[Occurrence and resistance of bacteria isolated from the rabbit nares - a retrospective evaluation]. / Vorkommen und Resistenzen von Bakterien in Proben aus Kaninchennasen ­ eine retrospektive Auswertung.

OBJECTIVE: Rabbit snuffles is one of the most common challenges in veterinary practice. The aim of the present study was to evaluate nasal samples of rabbits submitted between 2015-2019, with regard to bacterial distribution and antimicrobial resistance. MATERIAL AND METHODS: Each sample was plated on four different agar plates and enriched in a non-selective broth. Isolates were identified by MALDI Biotyper® (MBT) (Bruker Daltonik GmbH, Bremen, Germany) and antimicrobial susceptibility was performed by broth microdilution method in accordance with the Clinical and Laboratory Standards Institute (CLSI, Wayne, PA, USA). RESULTS: A total of 1261 samples were evaluated. Among the samples that tested positive (n=941), one bacterial species was detected in 79.1% of the cases, and more than one bacterial species (mixed culture) was found in 20.9% of the cases. A total of 150 species from 14 families were identified. Isolates belonging to the family Pasteurellaceae were identified most frequently, followed by Enterobacteriaceae, Pseudomonadaceae and Staphylococcaceae.A total of 467 antibiograms of the most common pathogens with possible clinical relevance (Pasteurella multocida [14.6%], Pasteurella species [10.0%], Staphylococcus aureus [5.9%], Pseudomonas aeruginosa [5.4%] and Bordetella bronchiseptica [4.8%]) were evaluated. Quinolones showed the highest efficacy and clindamycin the lowest. Furthermore, among S. aureus, MRSA were most frequently detected in 2016 reaching 23.1% of cases. CONCLUSIONS AND CLINICAL RELEVANCE: Since the causal bacteria for rabbit snuffles are mostly found in the deeper areas of the nose and the nasal vestibule is often contaminated with ubiquitous and coliform bacteria, it would make sense to take samples from the depth of the nasal cavity, ideally via nasal lavage. Due to the demonstrated pathogen diversity and long-term therapy associated with the disease, bacterial culture and sensitivity testing is recommended as part of the management. In the absence of an antibiogram, enrofloxacin is the drug of first choice due to its favorable resistance pattern and tolerability. However, since quinolones are considered as "critically important" antibiotics, their use should be limited to a minimum.

A comparison of manual counting of rabbit reticulocytes with ADVIA 2120i analyzer counting.

We compared manual counting of reticulocytes in rabbits with automatic counting using an ADVIA 2120i analyzer. Reproducibility and the influence of different anticoagulants (EDTA and Li-heparin) were also examined. Blood samples of 331 rabbits (method comparison, n = 289; reproducibility, n = 33; comparison of anticoagulants, n = 9) were tested. The reticulocyte numbers of each specimen were manually determined twice for method comparison. Passing-Bablok regressions, Bland-Altman plots, and the coefficient of variation (CV) were used to evaluate statistical significance. Good correlation (rs = 0.81) was observed between manual reticulocyte counting (groups 1-4) and the ADVIA 2120i. Quantification with the ADVIA 2120i was reproducible for relative reticulocyte numbers (EDTA, CV = 4.24%; Li-heparin, CV = 3.63%) and absolute reticulocyte numbers (EDTA, CV = 5.64%; Li-heparin, CV = 3.81%). The absolute and relative reticulocyte numbers were significantly higher in Li-heparin samples than in EDTA samples (absolute, p = 0.009; relative, p = 0.016). The ADVIA 2120i is suitable for counting reticulocytes in rabbit blood samples, but reticulocyte numbers are higher by manual counting than by ADVIA 2120i counting. Therefore, microscopic confirmation of quantifications is recommended when high numbers of reticulocytes are observed. The anticoagulant of choice is EDTA.

Clinical evaluation of therapeutic success in rabbits with suspected encephalitozoonosis.

Between 2000 and 2008, 95 rabbits with suspected encephalitozoonosis and neurological symptoms were treated at the Clinic of Small Animal Medicine, Ludwig Maximilian University Munich. Standard treatment consisted of oxytetracycline (from 2000 to 2003; n=50) or fenbendazole and oxytetracycline (from 2004 to 2008; n=45), and the rabbits were randomly assigned to treatment groups with or without dexamethasone. Each therapeutic regime was given for 10 days, with fluids, B vitamins and nutritional support added as needed. Therapeutic success was evaluated by assessing the survival rate on day 10, the neurological score of the surviving rabbits and Kaplan-Meier curves for long-term survival past 10 days. Inclusion of fenbendazole in the treatment protocol was associated with increased survival rates on day 10 (p=0.043), better neurological scores (p=0.008), and improved long-term survival (p=0.025) based on the results of univariate analyses. Treatment with dexamethasone showed no effect on neurological score or on short- or long-term survival. The study did not provide any evidence that dexamethasone is an effective component of the treatment scheme.