RESUMEN
Eukaryotic communities in groundwater may be particularly sensitive to disturbance because they are adapted to stable environmental conditions and often have narrow spatial distributions. Traditional methods for characterising these communities, focussing on groundwater-inhabiting macro- and meiofauna (stygofauna), are challenging because of limited taxonomic knowledge and expertise (particularly in less-explored regions), and the time and expense of morphological identification. The primary objective of this study was to evaluate the vulnerability of eukaryote communities in shallow groundwater to mine water discharge containing elevated concentrations of magnesium (Mg) and sulfate (SO4). The study was undertaken in a shallow sand bed aquifer within a wet-dry tropical setting. The aquifer, featuring a saline mine water gradient primarily composed of elevated Mg and SO4, was sampled from piezometers in the creek channel upstream and downstream of the mine water influence during the dry season when only subsurface water flow was present. Groundwater communities were characterised using both morphological assessments of stygofauna from net samples and environmental DNA (eDNA) targeting the 18S rDNA and COI mtDNA genes. eDNA data revealed significant shifts in community composition in response to mine waters, contrasting with findings from traditional morphological composition data. Changes in communities determined using eDNA data were notably associated with concentrations of SO42-, Mg2+ and Na+, and water levels in the piezometers. This underscores the importance of incorporating molecular approaches in impact assessments, as relying solely on traditional stygofauna sampling methods in similar environments may lead to inaccurate conclusions about the responses of the assemblage to studied impacts.
Asunto(s)
Monitoreo del Ambiente , Agua Subterránea , Minería , Agua Subterránea/química , Eucariontes , Ríos/química , Contaminantes Químicos del Agua/análisis , Aguas SalinasRESUMEN
Environmental exposure data are used by decision-makers to assess environmental risks and implement actions to mitigate risks from contaminants. The first article in this series summarized the available evaluation schemes for environmental exposure data, of which there are few compared to those available for environmental hazard data. The second article covered the assessment of the reliability of environmental exposure data sets under the Criteria for the Reporting and Evaluation of Exposure Data (CREED). The aim of this article is to provide an overview and practical guidance on the relevance assessment in the context of the CREED approach for evaluating exposure monitoring data sets. Systematically considering relevance is critical for both evaluating existing data sets and for optimizing the design of new monitoring studies. Relevance is defined here as the degree of suitability or appropriateness of a data set to address a specific purpose or to answer the questions that have been defined by the assessor or for those generating exposure data. The purpose definition will be the foundation for the relevance assessment, to clarify how the assessor should rate the assessment criteria (fully met, partly met, not met/inappropriate, not reported, not applicable). This will provide transparency for anyone reviewing the outcomes. An explicit gap analysis (i.e., an articulation of the data set limitations for the stated purpose) is an important outcome of the relevance assessment. The relevance evaluation approach is demonstrated with three case studies, all relating to the freshwater aquatic environment, where the data sets are scored as relevant with or without restrictions, not relevant, or not assignable. The case studies represent both organic and inorganic constituents, and have different data characteristics (e.g., percentage of censored data, sampling frequencies, relation to supporting parameters). Integr Environ Assess Manag 2024;20:1004-1018. © 2023 SETAC.
Asunto(s)
Exposición a Riesgos Ambientales , Monitoreo del Ambiente , Monitoreo del Ambiente/métodos , Exposición a Riesgos Ambientales/estadística & datos numéricos , Medición de Riesgo/métodos , Contaminantes Ambientales/análisis , Reproducibilidad de los ResultadosRESUMEN
Zinc (Zn) is a metal of potential concern for a uranium mine whose receiving waters are in the World-Heritage listed Kakadu National Park in northern Australia. The chronic toxicity of Zn was assessed using seven tropical species in extremely soft freshwater from a creek upstream of the mine. Sensitivity to Zn was as follows (most sensitive to least sensitive based on 10% effect concentrations [EC10s]): mussel Velesunio angasi > gastropod Amerianna cumingi > fish Mogurnda mogurnda > cladoceran Moinodaphnia macleayi > green hydra Hydra viridissima > green alga Chlorella sp. > duckweed Lemna aequinoctialis, with EC10s (<0.45 µm filtered fraction) ranging from 21 to 320 µg/L Zn and EC50s ranging from 52 to 1867 µg/L Zn. These data were used to inform the risk assessment for the rehabilitation of the mine-site and contribute to the global Zn dataset. Environ Toxicol Chem 2023;42:679-683. © 2023 Commonwealth of Australia. Environmental Toxicology and Chemistry © 2023 SETAC.
