Structural characterization of modified coconut husk lignin via steam explosion pretreatment as a renewable phenol substitutes.

The effects of steam explosion (SE) pretreatment on the structural properties of lignin isolated from coconut husk (CH) biomass via soda pulping were investigated in this work. The isolated SE lignin was classified as dilute acid impregnation SE lignin (ASEL), water impregnation SE lignin (WSEL), and 2-naphthol impregnation SE lignin (NSEL). The various types of functional groups isolated from SE lignin were characterized and compared using a variety of complementary analyses: FTIR spectroscopy, NMR spectroscopy, GPC chromatography, HPAEC-PAD chromatography and thermal analyses. It was revealed that ASEL has the highest solid recovery with 55.89 % yield as well as the highest sugars content compared to WSEL (45.66 % yield) and NSEL (49.37 % yield). Besides, all isolated SE lignin contain a significant quantity of non-condensed G-type and S-type units but less amount of H-type units as supported by previous research. The SE lignin produced lignin with higher molecular weight (Mw ASEL: 72725 g mol-1 > Mw WSEL: 13112 g mol-1 > Mw NSEL: 6891 g mol-1) seems to influence the success of the synthesis reaction of phenolic resins. Because of the large variances in the physicochemical properties of SE lignin polymers, their structural properties were increased toward numerous alternative techniques in lignin-based applications.

Radiological and Dosimetric Evaluation of Biomaterial Composite Phantoms with High Energy Photons and Electrons.

ABSTRACT: The current study was undertaken to investigate the radiological and dosimetric parameters of natural product-based composite (SPI/NaOH/IA-PAE/ Rhizophora spp .) phantoms. The radiological properties of the phantoms were measured at different gamma energies from Compton scatter of photons through angles of 0, 30, 45, 60, 75, and 90 degrees. Ionization chamber (IC) and Gafchromic EBT3 film dosimeters were employed to evaluate the dosimetric characteristics for photons (6-10 MV) and electrons (6-15 MeV). Radiological property results of the composite phantoms were consistent with good quality compared to those of solid water phantoms and theoretical values of water. Photon beam quality index of the SPI15 phantom with p-values of 0.071 and 0.073 exhibited insignificant changes. In addition, good agreement was found between PDD curves measured with IC and Gafchromic EBT3 film for both photons and electrons. The computed therapeutic and half-value depth ranges matched within the limits and are similar to those of water and solid water phantoms. Therefore, the radiological and dosimetric parameters of the studied composite phantom permit its use in the selection of convenient tissue- and water-equivalent phantom material for medical applications.

Dosimetric Characterization of DSF/NaOH/IA-PAE/R. spp. Phantom Material for Radiation Therapy.

Background: Different compositions of DSF/NaOH/IA-PAE/R. spp. composite particleboard phantoms were constructed. Methods: Photon attenuation characteristics were ascertained using gamma rays from 137Cs and 60Co. Absorbed doses at the location of an ionization chamber and Gafchromic EBT3 radiochromic films were calculated for high-energy photons (6 and 10 MV) and electrons (6, 9, 12, and 15 MeV). Results: The calculated TPR20,10 values indicate that the percentage discrepancy for 6 and 10 MV was in the range of 0.29-0.72% and 0.26-0.65%. It was also found that the relative difference in the dmax to water and solid water phantoms was between 1.08-1.28% and 5.42-6.70%. The discrepancies in the determination of PDD curves with 6, 9, 12, and 15 MeV, and those of water and solid water phantoms, ranged from 2.40-4.84%. Comparable results were found using the EBT3 films with variations of 2.0-7.0% for 6 and 10 MV photons. Likewise, the discrepancies for 6, 9, 12, and 15 MeV electrons were within an acceptable range of 2.0-4.5%. Conclusions: On the basis of these findings, the DSF/NaOH/IA-PAE/R. spp. particleboard phantoms with 15 wt% IA-PAE addition level can be effectively used as alternative tissue-equivalent phantom material for radiation therapy applications.

Performance of natural product-based materials as adhesives in the fabrication of mangrove wood composites.

