[Study on preparation and quality standard of Xueshuantong Drop Pills].

OBJECTIVE: To optimize the best technical parameters and establish the quality standard for Xueshuantong Drop Pills. METHODS: Using moulding probability of drop pill as the evaluation to choose the best dropping condition. Using parallel experimentation for the choice of proportion of drug and matrix, and refrigerant; HPLC method was employed on ODS column which was used with a mobile phase of acetontrile-water, 0 min 20% A, 14 min 40% A, 15 min 60% A, 16 min 20% A. The wavelength of detexter was set at 203 nm. Drug-PEG4000 (1:2) was melt at 80 degrees C. Liquid-paraffin was refrigerated with 5 degrees C. Internal and external diameter of buretteare equal to 2.3 mm and 4. 8 mm. The linear ranges of notoginsenoside R1 ginsenoside, Rg1 and Rb1 were 0.51-4.08 +/- microg/mL, 3.75-30.00 microg/mL, 3.75-30.00 microg/mL, respectively. Their coefficients of determination (r) were all 0.9999. CONCLUSION: The moulding technics of drop pills have high finished product rate and good quality.

[Preparation of new lipid-hydroxyapatite-DNA complex and gene transfection reseach in eukaryotic cell].

This work was directed at obtaining a better gene carrier to improve the effects of gene delivery. Neutral liposomes made from cholesterol, lecithin and DOPE by reverse evaporation technique were used for encapsulating DNA-HAP complex which was made from DNA and optimized HAP. The sizes of complexes and the efficiency of encapsulation were detected. The efficiency of transfection into Hela cells was shown by observation of X-gal staining and measurement of transfection efficience. The average size of complexes was 643nm, the average encapsulating efficiency of DNA in microspheres reached 11.67%. These Lipid-Hydroxyapatite-DNA complex (LHD) could be transfected into mammalian cells. The Lipid-Hydroxyapatite-DNA complex prepared by reverse evaporation technique could be applied availably in DNA delivery system, and it gave another thinking to increase the gene transfection of non- viral genetic vector.

[Human parasitology teaching in the 21st century].

Multimedia techniques were applied in parasitological teaching and experimental practices. In order to strengthen the practical ability of the undergraduate students, reform was conducted including a prioritization of the teaching content, use of series micro-slides and video show, etc.