Gout is a chronic disease. Gout symptoms are often experienced in the middle of the night. The onset of gout in the middle of the night is closely related to abnormal liver and gallbladder meridian. The purpose of this study was to investigate the clinical efficacy and possible mechanism of action of Tongbixiao pills in the treatment of hyperuricemia and gouty arthritis. The Tongbixiao pills we used included several traditional Chinese medicines, most of which tonify the spleen; strengthen the liver; benefit the kidney; and reduce heat, dampness, and blood stasis. In this randomized clinical study of 105 patients, we found that Tongbixiao pills can reduce uric acid levels in hyperuricemia patients. Additionally, the efficacy was similar to that of allopurinol and the level of uric acid did not increase significantly at eight weeks after withdrawal. In the absence of notable adverse reactions, Tongbixiao pills can also increase uric acid excretion, reduce serum creatinine and lipid levels, and reduce inflammation and relieve gout symptoms. In addition, we used SD rats to simulate gout and arthritis and divided them into five groups: normal group, model group, low-dose group, medium-dose group, and high-dose group. The inflammatory indices of the 40 SD rats were observed. After seven days, ankle swelling in rats in the treatment group was significantly reduced. The indices of uric acid, creatinine, and urea nitrogen in the treatment group were significantly lower than those in the model group, which proved that Tongbixiao pills could inhibit hyperuricemia in rats, thus treating gout. This study demonstrates that Tongbixiao pills can treat gout, provide more treatment options for gouty arthritis, and improve the quality of life of patients.
This study aims to isolate and identify the bacteria from the surface of Musca domestica adults in different livestock farms.The adults of Musca domestica were collected with sweep nets,then the surface of adult flies were washed with sterile water,and the eluted bacteria cultured with LB culture medium.The DNA of the bacteria isolated was extracted,16SrDNA fragment was amplified through general PCR,and the 16SrDNA sequence was blasted with bacteria sequence in NC-BI.Results showed that the number of Musca domestica adults and the bacteria from their surface had significant different in different livestock farms.The population density of Musca domestica adults had linear correlation with the number of bacteria from Musca domestica adults and the number of bacteria genus.Among the bacteria isolated from Musca domestica adults,Staphylococcus,Klebsiella,Escherichia and Serratia were the most common genus in the livestock farms.Most of the bacteria isolated from livestock farm house flies were opportunistic human bacterial pathogen,and the risk of bacterial pathogens spread to people was existed.The population density of house flies reflected the management level of different livestock farms.The house flies could mechanical transmit opportunistic human bacterial pathogen from livestock residue to human.So the house flies control and the monitoring of bacteria on body surface of house flies carried should be strengthened.