Biodegradable adhesives prepared using three different forms of soy protein-based products (defatted soy flour/soy protein concentrate/soy protein isolate), sodium hydroxide, and itaconic acid polyamidoamine-epichlorohydrin (IA-PAE) with 0 wt%-20 wt% substitution rates were utilized to enhance the production of mangrove wood composites. 1H nuclear magnetic resonance, differential scanning calorimetry, and ultra-high-resolution field emission scanning electron microscopy were employed to characterize the composite samples. Other measurements involved the determination of viscosity, pH, physical, mechanical, dimensional stability, CT numbers, and relative electron density parameters. The ideal curing conditions for the composite bio-adhesives were found to be 15 wt% IA-PAE, 602.50 ± 172.21-391.11 ± 105.82 mPa s, pH 11.0, 180 °C, and 18 min, respectively. The improved physiochemical characteristics of DSF, SPC, and SPI confirmed that NaOH/IA-PAE was integrated into the adhesive system and ameliorated the overall performance of the resulting composites. The results showed that all composite samples, except for those bonded with 0 wt% and 5 wt% IA-PAE, matched up with the quality specification stated in the JIS A-5908 and ASTM D1037. Samples D1, D2, and D3 exhibited optimum characteristics, demonstrating their uses in the development of low-toxicity and sustainable reference tissue substitute phantom in radiological areas.

Lignin nanoparticles-reduced graphene oxide based hydrogel: A novel strategy for environmental applications.

In this work, facile fabrication of lignin nanoparticles (LNP)-based three-dimensional reduced graphene oxide hydrogel (rGO@LNP) has been demonstrated as a novel strategy for environmental applications. Herein, LNP were facilely synthesized from walnut shell waste through a direct chemical route. These LNP were incorporated into the continuous porous network of rGO network to fabricate rGO@LNP hydrogel. Characterization studies were carried out using various analytical techniques viz. scanning electron microscopy, Fourier transform IR spectroscopy, X-ray diffraction and thermogravimetric analysis. The efficiency of rGO@LNP hydrogel as adsorptive platform was evaluated by employing methylene blue and Pb2+ as model pollutants, whilst the effect of various experimental parameters was ascertained for optimal performance. Furthermore, Agar well diffusion method was used to check the antibacterial activities of the hydrogel using two bacterial pathogenic strains, i.e. Klebsiella pneumoniae (gram negative) and Enterococcus faecalis (gram positive). Results showed that after the inclusion of LNP into rGO hydrogel, there was a marked improvement in pollutant's uptake ability and compared to bare LNP and rGO, the composite hydrogel showed enhanced bactericidal effect. Overall, this approach is outstanding because of the synergy of functional properties of nano-lignin and rGO due to multi-interaction sites in the resulting hydrogel. The results presented herein support the application of rGO@LNP as innovative water filter material for scavenging broad spectrum pollutants and bactericidal properties.

Dosimetric Analysis of Rhizophora-based Phantom Material in Radiation Therapy Applications Using Monte Carlo GATE Simulation.

Purpose: This study aims to determine the percentage depth dose (PDD) of a phantom material made from soy-lignin bonded Rhizophora spp. particleboard coated with a gloss finish by using Monte Carlo Geant4 Application for Tomographic Emission (GATE) simulation. Materials and Methods: The particleboard was fabricated using a hot pressing technique at target density of 1.0 g·cm-3 and the elemental fraction was recorded for the simulation. The PDD was simulated in the GATE simulation using the linear accelerator Elekta Synergy model for the water phantom and Rhizophora phantom, and the results were compared with the experimental PDD performed by several studies. Beam flatness and beam symmetry were also measured in this study. Results: The simulated PDD for Rhizophora and water was in agreement with the experimental PDD of water with overall discrepancies of 0% to 8.7% at depth ranging from 1.0 to 15.0 cm. In the GATE simulation, all the points passed the clinical 3%/3 mm criterion in comparison with water, with the final percentage of 2.34% for Rhizophora phantom and 2.49% for the water phantom simulated in GATE. Both the symmetries are all within the range of an acceptable value of 2.0% according to the recommendation, with the beam symmetry of the water phantom and Rhizophora phantom at 0.58% and 0.28%, respectively. Conclusions: The findings of this study provide the necessary foundation to confidently use the phantom for radiotherapy purposes, especially in treatment planning.

Estimation of linear and mass attenuation coefficients of soy-lignin bonded Rhizophora spp. particleboard as a potential phantom material using caesium-137 and cobalt-60.

