Epidemiological Characteristics and Genotypic Features of Rotavirus and Norovirus in Jining City, 2021-2022.

Diarrhea, often caused by viruses like rotavirus (RV) and norovirus (NV), is a global health concern. This study focuses on RV and NV in Jining City from 2021 to 2022. Between 2021 and 2022, a total of 1052 diarrhea samples were collected. Real-Time Quantitative Fluorescent Reverse Transcriptase-PCR was used to detect RV-A, NV GI, and NV GII. For RV-A-positive samples, VP7 and VP4 genes were sequenced for genotype analysis, followed by the construction of evolutionary trees. Likewise, for NV-GII-positive samples, VP1 and RdRp genes were sequenced for genotypic analysis, and evolutionary trees were subsequently constructed. Between 2021 and 2022, Jining City showed varying detection ratios: RV-A alone (excluding co-infection of RV-A and NV GII) at 7.03%, NV GI at 0.10%, NV GII alone (excluding co-infection of RV-A and NV GII) at 5.42%, and co-infection of RV-A and NV GII at 1.14%. The highest RV-A ratios were shown in children ≤1 year and 2-5 years. Jining, Jinxiang County, and Liangshan County had notably high RV-A ratios at 24.37% (excluding co-infection of RV-A and NV GII) and 18.33% (excluding co-infection of RV-A and NV GII), respectively. Jining, Qufu, and Weishan had no RV-A positives. Weishan showed the highest NV GII ratios at 35.48% (excluding co-infection of RV-A and NV GII). Genotype analysis showed that, in 2021, G9P[8] and G2P[4] were dominant at 94.44% and 5.56%, respectively. In 2022, G8P[8], G9P[8], and G1P[8] were prominent at 75.86%, 13.79%, and 10.35%, respectively. In 2021, GII.3[P12], GII.4[P16], and GII.4[P31] constituted 71.42%, 14.29%, and 14.29%, respectively. In 2022, GII.3[P12] and GII.4[P16] accounted for 55.00% and 45.00%, respectively. RV-A and NV showed varying patterns for different time frames, age groups, and regions within Jining. Genotypic shifts were also observed in prevalent RV-A and NV GII strains in Jining City from 2021 to 2022. Ongoing monitoring of RV-A and NV is recommended for effective prevention and control.

Research on the characteristics of EV interior sound quality and its dynamic active control system design and development un-der accelerated driving conditions.

In order to improve the interior sound quality of Electric Vehicles (EV), solve the problem of low sense of power and comfort of the interior sound as well as the large electromagnetic excitation order noise of motor and the sharp interior sound, this article designs a dynamic active sound control system for EV under accelerated driving conditions. Firstly, by comparing and analyzing the sound spectrum characteristics of fuel vehicle (FV) and EV during acceleration, a short-time Fourier transform (STFT) is adopted to extract and synthesize the engine sound. Secondly, the influence of the engine order composition and the energy distribution in the frequency domain on the sound quality of the vehicle is analyzed, and an active control system for sound quality is proposed. And the software and hardware development of the active control sound system is completed. Finally, through real-vehicle testing and verification, the sense of comfort and power of the EV interior sound has been greatly improved during acceleration, and the total value of interior sound can meet the requirement. The sound pressure level and loudness of interior sound have been increased, and the sharpness of the sound inside the vehicle has been improved, with a maximum reduction of 1.0acum.

Comprehensive genomic analysis of the SARS-CoV-2 Omicron variant BA.2.76 in Jining City, China, 2022.

