Background: Extracorporeal membrane oxygenation (ECMO) has recently emerged as a critical support system for lung function in patients awaiting lung transplantation. This meta-analysis investigates the prognostic factors of lung transplantation following ECMO bridging therapy. Methods: A comprehensive search was conducted in PubMed, Cochrane Library, Embase, CINAHL, Web of Science, Scopus, and ProQuest databases from inception to August 11, 2023. Included were cohort or case-control studies focusing on prognostic factors of lung transplantation with ECMO bridging therapy. Data extraction was performed independently, and study quality was assessed. A meta-analysis was carried out using RevMan 5.4 and Stata17.0 software to aggregate mortality rates and pertinent prognostic factors of ECMO as a bridge to lung transplantation. Results: The search identified eight trials encompassing 1,086 participants. The prognosis of patients undergoing lung transplantation with ECMO bridging was significantly associated with several factors: prolonged ECMO support [odds ratio 1.07, 95% confidence interval (CI): 1.02-1.12, I2=77%], deterioration in liver and kidney function (odds ratio 3.62, 95% CI: 2.37-5.54, I2=0%), and complications during ECMO (odds ratio 2.24, 95% CI: 1.45-3.44, I2=5%). Conclusions: Prolonged ECMO support, declining liver and kidney functions, and complications during ECMO are vital prognostic factors in lung transplantation following ECMO bridging therapy.
BACKGROUND: Droplet digital PCR (ddPCR) is increasingly used in diagnosing clinical pathogens, but its effectiveness in cirrhosis patients with suspected ascites infection remains uncertain. METHODS: The diagnostic performance of ddPCR was assessed in 305 ascites samples, utilizing culture and clinical composite standards. The quantitative value and potential clinical impact of ddPCR were further analyzed in patients with spontaneous bacterial peritonitis. RESULTS: With culture standards, ddPCR demonstrated a sensitivity of 86.5% and specificity of 83.2% for bacterial or fungal detection. After adjustment of clinical composite criteria, specificity increased to 96.4%. Better diagnostic performance for all types of targeted pathogens, particularly fungi, was observed with ddPCR compared to culture, and more polymicrobial infections were detected (30.4% versus 5.7%, p < 0.001). Pathogen loads detected by ddPCR correlated with white blood cell count in ascites and blood, as well as polymorphonuclear cell (PMN) count in ascites, reflecting infection status rapidly. A positive clinical impact of 55.8% (43/77) was observed for ddPCR, which was more significant among patients with PMN count ≤ 250/mm3 in terms of medication adjustment and new diagnosis. ddPCR results for fungal detection were confirmed by clinical symptoms and other microbiological tests, which could guide antifungal therapy and reduce the risk of short-term mortality. CONCLUSIONS: ddPCR, with appropriate panel design, has advantages in pathogen detection and clinical management of ascites infection, especially for patients with fungal and polymicrobial infections. Patients with atypical spontaneous bacterial peritonitis benefited more from ddPCR.
INTRODUCTION: The widespread application of extracorporeal membrane oxygenation (ECMO) has enhanced clinical outcomes for patients experiencing cardiac arrest. However, its effectiveness is still limited and falls short of the desired level. Therapeutic hypothermia, which maintains body temperatures between 32°C and 36°C in cardiac arrest patients treated with ECMO, has been proposed as a potential means of neuroprotection and increased survival rates. Nevertheless, it remains controversial, and its impact on patient complications has yet to be fully understood. Thus, this paper aims to update the protocol for a systematic review of patients treated with ECMO and therapeutic hypothermia, in order to explore its effects on survival and neurological function. METHOD AND ANALYSIS: This protocol has been developed in compliance with the Preferred Reporting Items for Systematic Review and Meta-analysis Protocols 2015. The following databases will be systematically searched: PubMed, Web of Science, Cochrane Library, Embase, Ovid, CNKI, Wanfang and China Biology Medicine Disc. The database search strategy will use a combination of subject terms and free-text keywords. The search will encompass articles from the inception of each database up to 15 June 2023. Inclusion criteria encompass randomised controlled trials, cohort studies, case-control studies and quasi-experimental studies. Two researchers will independently review articles and extract relevant data based on these criteria. Any disagreements will be resolved through discussion. Data analysis will be performed using Review Manager software. ETHICS AND DISSEMINATION: Since no patient data were collected in this study, ethical approval was not required. Research findings will be released in a peer-reviewed journal. PROSPERO REGISTRATION NUMBER: CRD42023435353.
