All strains were multidrug-resistant and harboured an IncC plasmid which conferred resistance to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole and trimethoprim. These findings indicate that a group of closely related V. cholerae O1 strains was responsible for the cholera epidemic in Ethiopia in 1998.
Asunto(s)
Cólera/epidemiología , Cólera/historia , Brotes de Enfermedades , Vibrio cholerae O1/aislamiento & purificación , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Antibacterianos/farmacología , Niño , Preescolar , Dermatoglifia del ADN , Farmacorresistencia Bacteriana Múltiple , Etiopía/epidemiología , Femenino , Historia del Siglo XX , Humanos , Lactante , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Técnica del ADN Polimorfo Amplificado Aleatorio , Ribotipificación , Vibrio cholerae O1/clasificación , Vibrio cholerae O1/efectos de los fármacos , Vibrio cholerae O1/genética , Adulto JovenRESUMEN
One hundred and three Vibrio cholerae O1 strains, selected to represent the cholera outbreaks which occurred in Somalia in 1998-1999, were characterized by random amplified polymorphic DNA patterns, ribotyping, and antimicrobial susceptibility. All strains showed a unique amplified DNA pattern and 2 closely related ribotypes (B5a and B8a), among which B5a was the more frequently identified. Ninety-one strains were resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfamethoxazole, and trimethoprim, conferred, except for spectinomycin, by a conjugative plasmid IncC. These findings indicated that the group of strains active in Somalia in the late 1990s had a clonal origin.
Asunto(s)
Cólera/epidemiología , Cólera/microbiología , Vibrio cholerae O1/clasificación , Vibrio cholerae O1/aislamiento & purificación , Antibacterianos/farmacología , Técnicas de Tipificación Bacteriana , Análisis por Conglomerados , Conjugación Genética , Dermatoglifia del ADN , ADN Bacteriano/química , ADN Bacteriano/genética , Brotes de Enfermedades , Farmacorresistencia Bacteriana , Genotipo , Humanos , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Plásmidos , Técnica del ADN Polimorfo Amplificado Aleatorio , Ribotipificación , Análisis de Secuencia de ADN , Somalia/epidemiología , Vibrio cholerae O1/genéticaRESUMEN
The multidrug-resistant (MDR) Salmonella enterica serotype Newport strain that produces CMY-2 beta-lactamase (Newport MDR-AmpC) was the source of sporadic cases and outbreaks in humans in France during 2000-2005. Because this strain was not detected in food animals, it was most likely introduced into France through imported food products.
Asunto(s)
Antibacterianos/farmacología , Ceftriaxona/farmacología , Farmacorresistencia Bacteriana , Infecciones por Salmonella/epidemiología , Salmonella enterica/efectos de los fármacos , beta-Lactamasas/biosíntesis , Animales , Comercio , Farmacorresistencia Bacteriana/genética , Electroforesis en Gel de Campo Pulsado , Francia/epidemiología , Humanos , Carne/microbiología , Infecciones por Salmonella/microbiología , Salmonella enterica/clasificación , Salmonella enterica/enzimología , Salmonella enterica/genética , Análisis de Secuencia de ADN , Serotipificación , beta-Lactamasas/genéticaRESUMEN
Phylogenetic analysis of strains from Burkholderia, Ralstonia, Cupriavidus, Comamonas, Delftia, Acidovorax, Brevundimonas, Herbaspirillum huttiense and "Pseudomonas butanovora" was performed based on the protein-coding genes rpoB and gyrB and on the 16S rRNA-coding gene rrs. Overall, the phylogenies deduced from the three genes were concordant among themselves and with current taxonomy. However, a few differences among individual gene phylogenies were noted. For example, the separation of Cupriavidus from Ralstonia was not supported in the rpoB tree, as Ralstonia was nested within Cupriavidus. Similarly, the separation of Delftia from Comamonas was not supported in the gyrB tree. Based on rrs and rpoB, the genus Burkholderia contained four groups: (i) the B. cepacia complex, (ii) the B. pseudomallei-B. thailandensis group, (iii) a 6-species group including B. caledonica and B. glathei and (iv) the B. plantarii-B. glumae-B. gladioli group. However, B. caribensis and B. glathei stood as a fifth group based on gyrB. It appears that a phylogeny cannot be reliably based on a single gene. Using rpoB and gyrB, better separation of closely related species was obtained compared to rrs, indicating the potential of these two genes for identification and species definition. Nevertheless, intraspecific sequence diversity will need to be determined to fully establish the value of these genes for strain identification.
