Magnetic biochar serves as adsorbents and catalyst supports for the removal of antibiotics from wastewater: A review.

Numerous antibiotics are being released into the natural environment through wastewater. As antibiotic usage increases annually, its detrimental impact on the environment is escalating. Addressing environmental sustainability and human health requires significant attention towards antibiotic removal. In recent years, magnetic biochar (MBC) has gained widespread application in water treatment due to its exceptional adsorption and catalytic degradation capabilities. Antibiotics such as sulfamethoxazole (SMX), tetracycline (TC), ciprofloxacin (CIP), and others commonly exhibit an adsorption capacity by MBC ranging from 5 mg/g to 900 mg/g. Moreover, MBC typically removes over 90% of these antibiotics within 60 min. The effectiveness of antibiotic removal is significantly influenced by various preparation and modification methods. Furthermore, the incorporation of magnetism enables the material to be recycled and reused multiple times, thereby reducing consumption costs. This article discusses recent studies on antibiotic removal using MBC. It has been observed that variations in the selection of raw material and preparation procedures significantly affect antibiotic removal, while the mechanisms involved in antibiotic removal remain ambiguous. Additionally, it has been noted that the removal process may lead to secondary pollution and high preparation costs. Therefore, this review comprehensively outlines the utilization of MBC in the removal of antibiotics from wastewater, including aspects such as modification, preparation, removal mechanism, and factors influencing removal, and providing recommendations for antibiotic development. The aim is to offer researchers a clear understanding to advance the field of MBC materials.

Combined effects of oxytetracycline and microplastic on wheat seedling growth and associated rhizosphere bacterial communities and soil metabolite profiles.

The widespread application of antibiotics and plastic films in agriculture leads to new characteristics of soil pollution with the coexistence of antibiotics and microplastics. However, their combined effects on wheat seedling growth and associated rhizosphere bacterial communities and soil metabolite profiles remain unclear. Here, in the potted experiment, wheat was treated with individual oxytetracycline (0, 5.0, 50.0, and 150.0 mg kg-1) and the combination of oxytetracycline and polyethylene microplastic (0.2%). Results showed that 150 mg kg-1 oxytetracycline combined with microplastic significantly reduced the biomass and height of the plant. Compared with CK, all the treatments exposed to the combination of oxytetracycline and polyethylene microplastic significantly promoted carotenoid content and peroxidase activity in wheat leaves. Soil dehydrogenase and urease activities were more sensitive to current pollutant exposure than sucrase activity. Oxytetracycline (150 mg kg-1) alone and in combination with polyethylene significantly decreased the abundances of certain genera belonging to plant growth-promoting rhizobacteria (PGPR) in soil, such as Arthrobacter, Gemmatimonas, Massilia, and Sphingomonas. Combined exposure of 150 mg kg-1 oxytetracycline and polyethylene microplastic significantly altered multiple metabolites including organic acids and sugars. Network analysis indicated that co-exposure of 150 mg kg-1 oxytetracycline and microplastic may affect the colonization and succession of PGPR by regulating soil metabolites, thereby indirectly inhibiting wheat seedling growth. The results help to elucidate the potential mechanisms of phytotoxicity of the combination of oxytetracycline and polyethylene microplastic.

Stress response to oxytetracycline and microplastic-polyethylene in wheat (Triticum aestivum L.) during seed germination and seedling growth stages.