Extracorporeal Membrane Oxygenation , Heart Arrest , Hypothermia, Induced , Humans , Extracorporeal Membrane Oxygenation/methods , Systematic Reviews as Topic , Meta-Analysis as Topic , Hypothermia, Induced/methods , Research Design
Instance segmentation plays an important role in the automatic diagnosis of cervical cancer. Although deep learning-based instance segmentation methods can achieve outstanding performance, they need large amounts of labeled data. This results in a huge consumption of manpower and material resources. To solve this problem, we propose an unsupervised cervical cell instance segmentation method based on human visual simulation, named HVS-Unsup. Our method simulates the process of human cell recognition and incorporates prior knowledge of cervical cells. Specifically, firstly, we utilize prior knowledge to generate three types of pseudo labels for cervical cells. In this way, the unsupervised instance segmentation is transformed to a supervised task. Secondly, we design a Nucleus Enhanced Module (NEM) and a Mask-Assisted Segmentation module (MAS) to address problems of cell overlapping, adhesion, and even scenarios involving visually indistinguishable cases. NEM can accurately locate the nuclei by the nuclei attention feature maps generated by point-level pseudo labels, and MAS can reduce the interference from impurities by updating the weight of the shallow network through the dice loss. Next, we propose a Category-Wise droploss (CW-droploss) to reduce cell omissions in lower-contrast images. Finally, we employ an iterative self-training strategy to rectify mislabeled instances. Experimental results on our dataset MS-cellSeg, the public datasets Cx22 and ISBI2015 demonstrate that HVS-Unsup outperforms existing mainstream unsupervised cervical cell segmentation methods.
Uterine Cervical Neoplasms , Humans , Female , Computer Simulation , Uterine Cervical Neoplasms/diagnostic imaging , Image Processing, Computer-Assisted
BACKGROUND: Prolonged invasive respiratory support and extracorporeal membrane oxygenation (ECMO) in patients requiring urgent lung transplantation (ULTx) present significant challenges to clinical practice due to severe underlying diseases and complex conditions. The aim of the study was to report the clinical outcomes of patients who received ULTx and followed the perioperative rehabilitation protocol implemented in a lung transplant center. METHODS: A retrospective analysis was conducted in ULTx patients who required preoperative invasive mechanical ventilation (IMV) and ECMO between January 2018 and January 2023. Data were retrieved from electronic medical records at our lung transplant center. RESULTS: Fourteen patients (mean age 57.43±10.97 years; 12 males, 2 females) underwent ULTx with bridging ECMO and IMV. The mean body mass index was 23.94±3.33 kg/m², and the mean Acute Physiology and Chronic Health Evaluation (APACHE) II score was 21.50±3.96. The Nutritional Risk Screening 2002 (NRS 2002) scores were ≥3. ULTx was performed after an 8.5-day waiting period (interquartile interval [IQR] 5.0-26.5 d). Following the surgeries, the average lengths of ECMO and IMV were 1.0 (IQR 1.0-2.0) d and 5.0 (IQR 3.0-7.3) d, respectively. The total length of hospital stay was 60.1±30.8 d, with an average intensive care unit stay of 38.3±22.9 d and post-operative hospitalization stay of 45.8±26.1 d. Two patients died within 30 d after ULTx, with a 30-day survival rate of 85.71%. CONCLUSION: Patients receiving ULTx showed an acceptable short-term survival rate, validating the practicality and safety of the treatment protocols implemented in our center.
This paper considers a class of multi-agent distributed convex optimization with a common set of constraints and provides several continuous-time neurodynamic approaches. In problem transformation, l1 and l2 penalty methods are used respectively to cast the linear consensus constraint into the objective function, which avoids introducing auxiliary variables and only involves information exchange among primal variables in the process of solving the problem. For nonsmooth cost functions, two differential inclusions with projection operator are proposed. Without convexity of the differential inclusions, the asymptotic behavior and convergence properties are explored. For smooth cost functions, by harnessing the smoothness of l2 penalty function, finite- and fixed-time convergent algorithms are provided via a specifically designed average consensus estimator. Finally, several numerical examples in the multi-agent simulation environment are conducted to illustrate the effectiveness of the proposed neurodynamic approaches.
