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From a chemical infrastructure perspective, it is important to ensure that all ions constituting a target product, e.g., Zr and B ions for ZrB2, are fully linked with a cross-linking structure for synthesis via an organic precursor. In the present study, glycerol is used as a chelating ligand to prepare boron both partially and fully linked with the cross-linking structure of organic precursors by a sol-gel route. The results are far from expected, in that the more linked boron there is in the precursor, the purer the ZrB2 produced. In the case of a partially linked cross-linking structure, the carbothermic reduction reaction for ZrB2 is a multi-step process with an intermediary phase of ZrC, and then a high-purity prism-like ZrB2 powder with a larger size is obtained. A minimum of 0.26 wt% for the oxygen content of ZrB2 corresponds to a 0.67 molar ratio of glycerol to H3BO3. On the other hand, in the case with the boron fully linked, a single-phase of ZrB2 cannot be obtained, and instead a double-phase is obtained. Therefore, the amount of impurity is greater, even though the size is smaller. The carbothermic reduction reaction is direct, and has only one step.
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Mast cells can recognize foot-and-mouth disease virus-like particles (FMDV-VLPs) via mannose receptors (MRs) to produce differentially expressed cytokines. The regulatory role of chromatin accessibility in this process is unclear. Bone marrow-derived mast cells (BMMCs) were cultured, and an assay of transposase-accessible chromatin sequencing (ATAC-seq) was applied to demonstrate the regulation of chromatin accessibility in response to the BMMCs' recognition of FMDV-VLPs. A pathway enrichment analysis showed that peaks associated with the nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3 kinase-protein kinase B (PI3K-Akt), and other signaling pathways, especially the NF-κB pathway, were involved in the BMMCs' recognition of VLPs. Moreover, transcription factors including SP1, NRF1, AP1, GATA3, microphthalmia-associated transcription factor (MITF), and NF-κB-p65 may bind to the motifs with altered chromatin accessibility to regulate gene transcription. Furthermore, the expression of NF-κB, interleukin (IL)-9, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ in the BMMCs of the VLP group increased compared with that of the BMMCs in the control group, whereas the expression of IL-10 did not differ significantly between groups. After inhibiting the MRs, the expression of NF-κB, IL-9, TNF-α, and IFN-γ decreased significantly, whereas the expression of IL-10 increased. The expression of MAPK and IL-6 showed no significant change after MR inhibition. This study demonstrated that MRs expressed on BMMCs can affect the NF-κB pathway by changing chromatin accessibility to regulate the transcription of specific cytokines, ultimately leading to the differential expression of cytokines. These data provide a theoretical basis and new ideas for the development of a novel vaccine for FMD.
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Virus de la Fiebre Aftosa , FN-kappa B , Animales , FN-kappa B/metabolismo , Interleucina-10 , Virus de la Fiebre Aftosa/genética , Fosfatidilinositol 3-Quinasas/metabolismo , Secuenciación de Inmunoprecipitación de Cromatina , Citocinas/metabolismo , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Cromatina/genéticaRESUMEN
Surrogate models are commonly used as a substitute for the computation-intensive simulations in design optimization. However, building a high-accuracy surrogate model with limited samples remains a challenging task. In this paper, a novel adaptive-weight ensemble surrogate modeling method is proposed to address this challenge. Instead of using a single error metric, the proposed method takes into account the position of the prediction sample, the mixture error metric and the learning characteristics of the component surrogate models. The effectiveness of proposed ensemble models are tested on five highly nonlinear benchmark functions and a finite element model for the analysis of the frequency response of an automotive exhaust pipe. Comparative results demonstrate the effectiveness and promising potential of proposed method in achieving higher accuracy.