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Bivalvos , Chlorella , Contaminantes Químicos del Agua , Animales , Zinc , Dureza , Agua Dulce , Contaminantes Químicos del Agua/toxicidadRESUMEN
Macroinvertebrates can be highly sensitive to elevated salinity in freshwater environments, and are known to respond to saline discharges. Magnesium (Mg) is a mine-related contaminant and is a potential environmental risk to a seasonally-flowing, receiving water stream in Kakadu National Park, located in the wet-dry tropics of Australia. The macroinvertebrate assemblage in the stream in the was characterised at four hydrographic phases, from early wet season flow to early dry season pools at flow cessation. On each of the four occasions representing the respective phases, individuals from the most abundant macroinvertebrate species present were collected and acutely exposed to a range (up to 19) of Mg concentrations under laboratory conditions. Sensitivity of taxa to Mg ranged between 39 mg/L Mg (Caenidae: Tasmanocoenis spp.) and 4400 mg/L Mg (Dytiscidae: Clypeodytes feryi), based on the 50% Lethal Concentration (LC50). Characterisation of the macroinvertebrate assemblage at each hydrographic phase indicated the seasons when Mg-sensitive species were present. Whilst no statistical differences in measures of seasonal sensitivity were found, the macroinvertebrate assemblages present during the early flow period had higher Mg-sensitivity than the assemblages present during other hydrographic phases. This could be attributed to the greater relative proportions of Mg-sensitive taxa (e.g. Ephemeroptera) present at early flow compared to greater relative proportions of more Mg-tolerant taxa (C. feryi and Hydacarina spp.) present during later hydrograph phases, especially periods of lower, or no, flow.
Asunto(s)
Invertebrados , Ríos , Humanos , Animales , Estaciones del Año , Invertebrados/fisiología , Magnesio , Monitoreo del Ambiente , EcosistemaRESUMEN
Copper (Cu) is a contaminant of potential concern for a uranium mine whose receiving waters are in the World Heritage-listed Kakadu National Park in northern Australia. The physicochemical characteristics of the freshwaters in this region enhance metal bioavailability and toxicity. Seven tropical species were used to assess the chronic toxicity of Cu in extremely soft freshwater from a creek upstream of the mine. Sensitivity to Cu was as follows: Moinodaphnia macleayi > Chlorella sp. > Velesunio sp. > Hydra viridissima > Amerianna cumingi > Lemna aequinoctialis > Mogurnda mogurnda. The 10% effect concentrations (EC10s) ranged from 1.0 µg/L Cu for the cladoceran Moinodaphnia macleayi to 9.6 µg/L for the fish M. mogurnda. The EC50s ranged from 6.6 µg/L Cu for the mussel Velesunio sp. to 22.5 µg/L Cu for M. mogurnda. Geochemical modeling predicted Cu to be strongly bound to fulvic acid (80%-99%) and of low bioavailability (0.02%-11.5%) under these conditions. Protective concentrations (PCs) were derived from a species sensitivity distribution for the local biota. The 99% PC (PC99), PC95, PC90, and PC80 values were 0.5, 0.8, 1.0, and 1.5 µg/L Cu, respectively. These threshold values suggest that the current Australian and New Zealand default national 99% protection guideline value for Cu (1.0 µg/L) would not provide adequate protection in freshwaters of low hardness, particularly for this area of high conservation value. The continuous criterion concentration predicted by the Cu biotic ligand model for conditions of low pH (6.1), low dissolved organic carbon (2.5 mg/L), low hardness (3.3 mg/L), and 27 °C was 0.48 µg/L Cu, comparable with the PC99. Consideration of the natural water quality conditions of a site is paramount for protective water quality guidelines. Environ Toxicol Chem 2022;41:2808-2821. © 2022 Commonwealth of Australia. Environmental Toxicology and Chemistry © 2022 SETAC.