Algorithms , Neural Networks, Computer , Computer Simulation , Consensus
Supported metal clusters comprising of well-tailored low-nuclearity heteroatoms have great potentials in catalysis owing to the maximized exposure of active sites and metal synergy. However, atomically precise design of these architectures is still challenging for the lack of practical approaches. Here, we report a defect-driven nanostructuring strategy through combining defect engineering of nitrogen-doped carbons and sequential metal depositions to prepare a series of Pt and Mo ensembles ranging from single atoms to sub-nanoclusters. When applied in continuous gas-phase decomposition of formic acid, the low-nuclearity ensembles with unique Pt3Mo1N3 configuration deliver high-purity hydrogen at full conversion with unexpected high activity of 0.62 molHCOOH molPt-1 s-1 and remarkable stability, significantly outperforming the previously reported catalysts. The remarkable performance is rationalized by a joint operando dual-beam Fourier transformed infrared spectroscopy and density functional theory modeling study, pointing to the Pt-Mo synergy in creating a new reaction path for consecutive HCOOH dissociations.
Electrocatalytic water splitting powered by sustainable electricity is a crucial approach for the development of new generation green hydrogen technology. Biomass materials are abundant and renewable, and the application of catalysis can increase the value of some biomass waste and turn waste into fortune. Converting economical and resource-rich biomass into carbon-based multicomponent integrated catalysts (MICs) has been considered as one of the most promising ways to obtain inexpensive, renewable and sustainable electrocatalysts in recent years. In this review, recent advances in biomass-derived carbon-based MICs towards electrocatalytic water splitting are summarized, and the existing issues and key aspects in the development of these electrocatalysts are also discussed and prospected. The application of biomass-derived carbon-based materials will bring some new opportunities in the fields of energy, environment, and catalysis, as well as promote the commercialization of new nanocatalysts in the near future.
During homeostasis and after injury, adult muscle stem cells (MuSCs) activate to mediate muscle regeneration. However, much remains unclear regarding the heterogeneous capacity of MuSCs for self-renewal and regeneration. Here, we show that Lin28a is expressed in embryonic limb bud muscle progenitors, and that a rare reserve subset of Lin28a+Pax7- skeletal MuSCs can respond to injury at adult stage by replenishing the Pax7+ MuSC pool to drive muscle regeneration. Compared with adult Pax7+ MuSCs, Lin28a+ MuSCs displayed enhanced myogenic potency in vitro and in vivo upon transplantation. The epigenome of adult Lin28a+ MuSCs showed resemblance to embryonic muscle progenitors. In addition, RNA-sequencing revealed that Lin28a+ MuSCs co-expressed higher levels of certain embryonic limb bud transcription factors, telomerase components and the p53 inhibitor Mdm4, and lower levels of myogenic differentiation markers compared to adult Pax7+ MuSCs, resulting in enhanced self-renewal and stress-response signatures. Functionally, conditional ablation and induction of Lin28a+ MuSCs in adult mice revealed that these cells are necessary and sufficient for efficient muscle regeneration. Together, our findings connect the embryonic factor Lin28a to adult stem cell self-renewal and juvenile regeneration.
Adult Stem Cells , Satellite Cells, Skeletal Muscle , Animals , Mice , Muscle, Skeletal , Muscle Fibers, Skeletal , Cell Self Renewal
BACKGROUND: Multiple myeloma (MM) is a common blood system malignance accompanied by monoclonal plasma cell hyperplasia. Homeobox C6 (HOXC6) acts as an oncogene in various cancers, but its function on MM is elusive. OBJECTIVE: The role of HOXC6 on MM development was clarified in this study. METHODS: HOXC6 expression and its clinical significance were determined in the peripheral blood samples collected from forty MM patients and thirty healthy adult volunteers. The overall survival was evaluated by Kaplan-Meier analysis with the log-rank test. Cell viability, proliferation and apoptosis were measured by CCK-8, EdU assay and Flow cytometry in U266 and MM.1R cells. Tumor growth was estimated by a xenograft assay. The apoptosis of tumor tissues was evaluated using TUNEL staining. The protein level in tissues was tested by immunohistochemistry. RESULTS: The HOXC6 expression was elevated in MM and high HOXC6 level was associated with the poor overall survival of MM. Besides, the HOXC6 expression was associated with hemoglobin level and ISS stage. Furthermore, silencing HOXC6 suppressed cell proliferation, induced cell apoptosis, and restrained the secretion of inflammatory factors (TNF-α, IL-6, and IL-8) in MM cells through inactivating the NF-κB pathway. Moreover, silencing HOXC6 suppressed the tumor growth of MM, the inflammatory factors levels, and the activation of NF-κB pathway but enhanced apoptosis in vivo. CONCLUSION: HOXC6 was elevated in MM and associated with poor survival. Knockdown of HOXC6 suppressed proliferation, inflammation and tumorigenicity of MM cells via inactivating the NF-κB pathway. HOXC6 may be a meaningful target for MM therapy.