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Molecular simulations are currently receiving significant attention for their ability to offer a microscopic perspective that explains macroscopic phenomena. An essential aspect is the accurate characterization of molecular structural parameters and the development of realistic numerical models. This study investigates the surface morphology and elemental distribution of silicon nitride fibers through TEM and EDS, and SEM and EDS analyses. Utilizing a customized molecular dynamics approach, molecular models of amorphous and multi-interface silicon nitride fibers with complex structures were constructed. Tensile simulations were conducted to explore correlations between performance and molecular structural composition. The results demonstrate successful construction of molecular models with amorphous, amorphous-crystalline interface, and mixed crystalline structures. Mechanical property characterization reveal the following findings: (1) The nonuniform and irregular amorphous structure causes stress concentration and crack formation under applied stress. Increased density enhances material strength but leads to higher crack sensitivity. (2) Incorporating a crystalline reinforcement phase without interfacial crosslinking increases free volume and relative tensile strength, improving toughness and reducing crack susceptibility. (3) Crosslinked interfaces effectively enhance load transfer in transitional regions, strengthening the material's tensile strength, while increased density simultaneously reduces crack propagation.
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In practical application situations, a carbon fiber-reinforced polymer (CFRP) is often subjected to complex dynamic loadings. The effect of the strain rate on mechanical properties is very important for the CFRP design and product development. In this work, static and dynamic tensile properties of CFRP with different stacking sequences and ply orientations were investigated. The results showed that the tensile strengths of CFRP laminates were sensitive to the strain rate, while Young's modulus was independent of the strain rate. Moreover, the strain rate effect was related to the stacking sequences and ply orientations. The experimental results showed that the strain rate effects of the cross-ply laminates and quasi-isotropic-ply laminates were lower than that of the unidirectional-ply laminates. Finally, the failure modes of CFRP laminates were investigated. Failure morphology demonstrated that the differences in strain rate effects among cross-ply laminates, quasi-isotropic-ply laminates, and unidirectional-ply laminates were caused by the mismatch between the fiber and the matrix when the strain rate increased.
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Weld lines are a common defect generated in injection molding, which apparently affects the performance of final products, but the available reports on carbon fiber-reinforced thermoplastics are still rather few. In this study, the effects of injection temperature, injection pressure, and fiber content on the mechanical properties of weld lines were studied for carbon fiber-reinforced nylon (PA-CF) composites. The weld line coefficient was also calculated by comparing specimens with and without weld lines. The tensile and flexural properties of PA-CF composites significantly increased with the rise of fiber content for specimens without weld lines, while injection temperature and pressure demonstrated slight influences on mechanical properties. However, the existence of weld lines had negative influences on the mechanical properties of PA-CF composites due to poor fiber orientation in weld line regions. The weld line coefficient of PA-CF composites decreased as fiber content increased, indicating that the damage of weld lines to mechanical properties increased. The microstructure analysis showed that there were a large number of fibers distributed vertically to flow direction in weld lines regions, which could not play a reinforcing role. In addition, increasing injection temperature and pressure facilitated fiber orientation, which improved the mechanical properties of composites with low fiber content, while weakening composites with high fiber content instead. This article provides practical information for product design containing weld lines, which helps to optimize the forming process and formula design of PA-CF composites with weld lines.
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Foot-and-mouth disease (FMD) is one of the most economically and socially devastating diseases affecting animal agriculture worldwide. Foot-and-mouth disease virus (FMDV) virus-like particles (VLPs) have been widely studied as a candidate vaccine. Mast cells (MCs) are highly versatile innate immunity cells that perform various functions in regulating innate and adaptive immune responses. Recently, we found that MCs can recognize recombinant FMDV VP1-VP4 protein to produce various cytokines with differential expression, suggesting that this may be epigenetically regulated. In this study, we evaluated the effect of trichostatin A (TSA), a histone deacetylase inhibitor, on bone marrow-derived mast cells (BMMCs) recognition of FMDV-VLPs in vitro. BMMCs can recognize FMDV-VLPs via mannose receptors (MRs) and resulted in enhanced expression and secretion of tumour necrosis factor α (TNF-α) and interleukin (IL)-13. Nevertheless, BMMCs recognition of FMDV-VLPs to secrete IL-6 was irrelevant to MRs, and MRs may play a negative regulation for IL-10 secretion. Pre-treatment with TSA caused decreased expression of IL-6, TNF-α and IL-13, and increased expression of IL-10. Furthermore, the expression of nuclear factor-kappa B (NF-κB) was supressed in TSA treated BMMCs, suggesting histone acetylation may alter NF-κB expression to influence the TNF-α and IL-13 secretion. Pre-treatment with TSA had no influence on the expression of microphthalmia-associated transcription factor (MITF) and GATA-2. These data therefore suggest that altered histone acetylation regulates the immune responses induced by BMMCs recognition of FMDV-VLPs, providing an understanding and theory basis for the prevention and control of FMD based MCs.