Asunto(s)
Bivalvos , Chlorella , Cladóceros , Perciformes , Uranio , Contaminantes Químicos del Agua , Animales , Cobre/toxicidad , Uranio/toxicidad , Dureza , Ligandos , Contaminantes Químicos del Agua/análisis , Australia , Agua DulceRESUMEN
CONTEXT: Ecological Risk Assessments (ERAs) are important tools for supporting evidence-based decision making. However, most ERA frameworks rarely consider complex ecological feedbacks, which limit their capacity to evaluate risks at community and ecosystem levels of organisation. METHOD: We used qualitative mathematical modelling to add additional perspectives to previously conducted ERAs for the rehabilitation of the Ranger uranium mine (Northern Territory, Australia) and support an assessment of the cumulative risks from the mine site. Using expert elicitation workshops, separate qualitative models and scenarios were developed for aquatic and terrestrial systems. The models developed in the workshops were used to construct Bayes Nets that predicted whole-of-ecosystem outcomes after components were perturbed. RESULTS: The terrestrial model considered the effect of fire and weeds on established native vegetation that will be important for the successful rehabilitation of Ranger. It predicted that a combined intervention that suppresses both weeds and fire intensity gave similar response predictions as for weed control alone, except for lower levels of certainty to tall grasses and fire intensity in models with immature trees or tall grasses. However, this had ambiguous predictions for short grasses and forbs, and tall grasses in models representing mature vegetation. The aquatic model considered the effects of magnesium (Mg), a key solute in current and predicted mine runoff and groundwater egress, which is known to adversely affect many aquatic species. The aquatic models provided support that attached algae and phytoplankton assemblages are the key trophic base for food webs. It predicted that shifts in phytoplankton abundance arising from increase in Mg to receiving waters, may result in cascading effects through the food-chain. CONCLUSION: The qualitative modelling approach was flexible and capable of modelling both gradual (i.e. decadal) processes in the mine-site restoration and the comparatively more rapid (seasonal) processes of the aquatic ecosystem. The modelling also provides a useful decision tool for identifying important ecosystem sub-systems for further research efforts.
Asunto(s)
Ecosistema , Uranio , Teorema de Bayes , Cadena Alimentaria , Medición de Riesgo , Uranio/análisisRESUMEN
The derivation of sediment quality guideline values (SQGVs) presents significant challenges. Arguably the most important challenge is to conduct toxicity tests using contaminated sediments with physico-chemistry that represents real-world scenarios. We used a novel metal spiking method for an experiment that ultimately aims to derive a uranium SQGV. Two pilot studies were conducted to inform the final spiking design, i.e. percolating a uranyl sulfate solution through natural wetland sediments. An initial pilot study that used extended mixing equilibration phases produced hardened sediments not representative of natural sediments. A subsequent percolation method produced sediment with similar texture to natural sediment and was used as the method for spiking the sediments. The range of total recoverable uranium (TR-U) concentrations achieved was 8-3200 mg/kg. This reflected the concentrations found in natural wetlands and water management ponds found on a uranium mine site and was above natural levels. Dilute-acid extractable uranium (AE-U) concentrations were >80% of total concentrations, indicating that much of the uranium in the spiked sediment was labile and potentially bioavailable. The portion of TR-U extractable as AE-U was similar at the start and end of the 4.5-month field-deployment. Porewater uranium (PW-U) analyses indicated that partition coefficients (Kd) were 2000-20,000 L/kg, and PW-U was greater in post- than pre-field-deployed samples when TR-U was ≤1500 mg/kg, indicating the binding became weaker during the field-deployment period. At higher spiked-U concentrations, the PW-U was lower post-field-deployment. Comparing the physico-chemical data of the spiked sediments with environmental monitoring data from sediments in the vicinity of a uranium mining operation indicated that they were representative of sediments contaminated by mining and that the U-spiked sediments had a clear U concentration gradient. This confirmed the suitability of the spiking procedure for preparing sediments that were suitable for deriving a SQGV for uranium.
Asunto(s)
Uranio , Contaminantes Radiactivos del Agua , Sedimentos Geológicos , Proyectos Piloto , Uranio/análisis , Contaminantes Radiactivos del Agua/análisis , HumedalesRESUMEN
Bacteria and archaea (prokaryotes) are vital components for maintaining healthy function of groundwater ecosystems. The prokaryotic community composition and associated putative functional processes were examined in a shallow sandy aquifer in a wet-dry tropical environment. The aquifer had a contaminated gradient of saline mine-water, which primarily consisted of elevated magnesium (Mg2+) and sulfate (SO42-), although other major ions and trace metals were also present. Groundwaters were sampled from piezometers, approximately 2 m in depth, located in the creek channel upstream and downstream of the mine-water influence. Sampling occurred during the dry-season when only subsurface water flow was present. Next generation sequencing was used to analyse the prokaryote assemblages using 16S rDNA and metabolic functions were predicted with FAPROTAX. Significant changes in community composition and functional processes were observed with exposure to mine-waters. Communities in the exposed sites had significantly lower relative abundance of methanotrophs such as Methylococcaceae and methanogens (Methanobacteriaceae), but higher abundance in Nitrososphaeraceae, associated with nitrification, indicating potentially important changes in the biogeochemistry of the exposed sites. The changes were most strongly correlated with concentrations of SO42-, Mg2+ and Na+. This knowledge allows an assessment of the risk of mine-water contamination to groundwater ecosystem function and aids mine-water management.
