Synergistic effect of biochar with gypsum, lime, and farm manure on the growth and tolerance in rice plants under different salt-affected soils.

Soil salinization and sodication harm soil fertility and crop production, especially in dry regions. To combat this, using biochar combined with gypsum, lime, and farm manure is a promising solution for improving salt-affected soils. In a pot experiment, cotton stick biochar (BC) was applied at a rate of 20 t/ha in combination with gypsum (G), lime (L), and farm manure (F) at rates of 5 and 10 t/ha. These were denoted as BCG-5, BCL-5, BCF-5, BCG-10, BCL-10, and BCF-10. Three different types of soils with electrical conductivity (EC) to sodium adsorption ratio (SAR) ratios of 2.45:13.7, 9.45:22, and 11.56:40 were used for experimentation. The application of BCG-10 led to significant improvements in rice biomass, chlorophyll content, and overall growth. It was observed that applying BCG-10 to soils increased the membrane stability index by 75% in EC:SAR (2.45:13.7), 97% in EC:SAR (9.45:22), and 40% in EC:SAR (11.56:40) compared to respective control treatments. After BCG-10 was applied, the hydrogen peroxide in leaves dropped by 29%, 23%, and 21% in EC:SAR (2.45:13.7), EC:SAR (9.45:22), and EC:SAR (11.56:40) soils, relative to their controls, respectively. The application of BCG-10 resulted in glycine betaine increases of 60, 119, and 165% in EC: SAR (2.45:13.7), EC: SAR (9.45:22), and EC: SAR (11.56:40) soils. EC: SAR (2.45:13.7), EC: SAR (9.45:22), and EC: SAR (11.56:40) soils all had 70, 109, and 130% more ascorbic acid in BCG-10 applied treatment. The results of this experiment show that BCG-10 increased the growth and physiological traits of rice plants the most when they were exposed to different levels of salt stress. This was achieved by lowering hydrogen peroxide levels, making plant cells more stable, and increasing non-enzymatic activity.

Interactive Effects of Microbial Fertilizer and Soil Salinity on the Hydraulic Properties of Salt-Affected Soil.

Significant research has been conducted on the effects of fertilizers or agents on the sustainable development of agriculture in salinization areas. By contrast, limited consideration has been given to the interactive effects of microbial fertilizer (MF) and salinity on hydraulic properties in secondary salinization soil (SS) and coastal saline soil (CS). An incubation experiment was conducted to investigate the effects of saline soil types, salinity levels (non-saline, low-salinity, and high-salinity soils), and MF amounts (32.89 g kg-1 and 0 g kg-1) on soil hydraulic properties. Applied MF improved soil water holding capacity in each saline soil compared with that in CK, and SS was higher than CS. Applied MF increased saturated moisture, field capacity, capillary fracture moisture, the wilting coefficient, and the hygroscopic coefficient by 0.02-18.91% in SS, while it was increased by 11.62-181.88% in CS. It increased soil water supply capacity in SS (except for high-salinity soil) and CS by 0.02-14.53% and 0.04-2.34%, respectively, compared with that in CK. Soil available, readily available, and unavailable water were positively correlated with MF, while soil gravity and readily available and unavailable water were positively correlated with salinity in SS. Therefore, a potential fertilization program with MF should be developed to increase hydraulic properties or mitigate the adverse effects of salinity on plants in similar SS or CS areas.

Algal Bio-Stimulants Enhance Salt Tolerance in Common Bean: Dissecting Morphological, Physiological, and Genetic Mechanisms for Stress Adaptation.