Multiple Myeloma , NF-kappa B , Adult , Humans , NF-kappa B/genetics , NF-kappa B/metabolism , Multiple Myeloma/genetics , Multiple Myeloma/metabolism , Cell Proliferation/genetics , Prognosis , Inflammation , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism
During ageing, adult stem cells' regenerative properties decline, as they undergo replicative senescence and lose both their proliferative and differentiation capacities. In contrast, embryonic and foetal progenitors typically possess heightened proliferative capacities and manifest a more robust regenerative response upon injury and transplantation, despite undergoing many rounds of mitosis. How embryonic and foetal progenitors delay senescence and maintain their proliferative and differentiation capacities after numerous rounds of mitosis, remains unknown. It is also unclear if defined embryonic factors can rejuvenate adult progenitors to confer extended proliferative and differentiation capacities, without reprogramming their lineage-specific fates or inducing oncogenic transformation. Here, we report that a minimal combination of LIN28A, TERT, and sh-p53 (LTS), all of which are tightly regulated and play important roles during embryonic development, can delay senescence in adult muscle progenitors. LTS muscle progenitors showed an extended proliferative capacity, maintained a normal karyotype, underwent myogenesis normally, and did not manifest tumorigenesis nor aberrations in lineage differentiation, even in late passages. LTS treatment promoted self-renewal and rescued the pro-senescence phenotype of aged cachexia patients' muscle progenitors, and promoted their engraftment for skeletal muscle regeneration in vivo. When we examined the mechanistic basis for LIN28A's role in the LTS minimum combo, let-7 microRNA suppression could not fully explain how LIN28A promoted muscle progenitor self-renewal. Instead, LIN28A was promoting the translation of oxidative phosphorylation mRNAs in adult muscle progenitors to optimize mitochondrial reactive oxygen species (mtROS) and mitohormetic signalling. Optimized mtROS induced a variety of mitohormetic stress responses, including the hypoxic response for metabolic damage, the unfolded protein response for protein damage, and the p53 response for DNA damage. Perturbation of mtROS levels specifically abrogated the LIN28A-driven hypoxic response in Hypoxia Inducible Factor-1α (HIF1α) and glycolysis, and thus LTS progenitor self-renewal, without affecting normal or TS progenitors. Our findings connect embryonically regulated factors to mitohormesis and progenitor rejuvenation, with implications for ageing-related muscle degeneration.
Adult Stem Cells , Rejuvenation , Tumor Suppressor Protein p53/metabolism , Cell Differentiation , Adult Stem Cells/metabolism
Pesticide residues in grapes could be transferred to fermentation system during the wine-making process, which may interfere the normal proliferation of Saccharomyces cerevisiae and subsequently affect the safety and quality of wine products. However, the interaction between pesticides and Saccharomyces cerevisiae is still poorly understood. Herein, the fate, distribution and interaction effect with Saccharomyces cerevisiae of five commonly-used pesticides during the wine-making process were evaluated. The five pesticides exerted varied inhibition on the proliferation of Saccharomyces cerevisiae, and the order of inhibition intensity was difenoconazole > tebuconazole > pyraclostrobin > azoxystrobin > thiamethoxam. Compared with the other three pesticides, triazole fungicides difenoconazole and tebuconazole showed stronger inhibition and played a major role in binary exposure. The mode of action, lipophilicity and exposure concentration were important factors in the inhibition of pesticides. Saccharomyces cerevisiae had no obvious impacts on the degradation of target pesticides in the simulated fermentation experiment. However, the levels of target pesticides and their metabolite were significantly reduced during the wine-making process, with the processing factors ranged from 0.030 to 0.236 (or 0.032 to 0.257) during spontaneous (or inoculated) wine-making process. As a result, these pesticides were significantly enriched in the pomace and lees, and showed a positive correlation (R2 ≥ 0.536, n = 12, P < 0.05) between the hydrophobicity of pesticides and distribution coefficients in the solid-liquid distribution system. The findings provide important information for rational selection of pesticides on wine grapes and facilitate more accurate risk assessments of pesticides for grape processing products.