Asunto(s)
Ecosistema , Agua Subterránea , Archaea/genética , Bacterias/genética , AguaRESUMEN
Six tropical freshwater species were used to assess the toxicity of mine waters from a uranium mine adjacent to a World Heritage area in northern Australia. Key contaminants of potential concern for the mine were U, Mg, Mn, and total ammonia nitrogen (TAN). Direct toxicity assessments were carried out to assess whether the established site-specific guideline values for individual contaminants would be protective with the contaminants occurring as mixtures. Metal speciation was calculated for contaminants to determine which were the major contributors of toxicity, with 84 to 96% of Mg predicted in the free-ion form as Mg2+ , and 76 to 92% of Mn predicted as Mn2+ . Uranium, Al, and Cu were predicted to be strongly bound to fulvic acid. Uranium, Mg, Mn, and Cu were incorporated into concentration addition or independent action mixture toxicity models to compare the observed toxicity in each of the waters with predicted toxicity. For >90% of the data, mine-water toxicity was less than predicted by the concentration addition model. Instances where toxicity was greater than predicted were accompanied by exceedances of individual metal guideline values in all but one case (i.e., a Mg concentration within 10% of the guideline value). This indicates that existing individual water quality guideline values for U, Mg, Mn, and TAN would adequately protect ecosystems downstream of the mine. Environ Toxicol Chem 2021;40:2334-2346. © 2021 Commonwealth of Australia. Environmental Toxicology and Chemistry © 2021 SETAC.
Asunto(s)
Uranio , Contaminantes Químicos del Agua , Amoníaco , Ecosistema , Agua Dulce , Uranio/análisis , Uranio/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad , Calidad del AguaRESUMEN
Many international guidance documents for deriving water quality guideline values recommend the use of chronic toxicity data. For the tropical fish northern trout gudgeon, Mogurnda mogurnda, 96-h acute and 28-d chronic toxicity tests have been developed, but both tests have drawbacks. The 96-h toxicity test is acute and has a lethal endpoint; hence it is not a preferred method for guideline value derivation. The 28-d method has a sublethal (growth) endpoint, but is highly resource intensive and is high risk in terms of not meeting quality control criteria. The present study aimed to determine the feasibility of a 7-d larval growth toxicity test as an alternative to the 96-h survival and 28-d growth tests. Once the method was successfully developed, derived toxicity estimates for uranium, magnesium, and manganese were compared with those for other endpoints and tests lengths within the literature. As a final validation of the 7-d method, the sensitivity of the 7-d growth endpoint was compared with those of 14-, 21-, and 28-d exposures. Fish growth rate, based on length, over 7 d was significantly more sensitive compared with existing acute toxicity endpoints for magnesium and manganese, and was similarly sensitive to existing chronic toxicity endpoints for uranium. For uranium, the sensitivity of the growth endpoint over the 4 exposure periods was similar, suggesting that 7 d as an exposure duration is sufficient to provide an indication of longer term chronic growth effects. The sensitivity of the 7-d method, across the 3 metals tested, highlights the benefit of utilizing the highly reliable short-term 7-d chronic toxicity test method in future toxicity testing using M. mogurnda. Environ Toxicol Chem 2021;40:1596-1605. © 2021 Commonwealth of Australia. Environmental Toxicology and Chemistry © 2021 SETAC.