Asunto(s)
Bacterias/clasificación , Proteínas Bacterianas/genética , Girasa de ADN/genética , Filogenia , ARN Ribosómico 16S/genética , Bacterias/genética , Técnicas de Tipificación Bacteriana , Biodiversidad , ADN Bacteriano/genética , ADN Ribosómico/genética , Datos de Secuencia Molecular , Análisis de Secuencia de ADNRESUMEN
Salmonella enterica serotype Typhi clinical isolates (n = 91) resistant to nalidixic acid (Nal(r)) were collected from sporadic cases and minor outbreaks throughout Vietnam between 1996 and 2004. These isolates were typed and compared by four methods: Vi phage typing, PstI ribotyping, XbaI and SpeI pulsed-field gel electrophoresis (PFGE), and single-nucleotide polymorphism (SNP) analysis. The results indicated that 65% of the isolates were not typeable by Vi phage typing. In contrast, the ribotyping and, with more accuracy, the SNP analysis methods indicated that all Nal(r) isolates belonged to a single clone (ribotype 3a, haplotype H58) that was found previously and that largely consisted of plasmid-encoded multidrug-resistant serotype Typhi isolates. PFGE demonstrated the occurrence of microevolution within this clone. We identified two major combined PFGE profiles: X1-S1 and X3-S6. X3-S6 predominated between 1996 and 2002 but was replaced by X1-S1 after 2002. Nevertheless, PFGE, with a Simpson's index of 0.78, was not considered an optimal discriminatory method for investigating typhoid fever outbreaks in Vietnam. The rate of quinolone resistance increased and the rate of multidrug resistance decreased during the study period. From 2002 to 2004, 80.6% of the isolates from South Vietnam were resistant only to Nal. The mechanism of Nal resistance in most of the isolates (94%) was a mutation in the quinolone resistance-determining chromosomal region of gyrA that led to the amino acid substitution Ser83Phe. No plasmid-located qnrA, qnrB, or qnrS was detected.
Asunto(s)
Quinolonas/farmacología , Salmonella typhi/clasificación , Tipificación de Bacteriófagos , Farmacorresistencia Bacteriana Múltiple , Electroforesis en Gel de Campo Pulsado , Evolución Molecular , Pruebas de Sensibilidad Microbiana , Polimorfismo de Nucleótido Simple , Ribotipificación , Salmonella typhi/efectos de los fármacos , Salmonella typhi/genética , Factores de Tiempo , VietnamRESUMEN
We studied the prevalence of resistance to extended-spectrum cephalosporins (ESC) among 1,078 Salmonella enterica isolates collected from adults admitted to Botkin Hospital, St. Petersburg, Russia, for gastroenteritis between 2002 and 2005. Only two ESC-resistant isolates were detected, giving a low percentage of strains resistant to ESC (0.2%). One multidrug-resistant (MDR) isolate of the Virchow serotype produced a CTXM-3 extended-spectrum beta-lactamase (ESBL). The bla(CTX-M-3) gene was located downstream from an ISEcp1 element, on an 80-kb conjugative plasmid. The Virchow isolate possessed a class 1 integron with a 2.2-kb gene cassette (dhfrXII-orfF-aadA2). The second ESC-resistant isolate belonged to serotype Newport, was also MDR and produced a CMY-2 cephamycinase. This CMY-2-producing isolate (also called Newport MDR-AmpC) possessed a class 1 integron with a 1-kb gene cassette including a new variant of the aadA gene, aadA24. A large plasmid (>125 kb) was involved in transfer of the bla(CMY-2) gene. The ESC-resistant S. enterica isolates detected in this study were different from those (S. enterica serotype Typhimurium DT193 producing CTXM-4 or CTX-M-5 ESBLs) involved in several nosocomial outbreaks between 1994 and 2003 in Russia. This is the first description of both CTX-M-3 ESBL-producing S. enterica and Newport MDR-AmpC in Russia.