Fungicides, Industrial , Pesticide Residues , Pesticides , Vitis , Wine , Wine/analysis , Pesticides/toxicity , Pesticides/metabolism , Saccharomyces cerevisiae/metabolism , Pesticide Residues/analysis , Fungicides, Industrial/toxicity , Fungicides, Industrial/metabolism , Fermentation
Exploring transmission risk of different routes has major implications for epidemic control. However, disciplinary boundaries have impeded the dissemination of epidemic information, have caused public panic about "air transmission," "air-conditioning transmission," and "environment-to-human transmission," and have triggered "hygiene theater." Animal experiments provide experimental evidence for virus transmission, but more attention is paid to whether transmission is driven by droplets or aerosols and using the dichotomy to describe most transmission events. Here, according to characteristics of experiment setups, combined with patterns of human social interactions, we reviewed and grouped animal transmission experiments into four categories-close contact, short-range, fomite, and aerosol exposure experiments-and provided enlightenment, with experimental evidence, on the transmission risk of severe acute respiratory syndrome coronavirus (SARS-COV-2) in humans via different routes. When referring to "air transmission," context should be showed in elaboration results, rather than whether close contact, short or long range is uniformly described as "air transmission." Close contact and short range are the major routes. When face-to-face, unprotected, horizontally directional airflow does promote transmission, due to virus decay and dilution in air, the probability of "air conditioning transmission" is low; the risk of "environment-to-human transmission" highly relies on surface contamination and human behavior based on indirect path of "fomite-hand-mucosa or conjunctiva" and virus decay on surfaces. Thus, when discussing the transmission risk of SARS-CoV-2, we should comprehensively consider the biological basis of virus transmission, environmental conditions, and virus decay. Otherwise, risk of certain transmission routes, such as long-range and fomite transmission, will be overrated, causing public excessive panic, triggering ineffective actions, and wasting epidemic prevention resources.
Ceramics play an important role in human daily life and production practice. Pottery sculpture technique is the core of ceramic making. However, the production process of traditional ceramics is accompanied by high pollution, which has a great impact on human health and the ecological environment. Rapid development of industrialization has exacerbated this consequence. As the "Pottery Capital of Southern China", Foshan has been involved in environmental crises while relying on the ceramic industry to develop. Since the 21st century, Foshan has gradually successfully driven to upgrade the city from industrial to culture-led by carrying out positive innovations in Shiwan pottery sculpture technique. Therefore, based on the theoretical perspective of cultural ecology, this paper selects Shiwan pottery sculpture technique as the object, uses Python (Octopus Collector) to obtain data, and applies grounded theory to generate the ecological evolution model. This study discussed how the Shiwan pottery sculpture technique promotes the harmonious coexistence of human beings, industries, and cities in the new cultural ecological environment of the 21st century by exploring and clarifying the interaction and function of different elements in different stages of evolution. Finally, this study not only makes up for the current lack of research on Shiwan's cultural ecology, but also provides meaningful reference for environmental reform in other industrialized cities.
Ecology , Sculpture , Humans , China , Sculpture/history , Ceramics , Cities
The Turing pattern is an important dynamic behavior characteristic of activator-inhibitor systems. Differentiating from traditional assumption of activator-inhibitor interactions in a spatially continuous domain, a Turing pattern in networked reaction-diffusion systems has received much attention during the past few decades. In spite of its great progress, it still fails to evaluate the precise influences of network topology on pattern formation. To this end, we try to promote the research on this important and interesting issue from the point of view of average degree-a critical topological feature of networks. We first qualitatively analyze the influence of average degree on pattern formation. Then, a quantitative relationship between pattern formation and average degree, the exponential decay of pattern formation, is proposed via nonlinear regression. The finding holds true for several activator-inhibitor systems including biology model, ecology model, and chemistry model. The significance of this study lies that the exponential decay not only quantitatively depicts the influence of average degree on pattern formation, but also provides the possibility for predicting and controlling pattern formation.
