Rapid Method of Wastewater Classification by Electronic Nose for Performance Evaluation of Bioreactors with Activated Sludge.

Currently, e-noses are used for measuring odorous compounds at wastewater treatment plants. These devices mimic the mammalian olfactory sense, comprising an array of multiple non-specific gas sensors. An array of sensors creates a unique set of signals called a "gas fingerprint", which enables it to differentiate between the analyzed samples of gas mixtures. However, appropriate advanced analyses of multidimensional data need to be conducted for this purpose. The failures of the wastewater treatment process are directly connected to the odor nuisance of bioreactors and are reflected in the level of pollution indicators. Thus, it can be assumed that using the appropriately selected methods of data analysis from a gas sensors array, it will be possible to distinguish and classify the operating states of bioreactors (i.e., phases of normal operation), as well as the occurrence of malfunction. This work focuses on developing a complete protocol for analyzing and interpreting multidimensional data from a gas sensor array measuring the properties of the air headspace in a bioreactor. These methods include dimensionality reduction and visualization in two-dimensional space using the principal component analysis (PCA) method, application of data clustering using an unsupervised method by Density-Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm, and at the last stage, application of extra trees as a supervised machine learning method to achieve the best possible accuracy and precision in data classification.

A method for early detection and identification of fungal contamination of building materials using e-nose.

The aim of the study was to develop a method for early detection and identification of fungal contamination of building materials using an electronic nose. Therefore, the laboratory experiments based on the analysis of the air in the vicinity of fungal isolates potentially found in the building materials were performed. The results revealed that the employed gas sensors array consisting of MOS-type sensors enables the detection of the differences among the examined samples of fungi and distinguishing between the non-contaminated and contaminated samples, shortly after fungal contamination occurs. Electronic nose readouts were analysed using Principal Component Analysis and the results were verified with standard chromatographic analysis by means of SPME-GC/MS method, which proved that gas sensors array can be applied for early detection of fungal contamination.

Evaluation of Hydrocarbon Soil Pollution Using E-Nose.

The possibility of detecting low levels of soil pollution by petroleum fuel using an electronic nose (e-nose) was studied. An attempt to distinguish between pollution caused by petrol and diesel oil, and its relation to the time elapsed since the pollution event was simultaneously performed. Ten arable soils, belonging to various soil groups from the World Reference Base (WRB), were investigated. The measurements were performed on soils that were moistened to field capacity, polluted separately with both hydrocarbons, and then allowed to dry slowly over a period of 180 days. The volatile fingerprints differed throughout the course of the experiment, and, by its end, they were similar to those of the unpolluted soils. Principal component analysis (PCA) and artificial neural network (ANN) analysis showed that the e-nose results could be used to detect soil contamination and distinguish between pollutants and contamination levels.

Evaluating Soil Moisture Status Using an e-Nose.

The possibility of distinguishing different soil moisture levels by electronic nose (e-nose) was studied. Ten arable soils of various types were investigated. The measurements were performed for air-dry (AD) soils stored for one year, then moistened to field water capacity and finally dried within a period of 180 days. The volatile fingerprints changed during the course of drying. At the end of the drying cycle, the fingerprints were similar to those of the initial AD soils. Principal component analysis (PCA) and artificial neural network (ANN) analysis showed that e-nose results can be used to distinguish soil moisture. It was also shown that different soils can give different e-nose signals at the same moistures.

Application of gas sensor arrays in assessment of wastewater purification effects.

A gas sensor array consisting of eight metal oxide semiconductor (MOS) type gas sensors was evaluated for its ability for assessment of the selected wastewater parameters. Municipal wastewater was collected in a wastewater treatment plant (WWTP) in a primary sedimentation tank and was treated in a laboratory-scale sequential batch reactor (SBR). A comparison of the gas sensor array (electronic nose) response to the standard physical-chemical parameters of treated wastewater was performed. To analyze the measurement results, artificial neural networks were used. E-nose-gas sensors array and artificial neural networks proved to be a suitable method for the monitoring of treated wastewater quality. Neural networks used for data validation showed high correlation between the electronic nose readouts and: (I) chemical oxygen demand (COD) (r = 0.988); (II) total suspended solids (TSS) (r = 0.938); (III) turbidity (r = 0.940); (IV) pH (r = 0.554); (V) nitrogen compounds: N-NO3 (r = 0.958), N-NO2 (r = 0.869) and N-NH3 (r = 0.978); (VI) and volatile organic compounds (VOC) (r = 0.987). Good correlation of the abovementioned parameters are observed under stable treatment conditions in a laboratory batch reactor.