OBJECTIVE: This study aims to analyze the molecular characteristics of the novel coronavirus (SARS-CoV-2) Omicron variant BA.2.76 in Jining City, China. METHODS: Whole-genome sequencing was performed on 87 cases of SARS-CoV-2 infection. Evolutionary trees were constructed using bioinformatics software to analyze sequence homology, variant sites, N-glycosylation sites, and phosphorylation sites. RESULTS: All 87 SARS-CoV-2 whole-genome sequences were classified under the evolutionary branch of the Omicron variant BA.2.76. Their similarity to the reference strain Wuhan-Hu-1 ranged from 99.72 to 99.74%. In comparison to the reference strain Wuhan-Hu-1, the 87 sequences exhibited 77-84 nucleotide differences and 27 nucleotide deletions. A total of 69 amino acid variant sites, 9 amino acid deletions, and 1 stop codon mutation were identified across 18 proteins. Among them, the spike (S) protein exhibited the highest number of variant sites, and the ORF8 protein showed a Q27 stop mutation. Multiple proteins displayed variations in glycosylation and phosphorylation sites. CONCLUSION: SARS-CoV-2 continues to evolve, giving rise to new strains with enhanced transmission, stronger immune evasion capabilities, and reduced pathogenicity. The application of high-throughput sequencing technologies in the epidemic prevention and control of COVID-19 provides crucial insights into the evolutionary and variant characteristics of the virus at the genomic level, thereby holding significant implications for the prevention and control of the COVID-19 pandemic.

Neuroprotective effects of galectin­1 on cerebral ischemia/reperfusion injury by regulating oxidative stress.

Oxidative stress contributes to the pathology of cerebral ischemia/reperfusion (I/R) injury. Galectin-1 has shown an anti-oxidative stress effect. The present study investigated whether this anti-oxidative stress effect can account for the neuroprotective actions of galectin-1 induced by cerebral I/R injury. A cerebral I/R injury model was created in C57Bl/6 mice by transient occlusion of the middle cerebral artery, after which the mice were treated with galectin-1 for 3 days. Infarct volumes were measured. A rotarod test and neurological deficit score assessment was performed to evaluate the neurological deficits. Oxidative stress was evaluated by measuring the levels of reactive oxygen species (ROS) and lipid peroxidation malondialdehyde (MDA), while the anti-oxidative stress status was assessed by measuring molecules such as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidation enzyme (GSH-Px) in the ischemic cerebral hemisphere of mice. The inflammatory cytokines, including Interleukin 1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α) were measured, and the expression of microglia was evaluated by immunohistochemistry in the ischemic cerebral hemisphere of mice. Galectin-1 treatment ameliorated neurological deficits and reduced infarct volumes in the mice model with cerebral I/R injury. Moreover, it was demonstrated that galectin-1 can significantly alleviate cerebral I/R injury in the ischemic cerebral hemisphere by decreasing the production of ROS and MDA, but increasing the production of CAT, SOD and GSH-Px. Galectin-1 treatment decreased microglia expression, and IL-1, IL-6 and TNF-α levels in the ischemic cerebral hemisphere of mice. Galectin-1 could improve the outcome of cerebral I/R injury by alleviating oxidative stress. Moreover, the neuroprotective effect of galectin-1 in cerebral ischemia could be related to its anti-oxidative stress effect.

First Report of Alternaria alternata Causing Leaf Spot on Coffea arabica in China.