Much efforts have been devoted to clarify the phytotoxicity of individual contaminants in plants, such as individual antibiotic and microplastic; however, little is known about the phytotoxicity of their combined exposure. Here, we investigated the effects of individual and combined exposure of wheat (Triticum aestivum L.) (Xiaoyan 22) to oxytetracycline (OTC) and polyethylene (PE) microplastics using physiological and metabolic profilings. During the seed germination stage, OTC induced phytotoxicity, as observed through the changes of root elongation, sprout length, fresh weight and the vitality index, with significant effect at the 50 and 150 mg·L-1 levels; the effect of PE microplastics depended on the OTC level in the combined exposure groups. During seedling cultivation, catalase (CAT) and ascorbate peroxidase (APX), as antioxidant enzyme indices, were sensitive to OTC exposure stress, although OTC was not determined in leaves. Untargeted metabolomics of wheat leaves revealed OTC concentration-, metabolite class- and PE-dependent metabolic responses. Dominant metabolites included carboxylic acids, alcohols, and amines in the control group and all treatment groups. Compared to only OTC treatment, PE reprogrammed carboxylic acid and alcohol profiles in combined exposure groups with obvious separation in PLS-DA. Combined exposure induced fewer metabolites than OTC exposure alone at the 5 and 50 mg·L-1 levels. The shared metabolite numbers were higher in the OTC groups than in the PE-OTC groups. Pathway enrichment analysis showed a drift in metabolic pathways between individual and combined exposure to OTC and PE, which included glyoxylate and dicarboxylate metabolism, amino acid metabolism and isoquinoline alkaloid biosynthesis. Among metabolites, aromatic acids and amino acids were more sensitive to combined exposure than individual exposure. These results contribute to clarifying the underlying mechanisms of phytotoxicity of individual and combined exposure to OTC and PE.

Prochloraz alone or in combination with nano-CuO promotes the conjugative transfer of antibiotic resistance genes between Escherichia coli in pure water.

Conjugative plasmid transfer is a major contributor to the spread of antibiotic resistance genes (ARGs). However, the role of conventional fungicides on conjugative plasmid transfer has been neglected. Based on the condition that the increasing use of the combination of nano- and conventional fungicides will lead to combined contamination, the effects of a conventional fungicide prochloraz alone or in combination with nano-CuO on the conjugation of plasmid RP4 between Escherichia coli in phosphate-buffered saline were investigated in this study. The results demonstrated that 50 µg/L prochloraz alone significantly increased the conjugative transfer by 1.82 folds. The combination of 100 µg/L nano-CuO and prochloraz at 5, 50, and 500 µg/L significantly increased the conjugation by 2.56, 3.61, and 2.13 folds, respectively. The promotion of conjugative transfer of ARGs mediated by fungicides is mainly attributed to (i) the increased cell membrane permeability, (ii) the increased cell adhesion via enhancing the synthesis of polysaccharides in extracellular polymeric substances, and (iii) the up-regulation of the genes relevant to conjugation, oxidative stress, SOS response, outer membrane, polysaccharide export, intercellular adhesion, and ATP synthesis. Our findings provide evidence for the contribution of fungicides to ARGs transfer, which is significant to control the risk of ARGs dissemination.

Linkage of antibiotic resistance genes, associated bacteria communities and metabolites in the wheat rhizosphere from chlorpyrifos-contaminated soil.

Rhizosphere is a crucial site for the proliferation of antibiotic resistance genes (ARGs) in agricultural soil. Pesticide contamination is ubiquitous in soil, such as chlorpyrifos as one of the most commonly used pesticides. However, limited knowledge is reported about ARGs profiles changes and the driving mechanism of ARGs prevalence in rhizosphere soil after adding pesticide. In this study, irrespective of chlorpyrifos presence, the abundances of ARGs (tetM, tetO, tetQ, tetW, tetX, sul1 and sul2) and intI1 in rhizosphere soil of wheat were obviously higher than those in bulk soil. 20.0 mg·kg-1 chlorpyrifos significantly increased the abundance of total ARGs and intI1 in bulk soil, respectively, at day 50 and 100, but not in rhizosphere soil. Rhizosphere influence on ARGs was far greater than chlorpyrifos. ARGs and intI1 abundances were higher at day 50 than ones at day 100. C/N ratio and NO3--N content, which were affected by rhizosphere and cultivation time, significantly explained the increased ARGs. Compared to bulk soil, rhizosphere shifted host bacteria of tetracycline resistance genes (TRGs), intI1 at genus level, and host bacteria of sul1, sul2 at phylum level. Rhizosphere simplified the linkage of ARGs, host bacteria and metabolites. Bacterial communities played important roles in the variation of ARGs and intI1, and the difference in the distribution of potential hosts between bulk and rhizosphere soil was related to metabolites abundance and composition. These results provide valuable information for understanding the linkage of ARGs, associated bacteria communities and metabolites in the wheat rhizosphere soil.

Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.