Models, Biological , Models, Theoretical , Diffusion
In this article, we establish the local and global exponential convergence of a primal-dual dynamics (PDD) for solving equality-constrained optimization problems without strong convexity and full row rank assumption on the equality constraint matrix. Under the metric subregularity of Karush-Kuhn-Tucker (KKT) mapping, we prove the local exponential convergence of the dynamics. Moreover, we establish the global exponential convergence of the dynamics in an invariant subspace under a technically designed condition which is weaker than strong convexity. As an application, the obtained theoretical results are used to show the exponential convergence of several existing state-of-the-art primal-dual algorithms for solving distributed optimization without strong convexity. Finally, we provide some experiments to demonstrate the effectiveness of our results.
Competition and abiotic stress such as waterlogging (WL) represent main factors limiting plant growth and determining plant resistance and distribution patterns in wetland ecosystems. One of the basic steps for wetland restoration is to plant trees to ensure a quicker recovery and prevent erosion. Plant survival and adaptation are considered criteria of principal priority for the screening of plant species for wetland ecosystem restoration. WL influences plant species in wetlands via the deterioration of the plant root environment which leads to oxygen deficiency that affects plant growth, photosynthesis, respiration, and other metabolic processes. A suitable plant species was determined according to tolerance to WL during wetland vegetation recovery activities. Thus, two tree species (Cleistocalyx operculatus and Syzygium jambos) that showed a certain waterlogging tolerance were chosen to study their behaviors in different planting model and environmental conditions. Given that interspecific relationship should be considered during plant community construction, the eco-physiological adaptable mechanisms between these woody plants under well-watered condition and waterlogging stress were explored. Results showed that both species were waterlogging-tolerant species due to their ability to adapt to submergence conditions for 120 days. Moreover, C. operculatus possessed stronger tolerance to waterlogging stress because of a significant adventitious roots biomass accumulation. A competitive relationship was found between C. operculatus and S. jambos under well-watered condition, and C. operculatus showed better growth performance benefited from morphological responses (plant height, number of blade and leaf area) and endogenous hormone variations. In comparison, S. jambos suffered some negative effects when the well-watered mixed planting was used. However, the competitive relationship under well-watered condition was transformed into mutualistic relationship under waterlogging stress. The mixed planting under waterlogging condition significantly improved the tolerance of C. operculatus and S. jambos to waterlogging stress, compared with the monoculture., Especially, S. jambos showed improvements in root length, root surface area, and redox equilibration between lower levels of relative conductivity, malondialdehyde, and O 2 â - and had increased levels of non-enzymatic antioxidant components, such as reduced glutathione and soluble proteins. The interspecific relationship between C. operculatus and S. jambos was altered by waterlogging stress, and both showed improved tolerance to waterlogging stress. This study can provide a glimmer of light on suitable plant species selection and plant community construction during the revegetation activities in wetland zones. C. operculatus and S. jambos represent potential candidates in wetland restoration in a mixed planting model.
BACKGROUND: Pesticide contamination in oil crops and processed products is an important food safety concern. The study was aimed to investigate the pesticide residue changes in press processing of peanut oil and frying of chips. RESULTS: Five pesticides - chlorpyrifos, deltamethrin, methoxyfenozide, azoxystrobin and propargite - which are often applied during growth period in peanut plants, were selected to investigate their residue changes in cold press processing of peanut oil and frying of potato chips. Results showed that the residues of the five pesticides were decreased by 3.1-42.6% during air-drying before oil pressing. The residues of chlorpyrifos, deltamethrin, methoxyfenozide and propargite in peanut oil were 2.05-3.63 times higher than that in peanut meal after cold pressing of the oil, except for azoxystrobin having a slightly lower residue in peanut oil, with 0.92 times that in peanut meal. The processing factors of the five pesticides in peanut oil ranged from 1.17 to 2.73 and were highly related to the log Kow of the pesticides. The higher the log Kow , the more easily was the pesticide partitioned in the peanut oil. Besides, as frying time increase during preparation of chips, the concentration of pesticides in peanut oil decreased gradually by 6.7-22.1% compared to the first frying. In addition, 0.47-11.06% of the pesticides were transferred to the chips through frying with contaminated oil. CONCLUSION: This is first report showing that pesticides can transfer from contaminated oil to chips. There exists a potential dietary health risk by using pesticide-contaminated oil for frying chips. This work could provide basic data for accurate dietary risk assessment of pesticide residues in peanut oil and its frying products. © 2021 Society of Chemical Industry.
