ADAMTS13 deficiency exacerbates neuroinflammation by targeting matrix metalloproteinase-9 in ischemic brain injury.

Our design aimed to explore the potential involvement of matrix metalloproteinase-9 (MMP-9) in the inflammatory response associated with acute ischemic stroke (AIS). We also aimed to preliminarily examine the potential impact of a disintegrin-like and metalloprotease with thrombospondin type I repeats-13 (ADAMTS13) on MMP-9 in AIS. We conducted oxygen-glucose deprivation models of microglia cells and mice models of AIS with middle cerebral artery occlusion (MCAO). We assessed the expression pattern of MMP-9 with western blotting (WB) and real-time quantitative PCR both in vivo and in vitro. MMP-9 downregulation was achieved by using ACE inhibitors such as trandolapril. For the MCAO model, we used ADAMTS13-deficient mice. We then evaluated the related neurological function scores, cerebral edema and infarct volume. The levels of inflammation-related proteins, such as COX2 and iNOS, were assessed using WB, and the expression of inflammatory cytokines was measured via enzyme-linked immuno sorbent assay in vivo. Our findings indicated that MMP-9 was up-regulated while ADAMTS13 was down-regulated in the MCAO model. Knockdown of MMP-9 reduced both inflammation and ischemic brain injury. ADAMTS13 prevented brain damage, improved neurological function and decreased the inflammation response in mice AIS models. Additionally, ADAMTS13 alleviated MMP-9-induced neuroinflammation in vivo. It showed that ADAMTS13 deficiency exacerbated ischemic brain injury through an MMP-9-dependent inflammatory mechanism. Therefore, the ADAMTS13-MMP-9 axis could have therapeutic potential for the treatment of AIS.

Preparation and Performance Analysis of Ceramsite Asphalt Mixture with Phase-Change Material.

In this paper, phase-change material (PCM) and ceramsite were used to increase the heat resistance of the asphalt mixture. The ceramsite asphalt mixture with PCM can bring a specific cooling effect to the road surface and alleviate the rapid deterioration at high temperature. Two phase-change materials, PCM-43 and PCM-48, were compared and selected as the heat absorption material of the asphalt mixture. It is found that PCM-43 has better thermal stability, temperature regulation performance, higher enthalpy value, and a less adverse effect on the rheological properties of asphalt. According to the road performance of the asphalt mixture, it suggests that the maximum content of ceramsite is 40%. The specific heat capacity of asphalt mixtures was studied by the method of the insulation bucket test, and the thermal conductivity coefficient of asphalt mixtures was tested by a thermal conductivity instrument. The results show that the specific heat capacity and thermal conductivity of the asphalt mixture can be reduced by adding PCM and ceramsite. The effect of ceramsite asphalt concrete with PCM on the temperature field of road structure was further analyzed by finite element method. Due to the thermal resistance of ceramsite in the upper layer, the cooling range and depth in the middle and lower surface layers can be improved. Meanwhile, the heat absorption of phase-change material can alleviate the heating phenomenon of the upper layer. Therefore, ceramsite asphalt concrete with PCM is effective for decreasing the high temperatures in the asphalt pavements.

Cerebral metabolic rate of oxygen (CMRO2) changes measured with simultaneous tDCS-MRI in healthy adults.

BACKGROUND: Transcranial direct current stimulation (tDCS) is a safe and well-tolerated noninvasive technique used for cortical excitability modulation. tDCS has been extensively investigated for its clinical applications; however further understanding of its underlying in-vivo physiological mechanisms remains a fundamental focus of current research. OBJECTIVES: We investigated the simultaneous effects of tDCS on cerebral blood flow (CBF), venous blood oxygenation (Yv) and cerebral metabolic rate of oxygen (CMRO2) using simultaneous MRI in healthy adults to provide a reference frame for its neurobiological mechanisms. METHODS: Twenty-three healthy participants (age = 35.6 ± 15.0 years old, 10 males) completed a simultaneous tDCS-MRI session in a 3 T scanner fitted with a 64-channels head coil. A MR-compatible tDCS device was used to acquire CBF, Yv and CMRO2 at three time points: pre-, during- and post- 15 minutes of 2.0 mA tDCS on left anodal dorsolateral prefrontal cortex. RESULTS: During tDCS, CBF significantly increased (57.10 ± 8.33 mL/100g/min) from baseline (53.67 ± 7.75 mL/100g/min; p < 0.0001) and remained elevated in post-tDCS (56.79 ± 8.70 mL/100g/min). Venous blood oxygenation levels measured in pre-tDCS (60.71 ± 4.12 %) did not significantly change across the three timepoints. The resulting CMRO2 significantly increased by 5.9 % during-tDCS (175.68 ± 30.78 µmol/100g/min) compared to pre-tDCS (165.84 ± 25.32 µmol/100g/min; p = 0.0015), maintaining increased levels in post-tDCS (176.86 ± 28.58 µmol/100g/min). CONCLUSIONS: tDCS has immediate effects on neuronal excitability, as measured by increased cerebral blood supply and oxygen consumption supporting increased neuronal firing. These findings provide a standard range of CBF and CMRO2 changes due to tDCS in healthy adults that may be incorporated in clinical studies to evaluate its therapeutic potential.

