A Bio-Liposome Activating Natural Killer Cell by Illuminating Tumor Homogenization Antigen Properties.

Natural killer (NK) cell therapies, primarily based on chimeric antigen receptor NK cells (CAR-NK), have been developed and applied clinically for therapeutic treatment of patients with mid-to-late-stage tumors. However, NK cell therapy has limited efficacy due to insufficient antigen expression on the tumor cell surface. Here, a universal "illuminate tumor homogenization antigen properties" (ITHAP) strategy to achieve stable and controlled antigen expression on the surface of tumor cells using nanomedicine, thus significantly enhancing the immune recognizability of tumor cells, is described. The ITHAP strategy is used to generate bio-liposomes (Pt@PL-IgG) composed of intermingled platelet membranes and liposomes with NK-activatable target antigen (IgG antibodies) and cisplatin pre-drug. It is demonstrated that Pt@PL-IgG successfully targets tumor cells using the autonomous drive of platelet membranes and achieves IgG implantation on tumor cells by utilizing membrane fusion properties. Moreover, it is shown that the Pt-DNA complex combined with NK cell-induced pyroptosis causes substantial interferon (IFN) secretion, thus providing a synthase-stimulator of interferon genes (STING)-IFN-mediated positive immune microenvironment to further potentiate NK therapy. These results show that anchoring cancer cells with NK-activatable target antigens is a promising translational strategy for addressing therapeutic challenges in tumor heterogeneity.

Construction and Analysis of Performance Evaluation Index System for Chinese Small and Medium-Sized Enterprises Based on Fuzzy Hierarchical Analysis Model.

It is of great theoretical and practical significance to introduce the supply chain concept into micro and small manufacturing enterprises and to build a cost management evaluation model for micro and small manufacturing enterprises based on the supply chain to improve the cost management of micro and small manufacturing enterprises. To this end, based on combing the relevant literature on supply chain and cost management evaluation at home and abroad, this paper analyzes the characteristics of cost management of micro and small manufacturing enterprises because of the problems of cost management of micro and small manufacturing enterprises, adopts the gray fuzzy hierarchical analysis method to assign and evaluate, which takes into account the nonindependence among the elements of each level and the elements of the same level, and also considers the characteristics of grayness and fuzziness of qualitative indicators so that the evaluation results are more credible. The evaluation of cost management of micro and small manufacturing enterprises based on the supply chain was carried out by using gray fuzzy analysis, and the empirical analysis was based on the research data of company B. The evaluation result was "poor," which verified the applicability of the cost management performance evaluation model of micro and small manufacturing enterprises and indicated the direction for the improvement of cost management of micro and small manufacturing enterprises. And, the proposed intuitionistic fuzzy hierarchical analysis has greater advantages in evaluation accuracy than the traditional fuzzy hierarchical analysis.

Recent advances of oral film as platform for drug delivery.

Orally drug delivery film has received extensive interest duo to a distinct set of its advantageous properties compared to the traditional orally administered dosages, including faster rate of drug absorption, higher bioavailability and better patient compliance for children and elders with swallowing deficiencies. In particular, its potential capacity of delivering proteins and peptides has further attracted great attention. Lately, tremendous advances have been made in designing and developing both novel mucoadhesive films and orodispersible films to fulfill specific accomplishments of drug delivery. This review aims to summarize those newly developed oral films, discussing their formulation strategies, manufacturing methods as well as advantages and limitations thereof. Conclusions and future perspectives are also provided in brief.