Salinity adversely affects the plant's morphological characteristics, but the utilization of aqueous algal extracts (AE) ameliorates this negative impact. In this study, the application of AE derived from Chlorella vulgaris and Dunaliella salina strains effectively reversed the decline in biomass allocation and water relations, both in normal and salt-stressed conditions. The simultaneous application of both extracts in salt-affected soil notably enhanced key parameters, such as chlorophyll content (15%), carotene content (1%), photosynthesis (25%), stomatal conductance (7%), and transpiration rate (23%), surpassing those observed in the application of both AE in salt-affected as compared to salinity stress control. Moreover, the AE treatments effectively mitigated lipid peroxidation and electrolyte leakage induced by salinity stress. The application of AE led to an increase in GB (6%) and the total concentration of free amino acids (47%) by comparing with salt-affected control. Additionally, salinity stress resulted in an elevation of antioxidant enzyme activities, including superoxide dismutase, ascorbate peroxidase, catalase, and glutathione reductase. Notably, the AE treatments significantly boosted the activity of these antioxidant enzymes under salinity conditions. Furthermore, salinity reduced mineral contents, but the application of AE effectively counteracted this decline, leading to increased mineral levels. In conclusion, the application of aqueous algal extracts, specifically those obtained from Chlorella vulgaris and Dunaliella salina strains, demonstrated significant efficacy in alleviating salinity-induced stress in Phaseolus vulgaris plants.

Selenium-Containing Organic Fertilizer Application Affects Yield, Quality, and Distribution of Selenium in Wheat.

This study was designed to investigate the effect on wheat yield of applying organic fertilizers (OF) with five different selenium (Se) concentrations. The mineral nutrients, cadmium (Cd) content, and the distribution of Se in wheat plants were also measured. The results showed that wheat yields reached a maximum of 9979.78 kg ha-1 in Mengcheng (MC) County and 8868.97 kg ha-1 in Dingyuan (DY) County, Anhui Province, China when the application amount of selenium-containing organic fertilizer (SOF) was up to 600 kg ha-1. Among the six mineral nutrients measured, only the calcium (Ca) content of the grains significantly increased with an increase in the application amount of SOF in the two regions under study. Cd content showed antagonistic effects with the Se content of wheat grains, and when the SOF was applied at 1200 kg ha-1, the Cd content of the grains was significantly reduced by 30.1% in MC and 67.3% in DY, compared with under the Se0 treatment. After application of SOF, the Se content of different parts of the wheat plant ranked root > grain > spike-stalk > glume > leaf > stem. In summary, SOF application at a suitable concentration could increase wheat yields and significantly promote the Ca content of the grains. Meanwhile, the addition of Se effectively inhibited the level of toxic Cd in the wheat grains.

Effect of exogenous application of biogenic silicon sources on growth, yield, and ionic homeostasis of maize (Zea mays L.) crops cultivated in alkaline soil.

Salinity has emerged as a major threat to food security and safety around the globe. The crop production on agricultural lands is squeezing due to aridity, climate change and low quality of irrigation water. The present study investigated the effect of biogenic silicon (Si) sources including wheat straw biochar (BC-ws), cotton stick biochar (BC-cs), rice husk feedstock (RH-fs), and sugarcane bagasse (SB), on the growth of two consecutive maize (Zea mays L.) crops in alkaline calcareous soil. The application of SB increased the photosynthetic rate, transpiration rate, stomatal conductance, and internal CO2 concentration by 104, 100, 55, and 16% in maize 1 and 140, 136, 76, and 22% in maize 2 respectively. Maximum yield (g/pot) of cob, straw, and root were remained as 39.5, 110.7, and 23.6 while 39.4, 113.2, and 23.6 in maize 1 and 2 respectively with the application of SB. The concentration of phosphorus (P) in roots, shoots, and cobs was increased by 157, 173, and 78% for maize 1 while 96, 224, and 161% for maize 2 respectively over control by applying SB. The plant cationic ratios (Mg:Na, Ca:Na, K:Na) were maximum in the SB applied treatment in maize 1 and 2. The study concluded that the application of SB on the basis of soluble Si, as a biogenic source, remained the best in alleviating the salt stress and enhancing the growth of maize in rotation. The field trials will be more interesting to recommend the farmer scale.

Relationships between stable isotope natural abundances (δ13C and δ15N) and water use efficiency in rice under alternate wetting and drying irrigation in soils with high clay contents.