Peat and bentonite amendments assisted soilless revegetation of oligotrophic and heavy metal contaminated nonferrous metallic tailing.

Soilless revegetation is a promising method for ecological restoration of nonferrous metallic tailings because of its low-cost and eco-friendliness. However, revegetation is difficult to construct in the tailings due to the high heavy metal concentration, poor water retention capacity and low fertility. In this study, soilless revegetation was successfully carried out by using peat and bentonite amendments. The results showed that amendment addition significantly increased the F.elata seed germination percentage, plant length and fresh biomass by 14.9%-24.3%, 48.9%-90.4% and 51.9%-88.1%, respectively. Such improvements probably referred to the variation of rhizosphere tailing microecological characteristics. Amendment addition dramatically improved tailing available NPK by 39.76-102.13%, 2.69-40.81% and 2.42-20.02%, respectively, and reduced pH from alkaline to relative neutral. Besides, heavy metal bioavailability was significantly decreased that the acid soluble fraction decreased by 1.7%-11.5%, resulting in the reduction of heavy metal concentration in F.elata plant. Amendments also increased the rhizosphere tailing microbial species richness and the relative abundance of ecologically beneficial genera including Arthrobacter, Altererythrobacter and Bacillus. This study not only provided a green and efficient method for remediation of oligotrophic and high heavy metal contaminated nonferrous metallic tailing, but also demonstrated relevant mechanisms of amendment on promoting soilless revegetation.

Fatigue behaviour and biocompatibility of additively manufactured bioactive tantalum graded lattice structures for load-bearing orthopaedic applications.

Laser powder bed fusion (LPBF) additive manufacturing of pure tantalum and their graded lattice structures was systematically investigated, with emphasis on their microstructure evolution, phase formation, surface energy and biological properties in comparison with conventionally forged pure Ta. The LPBF fabricated Ta (LPBF-Ta) exhibited lower contact angles and higher surface energy than the forged-Ta which indicated the better wettability of the LPBF-Ta. The adhesion and proliferation of rat bone marrow stromal cells (rBMSCs) were also enhanced for the LPBF-Ta when compared to forged-Ta. Three different Ta graded gyroid lattice structures (i.e., uniform structure, Y-gradient structure, Z-gradient structure) were designed and fabricated using the same optimised LPBF parameters. Y-gradient structures exhibited the best plateau stress and compressive modulus among three different graded structures due to the maximum local volume fraction on the fracture plane. In fatigue response, Y-gradient outperformed the other two gyroid structures under varying stresses. In terms of cell culture response, the uniform structures performed the best biocompatibility due to its suitable pore size for cell adhesion and growth. This study provides new and in-depth insights into the LPBF additive manufacturing of pure Ta graded lattice structures with desired fatigue and biological properties for load-bearing orthopaedic applications.

Method for evaluating spectral resolution based on a single-lens spectrometer.

Spectral resolution is a key parameter of a spectrometer. Typically, the Rayleigh criterion is used to evaluate spectral resolution; however, it is not applicable to a single-lens-based spectrometer, as its principle is different from that using a prism or grating. Therefore, this work proposes that a method resolution is a key parameter to evaluate spectral resolution by exploiting the concept of focus depth. Accordingly, the spectral resolution is determined using three factors, namely, aperture factor F of the lens, pixel size p of the charge-coupled device, and the derivative of the rate of change of focal length with respect to wavelength f'(λ). The proposed method is verified by simulations with the following lens parameters: a diameter of 50.8 mm, focal length of 200 mm at 587.6 nm, and F=3.94. The calculated and simulated spectral resolution values are, respectively, 1.7 nm and 1.2 nm at 480 nm. Based on an analysis of the influences of F, p, and f'(λ) on the spectral resolution, increasing f'(λ) or decreasing both F and p might improve the spectral resolution. Finally, the proposed method is validated via experiments for lenses with different F values as well as materials, and we determine their spectral resolutions; these results are observed to be similar to the calculated values.

Spectral acquisition method based on axial chromatic and spherical aberrations of lens.

In this study, a spectral acquisition method is proposed in which axial chromatic and spherical aberrations are introduced as an error function. These aberrations lead to changes in the focal lengths as the wavelengths of the incident light changes. A coefficient matrix representing the variation in the intensity distribution of each image, formed at the focal point (the detection position) corresponding to a wavelength, is obtained by calibration. The least square method is used to reconstruct the spectrum. The numerical simulation results show that the spectral correlation coefficient and the spectral mean square error between the reconstructed spectrum and the original spectrum are 0.9997 and 0.0025, and 0.9683 and 0.0204, respectively, for the polychromatic light spectrum obtained from the mercury lamp using our experimental set-up. These results confirm the feasibility and efficiency of the proposed spectral imaging method.