Interactions of human MCM2-7 proteins with the proteins of TIM, TIPIN, an amino-terminal fragment of Rb, and p27 were examined by co-immuno-precipitation experiment using cell lysates of co-expressed insect cells. TIM and TIPIN, both of which are involved in regulation of DNA replication fork progression, mainly interacted with MCM3-7 proteins. The amino-terminal fragment of Rb, which inhibits DNA replication in Xenopus egg extracts, was able to bind with MCM3 and MCM6 proteins in addition to MCM7 protein. In contrast, p27 was not able to bind any MCM2-7 proteins under the comparable conditions. These results indicate that the proteins, which are known to interact with MCM proteins, bind with MCM2-7 proteins with different affinities and specificities.
DNA Replication , Animals , Carrier Proteins/metabolism , Cell Cycle Proteins/metabolism , Cyclin-Dependent Kinase Inhibitor p27 , DNA Helicases/metabolism , DNA-Binding Proteins/metabolism , E2F1 Transcription Factor/metabolism , Humans , Intracellular Signaling Peptides and Proteins/metabolism , Minichromosome Maintenance Complex Component 2 , Minichromosome Maintenance Complex Component 3 , Minichromosome Maintenance Complex Component 4 , Minichromosome Maintenance Complex Component 6 , Minichromosome Maintenance Complex Component 7 , Nuclear Proteins/metabolism , Protein Binding , Recombinant Proteins/chemistry , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Xenopus
Crohn's disease is a chronic inflammatory bowel disease characterized by discontinuous chronic inflammation that may affect virtually all organs, including the head and neck. Laryngeal involvement in Crohn's disease is very rare, and only 9 cases have been reported. All 9 patients complained of difficulty in breathing due to edema and ulceration from the larynx to the hypopharynx. The present patient was a 31-year-old woman who had experienced the intestinal symptoms of Crohn's disease starting 20 months earlier and complained of hoarseness, sore throat, and odynophagia. The hoarseness worsened gradually because of limited ulceration of the vocal fold without edema. We describe the first case in which limited ulceration occurred on the vocal fold without airway involvement.
Crohn Disease/pathology , Hoarseness/etiology , Vocal Cords/pathology , Adult , Crohn Disease/complications , Crohn Disease/therapy , Female , Hoarseness/pathology , Hoarseness/therapy , Humans , Recurrence
Mohs' chemosurgery, originally developed to treat skin cancer, uses zinc chloride in Mohs' ointment to fix tissues, and is applicable in different clinical settings. In advanced head and neck cancer, Mohs' chemosurgery relieves main skin-infiltration symptoms such as bleeding, infection, exudation, and severe pain. Mohs' chemosurgery conducted in two cases of advanced head and neck cancer yielded an acceptable result free of bleeding, pain, exudation, and infection. Steps in palliative care are repeated until the tumor surface is completely fixed. Using Mohs' ointment provides acceptable relief without technical complications. Although not a topical chemotherapeutic agent, it fixes the lesion well.
Chlorides/administration & dosage , Head and Neck Neoplasms/drug therapy , Skin Neoplasms/drug therapy , Zinc Compounds/administration & dosage , Humans , Male , Middle Aged , Ointments , Palliative Care
The effects of germacranolides isolated from Calea urticifolia on adipocytic differentiation of 3T3-L1 cells were examined. These germacranolides inhibited adipogenesis at a concentration of 1.25-5 microM. But no inhibitory activity against cell proliferation and no nonspecific binding activity to protein were observed. These results indicate that these germacranolides are the specific inhibitors of preadipocyte differentiation.
Adipocytes/drug effects , Asteraceae/chemistry , Cell Differentiation/drug effects , Sesquiterpenes, Germacrane/pharmacology , Stem Cells/drug effects , 3T3 Cells , Animals , Cell Proliferation/drug effects , Lipid Metabolism , Mice
Four germacranolides, named calealactones A-C and calealactone B [corrected] were isolated from the leaves of Calea urticifolia (Compositae) in addition to three known germacranolides. Their structures were established on the basis of spectroscopic analysis including by 2D NMR spectroscopy. Calealactone C and 2,3-epoxyjuanisulamin displayed potent toxicity to U937 cells.
Asteraceae/chemistry , Sesquiterpenes, Germacrane/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/pharmacology , Humans , Leukemia , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Leaves/chemistry , Sesquiterpenes, Germacrane/isolation & purification , Sesquiterpenes, Germacrane/pharmacology , U937 Cells