In this study, linear and mass attenuation coefficients of fabricated particleboards intended for use as phantom material were estimated using 137Cs and 60Co radiation sources. Particleboards made of Rhizophora spp. wood trunk bonded with soy flour and lignin were fabricated at a target density of 1.0 g cm-3, with and without gloss finish coating. Elemental composition of the particleboards was obtained by means of energy dispersive X-ray (EDX) spectroscopy. Experimental setups were simulated via the GATE Monte Carlo (MC) package, with particle histories of 1 × 106-1 × 107. Linear and mass attenuation coefficients obtained from measurements and GATE simulations were compared and discussed. The percentage differences between the measured and simulated linear and mass attenuation coefficients of the samples were reasonably small (2.05-4.88% for 137Cs and 3.24-5.38% for 60Co). It is shown that all the particleboards have the potential to be used as phantom materials as the attenuation coefficients measured were in good agreement with those of water (calculated with XCOM) and with those simulated with the GATE toolkit. The use of gloss finish coating also did not show any significant effect on the attenuation coefficient of the phantom material. Verification of experimental results via GATE simulations has been shown crucial in providing reliable data for energy transmission studies. Based on the results achieved in this study, it is concluded that the studied material-Rhizophora spp. wood trunk bonded with soy flour and lignin including gloss finish coating-can be used in radiation dosimetry studies.

Characterization and Ofloxacin Adsorption Studies of Chemically Modified Activated Carbon from Cassava Stem.

Cassava is a type of crop popular in Asian countries. It can be easily cultivated and grows to a mature plant in 9 months. Considering its availability, this work studied activated carbon based on cassava stem. Ofloxacin was chosen as the adsorbate, simulating the wastewater from the pharmaceutical industry. Cassava stem was ground into particles and heated to the activated state, 787 °C. The cassava-stem-activated carbon was further treated with the surface modifier, namely sodium hydroxide and zinc chloride, to study the improvement in ofloxacin adsorption. Prepared adsorbents were characterised using the SEM, FT-IR, XRD, DSC and TGA methods before being evaluated through batch adsorption, thermodynamic, and kinetic studies. The surface area analysis indicates that treatment of the activated carbon with NaOH and ZnCl2 increases the surface area due to the removal of organic content by the chemicals. Better ofloxacin adsorption of all activated carbon samples can be obtained with solutions at pH 8. An endothermic reaction was predicted, shown by higher ofloxacin adsorption at a higher temperature, supported by a positive value of ΔH° in the thermodynamic studies. The negative values of ΔG° revealed that adsorptions were spontaneous. The higher R2 values indicate that the adsorption process follows the pseudo-second-order equation of kinetic study. The maximum adsorption capacities are 42.37, 62.11, 62.89 and 58.82 mg/g for raw cassava stem (RC), cassava-stem-activated carbon (AC), NaOH-modified cassava-stem-activated carbon (NAC), and ZnCl2 modified cassava-stem-activated carbon (ZAC). The adsorption capacity is good compared to previous works by other researchers, making it a possible alternative material for the pharmaceutical industry's wastewater treatment.

In Vitro Evaluation of Antibacterial, Antioxidant, and Antidiabetic Activities and Glucose Uptake through 2-NBDG by Hep-2 Liver Cancer Cells Treated with Green Synthesized Silver Nanoparticles.

The alarming rise in diabetes owing to drug resistance necessitates the implementation of prompt countermeasures in the treatment module of diabetes. Due to their unique physicochemical features, silver nanoparticles may have potential applications in the medical and pharmaceutical industries. Silver nanoparticles (AgNPs) were synthesized from the culture filtrate of Salmonella enterica (ATCC-14028). UV-Vis spectrophotometry, FTIR, SEM, and energy dispersive X-rays were used in the characterization of the nanoparticles. Transmission electron microscopy (TEM) revealed that AgNPs are spherical and highly scattered and vary in size from 7.18 nm to 13.24 nm. AgNP stability and protein loss were confirmed by thermogravimetric analysis (TGA) at different temperatures. The AgNPs had excellent antibacterial activity and a strong synergistic effect against methicillin-resistant bacteria Staphylococcus aureus (MRSA) ATCC-4330 and Streptococcus epidermis (MRSE) ATCC-51625. The DPPH experiment revealed that the AgNPs had high antioxidant activity. The antidiabetic assay revealed that these AgNPs had an IC50 for alpha-amylase of 428.60 µg/ml and an IC50 for alpha-glucosidase of 562.02 µg/ml. Flow cytometry analysis of Hep-2 cells treated with AgNPs (40 µg/ml) revealed higher expression of 2-NBDG glucose absorption (uptake) compared to control metformin. These AgNPs have promising antidiabetic properties and could be used in pharmaceuticals and biomedical industries.

Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using Streptomyces sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against P. aeruginosa USM-AR2 and MRSA.

Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. Streptomyces sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs' antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against P. aeruginosa USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel's properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria P. aeruginosa and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.

Influence of Different Percentages of Binders on the Physico-Mechanical Properties of Rhizophora spp. Particleboard as Natural-Based Tissue-Equivalent Phantom for Radiation Dosimetry Applications.

Rhizophora spp. particleboard with the incorporation of lignin and soy flour as binders were fabricated and the influence of different percentages of lignin and soy flour (0%, 6% and 12%) on the physico-mechanical properties of the particleboard were studied. The samples were characterised by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), X-ray fluorescence (XRF) and internal bonding. The results stipulated that the addition of binders in the fabrication of the particleboard did not change the functional groups according to the FTIR spectrum. For XRD, addition of binders did not reveal any major transformation within the composites. SEM and EDX analyses for all percentages of binders added showed no apparent disparity; however, it is important to note that the incorporation of binders allows better bonding between the molecules. In XRF analysis, lower percentage of chlorine in the adhesive-bonded samples may be advantageous in maintaining the natural properties of the particleboard. In internal bonding, increased internal bond strength in samples with binders may indicate better structural integrity and physico-mechanical strength. In conclusion, the incorporation of lignin and soy flour as binders may potentially strengthen and fortify the particleboard, thus, can be a reliable phantom in radiation dosimetry applications.

Characterization of soy-lignin bonded Rhizophora spp. particleboard as substitute phantom material for radiation dosimetric studies - Investigation of CT number, mass attenuation coefficient and effective atomic number.

Experimental particleboards are made from Rhizophora spp. wood trunk with three different percentages of lignin and soy flour (0%, 6% and 12%) as adhesives. The objective was to investigate the equivalence of Rhizophora spp. particleboard as phantom material with human soft tissue using Computed Tomography (CT) number. The linear and mass attenuation coefficient of Rhizophora spp. particleboard at low energy range was also explored using X-ray Fluorescence (XRF) configuration technique. Further characterization of the particleboard was performed to determine the effective atomic number, Zeff using Energy Dispersive X-Ray (EDX) method. Adhesive-bonded Rhizophora spp. particleboard showed close similarities with water, based on the average CT numbers, electron density calibration curve and the analysis of CT density profile, compared to the binderless particleboard. The effective atomic number obtained from the study indicated that the attenuation properties of all the particleboards at different percentages of adhesives were almost similar to water. The mass attenuation coefficient calculated from XRF configuration technique showed good agreement with water from XCOM database, suggesting its potential as phantom material for radiation study.

Analysis using image segmentation for the elemental composition of activated carbon.

This article encompasses the method related to image segmentation of the Field Emission Scanning Electron Microscope (FESEM) images of Acacia Mangium Wood derived Activated Carbons under different conditions. Image segmentation using Hue-Saturation-Value (HSV) thresholding method was adapted to identify the different pattern composition in the grayscale images by varying the intensity Value (V) and keeping Hue (H) and Saturation (S) to zero, and each pattern was considered as one type of element that constituted the Activated Carbon. The algorithm was developed to compute the percentage of each pattern using non-zero pixels, and on the basis of different patterns, different elements having certain percentage of composition were recorded. Later, these results were compared with the Energy Dispersive X-ray Spectroscopy (EDS) to cross check the difference in percentage of each element present at the surface of the Activated Carbon. Part of this result is published in the article [1], "Comparison of surface properties of wood biomass Activated Carbons and their application against rhodamine B and methylene blue dye" Surfaces and Interfaces vol. 11 (2018) pp1-13.•The methods involved will be useful for characterization of Activated Carbon materials.•Image segmentation using HSV thresholding will inspire other researchers to apply similar concept on other materials.•Different patterns obtained for FESEM images using HSV thresholding was able to determine the presence of multiple elements present in the prepared Activated Carbon samples.