Yunnan Province is the major region for coffee (Coffea arabica) cultivation in China, contributing to over 98% of the national yield and total production value (Ma et al. 2022). In May 2023, brown spot symptoms were observed only on the leaves of coffee plants in a field located in Baoshan City (98°52'37.988400"E, 24°58'17.673600"N), Yunnan Province. Notably, brown and irregularly shaped spots initially started on the leaf bases. The spots enlarged and developed concentric rings with dark brown margins, which are often surrounded by yellow halos. Finally, the necrotic spots spread across the entire leaf and caused the leaf to curl and fall off. The incidence of the disease was approximately 3% of the coffee plants (n = 600). The symptomatic leaves collected from 10 plants were sectioned (5 × 5 mm), subjected to surface sterilization with 70% ethanol for 40 s, rinsed with sterile distilled water, air-dried, and transferred to potato dextrose agar (PDA). Fungi with grayish-white, cotton-like aerial mycelia grew after 7 days at 28°C. The older mycelia of these isolates displayed dark gray pigmentation. Single conidia were cultivated on PDA, and 15 morphologically similar monosporic isolates were ultimately obtained. Microscopic observation revealed that these isolates produced branched, septate, transparent and amber mycelium. Brown, elliptical or pear-shaped conidia with 2 to 4 transversal septa and 0 to 3 longitudinal septa, measuring 9.6 to 33.3 long × 6.0 to 15.0 µm wide (n = 30), were observed on potato carrot agar (PCA). Molecular identification of multiple genes, such as ITS (Schoch et al. 2012), RPB2 (O'Donnell et al. 2010) and GAPDH (Berbee et al. 1999), indicated consistent 100% identity among these isolates. Sequences of the representative isolates CFSY1-CFSY5 were deposited in GenBank (acc. nos.: OR351112, PP188577, PP188578, PP294863, PP294864, OR509742, PP215341-PP215344, OR509740 and PP239378-PP239381), revealing 98.35% - 100% homology with distinct Alternaria alternata strains previously deposited in GenBank (acc. nos.: PP110780, MN649031 and OR485338). The multigene phylogenetic analysis positioned isolates CFSY1-CFSY5 within a distinct cluster, alongside diverse A. alternata isolates. Based on morphological and molecular characterizations, the pathogen was identified as A. alternata. To verify its pathogenicity, a conidial suspension (1×106 conidia/mL) of isolate CFSY1 was sprayed on six leaves of three healthy one-year-old C. arabica seedlings. Subsequently, the inoculated seedlings were covered with plastic bags and placed in a growth chamber under controlled conditions (a 14 h daylight period and a 10 h dark period at 28°C). The experiment was repeated three times. After 20 days, typical brown spot symptoms analogous to those originally observed in the field appeared on the leaves in all inoculated plants. Reisolation, morphology identification and DNA sequencing substantiated Koch's postulates. In contrast, control plants treated with sterilized water remained asymptomatic, and no pathogen was reisolated from them. Significantly, A. alternata has been previously reported as the causal agent for leaf spot disease in a diverse variety of woody plant species in China, including Prunus avium (Ahmad et al. 2020), Magnolia grandiflora (Liu et al. 2019) and citrus (Wang et al. 2010). This study represents the first report of brown leaf spot caused by A. alternata specifically on C. arabica in China, enriching the contents of fungal pathogens under Chinese coffee cultivation conditions.

The antifungal metabolites from coculture of Aspergillus fumigatus and Alternaria alternata associated with Coffea arabica.

One new cyclohexenone derivative, asperfumtone A (1) along with six known compounds were obtained from the coculture of Aspergillus fumigatus and Alternaria alternata associated with Coffea arabica. The configuration of 2 was first reported in the research. The structures were determined by extensive spectroscopic analyses, and ECD calculation. Compounds 3, 4 and 7 showed significant antifungal activities against coffee phytopathogens A. alternata and Fusarium incarnatum with MICs of 1 µg/mL. Compounds 1 and 2 showed weak antifungal activities against A. alternata and F. incarnatum with MICs of 32-64 µg/mL.

Synthesis of the 5-6-7 Tricyclic Core of Daphnicyclidin-Type Alkaloids via a Tiffeneau-Demjanov Ring Enlargement Strategy.

An expedient construction of the 5-6-7 tricyclic core of daphnicyclidin-type alkaloids is described. The synthetically challenging cycloheptanone C ring was constructed via a Tiffeneau-Demjanov ring enlargement reaction from a 5-6-6 tricyclic precursor commonly found in calyciphylline A-type alkaloids. Other key transformations included Davis oxidation, 1,2-addition, oxidation, and dehydration to elaborate the essential cyclcohept-2-enone motif.

Differences in microbial community composition and factors affecting different particulate matter during autumn in three cities of Xinjiang, China.