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Virus de la Fiebre Aftosa , Fiebre Aftosa , Animales , Fiebre Aftosa/prevención & control , Histonas/metabolismo , Interleucina-10/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Interleucina-13/metabolismo , FN-kappa B/metabolismo , Acetilación , Interleucina-6/metabolismo , Proteínas RecombinantesRESUMEN
A challenging but critical question is that new foot-and-mouth disease (FMD) vaccines should be to induce B cell memory to provide antibodies for long-term protection. The maintenance of B cell memory is dependent on long-lived plasma cells (LLPCs) and memory B cells. We developed a chimeric FMDV virus-like particles (FMDV-VLPs), fusing VP1-VP4 into HBcAg. In our study, we investigated if or how long B cell memory was induced by FMDV-VLPs in mice. The data showed that FMDV-VLPs can induce memory humoral responses with a high level of total IgG1, IgG2a, IgA, and FMDV-specific IgG antibodies in serum. The persistence of antibody levels in serum could depend on LLPCs. The proportion of LLPCs in CD19+ cells in bone marrow exhibited a dynamic trend with two peaks at 28 days post-immunization (dpi) and 72 dpi, respectively. Additionally, the proportion of memory B cells in CD19+ cells in the spleen increased significantly both at 7 days post primary immunization and at 7 days post -boost immunization. Of note, LLPCs together with memory B cells contribute to the production of FMDV-specific IgG and IgG1. The changes of LLPCs and memory B cells may be related to TNF-α, IL-6 and, CXCL12. Taken together, FMDV-VLPs could induce B cells memory responses. A further understanding of the mechanisms that FMDV-VLPs how we can manipulate the induction and maintenance of memory B cells and LLPCs will promote vaccine design and likely address several challenges to develop FMDV new vaccines in the future.
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Virus de la Fiebre Aftosa , Fiebre Aftosa , Enfermedades de los Roedores , Vacunas de Partículas Similares a Virus , Vacunas Virales , Animales , Anticuerpos Antivirales , Fiebre Aftosa/prevención & control , Inmunoglobulina G , Ratones , Ratones Endogámicos BALB CRESUMEN
In this work, multi-scale finite element analyses based on three-dimensional (3D) hybrid macro/micro-scale computational models subjected to various loading conditions are carried out to examine the in-situ effect imposed by the neighboring plies on the failure initiation and propagation of cross-ply laminates. A detailed comparative study on crack suppression mechanisms due to the effect of embedded laminar thickness and adjacent ply orientation is presented. Furthermore, we compare the results of in-situ transverse failure strain and strength between the computational models and analytical predictions. Good agreements are generally observed, indicating the constructed computational models are highly accurate to quantify the in-situ effect. Subsequently, empirical formulas for calculating the in-situ strengths as a function of embedded ply thickness and different ply angle between embedded and adjacent plies are developed, during which several material parameters are obtained using a reverse fitting method. Finally, a new set of failure criteria for σ 22-τ 12, σ 22-τ 23, and σ 11-τ 12 accounting for the in-situ strengths are proposed to predict laminated composites failure under multi-axial stress states. This study demonstrates an effective and efficient computational technique towards the accurate prediction of the failure behaviors and strengths of cross-ply laminates by including the in-situ effects.