Asunto(s)
Uranio , Contaminantes Químicos del Agua , Animales , Magnesio , Manganeso/toxicidad , Pruebas de Toxicidad Crónica , Trucha , Uranio/análisis , Uranio/toxicidad , Contaminantes Químicos del Agua/toxicidadRESUMEN
Magnesium (Mg) is a mining-related contaminant in the Alligators Rivers Region of tropical northern Australia. A mesocosm experiment was used to assess Mg toxicity to aquatic freshwater assemblages. Twenty-five 2700-L tubs were arranged, stratified randomly, on the bed of Magela Creek, a seasonally flowing, sandy stream channel in the Alligator Rivers Region of northern Australia. The experiment comprised 5 replicates of 4 nominal Mg treatments, 2.5, 7.5, 23, and 68 mg L-1 , and a control. Phytoplankton biomass, and diatom, zooplankton, and macroinvertebrate assemblages present in the treatment tubs were sampled before and after Mg addition. A significant negative relationship between phytoplankton biomass and Mg was observed 4 wk after Mg addition as measured by chlorophyll a concentrations (r2 = 0.97, p = 0.01). This result was supported by reductions in some major phytoplankton groups in response to increasing Mg concentrations, in the same experiment and from independent field studies. There was a significant negative relationship between zooplankton assemblage similarity (to control) and Mg concentrations (r2 = 0.96, p = 0.002). Seven weeks after Mg addition, macroinvertebrate assemblages were dominated by 3 microcrustacean groups (Ostracoda, Cladocera, and Copepoda), each reaching maximum abundance at intermediate Mg concentrations (i.e., unimodal responses). The responses of phytoplankton and zooplankton were used to derive assemblage effect concentrations (Mg concentrations resulting in x% of the assemblage change [ECx]). Magnesium concentrations resulting in assemblage EC01 values were <3 mg L-1 . Together with candidate guideline values from other laboratory- and field-based lines of evidence, the mesocosm EC01 values were incorporated into a weight-of-evidence framework for a robust regulatory approach to environmental protection. Environ Toxicol Chem 2020;39:1973-1987. © 2020 Commonwealth of Australia. Published by Wiley Periodicals LLC on behalf of SETAC.
Asunto(s)
Crustáceos/crecimiento & desarrollo , Magnesio/toxicidad , Fitoplancton/crecimiento & desarrollo , Ríos/química , Contaminantes Químicos del Agua/toxicidad , Zooplancton/crecimiento & desarrollo , Animales , Australia , Biomasa , Clorofila A/análisis , Cladóceros/crecimiento & desarrollo , Copépodos/crecimiento & desarrollo , Diatomeas/crecimiento & desarrollo , Relación Dosis-Respuesta a Droga , Magnesio/análisis , Modelos Teóricos , Especificidad de la Especie , Contaminantes Químicos del Agua/análisisRESUMEN
Magnesium (Mg) is a common contaminant in mine water discharges. Although Mg is an essential element in biological processes, increased concentrations from anthropogenic sources can stress aquatic ecosystems. Additionally, studies evaluating the effects of Mg on north Australian freshwater species have indicated that in very soft waters there is a high risk to some species. Freshwater mussels are an ecologically and culturally important taxon in many freshwater environments, but knowledge of their sensitivity to Mg is limited. In the present study, the acute and chronic sensitivity of two freshwater mussel species, Velesunio angasi and an undescribed Velesunio species, to Mg was assessed (using MgSO4) on their early life stages, larval glochidia and post-parasitic juveniles. Acute 24-h exposures with glochidia generated a mean median lethal (LC50) toxicity estimate of 284mg/L for the five tests with V. angasi, and a mean LC50 of 300mg/L for the three tests with Velesunio sp. Mean chronic 14-d toxicity estimates resulting in 50% (EC50) and 10% (EC10) growth rate reductions for juveniles were 241 and 88mg/L respectively for the three tests with V. angasi juveniles, and 232 and 87mg/L respectively for the three tests with Velesunio sp. juveniles. The results represent the first acute and chronic Mg toxicity data for tropical freshwater mussels, and indicated that V. angasi and Velesunio sp. exhibited similar sensitivity and were moderately sensitive to Mg when compared to other tropical species. These results are a valuable contribution to the small existing dataset for Mg toxicity to tropical freshwater species, which can be used to inform water management in areas where Mg is a contaminant of concern, and ensure the protection of these taxa.
