OBJECTIVE: To study the treatmaient of non-small cell lung cancer, we established the HU-Prim allograft transplantation tumor model. METHODS: The fresh tumor samples were transplanted in the right scapular subcutaneous layer of the severe combined immunodeficient Non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. The pathological features of the tumors were observed. Nonnecrotic tissue was inoculated subcutaneously into the right axillary. When the tumor in burdened rat grew approximately 100 mm3, according to the tumor size all the animals were divided into the following four groups, eight rats in each group:solvent control group, gefitinib group (100 mg/kg), erlotinib group (50 mg/kg), afatinib group (20 mg/kg). Aniamals were treated with drugs by intragastric (i.g.) administrated, once daily, for consecutively 14 days. Measure the tumor size 2-3 times every week. RESULTS: HuPrime1-NSCLC mutant sensitive xenograft model research data showed that reversible tyrosine kinase inhibitors gefitinib, erlotinib and irreversible tyrosine kinase inhibitor afatinib could effectively inhibit tumor growth in EGFR positive NSCLC allografts model. The pharmacodynamic activity of irreversible inhibitor was better than that of the reversible inhibitor. Specimens from clinical anthropogenic tumor retain characteristics of the human primary malignancy, histopathology, biological characteristics, and tumor markers, etc., which can more accurately reflect the characteristics of the tumor and the impact of interventions. CONCLUSIONS: The model is not only a good antitumor drug experimental platform, but also a new evaluation tool of individualized medication.
Antineoplastic Agents/pharmacology , Carcinoma, Non-Small-Cell Lung/drug therapy , Lung Neoplasms/drug therapy , Protein Kinase Inhibitors/pharmacology , Afatinib , Animals , Cell Line, Tumor , Erlotinib Hydrochloride/pharmacology , Gefitinib , Humans , Mice , Mice, Inbred NOD , Mice, SCID , Quinazolines/pharmacology , Xenograft Model Antitumor Assays
PURPOSE: We evaluated the efficiency of early endoscopic realignment as primary therapy for bulbar urethral disruption after straddle injury. MATERIALS AND METHODS: From 1990 to 1999 we treated 16 men who had bulbar urethral disruption with endoscopic realignment. Followup included uroflowmetry and urethroscopy at 39 to 85 months. RESULTS: All 16 cases were successfully treated at a single session without intraoperative or postoperative complications. Only 2 patients required intermittent self-dilation once weekly and all were potent during followup. CONCLUSIONS: The results of this minimally invasive procedure are comparable to those of open surgery. It may be performed on an outpatient basis using only local anesthesia. Our results imply that this cost-effective therapy should be done as the initial step in most patients with bulbar urethral disruption.
Urethra/injuries , Urethra/surgery , Urologic Surgical Procedures , Adolescent , Adult , Endoscopy , Female , Humans , Male , Minimally Invasive Surgical Procedures , Treatment Outcome , Urologic Surgical Procedures/methods
(E,Z)-3,13-OctadecadienyI acetate (1a) and (E,Z)-3,13-octadecadien-1-ol(2a) were identified from the sex pheromone gland of the virgin female mulberry clearwing mothParadoxecia pieli L., by GC analysis, EAG, SCR survey, and field bioassay. One female equivalent contained 250 ng of1a and 30 ng of2a. In the field tests, 100µg of synthetic1a was attractive to male moths of the species.
(S)-(+)-1-Methylbutyl (E)-2-methyl-2-pentenoate,1, and (S)-(+)-1-methylbutyl (E)-2,4-dimethyl-2-pentenoate2, the aggregation pheromone for lesser grain borerRhyzopertha dominica (F). were synthesized from crotylaldehyde in an overall yield of 30%. The chiral intermediate6 was prepared in 90% enantiomer excess, employing the Sharpless asymmetric epoxidation.
The thoracic gland of the ant-lionEuroleon nostras was found to contain nerol oxide (1a) and (Z)-6-undecen-2-ol (nostrenol,3) while the speciesGrocus bore contained 10-homonerol oxide (1b) and nostrenol (3). Nerol (2a) and 10-homonerol (2b) were found in a third species,Myrmeleon formicarius. 10-Homonerol, racemic 10-homonerol oxide, and racemic as well as (R)- and (S)-nostrenol were synthesized. The nerol oxide ofE. nostras and the 10-homonerol oxide ofG. bore were found to be racemic, while both species contained optically pure (R)-nostrenol (28).
(E,Z)-3,13-Octadecadien-1-ol (la) was identified as the sex pheromone from the poplar twig clearwing moth,Paranthrene tabaniformis, females by gas chromatography-mass spectrometry analysis, synthesis, and laboratory bioassays. In the field tests, a trap baited with 200 µg synthetic la caught more male moths than two live female moths.