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The application of advanced ceramic materials is limited by their brittleness and complicated manufacturing methods. Three-dimensional (3D) printing has emerged as a new method for the fabrication of complex-shaped ceramics. Herein, a type of printable slurry composed of SiBCN preceramic polymers and high-volume fractions of Si3N4 whiskers (up to 60 wt% of polymer) was prepared, and subsequently printed via digital light processing (DLP) technology. We successfully manufactured complex-structured ceramic composites and achieved high bending strength (â¼180 MPa). The linear shrinkage and mass loss of the ceramic material were both significantly reduced after the introduction of whiskers. The properties and structure of the printed parts pyrolyzed at different temperatures were compared, and the relationship between the microstructure and mechanical properties discussed.
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Electrification of transportation offers clear national energy security benefits but unclear climate benefits. With the current heterogeneity of grid electricity mix in China, greenhouse gas (GHG) benefits of battery electric vehicles (BEVs) vary dramatically with location. Currently, compared to baseline conventional gasoline vehicles, BEVs in north and northeastern Chinese provinces have very modest (â¼10-20%) well-to-wheel (WTW) GHG benefits, whereas BEVs in southern provinces have substantial benefits (â¼50%). With the expected transition to a more renewable electricity mix documented here, regional effects will largely disappear and the benefits of BEVs will be substantial (â¼60-70% lower than current internal combustion engine vehicles (ICEVs) and â¼10-40% lower than 2030 advanced hybrid electric vehicles (HEVs)) across the whole of China by 2030. GHG emissions from BEVs in Chinese cities (Beijing, Shanghai, Chongqing, and Pearl River Delta) and United States cities and regions (New York; Washington, DC; Chicago; New England; Texas; and California) in 2015 and 2030 are evaluated and compared. BEVs in Chinese cities will still have substantially higher WTW GHG emissions than those in New York, New England, and California in 2030.
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Gases de Efecto Invernadero , Beijing , California , Chicago , China , Ciudades , Electricidad , Efecto Invernadero , Vehículos a Motor , New England , New York , Texas , Estados Unidos , Emisiones de VehículosRESUMEN
Car insurance is quickly becoming a big data industry, with usage-based insurance (UBI) poised to potentially change the business of insurance. Telematics data, which are transmitted from wireless devices in car, are widely used in UBI to obtain individual-level travel and driving characteristics. While most existing studies have introduced telematics data into car insurance pricing, the telematics-related characteristics are directly obtained from the raw data. In this study, we propose to quantify drivers' familiarity with their driving routes and develop models to quantify drivers' accident risks using the telematics data. In addition, we build a latent class model to study the heterogeneity in travel and driving styles based on the telematics data, which has not been investigated in literature. Our main results include: (1) the improvement to the model fit is statistically significant by adding telematics-related characteristics; (2) drivers' familiarity with their driving trips is critical to identify high risk drivers, and the relationship between drivers' familiarity and accident risks is non-linear; (3) the drivers can be classified into two classes, where the first class is the low risk class with 0.54% of its drivers reporting accidents, and the second class is the high risk class with 20.66% of its drivers reporting accidents; and (4) for the low risk class, drivers with high probability of reporting accidents can be identified by travel-behavior-related characteristics, while for the high risk class, they can be identified by driving-behavior-related characteristics. The driver's familiarity will affect the probability of reporting accidents for both classes.