Asunto(s)
Bivalvos/efectos de los fármacos , Agua Dulce/química , Larva/efectos de los fármacos , Magnesio/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Australia , Ecosistema , Dosificación Letal Mediana , Magnesio/análisis , Alimentos Marinos , Pruebas de Toxicidad Aguda , Pruebas de Toxicidad Crónica , Contaminantes Químicos del Agua/análisisRESUMEN
Existing prescriptive guidance on the derivation of local water quality benchmarks (WQBs; e.g., guideline values, criteria, standards) for protecting aquatic ecosystems is limited to only 3 to 4 specific approaches. These approaches do not represent the full suite available for deriving local WQBs for multiple types of water quality-related issues. The general lack of guidance is inconsistent with the need for, and benefits of, local WQBs, and can constrain the appropriate selection and subsequent evaluation of derivation approaches. Consequently, the defensibility of local WQBs may not be commensurate with the nature of the issues for which they are derived. Moreover, where local WQBs are incorporated into regulatory requirements, the lack of guidance presents a potential risk to the derivation of appropriate WQBs and the achievement of desired environmental outcomes. This review addresses the deficiency in guidance by 1) defining local WQBs and outlining initial considerations for deciding if one is required; 2) summarizing the existing regulatory context; 3) summarizing existing guidance and identifying gaps; 4) describing strengths, weaknesses, and potential applications of a range of derivation approaches based on laboratory and/or field data; and 5) presenting a conceptual framework for appropriately selecting and evaluating a derivation approach to best suit the need. The guidance incorporates an existing set of guiding principles for deriving local WQBs and reinforces an existing categorization of site-adapted and site-specific WQBs. The conceptual framework recognizes the need to strike an appropriate balance between effort and ecological risk and, thus, embeds the concept of fit-for-purpose by considering both the significance of the issue being assessed and the extent to which the approach provides confidence that the ecosystem will be appropriately protected. The guidance can be used by industry, regulators, and others for both the a priori selection and the post hoc evaluation of appropriate approaches for deriving local WQBs. Integr Environ Assess Manag 2019;15:683-702. © 2019 The Authors.
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Benchmarking/métodos , Contaminantes Químicos del Agua/análisis , Calidad del Agua/normas , Benchmarking/normasRESUMEN
Freshwater mussels play key roles in aquatic ecosystems, but are experiencing a global decline. Although studies have reported high acute sensitivity of mussels to some contaminants, chronic toxicity data are lacking for deriving high-reliability water quality guideline values. Ammonia is a contaminant of potential concern in some catchments of tropical northern Australia, where freshwater mussels are important ecological and cultural components. The extremely soft waters (hardness < 5 mg/L) of these environments can result in increased toxicity of many contaminants including ammonia, and regionally relevant tropical guideline values are needed to adequately protect these unique ecosystems. An optimized 14-d toxicity test protocol was used to assess the chronic toxicity of ammonia for 2 species, the lotic Velesunio sp. and the lentic Velesunio angasi. Ammonia exposures were conducted at pH 6.0 and 27 ± 0.5 °C to represent local environmental conditions, using shell length growth rate as the endpoint. Chronic toxicity estimates indicated high sensitivity to ammonia, with mean median effect concentrations (in total ammonia nitrogen) being 7.0 mg/L for V. angasi from the semi-urbanized Lake Bennett, 9.2 mg/L for V. angasi from Sandy Billabong, and 11.3 mg/L for Velesunio sp. from Gulungul Creek. When the 10% effect concentration values were compared with other chronic ammonia data (normalized to pH 7.0 and 20 °C), Velesunio spp. were found to be more sensitive than 8 of 16 other temperate and 7 of 9 tropical invertebrate and fish species. These chronic toxicity estimates will be used to further inform regionally relevant and site-specific guideline values. Environ Toxicol Chem 2019;38:841-851. © 2019 Commonwealth of Australia. Published by Wiley Periodicals Inc. on behalf of SETAC.
Asunto(s)
Amoníaco/toxicidad , Bivalvos/efectos de los fármacos , Agua Dulce/química , Contaminantes Químicos del Agua/toxicidad , Calidad del Agua , Amoníaco/análisis , Animales , Australia , Bivalvos/crecimiento & desarrollo , Ecosistema , Guías como Asunto , Reproducibilidad de los Resultados , Pruebas de Toxicidad Crónica , Clima Tropical , Contaminantes Químicos del Agua/análisisRESUMEN
Magnesium (Mg) is a primary contaminant in mine water discharges from the Ranger Uranium Mine (north Australia). Site-specific water quality guideline values (WQGVs) for Mg have been derived from laboratory and field studies. Contaminated groundwater with elevated electrical conductivity and metals (Mg, Mn, U, SO4 , and Ca) was detected flowing from the mine site into adjacent surface waters. This provided an opportunity to investigate the protectiveness of the Mg WQGV by conducting an integrated laboratory and field study. A direct toxicity assessment (DTA) of the groundwater was conducted with local tropical freshwater species: duckweed (Lemna aequinoctialis), green hydra (Hydra viridissima), and the aquatic snail Amerianna cumingi. An in situ toxicity assessment was carried out in the creek receiving diluted groundwater by use of the same species of snail, to aid interpretation of laboratory-derived data. The toxicity of the contaminated groundwater was higher than Mg-only toxicity testing for H. viridissima, with other elevated metals and major ions contributing to toxicity. However, for duckweed and snail, the contaminated groundwater was less toxic than the Mg-only testing. In situ snail monitoring supported laboratory exposures, showing no effect on reproduction of A. cumingi exposed to an average of approximately 5 mg/L Mg; however, a very small effect was noted closer to the groundwater source, probably associated with other contaminants. The minimal toxicity observed for L. aequinoctialis and A. cumingi, despite the elevated Mg, can be explained by the high calcium (Ca) concentration of the water and the potential amelioration of metal toxicity. The extent of Ca amelioration of Mg toxicity was organism dependent. This study affirms the proposed environmental rehabilitation standard of 3 mg/L Mg for surface waters with a Ca concentration typical of water from this mine site. Integr Environ Assess Manag 2019;15:64-76. © 2018 SETAC.