Environmental pollution has become an issue of increasing concern in China, owing to the country's rapid economic development. Atmospheric particulate matter (PM) is known to be an important parameter in air quality monitoring; further, bioaerosol forms a crucial component of PM. As the climatic environments in the north and south of Xinjiang, China, are significantly different, here, atmospheric PM samples collected from three cities, Shihezi, Yining, and Tumushuk, located in different directions, were analysed for a better understanding of the spatial distribution patterns of microbial community composition of Xinjiang. The16s rDNA and 18 s rDNA were used to locate bacteria and fungi in PM2.5, PM10, and total suspended particulate matter (TSP) at the species level and genus level, and the microbial communities with the top 15 abundances were selected for analysis. The reports indicate that the most abundant group in Shihezi and Yining was Cenchrus_americanus, which belongs to Proteobacteria. The remaining 14 dominant species had their own distribution pattern in each city. The most dominant strain in Tumushuk was Bacillus_taeanensis, but this strain was not detected in Yining and Shihezi. Similarly, the most predominant fungus in Tumushuk (Microdorylaimus_miser under Myriophyllum) was not detected in the other two cities. The analysis of the effect of environmental impact factors on bacteria and fungi revealed that the impact factors such as temperature, humidity, and wind speed had a greater effect on microorganisms, while O3 had a negative correlation with most microorganisms, owing to its toxicity. Overall, the results of this study show that short-range transported air masses have a greater impact on local pollutants and microorganisms.

Application of traditional Chinese medicine in film drug delivery system.

Film drug delivery systems have the advantages of precise administration, simple process and easy portability, compared with other traditional drug delivery systems such as tablets, capsules, syrups, ointments, etc. The traditional Chinese medicine (TCM) are normally developed in four categories of film agent like patch film, coating, spray film and gel film, which are applied to the treatment of oral ulcers, chronic diseases of lower limbs, burns, scalds, gynecological disease and body care. So the TCM film has great research value and prominent market prospect. In this review, we summarized the research progress of the material composition, pharmaceutical production, clinical application and pharmacology mechanism of various TCM film agents. It may provide a comprehensive reference for further development and utilization of TCM film agents.

Ten-Step Total Synthesis of (-)-Andranginine.

The total synthesis of the indole alkaloid (-)-andranginine has been achieved in 10 steps. Key reactions of the synthesis include a nucleophilic addition of acetylenyl anion to chiral N-sulfinyl imine, an intramolecular N-alkylation reaction to close the C ring, and a dienyne metathesis cascade reaction to construct the DE rings. Meanwhile, 16-epi-(-)-andranginine was also obtained with the developed strategy.

Effect of Parameters of Pool Geometry on Flow Structure in Nature-like Fishway.

Many uncertainties such as variable irregular structure and complex flow conditions bring difficulties to the design of a nature-like fishway. This study defines the main factors and parameters affecting flow conditions such as permeability ratio, offset ratio, bottom slope and pool length to simplify and generalize the irregular geometry of the nature-like fishway. According to the engineering requirements of the Mopiling nature-like fishway, the effect of the above parameters of pool geometry on the flow structure is investigated through a 3D turbulent numerical simulation, and the parameter thresholds are summarized according to the optimization of the flow conditions. The results show that under the same conditions, the maximum velocity of the control section increases with the increase in permeability ratio, bottom slope and pool length, and the offset ratio has limited effect on the maximum velocity of the control section. It is recommended that when the bottom slope is 1/250 and the pool length is 10 m, the permeability ratio should not be greater than 0.30 and the offset ratio should be located between 0.15 and 0.60. When the bottom slope is adjusted to 1/200, it is recommended to control the permeability ratio below 0.20, the offset ratio between 0.30 and 0.60, and the pool length can be adjusted to 8 m. Within the above threshold range of the design parameters, the maximum velocity in the fishway can be basically controlled at about 1.0 m/s. The mainstream in the pool is clear and the flow pattern is good, which can basically satisfy the requirements of fish passing. The relevant design parameters and optimization strategies can provide reference for similar projects.

Characteristics of Environmentally Persistent Free Radicals in PM2.5 and the Influence of Air Pollutants in Shihezi, Northwestern China.

