RESUMEN
The study evoluated an in-house Spike Receptor Binding Domain Enzyme-Linked Immunosorbent Assay (RBD-IgG-ELISA) for detecting SARS-CoV-2 IgG antibodies in infected and vaccinated individuals. The assay demonstrated a sensitivity of 91%, specificity of 99.25%, and accuracy of 95.13%. Precision and reproducibility were highly consistent. The RBD-IgG-ELISA was able to detect 96.25% of Polymerase chain reaction (PCR) confirmed cases for SARS-CoV-2 infection, demonstrating positive and negative predictive values of 99,18% and 91,69%, respectively. In an epidemiological survey, ELISA, lateral flow immunochromatographic assay (LFIA), and electrochemiluminescence immunoassay (ECLIA) exhibited diagnostic sensitivities of 68.29%, 63.41%, and 70.73%, respectively, along with specificities of 82.93%, 80.49%, and 80.49%, respectively. Agreement between RBD-IgG-ELISA/PCR was moderate (k index 0.512). However, good agreement between different assays (RBD-IgG-ELISA/LFIA k index 0.875, RBD-IgG-ELISA/ECLIA k index 0.901). Test performance on individuals' samples were inferior due to seroconversion time and chronicity. The IgG-RBD-ELISA assay demonstrated its effectiveness in monitoring antibody levels among healthcare professionals, revealing significant differences both before and after the administration of the third vaccine dose, with heightened protection levels observed following the third dose in five Coronavirus disease (COVID-19) vaccine regimens. In conclusion, the RBD-IgG-ELISA exhibits high reproducibility, specificity, and sensitivity, making it a suitable assay validated for serosurveillance and for obtaining information about COVID-19 infections or vaccinations.
Asunto(s)
Anticuerpos Antivirales , Vacunas contra la COVID-19 , COVID-19 , Ensayo de Inmunoadsorción Enzimática , Inmunoglobulina G , SARS-CoV-2 , Glicoproteína de la Espiga del Coronavirus , Humanos , Ensayo de Inmunoadsorción Enzimática/métodos , COVID-19/diagnóstico , COVID-19/inmunología , COVID-19/prevención & control , SARS-CoV-2/inmunología , Inmunoglobulina G/sangre , Inmunoglobulina G/inmunología , Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/inmunología , Vacunas contra la COVID-19/inmunología , Glicoproteína de la Espiga del Coronavirus/inmunología , Reproducibilidad de los Resultados , Persona de Mediana Edad , Masculino , Femenino , Adulto , Prueba Serológica para COVID-19/métodos , Sensibilidad y Especificidad , Anciano , Vacunación , Adulto JovenRESUMEN
Noradrenaline (NE), the main neurotransmitter released by sympathetic nerve terminals, is known to modulate the immune response. However, the role of the sympathetic nervous system (SNS) on the development of autoimmune diseases is still unclear. Here, we report that the SNS limits the generation of pathogenic T cells and disease development in the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS). ß2-Adrenergic receptor (Adrb2) signaling limits T cell autoimmunity in EAE through a mechanism mediated by the suppression of IL-2, IFN-γ, and GM-CSF production via inducible cAMP early repressor (ICER). Accordingly, the lack of Adrb2 signaling in immune cells is sufficient to abrogate the suppressive effects of SNS activity, resulting in increased pathogenic T cell responses and EAE development. Collectively, these results uncover a suppressive role for the SNS in CNS autoimmunity while they identify potential targets for therapeutic intervention.
Asunto(s)
Encefalomielitis Autoinmune Experimental/inmunología , Inmunidad Celular , Esclerosis Múltiple/inmunología , Receptores Adrenérgicos beta 2/inmunología , Transducción de Señal/inmunología , Sistema Nervioso Simpático/inmunología , Linfocitos T/inmunología , Animales , Citocinas/genética , Citocinas/inmunología , Encefalomielitis Autoinmune Experimental/patología , Ratones , Ratones Noqueados , Esclerosis Múltiple/genética , Esclerosis Múltiple/patología , Receptores Adrenérgicos beta 2/genética , Transducción de Señal/genética , Sistema Nervioso Simpático/patología , Linfocitos T/patologíaRESUMEN
Despite accumulating evidence indicating that neurotransmitters released by the sympathetic nervous system can modulate the activity of innate immune cells, we still know very little about how norepinephrine impacts signaling pathways in dendritic cells (DC) and the consequence of that in DC-driven T cell differentiation. In this article, we demonstrate that ß2-adrenergic receptor (ß2AR) activation in LPS-stimulated DC does not impair their ability to promote T cell proliferation; however, it diminishes IL-12p70 secretion, leading to a shift in the IL-12p70/IL-23 ratio. Although ß2AR stimulation in DC induces protein kinase A-dependent cAMP-responsive element-binding protein phosphorylation, the effect of changing the profile of cytokines produced upon LPS challenge occurs in a protein kinase A-independent manner and, rather, is associated with inhibition of the NF-κB and AP-1 signaling pathways. Moreover, as a consequence of the inverted IL-12p70/IL-23 ratio following ß2AR stimulation, LPS-stimulated DC promoted the generation of CD4(+) T cells that, upon TCR engagement, produced lower amounts of IFN-γ and higher levels of IL-17. These findings provide new insights into molecular and cellular mechanisms by which ß2AR stimulation in murine DC can influence the generation of adaptive immune responses and may explain some aspects of how sympathetic nervous system activity can modulate immune function.