Asunto(s)
Antibacterianos/farmacología , Cefalosporinas/farmacología , Farmacorresistencia Bacteriana , Salmonella enterica/efectos de los fármacos , Adulto , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/genética , Infección Hospitalaria/epidemiología , Infección Hospitalaria/microbiología , Brotes de Enfermedades , Pruebas Antimicrobianas de Difusión por Disco , Farmacorresistencia Bacteriana Múltiple , Gastroenteritis/epidemiología , Gastroenteritis/microbiología , Humanos , Pruebas de Sensibilidad Microbiana , Prevalencia , Federación de Rusia/epidemiología , Salmonella enterica/aislamiento & purificación , Salmonella enterica/metabolismo , beta-Lactamasas/biosíntesis , beta-Lactamasas/genéticaRESUMEN
In the last three decades, high rates of resistance to common first-line antimicrobial agents have been reported in Salmonella enterica serotype Typhi (Typhi), the causative organism of typhoid fever (TF), in many regions of the world, especially in South East Asia. Analysis of Typhi strains isolated from outbreaks and sporadic cases of TF in Son La province, northwest Vietnam, in 2002 revealed that 94.5% (85/90) of the isolates were fully susceptible to amoxicillin, chloramphenicol, cotrimoxazole, tetracycline, and nalidixic acid. There was a clear decline in the occurrence of multi-drug resistant (MDR) Typhi isolates collected in this province in 2002 (4.4%) compared with the period 1995-1999 in the same province (30.8-100%). By using molecular (IS200 profiling, PstI-ribotyping, XbaI-pulsed-field gel electrophoresis, and haplotyping) and phage-typing methods, we showed that the Typhi isolates from Son La province in 2002 were genetically related; however, they were unrelated to the previous MDR clones established in Vietnam.
Asunto(s)
Antibacterianos/farmacología , Salmonella typhi/efectos de los fármacos , Salmonella typhi/genética , Fiebre Tifoidea/microbiología , Adolescente , Adulto , Anciano , Tipificación de Bacteriófagos , Niño , Preescolar , Elementos Transponibles de ADN/genética , ADN Bacteriano/química , ADN Bacteriano/genética , Brotes de Enfermedades , Electroforesis en Gel de Campo Pulsado , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Pobreza , Ribotipificación , Salmonella typhi/crecimiento & desarrollo , Salmonella typhi/aislamiento & purificación , Fiebre Tifoidea/epidemiología , Vietnam/epidemiologíaRESUMEN
Infections by Shigella species are an important cause of diarrhoeal disease worldwide. Of 4198 Shigella isolates received by the French National Reference Centre for Escherichia coli and Shigella, 180 from patients with diarrhoea and dysentery in 2000-2004 did not react with any available polyclonal rabbit antisera used to identify the established Shigella serogroups. This study describes the molecular and phenotypic characteristics of these isolates in seroagglutination tests, molecular serotyping (rfb-RFLP and fliC-RFLP), ribotyping, detection of invasivity and enterotoxins genes, and antibiotic sensitivity. All isolates gave biochemical reactions typical of Shigella boydii, were mannitol-positive and indole-negative. They all carried invasion-associated genes, enterotoxin 2 [ShET-2] and an IS630 sequence. They had a unique ribotype that was distinct from all other Shigella and E. coli patterns. Further characterization by rfb-RFLP clearly distinguished this serogroup from all other Shigella or E. coli O-groups. The fliC-RFLP pattern corresponded to P4, an F-pattern which is associated with 10 different serogroups of S. boydii. A new antiserum prepared against strain 00-977 agglutinated all 180 isolates and cross-agglutination and absorption studies with anti-00-977 serum and anti-CDC 99-4528 (reference for the newly described S. boydii serogroup 20) serum showed identical antigenic structure. Furthermore, strains 00-977 and CDC 99-4528 had the same molecular serotype, ribotype and virulence genes.