RESUMEN
The experiments on dogs subjected to the outward overheating allow concluding that the quantities of deaminated serotonin in mitochondrial fractions of the brain stem, heart muscles and liver increase twice as compared with the normal levels. Particularly, in the liver the value of Km of this process is equal to 1.6 x 10(-3) M, while without the overheating it is equal to 3.6 x 10(-3) M. Overheating makes the process of tryptamine deamination in mitochondrial fractions of the heart and liver more intensive. However, overheating causes a higher increase of the monoaminooxidase activity with respect to serotonin than with respect to other substrates.
Asunto(s)
Bencilaminas/metabolismo , Hipertermia Inducida , Mitocondrias/enzimología , Monoaminooxidasa/metabolismo , Serotonina/metabolismo , Triptaminas/metabolismo , Animales , Tronco Encefálico/enzimología , Desaminación , Perros , Femenino , Cinética , Masculino , Mitocondrias Cardíacas/enzimología , Mitocondrias Hepáticas/enzimología , Oxidación-Reducción , Especificidad por SustratoRESUMEN
The conjugated derivatives--1.4-dinitrobutene-2, 1,4-dinitro-2-methylbutene-2, 1,4-dinitro-2,3-diphenylbutene-2 sodium salts, as well as dinitromethane sodium salt and beta-nitrostyrol, the inhibitors of oxidative deamination of serotonin, tyramine, tryptamine and benzylamine in bovine liver mitochondria, were studied. All the derivatives under study were found to be active inhibitors of monoamine oxidase catalyzing the oxidative deamination of serotonin, tyramine and tryptamine. In a far lesser degree these preparations inhibited the deamination of benzylamine, a specific substrate for monoamine oxidase B. All the dinitrocompound dianions, with the exception of 1,4-dinitro-2,3-diphenylbutene-2 disodium salt, a non-competitive inhibitor of oxidative deamination of the four substrates under study, cause competitive reversible inhibition of monoamine oxidase.
Asunto(s)
Aminas/metabolismo , Bencilaminas/metabolismo , Mitocondrias Hepáticas/metabolismo , Serotonina/metabolismo , Triptaminas/metabolismo , Tiramina/metabolismo , Alquenos/farmacología , Animales , Bovinos , Desaminación , Técnicas In Vitro , Mitocondrias Hepáticas/efectos de los fármacos , Monoaminooxidasa/metabolismo , Inhibidores de la Monoaminooxidasa/farmacología , Nitrocompuestos/farmacología , Oxidación-Reducción , Especificidad por SustratoRESUMEN
The effect of 5-oxytryptophan, injected singly and in combination with the serotonin antagonists (novocain, atropine and bigumal) on the ascorbic acid level in the adrenal cortex and the eosinophilic content in the peripheral blood of white rats was studied. It was ascertained that 5-oxytryptophan reduced significantly the amount of ascorbic acid in the adrenal cortex and produced eosinopenia. Novocain, atropine and bigumal did not exert a similar effect. A combined injection of 5-oxytryptophan together with the mentioned antagonists of serotonin prevented eosinopenia evoked by 5-oxytryptophan. At the same time, the tested antagonists of serotonin did not change the effect of 5-oxytryptophan on the level of ascorbic acid in the adrenal cortex.