The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed. The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground. The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days. Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment. Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment. However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous. Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure. NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity. Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD). As a result, the above factors were related to the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae at the phylum level. After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle. The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure. Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar. Network analysis showed that the influence of soil bacterial community on metabolites varied with metabolites and bacteria species. The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure. Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles. These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.

Citric acid enhances Ce uptake and accumulation in rice seedlings exposed to CeO2 nanoparticles and iron plaque attenuates the enhancement.

To assess the role of citric acid, as a typical low-molecular-weight organic acid from root exudates, on cerium (Ce) uptake, accumulation and translocation in rice seedlings (Oryza sativa L.) exposed to two CeO2 nanoparticles (NPs) (14 nm and 25 nm). A hydroponic experiment was performed under two citric acid levels (0.01 and 0.04 mmol L-1) combined with iron plaque presence. Citric acid significantly enhanced surface-Ce, root-Ce and shoot-Ce accumulation, irrespective of NPs size and iron plaque presence. The increased surface-Ce was associated with the promoted interactive attraction between NPs and root surface, and the enhanced NPs dissolution. Surface-Ce (containing crystalline and amorphous fractions of iron plaque) accumulation increased with the increase of citric acid concentrations. However, the enhancement influence of 0.01 mmol L-1 citric acid on root-Ce, shoot-Ce accumulations, rice-Ce distribution and TFroot-shoot was more remarkable than citric acid (0.04 mmol L-1), which suggested higher food security risk for human health with environment-level citric acid. Iron plaque presence attenuated the enhancement effect of citric acid on rice-Ce accumulation and distribution (containing surface-Ce, root-Ce and shoot-Ce) due to the reduced attractive interaction between NPs and root surface from the effect of Fe2+ being dissolved by iron plaque. Above effect of citric acid and iron plaque was more remarkable in 25 nm NP than 14 nm NP.

Citric acid and glycine reduce the uptake and accumulation of Fe2O3 nanoparticles and oxytetracycline in rice seedlings upon individual and combined exposure.

Uptake of nanoparticles and antibiotics by plants is root exudates-dependent, however, the underlying influence processes and mechanisms from different root exudates are rarely investigated. A hydroponic experiment was conducted to investigate the accumulation of Fe2O3 nanoparticle (NP) and oxytetracycline (OTC) in rice seedlings, in the absence or presence of citric acid or glycine, acting as components of root exudates. Irrespective of individual or combined exposure of Fe2O3 NP and OTC, citric acid and glycine both reduced surface-Fe, surface-OTC, root-OTC, shoot-OTC accumulations with dose-effect relationship. Two exudates increased |ζ| values of NP, which weakened the interactive attraction between NP and root surface and then decreased surface-Fe accumulation. Citric acid and glycine binding with OTC in solution decreased surface-OTC accumulation, and further decreased root-OTC and shoot-OTC accumulations. Combined exposure of two pollutants alleviated the reduction effect of citric acid and glycine on surface-Fe/surface-OTC/root-OTC accumulations due to their high accumulations in combined exposure compared to individual exposure. Although citric acid and glycine promoted TFroot-shoot and TFsurface-root of two pollutants, respectively, they always decreased total rice-Fe and rice-OTC accumulations. Therefore, the presence of root exudates decreased the bioaccumulation of Fe2O3 NP and OTC in rice upon their individual and combined exposure through changing their environmental behaviors in rhizosphere.

Fate of antibiotic resistance genes and mobile genetic elements during anaerobic co-digestion of Chinese medicinal herbal residues and swine manure.

Swine manure is an important reservoir for antibiotic resistance genes (ARGs) but anaerobic co-digestion (AcoD) can potentially reduce the abundance of these ARGs. However, few studies have considered the effects of Chinese medicinal herbal residues (CMHRs) on the variations in ARGs and mobile genetic elements (MGEs) during AcoD. Thus, this study explored the fate of ARGs and MGEs during the AcoD of CMHRs and swine manure. The results showed that CMHRs effectively reduced the abundances of the main ARGs (excluding ermF, qnrA, and tetW) and four MGEs (by 36.7-96.5%) after AcoD. Redundancy analysis showed that changes in the bacterial community mainly affected the fate of ARGs rather than horizontal gene transfer by MGEs. Network analysis indicated that 17 bacterial genera were possible hosts of ARGs. The results of this study suggest that AcoD with CMHRs could be employed to remove some ARGs and MGEs from swine manure.