Asunto(s)
Linfocitos T CD4-Positivos/inmunología , Células Dendríticas/inmunología , Norepinefrina/inmunología , Receptores Adrenérgicos beta 2/inmunología , Transducción de Señal/inmunología , Animales , Western Blotting , Diferenciación Celular/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Citometría de Flujo , Activación de Linfocitos/inmunología , Ratones , Ratones Endogámicos C57BL , FN-kappa B/inmunología , Reacción en Cadena en Tiempo Real de la Polimerasa , Factor de Transcripción AP-1/inmunologíaRESUMEN
We have previously shown that regulatory T (Treg) cells that accumulate in the airways of allergic mice upregulate CC-chemokine receptor 4 (CCR4) expression. These Treg cells suppressed in vitro Th2 cell proliferation but not type 2 cytokine production. In the current study, using a well-established murine model of allergic lung disease or oral tolerance, we evaluated the in vivo activity of Treg cells in allergic airway inflammation with special focus on CCR4 function. We found that allergic, but not tolerant, mice treated with anti-CD25 Ab showed increased airway eosinophilia and IL-5- or IL-4-producing Th2 cells when compared with untreated mice. Notably, mice with CCR4 deficiency displayed an augmented airway allergic inflammation compared with wild-type or CCR2 knockout (KO) mice. The allergic phenotype of CCR4KO mice was similar to that observed in anti-CD25-treated mice. The exacerbated allergic inflammation of CCR4KO mice was directly associated with an impaired migration of Treg cells to airways and augmented frequency of pulmonary Th2 cells. Adoptive transfer of CD25(+)CD4(+) T cells expressing high levels of CCR4, but not CCR4KO CD25(+)CD4(+) T cells, attenuated the severe airway Th2 response of CCR4KO mice. Our results show that CCR4 is critically involved in the migration of Treg cells to allergic lungs that, in turn, attenuate airway Th2 activation and allergic eosinophilic inflammation.
Asunto(s)
Movimiento Celular/inmunología , Eosinofilia/inmunología , Neumonía/inmunología , Receptores CCR4/fisiología , Índice de Severidad de la Enfermedad , Linfocitos T Reguladores/inmunología , Animales , Eosinofilia/genética , Eosinofilia/patología , Femenino , Inflamación/genética , Inflamación/inmunología , Inflamación/patología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Neumonía/genética , Neumonía/patología , Receptores CCR4/deficiencia , Linfocitos T Reguladores/metabolismo , Linfocitos T Reguladores/patología , Células Th2/inmunología , Células Th2/metabolismo , Células Th2/patología , Regulación hacia Arriba/genética , Regulación hacia Arriba/inmunologíaRESUMEN
O objetivo deste estudo foi verificar os efeitos de cinco sessões consecutivas de exercício (natação) sobre o número de leucócitos totais, linfócitos e a concentração de citocinas circulantes. Foram utilizados ratos Wistar, ± 2 meses, peso ± 200 g, divididos em três grupos (n = 6): 1) controle sedentário, identificado pela sigla (C); 2) exercitado durante 5 sessões na mesma semana (5, 15, 30, 45 e 60 minutos por dia) em intensidade leve (sem sobrecarga adicional), identificado pela sigla (5SL); e 3) exercitado durante cinco sessões na mesma semana (5, 15, 30, 45 e 60 minutos por dia) em intensidade moderada (moderado = sobrecarga de 5% do peso corporal acoplado na região dorsal), identificado pela sigla (5SM). As análises realizadas foram: contagem de leucócitos, linfócitos circulantes, linfócitos dos linfonodos mesentéricos e concentração de citocinas através do método Elisa (IL-2, IL-6 e TNF-a). Aplicou-se o teste estatístico Anova, seguido de Post Hoc Tukey para análises múltiplas, com p £ 0.05. Foi Observado aumento nos leucócitos e linfócitos circulantes para os grupos exercitados em relação ao grupo sedentário; redução no número de linfócitos provenientes dos linfonodos mesentéricos nos grupos 5SL e 5SM em relação ao controle. Para as citocinas foi observada redução na TNF-a circulante nos grupos 5SL e 5SM; nenhuma alteração na IL-6 e IL-2. Concluiu-se que o exercício de volume crescente realizado por cinco sessões consecutivas, tanto na intensidade leve como na moderada, pode promover leucocitose, linfocitose e redução nos linfócitos teciduais. Adicionalmente, este tipo de exercício promove queda na concentração de TNF-a circulante.