Chlorpyrifos , Pesticide Residues , Pesticides , Arachis , Cooking , Peanut Oil , Pesticide Residues/analysis , Pesticides/analysis
Cleistocalyx operculatus and Syzygium cumini possess a certain waterlogging tolerance. However, the comparable and adaptable strategies to waterlogging stress between these two species on the basis of waterlogging adventitious root (AR) regulation were still unclear. In this study, the plant performance in response to AR regulation based on AR removal (AR-R) and exogenous hormone application was investigated in terms of plant morphology, physiology, photosynthesis and AR traits. Results showed that C. operculatus possesses stronger waterlogging tolerance than S. cumini based on waterlogging tolerance coefficient, which is mainly due to the higher root biomass, root porosity and length, and activity of ARs, and shorter emergence time of ARs in C. operculatus than in S. cumini. The AR-R treatment increased activity and porosity of primary root, and induced a large amount of up-vertical ARs from the primary root systems in C. operculatus, while similar adaptive morphological changes in roots did not occur in AR-R-treated S. cumini. Exogenous abscisic acid (ABA) application had better effects on alleviating waterlogging damages than exogenous auxin (IAA) in balancing endogenous hormones (ABA and zeatin riboside), promoting AR development (porosity and activity, and the ratio of cortex area to stele area), improving the photosynthesis process and the antioxidant system (soluble protein, free proline and peroxidase). Moreover, under waterlogging conditions, exogenous ABA application induced greater increases in net photosynthesis rate, stomatal conductance, chlorophyll b and carotenoid in S. cumini than in C. operculatus, which suggested that S. cumini responded more positively and efficiently to exogenous ABA application than C. operculatus under waterlogging conditions. Thus, the findings provided new insights into the waterlogging adaptable strategies in waterlogging tolerant woody species on the basis of ARs and could provide scientific guidance for the application of these two species during revegetation activities in wetlands. Cleistocalyx operculatus could alternatively form a majority of up-vertical adventitious roots (ARs) from the primary roots after removing the normal ARs, but Syzygium cumini could not.Cleistocalyx operculatus possessed positive strategies to waterlogging stress, while S. cumini used traditional passive strategies.Exogenous abscisic acid (ABA) application had better effects on alleviating waterlogging damages in both species than exogenous auxin application.Syzygium cumini could more positively and efficiently respond to exogenous ABA application than C. operculatus.Waterlogging tolerance coefficient was significantly controlled by the chlorophyll contents and AR factors in C. operculatus and the AR factors and O2- in S. cumini.The best development of the AR number (ARN) and AR length (ARL) in exogenous ABA-treated C. operculatus may be closely related with positive zeatin riboside accumulation.The development of ARN and ARL was more important to waterlogging tolerance than that of AR porosity under waterlogging conditions.
Abscisic Acid , Plant Growth Regulators , Abscisic Acid/metabolism , Indoleacetic Acids/metabolism , Photosynthesis , Plant Roots
A novel deep eutectic solvent-based extraction and sulfur-doped carbon quantum dots (S-CQDs) serving as fluorescence probes to detect pirimicarb in cereals were established. The deep eutectic solvent was synthesized using choline chloride and butanediol, achieving direct and efficient extraction of pirimicarb residue in the cereals. The fluorescence quenching of S-CQDs was caused by the electrostatic interaction between the negatively charged S-CQDs and positively charged thiocholine, which was the hydrolysate of acetylthiocholine. The fluorescence of S-CQDs was enhanced as the activity of acetylcholinesterase (AChE) was inhibited by pirimicarb, achieving the detection of pirimicarb in the cereal samples. The limit of detection (LOD) was 0.006 µg mL-1. The recovery ranged from 96.6% to 108.2%. This extraction and detection method of pirimicarb based on an environmentally friendly DES and S-CQD fluorescent sensor maintains good stability and convenience, offering a promising strategy for extracting and testing harmful substances in food samples.
Edible Grain , Quantum Dots , Acetylcholinesterase , Carbamates , Pyrimidines , Solvents