Natural abundance of the stable isotope (δ13C and δ15N) in plants is widely used to indicate water use efficiency (WUE). However, soil water and texture properties may affect this relationship, which remains largely elusive. Therefore, the purpose of this study was to evaluate δ13C as affected by different combinations of alternate wetting and drying irrigation (AWD) with varied soil clay contents in different organs and whole plant and assess the feasibility of using δ13C and δ15N as a physiological indicator of whole-plant water use efficiency (WUEwhole-plant). Three AWD regimes, I100 (30 mm flooded when soil reached 100% saturation), I90 (30 mm flooded when reached 90% saturation) and I70 (30 mm flooded when reached 70% saturation) and three soil clay contents, 40% (S40), 50% (S50), and 60% (S60), were included. Observed variations in WUEwhole-plant did not conform to theoretical expectations of the organs δ13C (δ13Corgans) of plant biomass based on pooled data from all treatments. However, a positive relationship between δ13Cleaf and WUEET (dry biomass/evapotranspiration) was observed under I90 regime, whereas there were no significant relationships between δ13Corgans and WUEET under I100 or I70 regimes. Under I100, weak relationships between δ13Corgans and WUEET could be explained by (i) variation in C allocation patterns under different clay content, and (ii) relatively higher rate of panicle water loss, which was independent of stomatal regulation and photosynthesis. Under I70, weak relationships between δ13Corgans and WUEET could be ascribed to (i) bigger cracks induced by water-limited irrigation regime and high clay content soil, and (ii) damage caused by severe drought. In addition, a negative relationship was observed between WUEwhole-plant and shoot δ15N (δ15Nshoot) across the three irrigation treatments, indicating that WUEwhole-plant is tightly associated with N metabolism and N isotope discrimination in rice. Therefore, δ13C should be used cautiously as an indicator of rice WUEwhole-plant at different AWD regimes with high clay content, whereas δ15N could be considered an effective indicator of WUEwhole-plant.

Improvement of Photosynthesis by Biochar and Vermicompost to Enhance Tomato (Solanum lycopersicum L.) Yield under Greenhouse Conditions.

Chlorophyll fluorescence is an important tool in the study of photosynthesis and its effect on the physiological indicators of crop growth is worth exploring. The trial was conducted to investigate the effect of biochar (CK, 0%; BA3, 3%; BA5, 5%; by mass of soil) and vermicompost (VA3, 3%; VA5, 5%) on photosynthesis, chlorophyll fluorescence, and tomato yield under greenhouse condition. Results revealed that photosynthetic parameters and chlorophyll fluorescence traits of BA3, VA3, BA5, and VA5 were significantly higher than those of CK, and the improvement of vermicompost was more effective than biochar at the same application rate. VA3 treatment had the highest net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), variable fluorescence (Fv), maximum fluorescence (Fm), PSII maximum photochemical efficiency (Fv/Fm), PSII potential photochemical activity (Fv/Fo), absorption flux per cross section (CS; ABC/CSm), trapped energy flux per CS (TRo/CSm), and electron transport flux per CS (ETo/CSm), which increased by 49%, 65%, 17%, 12%, 4%, 25%, 10%, 15%, and 30%, respectively, compared with CK. The study also found that BA and VA rates could effectively improve tomato yield and water use efficiency (WUE). The yield under BA3, VA3, BA5, and VA5 treatments was 21%, 33%, 23%, and 25% higher than that under CK, and the WUE increased from 31.2 kg·m-3 under CK to 41.4 kg·m-3 under VA3. Pearson correlation analysis indicated that the increment of photosynthesis showed a highly significant correlation with Fv/Fo, ABC/CSm, TRo/CSm, and ETo/CSm and enhanced the light energy absorbed, trapped, and transported per CS of plant leaves, thereby contributing to the increase in tomato yield. Therefore, for one-season tomato production, the application of 3% vermicompost was considered economical with regard to improving photosynthesis, enhancing WUE, and increasing tomato yield.

Natural 15N abundance as an indicator of nitrogen utilization efficiency in rice under alternate wetting and drying irrigation in soils with high clay contents.