Scavenging of caffeine from aqueous medium through optimized H3PO4-activated Acacia mangium wood activated carbon: Statistical data of optimization.

The optimization data presented here are part of the study planned to remove the caffeine from aqueous solution through the large surface area optimized H3PO4-activated Acacia mangium wood activated carbon (OAMW-AC). The maximum adsorption capacity of the OAMW-AC for caffeine adsorption was achieved (30.3 mg/g) through optimized independent variables such as, OAMW-AC dosage (3.0 g/L), initial caffeine concentration (100 mg/L), contact time (60 min), and solution pH (7.7). The adsorption capacity of OAMW-AC was optimized with the help of rotatable central composite design of response surface methodology. Under the stated optimized conditions for maximum adsorption capacity, the removal efficiency was calculated to be 93%. The statistical significance of the data set was tested through the analysis of variance (ANOVA) study. Data confirmed the statistical model for caffeine adsorption was significant. The regression coefficient (R2) of curve fitting through the quadratic model was found to be 0.9832, and the adjusted regression coefficient was observed to be 0.9675.

Small temperature variations are a key regulator of reproductive growth and assimilate storage in oil palm (Elaeis guineensis).

Oil palm is an important crop for global vegetable oil production, and is widely grown in the humid tropical regions of Southeast Asia. Projected future climate change may well threaten palm oil production. However, oil palm plantations currently produce large amounts of unutilised biological waste. Oil palm stems - which comprise two-thirds of the waste - are especially relevant because they can contain high levels of non-structural carbohydrates (NSC) that can serve as feedstock for biorefineries. The NSC in stem are also considered a potent buffer to source-sink imbalances. In the present study, we monitored stem NSC levels and female reproductive growth. We then applied convergent cross mapping (CCM) to assess the causal relationship between the time-series. Mutual causal relationships between female reproductive growth and the stem NSC were detected, with the exception of a relationship between female reproductive organ growth and starch levels. The NSC levels were also influenced by long-term cumulative temperature, with the relationship showing a seven-month time lag. The dynamic between NSC levels and long-term cumulative rainfall showed a shorter time lag. The lower temperatures and higher cumulative rainfall observed from October to December identify this as a period with maximum stem NSC stocks.

Reinforced lignin-phenol-glyoxal (LPG) wood adhesives from coconut husk.

Lignin was extracted from coconut husk via alkaline pulping, either Kraft or soda. The isolated lignin samples were classified as hydroxy-benzaldehyde, vanillin, and syringaldehyde type according to Fourier-transform Infrared Spectroscopy, 1H and 13C Nuclear Magnetic Resonance (NMR) spectra. Soda lignin (SL) showed higher thermal stability and glass transition temperature (Tg) than Kraft lignin (KL) as proven by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively. The soda-lignin-phenol-glyoxal (SLPG) resins with the optimum percentage of lignin substitution at 30% showed improved solid content and gel time in comparison to 30% of Kraft-lignin-phenol-glyoxal (KLPG) and phenol-glyoxal (PG) resin. The good mechanical properties in SLPG is due to the higher amount of molecular weight as well as higher phenolic and G-type unit in lignin that improve the properties of 30% SLPG adhesive. Moreover, the addition of layered double hydroxides (LDH) as reinforced filler up to 15%-30% SLPG adhesive blend shows a great performance (especially mechanical properties) as compared to 30% SLPG adhesive alone.

Colorimetric Analysis of Glucose Oxidase-Magnetic Cellulose Nanocrystals (CNCs) for Glucose Detection.

Glucose oxidase (EC 1.1.3.4) sensors that have been developed and widely used for glucose monitoring have generally relied on electrochemical principle. In this study, the potential use of colorimetric method for glucose detection utilizing glucose oxidase-magnetic cellulose nanocrystals (CNCs) is explored. Magnetic cellulose nanocrystals (magnetic CNCs) were fabricated using iron oxide nanoparticles (IONPs) and cellulose nanocrystals (CNCs) via electrostatic self-assembly technique. Glucose oxidase was successfully immobilized on magnetic CNCs using carbodiimide-coupling reaction. About 33% of GOx was successfully attached on magnetic CNCs, and the affinity of GOx-magnetic CNCs to glucose molecules was slightly higher than free enzymes. Furthermore, immobilization does not affect the specificity of GOx-magnetic CNCs towards glucose and can detect glucose from 0.25 mM to 2.5 mM. Apart from that, GOx-magnetic CNCs stored at 4 °C for 4 weeks retained 70% of its initial activity and can be recycled for at least ten consecutive cycles.