Environmentally persistent free radicals (EPFRs) are a kind of hazardous substance that exist stably in the atmosphere for a long time. EPFRs combined with fine particulate matter (PM2.5) can enter the human respiratory tract through respiration, causing oxidative stress and DNA damage, and they are also closely related to lung cancer. In this study, the inhalation risk for EPFRs in PM2.5 and factors influencing this risk were assessed using the equivalent number of cigarette tar EPFRs. The daily inhalation exposure for EPFRs in PM2.5 was estimated to be equivalent to 0.66-8.40 cigarette tar EPFRs per day. The concentration level and species characteristics were investigated using electron paramagnetic resonance spectroscopy. The concentration of EPFRs in the study ranged from 1.353-4.653 × 1013 spins/g, and the types of EPFRs were mainly oxygen- or carbon-centered semiquinone-type radicals. Our study showed that there is a strong correlation between the concentrations of EPFRs and conventional pollutants, except for sulfur dioxide. The major factors influencing EPFR concentration in the atmosphere were temperature and wind speed; the higher the temperature and wind speed, the lower the concentration of EPFRs. The findings of this study provide an important basis for further research on the formation mechanism and health effects of EPFRs.

Laboratory Studies on the Rheotaxis of Fish under Different Attraction Flow Conditions.

The damming of the river changes the structure of the original river ecosystem, and although fish passage plays an important role in maintaining the connectivity of the river ecosystem, the fish have difficulty finding the fish passage entrance during the upstream process. This paper studied the rheotaxis of fish under three different water flow conditions experimentally through recirculating water tanks. To better understand the response of Crucian carp (Carassius auratus) to water flow stimulation, the representative swimming trajectory, sensing success rate, attraction success rate, reaction time, and attraction time of the fish were analyzed by using a video monitoring system. The experimental results showed that fish responded differently to single-peak and lateral bimodal outflow conditions: (1) the single-peak outflow condition had a much better attraction effect than the lateral bimodal outflow condition, both in terms of sensing success rate and attraction success rate; (2) the fish swam mainly in the middle area of the lateral bimodal outflow condition, while the fish swam more evenly in the single-peak outflow condition. Therefore, setting the attraction current at the right time and near the entrance of the fish passage may help to improve the effect of fish attraction.

Collective Total Syntheses of Five Lycopodium Alkaloids.

It is reported herein that by exploiting the commonly shared bicyclic decahydroquinoline motif, a gold-catalyzed enamide-alkyne cycloisomerization reaction is developed to access tricyclic cores in a simple way. These tricyclic cores further serve as an advanced platform for the divergent enantioselective collective total syntheses of five Lycopodium alkaloids, belonging to three different structural types, in a concise and protecting-group-free fashion. The key transformations in the second phase include: 1) a transannular reductive Heck cyclization for installation of the azepane ring in fawcettidine, fawcettimine, and lycoposerramine Q; 2) a domino Mukaiyama hydration/Grob fragmentation process for construction of the ten-membered lactam system in phlegmariurine B; 3) a Fukuyama one-pot protocol for the construction of the 2-pyridone motif in lycoposerramine R. The newly developed strategy is expected to pave the way for the synthesis of other structurally related Lycopodium alkaloids.

Microorganism community composition analysis coupling with 15N tracer experiments reveals the nitrification rate and N2O emissions in low pH soils in Southern China.

Nitrification in agricultural soil is an important process for food production. In acidic soil, nitrification is however also considered to be a major source of N2O production. The nitrification rate largely depends on the community composition of ammonia-oxidizing organisms. To obtain a view of the nitrification rates and N2O emission situations in low pH soils in Southern China and understand their relations with the microbial community composition, here we conducted 15N tracer experiments and microorganism community composition analysis using four acidic agricultural soil samples collected in Southern China. A single dominant community (relative abundance >68%) of the ammonia-oxidizing bacteria and ammonia-oxidizing archaea was observed in the soils with pH = 4.81-6.02. A low amount of NO 3 - was produced from the nitrification in the strongly acidic soil (pH = 4.03), and the calculated nitrification rate in this soil was significantly lower than those of other soils with pH = 4.81-6.02. High N2O emissions but low 15N-N2O emissions were observed in the soil with pH = 4.03. Our results suggest that, under aerobic conditions, soil pH is an important factor affecting nitrification through modifying the microorganism composition.