The 15N natural abundance is an effective indicator of nitrogen dynamics in plants. The impact of different irrigation regimes as a function of varied soil clay contents on stable nitrogen isotope abundance (δ15N) in rice remains unknown. Therefore, the response of δ15N and nitrogen utilization efficiency (NUE) of rice to different combinations of alternate wetting and drying irrigation (AWD) and clay contents were investigated. The study included three AWD regimes, viz. I100, (100 % saturation, 30 mm flooded), I90 (90 % saturation, 30 mm flooded) and I70 (70 % saturation, 30 mm flooded), and three soil clay content treatments, viz. 40 % (S40), 50 % (S50), and 60 % (S60) clay content. Compared with I100, I90 and I70 with high clay content (S60) significantly increased the crack volumes and N leaching losses and reduced the total N accumulation and different forms of NUE of rice plants. The values of δ15N in above-ground organs and soil were greatly increased by I90 and I70 irrigation regimes compared to I100. An increasing trend of organs δ15N from root to shoot was found for all three irrigation regimes. Significant negative relationships were found between (i) N partial factor productivity (PFP) and grain 15N, (ii) PFP and leaf 15N, and (iii) N harvest index (NHI) and leaf 15N. These significant negative relationships might contribute to the increased N losses and changed N allocation under AWD with high clay contents. Hence, it is suggested that cracks should be taken into consideration in rice cultivation. Moreover, δ15N may serve as an effective indicator of NUE in rice grown under AWD irrigation with high clay contents as well as an indirect indicator for assessing the N loss in agro-ecosystems.

Dissecting the combined effects of cultivar, fertilization, and irrigation on rhizosphere bacterial communities and nitrogen productivity in rice.

Rice cultivars, fertilizer types, and irrigation modes can affect soil bacterial communities and thus influence nitrogen utilization by soil microorganisms and plants. However, the combined effects of these three factors on soil bacterial communities and nitrogen productivity in rice plants remain unknown. Here, we examined the response of rhizosphere bacteria and nitrogen productivity to different combinations of cultivar (japonica or indica), fertilization (organic plus chemical or chemical), and irrigation (controlled or shallow-frequent). The results demonstrated the interactive effects of cultivars with fertilizers and irrigation on rhizosphere bacterial communities, nitrogen accumulation, and grain yield. These significant interactive effects were related to differences in the response to soil environment (soil inorganic nitrogen concentration and moisture condition) between diverse rhizosphere bacteria recruited by indica and japonica. We found that rhizosphere bacterial communities recruited by indica were more active in soil fertilized with organic plus chemical nitrogen, while those recruited by japonica were suitable for living in soil fertilized with chemical nitrogen. Rhizosphere bacteria diversity positively correlated with soluble inorganic nitrogen in soil, suggesting that more diverse bacterial communities and greater contents of NH4+-N might favor nitrogen accumulation in rice plants under shallow-frequent irrigation. The combinations of cultivars, fertilizer types, and irrigation greatly affected rhizosphere bacterial communities, thus triggering a significant difference in soil inorganic nitrogen content, which could play an essential role in affecting nitrogen productivity.

A TEMPO-oxidized cellulose nanofibers/MOFs hydrogel with temperature and pH responsiveness for fertilizers slow-release.

In this work, a kind of MOF MIL-100(Fe)@CNFs hydrogel (MC) based on TEMPO-oxidized cellulose nanofibers (CNFs) for fertilizers slow-release was prepared by free-radical polymerization, where N-vinyl caprolactam (NVCL) and CNFs were involved to exhibit temperature and pH response, respectively. Particularly, porous MIL-100(Fe), a kind of metal organic frameworks (MOFs), was introduced to optimize the load and slow-release capabilities. The Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermogravimetric analysis were used to characterize. The swelling behaviors and water-retention capabilities of hydrogels were evaluated. Using urea as the model fertilizer, the slow-release mechanism was revealed. Wheat was used as the model crop to evaluate the practical growth status. Compared with MC-0% hydrogels, the MC-10% hydrogels exhibited a better swelling capacity (37 g/g), water-retention (22.78%) and slow-release performance (40.84%). It also exhibited sensitivities to temperature and pH for regulating urea release. Besides, the number of tillers and leaves of wheat fertilized with MC hydrogels significantly increased, as did the photosynthetic rate. In conclusion, the MC-0% hydrogels had a positive influence on crops growth, and promoted the possible utilization of hydrogels in slow-release fertilizers.