Bioengineered silver nanoparticles capped with bovine serum albumin and its anticancer and apoptotic activity against breast, bone and intestinal colon cancer cell lines.

The aim of the current study was to biosynthesize the silver nanoparticles (AgNPs) from the bacterial strain of Bacillus cereus (ATCC 14579) extracellularly. When bacterial extract was challenged with 1 mM silver nitrate (AgNO3) the color of the extract changed into brown confirms the formation of nanoparticles. These nanoparticles were capped with bovine serum albumin (BSA). UV- visible spectroscopy showed the absorption peak at 420 nm indicates the formation of AgNPs. Fourier Infra -red (FTIR) attenuated total reflection (ATR) spectroscopy showed amide and amine group associated with AgNPs that stabilizes the nanoparticles. Energy dispersive x-ray spectroscopy (EDX) showed a strong peak of silver confirms the presence of silver. Thermo gravimetric analysis (TGA) analysis was used to determine the protein degradation showed less protein degradation at higher temperature confirms the stability of nanoparticles. Transmission electron microscopy (TEM) showed the AgNPs are well dispersed and spherical, and 5.37 nm to 17.19 whereas albumin coated nanoparticles are size ranges from 11.26 nm to 23.85 nm. The anticancer effect of capped AgNPs (cAgNPs) showed the IC50 value against breast cancer MCF-7 at 80 µg/mL, intestinal colon cancer HCT- 116 60 µg/mL, and bone cancer osteosarcoma MG-63 cell line80 µg/mL while against normal fibroblast cells 3T3 cells showed the IC50 value at 140 µg/mL. Lactate dehydrogenase assay (LDH) showed higher toxicity on MCF-7, HCT-116, and MG-63 cells. The apoptotic study clearly showed the blebbing of membrane, chromatin condensation due to the production of reactive oxygen species (ROS) by ethidium bromide and acridine orange dual staining method. The DNA analysis showed the complete fragmentation of the DNA of treated cells when compared with control cells.

Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.

The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide. Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ±â€¯39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps. We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions. The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1. Moreover, thermodynamic study of the system revealed spontaneous and endothermic nature of the sorption process. Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite. This could essentially be attributed to synergistic effects of size particularity (nano-effect) and incorporated functionalities due to lignin nanoparticles. Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%. Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.

Biodegradation of fibrillated oil palm trunk fiber by a novel thermophilic, anaerobic, xylanolytic bacterium Caldicoprobacter sp. CL-2 isolated from compost.

Oil palm trunk (OPT) is one of the most promising lignocellulosic bioresources. To develop effective biodegradation, thermophilic, anaerobic microorganisms were screened from bovine manure compost using fibrillated OPT (f-OPT) pretreated by wet disk milling as the substrate. One thermophilic, anaerobic bacterium, strain CL-2, whose 16S rDNA gene has 98.6% sequence identity with that of Caldicoprobacter faecale DSM 20678T, exhibited high degradation activity (32.7% reduction in total dry solids of f-OPT). Strain CL-2 did not use cellulose as a carbon source, but used hemicelluloses such as xylan, arabinoxylan, starch and pectin at 70 °C. Phylogenetic and morphologic analyses and the polysaccharide use suggest that CL-2 may be classified as a novel species of Caldicoprobacter, named Caldicoprobacter sp. CL-2. To characterize enzymatic activities of CL-2, extracellular enzymes were prepared from culture broth using beechwood xylan as the carbon source. The extracellular enzymes showed high xylanase activity, but low cellulase activity, suggesting that f-OPT degradation may depend on xylanase activity. To understand the xylanase system of CL-2, a major xylanase was cloned and characterized. The xylanase (CalXyn11A) had a modular structure consisting of a glycoside hydrolase (GH) family-11 domain and a family 36 carbohydrate-binding module. CalXyn11A did not show f-OPT degradation activity, but a strong synergistic effect was observed when CalXyn11A was added to the extracellular enzyme preparation. These results indicate that, rather than working alone, CalXyn11A has an important role in enhancing total lignocellulose degradation activity by cooperation with other GHs.