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Accidentes de Tránsito , Conducción de Automóvil , Conducta , Seguro , Modelos Biológicos , Adulto , Beijing , Femenino , Humanos , Masculino , Persona de Mediana Edad , Reconocimiento en Psicología , Riesgo , Asunción de RiesgosRESUMEN
A hierarchically porous zirconia (ZrO2) monolith was successfully fabricated by using bacterial cellulose (BC) as a biotemplate and preceramic polymer as a zirconium resource, via freeze-drying and two-step calcination process. Images of scanning electron microscopy showed that the ZrO2 monolith well-replicated a three-dimensional reticulated structure of pristine BC and possessed good morphology stability till 1100 °C in air. Results of N2 adsorption/desorption and mercury porosimetry analysis revealed the hierarchically porous structure and large specific area (9.7 m2·g-1) of the ZrO2 monolith, respectively. Patterns of X-ray powder diffraction indicated that the monoclinic phase and tetragonal phase coexisted in the ZrO2 monolith with the former as the main phase. In addition, the ZrO2 monolith possessed low bulk density (0.13 g·cm-3) and good mechanical strength. These properties suggest that the as-prepared ZrO2 monolith has a great potential to serve as an ideal catalyst or catalyst support.
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The Beijing-Tianjin-Hebei (BTH) region is one of the most heavily polluted regions in China, with both high PM2.5 concentrations and a high population density. A quantitative source-receptor relationship can provide valuable insights that can inform effective emission control strategies. Both source apportionment (SA) and source sensitivity (SS) can provide such information from different perspectives. In this study, both methods are applied in northern China to identify the most significant emission categories and source regions for PM2.5 exposure in BTH in 2013. Despite their differences, both models show similar distribution patterns for population and simulated PM2.5 concentrations, resulting in overall high PM2.5 exposure values (approximately 110µg/m3) and particularly high exposure values during the winter (approximately 200µg/m3). Both methods show that local emissions play a dominant role (70%), with some contribution from surrounding provinces (e.g., Shandong) via regional transport. The two methods also agree on the priority of local emission controls: both identify industrial, residential, and agricultural emissions as the top three categories that should be controlled locally. In addition, the effect of controlling agricultural ammonia emissions is approximately doubled when the co-benefits of reducing nitrate are considered. The synthesis of SA and SS for addressing specific categories of emissions provides a quantitative basis for the development of emission control strategies and policies for controlling PM2.5 in China.
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China's oil imports and greenhouse gas (GHG) emissions have grown rapidly over the past decade. Addressing energy security and GHG emissions is a national priority. Replacing conventional vehicles with electric vehicles (EVs) offers a potential solution to both issues. While the reduction in petroleum use and hence the energy security benefits of switching to EVs are obvious, the GHG benefits are less obvious. We examine the current Chinese electric grid and its evolution and discuss the implications for EVs. China's electric grid will be dominated by coal for the next few decades. In 2015 in Beijing, Shanghai, and Guangzhou, EVs will need to use less than 14, 19, and 23 kWh/100 km, respectively, to match the 183 gCO2/km WTW emissions for energy saving vehicles. In 2020, in Beijing, Shanghai, and Guangzhou EVs will need to use less than 13, 18, and 20 kWh/100 km, respectively, to match the 137 gCO2/km WTW emissions for energy saving vehicles. EVs currently demonstrated in China use 24-32 kWh/100 km. Electrification will reduce petroleum imports; however, it will be very challenging for EVs to contribute to government targets for GHGs emissions reduction.
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Electricidad , Fuentes Generadoras de Energía , Gases/análisis , Efecto Invernadero , Emisiones de Vehículos/análisis , Dióxido de Carbono/análisis , China , Carbón Mineral , Suministros de Energía Eléctrica , Gasolina/análisis , Gobierno , Centrales EléctricasRESUMEN
Soluble organometallic polymers containing zirconium and silicon were synthesized by a salt metathesis reaction. The molecular weight of the polymers was measured by GPC and the corresponding structures were identified by (13)C NMR and FT-IR. After heat treatment of the polymers under argon at 1400 °C for 2 h, ZrC/SiC composites with different molar ratios of crystalline phases were obtained and characterized by XRD, elemental analysis, SEM and Raman spectroscopy. The crystalline size of the composites was approximately 100 nm-200 nm and the elements were well distributed at the different sites in the ceramics. The Raman results indicated that the ceramic residue could be considered as ZrC/SiC/C ternary composites.