Thermo-/pH-responsive preservative delivery based on TEMPO cellulose nanofiber/cationic copolymer hydrogel film in fruit packaging.

Hydrogels have great potential in food packaging. However, stimuli-responsive preservative delivery-based hydrogels for emerging active packaging have not yet been explored. Herein, Unprecedented pH/temperature-responsive hydrogel films for emerging active climacteric fruit packaging were developed based on TEMPO-oxidized nanofibrillated cellulose (TOCNFs) from wheat straw with food-grade cationic-modified poly(N-isopropyl acrylamide-co-acrylamide) (CPNIPAM-AM). TOCNF incorporation into CPNIPAM-AM revealed desirable enhancement of characterization, antimicrobial properties, and pH/thermal-responsive behaviour. In-vitro delivery and release mechanism studies with natamycin revealed the fastest release rates in preferred low pH media, up to 32.1 times higher than that under neutral conditions via anomalous diffusion. Applying a thermal stimulus increased natamycin release rates, providing 1.5-21% gradual-additional pulses by Fickian diffusion. The final hydrogel film showed efficient decay control in response to stimuli of the climacteric fruit environment with safe, recyclable, and feasible application demonstrating the significant potential to be used as an alternative-sustainable material for stimuli-triggered preservative delivery in climacteric fruit packaging.

Zinc oxide nanoparticles: potential effects on soil properties, crop production, food processing, and food quality.

The use of zinc oxide nanoparticles (ZnO NPs) is expected to increase soil fertility, crop productivity, and food quality. However, the potential effects of ZnO NP utilization should be deeply understood. This review highlights the behavior of ZnO NPs in soil and their interactions with the soil components. The review discusses the potential effects of ZnO NPs on plants and their mechanisms of action on plants and how these mechanisms are related to their physicochemical properties. The impact of current applications of ZnO NPs in the food industry is also discussed. Based on the literature reviewed, soil properties play a vital role in dispersing, aggregation, stability, bioavailability, and transport of ZnO NPs and their release into the soil. The transfer of ZnO NPs into the soil can affect the soil components, and subsequently, the structure of plants. The toxic effects of ZnO NPs on plants and microbes are caused by various mechanisms, mainly through the generation of reactive oxygen species, lysosomal destabilization, DNA damage, and the reduction of oxidative stress through direct penetration/liberation of Zn2+ ions in plant/microbe cells. The integration of ZnO NPs in food processing improves the properties of the relative ZnO NP-based nano-sensing, active packing, and food/feed bioactive ingredients delivery systems, leading to better food quality and safety. The unregulated/unsafe discharge concentrations of ZnO NPs into the soil, edible plant tissues, and processed foods raise environmental/safety concerns and adverse effects. Therefore, the safety issues related to ZnO NP applications in the soil, plants, and food are also discussed.

Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhizal fungi and Bradyrhizobium under drought stress.