Asunto(s)
Magnesio/análisis , Uranio/análisis , Contaminantes Radiactivos del Agua/análisis , Araceae , Australia , Minería , Pruebas de Toxicidad , Uranio/toxicidad , Contaminantes Radiactivos del Agua/toxicidadRESUMEN
The chronic toxicity of ammonia to tropical freshwater species is understudied, and thus data on temperate species have been used to derive water quality guideline values for tropical regions. Such practices may lead to underprotective guideline values due to differences in toxicities observed between tropical and temperate species. In addition, the presence of ammonia in low-ionic-strength waters may also result in higher toxicity, and studies on this factor are limited. The present study assessed the toxicity of ammonia to 6 tropical freshwater species in low-ionic-strength waters. Because ammonia toxicity varies depending on the pH and temperature, test water pH concentrations were maintained at approximately pH 6.0 ± 0.3 at temperatures between 27.5 and 30 °C. Low-effect chronic inhibition concentrations were derived for the following species: Chlorella sp. 66 mg L-1 ; Lemna aequinoctialis 22 mg L-1 ; Hydra viridissima 1.8 mg L-1 ; Moinodaphnia macleayi 27 mg L-1 ; Amerianna cumingi 17 mg L-1 ; and Mogurnda mogurnda 5.4 mg L-1 total ammonia nitrogen. Two of the species tested (a cnidarian and a fish species) were among the most sensitive reported anywhere within their taxonomic group. Chronic ammonia datasets representing toxicity estimates for temperate and tropical species were plotted and compared using species sensitivity distributions. The results indicate that the differences in chronic toxicity observed between tropical and temperate species were likely due to the low ionic strength of the waters to which tropical species were exposed, rather than any inherent physiological differences between species from tropical and temperate regions. This finding suggests that tropical waters of low ionic strength may be at a higher risk from ammonia compared with other freshwater ecosystems. Environ Toxicol Chem 2019;38:177-189. © 2018 Commonwealth of Australia. Published by Wiley Periodicals, Inc. on behalf of SETAC.
Asunto(s)
Amoníaco/toxicidad , Agua Dulce/química , Pruebas de Toxicidad Crónica , Clima Tropical , Animales , Araceae/efectos de los fármacos , Australia , Chlorella/efectos de los fármacos , Cladóceros/efectos de los fármacos , Hydra/efectos de los fármacos , Perciformes , Control de Calidad , Especificidad de la Especie , Contaminantes Químicos del Agua , Calidad del AguaRESUMEN
A novel bioassay is presented that allows for the estimation of the chronic toxicity of contaminants in receiving tropical marine environments. Relevant procedures to identify contaminants of concern and evaluate hazards associated with contamination in these environments have long remained inadequate. The 6-day bioassay is conducted using freshly hatched planktonic larvae of the hermit crab Coenobita variabilis and is targeted at generating environmentally relevant, chronic toxicity data. The developmental endpoint demonstrated consistently high control performance and was validated through the use of copper as a reference toxicant. In addition, the biological effects of aluminium, gallium and molybdenum were assessed. The endpoint expressed high sensitivity to copper (EC10 =â¯24⯵gâ¯L-1) and moderate sensitivity to aluminium (EC10 =â¯312⯵gâ¯L-1), whereas gallium and molybdenum elicited no obvious effects, even at high concentrations (EC10 >â¯6000⯵gâ¯L-1), providing valuable information on the toxicity of these elements in tropical marine waters for derivation of water quality guidelines or testing of compliance limits.
