A novel multi-task learning network for skin lesion classification based on multi-modal clues and label-level fusion.

In this paper, we propose a multi-task learning (MTL) network based on the label-level fusion of metadata and hand-crafted features by unsupervised clustering to generate new clustering labels as an optimization goal. We propose a MTL module (MTLM) that incorporates an attention mechanism to enable the model to learn more integrated, variable information. We propose a dynamic strategy to adjust the loss weights of different tasks, and trade off the contributions of multiple branches. Instead of feature-level fusion, we propose label-level fusion and combine the results of our proposed MTLM with the results of the image classification network to achieve better lesion prediction on multiple dermatological datasets. We verify the effectiveness of the proposed model by quantitative and qualitative measures. The MTL network using multi-modal clues and label-level fusion can yield the significant performance improvement for skin lesion classification.

Preparation and evaluation of dual drug-loaded nanofiber membranes based on coaxial electrostatic spinning technology.

Wound healing is a complicated process consisting of wound bleeding, inflammatory response, cell proliferation and tissue remodeling. During this long-term period, wound is vulnerable to infection by bacteria or microbes. Therefore, we prepared a novel centella total glucoside-ciprofloxacin dual-loaded coaxial nanofiber membrane (CDCNM) by using coaxial electrostatic spinning technique. To satisfy personalized therapeutic demands by adjusting release behaviors, we loaded centella total glucoside (CTG) and ciprofloxacin (CIP) into different positions of the fibers and the morphology and coaxial structure of the nanofiber membranes were analyzed by SEM and TEM. In addition, water contact angle, water absorption capacity, breathability and in vitro drug release were tested. In vitro cell experiments demonstrated that CDCNM can promote fibroblast proliferation. CDCNM demonstrated excellent antimicrobial activity through the agar flat dish diffusion method. Furthermore, rat scald experiments showed that CDCNM significantly accelerated scald healing, meanwhile immunohistochemical staining showed that CDCNM promoted the expression of CD31 and VEGF during early wound healing, which accelerated scald healing by promoting neovascularization and endothelial cell proliferation. As a topical multifunctional wound dressing, this dual drug-loaded nanofiber membrane achieved scald healing effect and continuous bacterial inhibition, which provides new ideas for existing trauma treatment tools and dual drug delivery systems.

Design of ROS-Responsive Hyaluronic Acid-Methotrexate Conjugates for Synergistic Chemo-Photothermal Therapy for Cancer.

Combining chemotherapy with photothermal therapy (PTT) for cancer treatment could overcome the inherent limitations of both single-modality chemotherapy and PTT. However, the obstacle of accurate drug delivery to tumor sites based on chemo-photothermal remains challenging. This article describes development of a reactive oxygen species (ROS)-responsive hyaluronic acid-based nanoparticle to overcome these drawbacks. Herein, HA-TK-MTX (HTM) was synthesized by a ROS-responsive cleaved thioketal moiety linker (TK) of methotrexate (MTX) and hyaluronic acid (HA). Through hydrophobic interaction and π-π stacking interaction, a photothermal agent IR780 was integrated into the HTM, and the IR780/HTM nanoparticles (IHTM NPs) were obtained. The IHTM NPs show high photostability, excellent photothermal performance, remarkable tumor-targeting ability, and ROS sensibility. Due to the accurate drug delivery ability and superior chemo-photothermal treatment effect of IHTM NPs, the tumor inhibition rate reached 70.95% for 4T1 tumor-bearing mice. This work serves as a precedent for the chemo-photothermal therapy of cancer by rationally designing ROS-responsive nanoparticles.

Expression and characterization of a novel lytic polysaccharide monooxygenase, PdLPMO9A, from the edible fungus Pleurotus djamor and its synergistic interactions with cellulase in corn straw biomass saccharification.

Lytic polysaccharide monooxygenases play a unique role in biomass saccharification. A novel gene, PdLPMO9A, from Pleurotus djamor, was reported to be upregulated during the degradation of corn straw in our previous research. However, very little information is available on PdLPMO9A. Therefore, PdLPMO9A was heterologously expressed in Pichia pastoris, and biophysical characterisitics of the recombinant protein PdLPMO9A were investigated; it was shown to have superior thermostability and pH stability. PdLPMO9A markedly improved the cellulase-mediated saccharification of corn straw, when the dosage of PdLPMO9A was 0.66 mg/g corn straw and hydrolysis time was 48 h. When CuSO4 was added at a concentration of 0.1 mM, glucose yield rose by a further 28.16%. In light of these findings, it was concluded that PdLPMO9A has the potential to function as an essential component of a cellulase cocktail capable of ensuring the saccharification of corn straw biomass.

Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.

Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit. However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization. In this work, SLS polysaccharide (SP) was ultrasonically extracted by optimizing the process conditions with response surface methodology. Using gradient ethanol precipitation, SP was separated into SP40, SP60 and SP80, and their monosaccharide composition, structural properties, and antioxidant potential were further characterized and compared. The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide. These fractions also varied in the surface morphology, where SP80 was looser and more porous than SP40 and SP60. Furthermore, SP and SP80 displayed the strongest antioxidant activities in vitro. This study identified a novel and practical strategy to valorize SLS for valuable polysaccharide.