BACKGROUND: The present study aims to study the effects of biofertilizers potential of Arbuscular Mycorrhizal Fungi (AMF) and Bradyrhizobium japonicum (B. japonicum) strains on yield and growth of drought stressed soybean (Giza 111) plants at early pod stage (50 days from sowing, R3) and seed development stage (90 days from sowing, R5). RESULTS: Highest plant biomass, leaf chlorophyll content, nodulation, and grain yield were observed in the unstressed plants as compared with water stressed-plants at R3 and R5 stages. At soil rhizosphere level, AMF and B. japonicum treatments improved bacterial counts and the activities of the enzymes (dehydrogenase and phosphatase) under well-watered and drought stress conditions. Irrespective of the drought effects, AMF and B. japonicum treatments improved the growth and yield of soybean under both drought (restrained irrigation) and adequately-watered conditions as compared with untreated plants. The current study revealed that AMF and B. japonicum improved catalase (CAT) and peroxidase (POD) in the seeds, and a reverse trend was observed in case of malonaldehyde (MDA) and proline under drought stress. The relative expression of the CAT and POD genes was up-regulated by the application of biofertilizers treatments under drought stress condition. Interestingly a reverse trend was observed in the case of the relative expression of the genes involved in the proline metabolism such as P5CS, P5CR, PDH, and P5CDH under the same conditions. The present study suggests that biofertilizers diminished the inhibitory effect of drought stress on cell development and resulted in a shorter time for DNA accumulation and the cycle of cell division. There were notable changes in the activities of enzymes involved in the secondary metabolism and expression levels of GmSPS1, GmSuSy, and GmC-INV in the plants treated with biofertilizers and exposed to the drought stress at both R3 and R5 stages. These changes in the activities of secondary metabolism and their transcriptional levels caused by biofertilizers may contribute to increasing soybean tolerance to drought stress. CONCLUSIONS: The results of this study suggest that application of biofertilizers to soybean plants is a promising approach to alleviate drought stress effects on growth performance of soybean plants. The integrated application of biofertilizers may help to obtain improved resilience of the agro ecosystems to adverse impacts of climate change and help to improve soil fertility and plant growth under drought stress.

Seed priming and foliar application with jasmonic acid enhance salinity stress tolerance of soybean (Glycine max L.) seedlings.

BACKGROUND: Jasmonic acid (JA) is an important molecule that has a regulatory effect on many physiological processes in plant growth and development under abiotic stress. This study investigated the effect of 60 µmol L-1 of JA in seed priming (P) at 15 °C in darkness for 24 h, foliar application (F), and/or their combination effect (P + F) on two soybean cultivars - 'Nannong 99-6' (salt tolerant) and 'Lee 68' (salt sensitive) - under salinity stress (100 mmol L-1 sodium chloride (NaCl)). RESULTS: Salinity stress reduced seedling growth and biomass compared with that in the control condition. Priming and foliar application with JA and/or their combination significantly improved water potential, osmotic potential, water use efficiency, and relative water content of both cultivars under salinity stress. Similarly, seed priming with JA, foliar application of JA, and/or their combination significantly improved the following properties under salinity stress compared with the untreated seedlings: net photosynthetic rate by 68.03%, 59.85%, and 76.67% respectively; transpiration rate by 74.85%, 55.10%, and 80.26% respectively; stomatal conductance by 69.88%, 78.25%, and 26.24% respectively; intercellular carbon dioxide concentration by 61.64%, 40.06%, and 65.79% respectively; and total chlorophyll content by 47.41%, 41.02%, and 55.73% respectively. Soybean plants primed, sprayed with JA, or treated with their combination enhanced the chlorophyll fluorescence, which was damaged by salinity stress. JA treatments improved abscisic acid, gibberellic acid, and JA levels by 60.57%, 62.50% and 52.25% respectively under salt stress compared with those in the control condition. The transcriptional levels of the FeSOD, POD, CAT, and APX genes increased significantly in the NaCl-stressed seedlings irrespective of JA treatments. Moreover, JA treatment resulted in a reduction of sodium ion concentration and an increase of potassium ion concentrations in the leaf and root of both cultivars regardless of salinity stress. Monodehydroascorbate reductase, dehydroascorbate reductase, and proline contents decreased in the seedlings treated with JA under salinity stress, whereas the ascorbate content increased with JA treatment combined with NaCl stress. CONCLUSION: The application of 60 µmol L-1 JA improved plant growth by regulating the interaction between plant hormones and hydrogen peroxide, which may be involved in auxin signaling and stomatal closure under salt stress. These methods could efficiently protect early seedlings and alleviate salt stress damage and provide possibilities for use in improving soybean growth and inducing tolerance against excessive soil salinity. © 2020 Society of Chemical Industry.