QTL Identification for Stem Fiber, Strength and Rot Resistance in a DH Population from an Alien Introgression of Brassica napus.

Stem fiber, stem strength and stem-rot resistance are important agronomic traits in Brassica napus. To understand the molecular mechanism that controls the stem-related traits, we investigated the stem lignin (ADL), cellulose (Cel), hemicellulose (Hem) content, S/G monolignol ratio (SG), stem breaking force (BF), breaking strength (F) and Sclerotinia sclerotiorum resistance (SSR). Each trait was significantly positively or negatively correlated with more than three of the other six traits. QTL mapping for ADL, Cel, Hem, SG, BF, F and SSR were performed using a doubled haploid population derived from an intertribal B. napus introgression line 'Y689' crossed with B. napus cv. 'Westar'. A total of 67 additive QTL were identified and integrated into 55 consensus QTL by meta-analysis. Among the 55 consensus QTL, 23 (41.8%) QTL were co-located and were integrated into 11 unique QTL. The QTL by environment (Q × E) interactions were analyzed and 22 combined QTL were identified. In addition, candidate genes within the QTL intervals were proposed based on the known function of Arabidopsis orthologs. These results provided valuable information for improving lodging resistance, S. sclerotiorum resistance and mechanized harvesting of B. napus.

Proton or Carbene Transfer? On the Dark and Light Reaction of Diazoalkanes with Alcohols.

The formal alkylation reaction of OH groups with diazoalkanes under catalyst-free reaction conditions finds broad application in organic synthesis. However, even today, this reaction is mainly limited to the use of diazomethane as reaction partner. In this combined experimental and theoretical study, we aim at a fundamental understanding of the reaction of diazoalkanes with alcohols to make this transformation amenable to a generalized approach towards formal alkylation reactions of alcohols with diazoalkanes. Experimental and theoretical studies suggest a direct proton transfer only in exceptional cases. In a more general setting, such O-H functionalization proceed both under dark and photochemical conditions via a key hydrogen-bonded singlet carbene intermediate that undergoes a protonation-addition mechanism. We conclude with applications of this approach in O-H functionalization reactions of alcohols, including simple fluorinated, halogenated and aliphatic alcohols and showcase functional-group tolerance of this method in the reaction of biologically active and pharmaceutically relevant alcohols.

Morphology and distribution of antennal sensilla in adults of Xylotrechus quadripes.

Xylotrechus quadripes (Chevrolat) is the serious woodborer pest of arabica coffee (Coffea arabica L.). To further elucidate the behavior mechanism of the insect based on chemical odorant and to advance effective trapping methods, the typology, distribution, and abundance of antennal sensilla were investigated meticulously in both sexes of X. quadripes by scanning electron microscopy. The filiform antennae of both sexes are composed of 11 segments, namely the scape, pedicel, and nine flagellomeres (f1-9). Ten types (14 subtypes) of sensilla were identified morphologically: sensilla chaetica (three subtypes, Ch.1-3), sensilla basiconica (three subtypes, Ba.1-3), Böhm bristles (Bb), sensilla dentiform (De), sensilla trichodea (Tr), sensilla auricillica (Au), sensilla campaniformia (Ca), grooved peg sensilla (Gp), cuticular pores (Cp), and a newly observed sensillum, named sensilla cone (Cone). The sensilla were mainly distributed in flagellomeres, and the types and amounts increased in frequency from scape to the ninth flagellomere. The numbers of sensilla were the highest on the antennal dorsal side, while the lowest on the ventral side. The types of sensilla were the most abundant on the lateral side, and two sensilla basiconica (Ba.1-2) were found exclusively. The average number of Tr, Ba, and Au on the antenna of the males was significantly greater than females, while the Gp and Cp on the antenna of the females were significantly greater than males. Ca was exclusively occurred on the male antennae but was absent in females. This study discusses the putative functions of the antennal sensilla in adults of X. quadripes based on their characteristics in related species' sensilla, and these results provide an important foundation to clarify the ecological adaption, olfactory recognition mechanism, and to develop the chemical ecology control of X. quadripes.