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Aluminio/toxicidad , Anomuros , Bioensayo/métodos , Cobre/toxicidad , Galio/toxicidad , Molibdeno/toxicidad , Animales , Larva/efectos de los fármacos , Larva/crecimiento & desarrollo , Contaminantes Químicos del Agua/toxicidadRESUMEN
Revised water quality guideline values (WQGVs) are presented for the metals aluminium (Al), gallium (Ga) and molybdenum (Mo) in receiving marine environments. These elements are commonly found in elevated concentrations in alumina refinery waste streams, yet current WQGVs fail to accurately assess the environmental risk. Here, chronic biological effects data we have generated over the course of several years were combined with toxicity data from the open literature to construct species sensitivity distributions (SSDs) which enabled the computation of revised WQGVs for Al, Ga and Mo in marine environments. These procedures are in accordance with internationally recommended derivation procedures, and newly computed WQGVs may be incorporated in regulatory frameworks aimed at sustainable exploitation of environmental resources and ongoing protection of the marine estate. Where the available datasets allowed such distinction, separate SSDs were constructed for temperate and tropical environments and zone-specific WQGVs derived. Extrapolated from the SSDs, WQGVs of 56 µg Al L-1, 800 µg Ga L-1 and 3.88 mg Mo L-1 (in the 0.45-µm filtered fraction) for 95% species protection were recommended for implementation in both temperate and tropical receiving environments. Currently, there is insufficient validation to separate the tropical from the temperate data and in most cases, application of the generic WQGVs is recommended.
Asunto(s)
Aluminio/análisis , Monitoreo del Ambiente/métodos , Galio/análisis , Molibdeno/análisis , Contaminantes Químicos del Agua/análisis , Calidad del Agua/normas , Aluminio/toxicidad , Animales , Organismos Acuáticos/efectos de los fármacos , Organismos Acuáticos/crecimiento & desarrollo , Galio/toxicidad , Guías como Asunto , Molibdeno/toxicidad , Agua de Mar/química , Especificidad de la Especie , Pruebas de Toxicidad Crónica , Clima Tropical , Contaminantes Químicos del Agua/toxicidadRESUMEN
Managing saline water discharges from mining operations is a global environmental challenge. Measuring the location and extent of surface efflorescence can indicate solute movement before changes in electrical conductivity (EC) are detected in waterways. We hypothesised through the use of a case study that ground-based reflectance spectrometry and airborne hyperspectral (450-2500â¯nm) analysis of surface efflorescence could be a rapid method for monitoring large regions of the surrounding environment, including downstream of remote mines. X-ray diffraction and X-ray fluorescence were used to determine mineralogy and elemental composition of surface salts around a uranium mine. Salt samples were found to be mixtures of magnesium sulfate. The reflectance of field spectra varied depending on the hydration of the mineral, mainly hexahydrite and starkeyite. A constrained energy minimisation technique was used to match the field reflectance spectra to the airborne data. Airborne matches were confirmed at the field sampling sites and surrounds. Salts were also detected at lower matches at mine water irrigation areas where excess mine water had previously been applied. Hence, hyperspectral remote sensing is a potentially rapid and sensitive method for mapping magnesium sulfates over large areas in operating and rehabilitated mines. It was successfully demonstrated as a tool for monitoring and assessment of efflorescence as a result of saline processes.
RESUMEN
Ammonia is recognized as a major pollutant worldwide, originating from natural and anthropogenic sources. Studies have reported that freshwater mussels are among the most sensitive taxa to ammonia, but few data are available on ammonia toxicity for the early life stages of freshwater mussels from tropical regions. We report on the modification of a 24-h acute toxicity test protocol for tropical freshwater mussels and application of the test using ammonia. Velesunio spp. from 3 different sites were used to assess the toxicity of ammonia at a targeted pH of 6.0 and a water temperature of 27.5 °C, which were the average annual values for some slightly to moderately acidic, soft water (3-6 mg/L as CaCO3 ) creeks of tropical northern Australia. The valve closure responses of mussel glochidia (larvae) to a sodium chloride solution were used to measure the survival endpoint. Acute toxicity estimates indicate that tropical Velesunio spp. were highly sensitive to ammonia, with 24-h exposures to ammonium sulfate generating median lethal concentration estimates ranging from 6.8 to 14.2 mg/L total ammonia nitrogen, which, when adjusted to pH 7 and 20 °C, were among the highest sensitivities yet reported for any freshwater mussel species, and among the highest in sensitivity for any tropical taxon. These toxicity estimates can contribute to the derivation or refinement of ammonia guideline values for freshwater ecosystems globally. Environ Toxicol Chem 2018;37:2175-2187. © 2018 SETAC.