Comparison of five Boosting-based models for estimating daily reference evapotranspiration with limited meteorological variables.

Accurate ET0 estimation is of great significance in effective agricultural water management and realizing future intelligent irrigation. This study compares the performance of five Boosting-based models, including Adaptive Boosting(ADA), Gradient Boosting Decision Tree(GBDT), Extreme Gradient Boosting(XGB), Light Gradient Boosting Decision Machine(LGB) and Gradient boosting with categorical features support(CAT), for estimating daily ET0 across 10 stations in the eastern monsoon zone of China. Six different input combinations and 10-fold cross validation method were considered for fully evaluating model accuracy and stability under the condition of limited meteorological variables input. Meanwhile, path analysis was used to analyze the effect of meteorological variables on daily ET0 and their contribution to the estimation results. The results indicated that CAT models could achieve the highest accuracy (with global average RMSE of 0.5667 mm d-1, MAE of 4199 mm d-1and Adj_R2 of 0.8514) and best stability regardless of input combination and stations. Among the inputted meteorological variables, solar radiation(Rs) offers the largest contribution (with average value of 0.7703) to the R2 value of the estimation results and its direct effect on ET0 increases (ranging 0.8654 to 0.9090) as the station's latitude goes down, while maximum temperature (Tmax) showes the contrary trend (ranging from 0.8598 to 0.5268). These results could help to optimize and simplify the variables contained in input combinations. The comparison between models based on the number of the day in a year (J) and extraterrestrial radiation (Ra) manifested that both J and Ra could improve the modeling accuracy and the improvement increased with the station's latitudes. However, models with J could achieve better accuracy than those with Ra. In conclusion, CAT models can be most recommended for estimating ET0 and input variable J can be promoted to improve model performance with limited meteorological variables in the eastern monsoon zone of China.

GABA-Alleviated Oxidative Injury Induced by Salinity, Osmotic Stress and their Combination by Regulating Cellular and Molecular Signals in Rice.

This study was conducted in order to determine the effect of priming with γ-aminobutyric acid (GABA) at 0.5 mM on rice (Oryza sativa L.) seed germination under osmotic stress (OS) induced by polyethylene glycol (30 g/L PEG 6000); and salinity stress (S, 150 mM NaCl) and their combination (OS+S). Priming with GABA significantly alleviated the detrimental effects of OS, S and OS+S on seed germination and seedling growth. The photosynthetic system and water relation parameters were improved by GABA under stress. Priming treatment significantly increased the GABA content, sugars, protein, starch and glutathione reductase. GABA priming significantly reduced Na+ concentrations, proline, free radical and malonaldehyde and also significantly increased K+ concentration under the stress condition. Additionally, the activities of antioxidant enzymes, phenolic metabolism-related enzymes, detoxification-related enzymes and their transcription levels were improved by GABA priming under stress. In the GABA primed-plants, salinity stress alone resulted in an obvious increase in the expression level of Calcineurin B-like Protein-interacting protein Kinases (CIPKs) genes such as OsCIPK01, OsCIPK03, OsCIPK08 and OsCIPK15, and osmotic stress alone resulted in obvious increase in the expression of OsCIPK02, OsCIPK07 and OsCIPK09; and OS+S resulted in a significant up-regulation of OsCIPK12 and OsCIPK17. The results showed that salinity, osmotic stresses and their combination induced changes in cell ultra-morphology and cell cycle progression resulting in prolonged cell cycle development duration and inhibitory effects on rice seedlings growth. Hence, our findings suggested that the high tolerance to OS+S is closely associated with the capability of GABA priming to control the reactive oxygen species (ROS) level by inducing antioxidant enzymes, secondary metabolism and their transcription level. This knowledge provides new evidence for better understanding molecular mechanisms of GABA-regulating salinity and osmotic-combined stress tolerance during rice seed germination and development.