Lake ecosystem health assessment using a novel hybrid decision-making framework in the Nam Co, Qinghai-Tibet Plateau.

Lake health assessment (LHA), a powerful tool for lake ecological protection, provides the foundation for sustainable water environment management. However, existing methods have not yet considered the effects of fuzziness and randomness on LHA. In addition, most of the current studies on LHA focus on the plain areas, lack of quantitative studies in mountain areas, such as the Qinghai-Tibet Plateau. The Pythagorean fuzzy cloud (PFC) integration algorithm drawing on the advantages of Pythagorean fuzzy sets (PFS) and cloud model was proposed. A novel hybrid decision-making framework combining PFC integration algorithm and TOPSIS model was developed to determine the lake health levels with fuzziness and randomness. An indicator system incorporating ecosystem integrity (physical habitat, water quantity and quality, aquatic life) and non-ecological performance (social services) was established. To comprehensively investigate the lake health level in the Qinghai-Tibet Plateau, the Nam Co was selected as study area. Our results confirm that the developed framework in this study can overcome the shortcomings of existing methods and provide a more effective approach for LHA with fuzziness and randomness. In Nam Co, the non-ecological performance was significantly better than the ecosystem integrity. Health levels exhibited a remarkable spatial variation influenced by tourism and grazing, with decreasing health status from the northwestern to southeastern Nam Co. Approximately 85% of the sampling sites were at excellent or healthy levels, 15% were subhealthy, and no sampling sites were unhealthy and sick. Our results highlight that tourism has affected health levels at Nam Co, and effective measures are needed to minimize the impact in ecological fragile areas.

Effect of Adding L-carnitine to High-Fat/Low-Protein Diets of Common Carp (Cyprinus carpio) and the Mechanism of Regulation of Fat and Protein Metabolism.

L-carnitine is a low molecular weight amino acid that plays an essential role in the oxidation of long-chain fatty acids. The regulatory effects and molecular mechanisms of L-carnitine on fat and protein metabolism in common carp (Cyprinus carpio) were investigated in this study. Common carp (n = 270) were randomly divided into three groups and fed either (1) common carp diet, (2) high-fat/low-protein diet, or (3) L-carnitine-high-fat/low-protein diet. Growth performance, plasma biochemistry, muscle composition, and ammonia excretion rate were all examined after 8 weeks. Additionally, each group's hepatopancreas was subjected to transcriptome analysis. The results showed that decreasing the feed protein/fat ratio resulted in a considerable increase in feed conversion ratio and a significant decrease in common carp-specific growth rate to 1.19 ± 0.02 (P < 0.05). Similarly, total plasma cholesterol sharply increased to 10.15 ± 2.07, while plasma urea nitrogen, muscle protein, and ammonia excretion levels dropped (P < 0.05). After adding L-carnitine to the high-fat/low-protein diet, it was found that the specific growth rate and protein content of the dorsal muscle increased significantly (P < 0.05). In contrast, the plasma total cholesterol and ammonia excretion rate decreased considerably at most time points after feeding (P < 0.05). The expression of genes in the hepatopancreas differed substantially between the different groups. Through GO analysis, it was demonstrated that L-carnitine increased the ability of fat decomposition by up-regulating the expression of cpt1 in the hepatopancreas and decreased the expression of fasn and elovl6 to reduce the production and extension of lipids. Simultaneously, mtor was more abundant in the hepatopancreas, implying that L-carnitine can increase protein synthesis. According to the findings, adding L-carnitine to high-fat/low-protein diets can stimulate growth by enhancing lipolysis and protein synthesis.