Influence of combined sulfachloropyridazine sodium and zinc on enzyme activities and biogas production during anaerobic digestion of swine manure.

In order to study the influence of different concentrations of zinc and sulfachloropyridazine sodium (SCPS) on anaerobic digestion (AD) during biogas production, we determined the levels of urease, dehydrogenase activity, and volatile fatty acids (VFAs) in batch tests. The experiments were conducted in small AD devices at a temperature of 37 °C using swine manure and wheat straw as raw materials. Four digestion trials were performed using different zinc and SCPS contents: control digestion with no additives (CK), SCPS at 630 mg kg-1 dry weight (S), SCPS at 630 mg kg-1 with zinc at 500 mg kg-1 dry weight (SL), and SCPS at 630 mg kg-1 with zinc at 5,000 mg kg-1 dry weight (SH). The biogas accumulation under S was 1.7 times that with CK, while SL and SH produced 78% and 35% of that under S, respectively. Correlation analysis showed that the accumulated biogas was significantly negatively correlated (p < 0.05) with VFAs, and the urease activity was significantly negatively correlated (p < 0.01) with zinc and significantly positively correlated with VFAs (p < 0.05). The dehydrogenase activity was strongly correlated (p < 0.01) with the biogas accumulated during the AD of swine manure.

Effects of copper on the composition and diversity of microbial communities in laboratory-scale swine manure composting.

This study investigated the effects of adding copper at 3 treatment levels (0 (control: CK), 200 (low: L), and 2000 (high: H) mg·kg-1 treatments) on the bacterial communities during swine manure composting. The abundances of the bacteria were determined by quantitative PCR and their compositions were evaluated by high-throughput sequencing. The results showed that the abundance of bacteria was inhibited by the H treatment during days 7-35, and principal component analysis clearly separated the H treatment from the CK and L treatments. Actinobacteria, Firmicutes, and Proteobacteria were the dominant bacterial taxa, and a high copper concentration decreased the abundances of bacteria that degrade cellulose and lignin (e.g., class Bacilli and genus Truepera), especially in the mesophilic and thermophilic phases. Moreover, network analysis showed that copper might alter the co-occurrence patterns of bacterial communities by changing the properties of the networks and the keystone taxa, and increase the competition by increasing negative associations between bacteria during composting. Temperature, water-soluble carbohydrates, and copper significantly affected the variations in the bacterial community according to redundancy analysis. The copper content mainly contributed to the bacterial community in the thermophilic and cooling phases, where it had positive relationships with potentially pathogenic bacteria (e.g., Corynebacterium_1 and Acinetobacter).

Prevalence of quinolone resistance genes, copper resistance genes, and the bacterial communities in a soil-ryegrass system co-polluted with copper and ciprofloxacin.

The presence of high concentrations of residual antibiotics and antibiotic resistance genes (ARGs) in soil may pose potential health and environmental risks. This study investigated the prevalence of plasmid-mediated quinolone resistance (PMQR) genes, copper resistance genes (CRGs), and the bacterial communities in a soil-ryegrass pot system co-polluted with copper and ciprofloxacin (CIP; 0, 20, or 80 mg kg-1 dry soil). Compared with the samples on day 0, the total relative abundances of the PMQR genes and mobile genetic elements (MGEs) were reduced significantly by 80-89% in the ryegrass and soil by the cutting stage (after 75 days). The abundances of PMQR genes and MGEs were reduced by 63-81% in soil treated with 20 mg kg-1 CIP compared with the other treatments, but the abundances of CRGs increased by 18-42%. The presence of 80 mg kg-1 CIP affected the microbial community structure in the soil by increasing the abundances of Acidobacteria and Thaumarchaeota, but decreasing those of Firmicutes. Redundancy analysis indicated that the pH and microbial composition were the main factors that affected the variations in PMQR genes, MGEs, and CRGs, where they could explain 42.2% and 33.3% of the variation, respectively. Furthermore, intI2 may play an important role in the transfer of ARGs. We found that 80 mg kg-1 CIP could increase the abundances of ARGs and CRGs in a soil-ryegrass pot system.

Effects of superabsorbent polymers on the abundances of antibiotic resistance genes, mobile genetic elements, and the bacterial community during swine manure composting.

Superabsorbent polymers (SAPs) are considered suitable amendments for reducing the selection pressure due to heavy metals and the abundances of antibiotic resistance genes (ARGs) during composting. In this study, three SAP (sodium polyacrylate) levels (0, 5, and 15mgkg-1 of compost) were applied and their effects on the abundances of ARGs, mobile genetic elements (MGEs), and the bacterial community were investigated. After composting, the abundances of ARGs and MGEs decreased to different extent, where the removal efficiencies for tetW, dfrA7, ermX, aac(6')-ib-cr and MGEs exceeded 90%. The high SAP concentration significantly reduced the abundances of ARGs and MGEs, and changed the microbial community. Redundancy analysis indicated that the moisture content mainly explained the changes in ARGs and MGEs. Network analysis determined the potential hosts of ARGs and MGEs, and their co-occurrence. The results suggested that applying 15mgkg-1 SAP is appropriate for reducing ARGs in compost.

Behavior of antibiotic resistance genes during co-composting of swine manure with Chinese medicinal herbal residues.

Swine manure is considered to be a reservoir for antibiotic resistance genes (ARGs) but little is known about the variations in ARGs during the co-composting of swine manure with Chinese medicinal herbal residues (CMHRs). Thus, this study explored the effects of CMHRs on the variations in ARGs during co-composting with swine manure. The results showed that CMHRs could reduce effectively most of the targeted ARGs (0.18-2.82logs) and mobile genetic elements (MGEs) (0.47-3.34logs). The correlations indicated that CMHRs might decrease the spread of ARGs via horizontal gene transfer. Redundancy analysis showed that the bacterial communities had more important effects on the variations in ARGs compared with environmental factors and MGEs. The results of this study demonstrate that CMHRs can decrease the abundances of ARGs and MGEs, as well as reducing the risk of ARGs spreading during the application of compost products to farmland.

Mechanisms and effects of arsanilic acid on antibiotic resistance genes and microbial communities during pig manure digestion.

High concentrations of residual arsanilic acid occur in pig manure due to its use in feed to promote growth and control diseases. This study compared the effects of arsanilic acid at three concentrations (0, 325, and 650mg/kg dry pig manure) on the abundance of antibiotic resistance genes (ARGs) and the microbial community during anaerobic digestion. Addition of 650mg/kg arsanilic acid enhanced the absolute abundances of tetC, sul2, ermB, and gyrA more than twofold in the digestion product. Redundancy analysis indicated that the change in the microbial community structure was the main driver of variation in the ARGs profile. The As resistance gene arsC co-occurred with four ARGs and intI1, possibly causing the increase in ARGs under pressure by arsanilic acid. High arsanilic acid concentrations can increase the risk of ARGs occurring in anaerobic digestion products. The amount of arsanilic acid used as a feed additive should be controlled.

Isolation of new polyketide synthase gene fragments and a partial gene cluster from East China Sea and function analysis of a new acyltransferase.

Using the consensus-degenerate hybrid oligonucleotide primer polymerase chain reaction method, 26 new ketoacyl synthase (KS) fragments were isolated from a marine sediment sample in the East China Sea (ECS) and analyzed by construction of a phylogenetic tree. With a digoxigenin-labeled KS gene fragment used as a probe, a partial polyketide synthase (PKS) gene cluster was isolated and identified by hybridization screening of a marine sediment sample metagenome fosmid library constructed for this study. A new acyltransferase (AT) gene was cloned from the PKS gene cluster and heterogeneously expressed as a protein fused to maltose-binding protein (MBP). Ultraviolet spectrophotometry was used to study the binding of the MBP-AT fusion protein and single AT domain to substrates using MBP and bovine serum albumin as control proteins. Binding constants (Ka, per micromolar) were calculated and used to analyze the substrate specificity of the acyltransferase. We concluded that there are many unrevealed new PKS gene clusters in marine sediments in the ECS. The acyltransferase is presumably an acetyltransferase from a new PKS gene cluster.