RESUMEN
AIMS: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia in man, causing substantial morbidity and mortality with a major worldwide public health impact. It is increasingly recognized as a highly heritable condition. This study aimed to determine genetic risk factors for early-onset AF. METHODS AND RESULTS: We sequenced the whole genomes of 8453 Icelanders and imputed genotypes of the 25.5 million sequence variants we discovered into 1799 Icelanders with early-onset AF (diagnosed before 60 years of age) and 337 453 controls. Each sequence variant was tested for association based on multiplicative and recessive inheritance models. We discovered a rare frameshift deletion in the myosin MYL4 gene (c.234delC) that associates with early-onset AF under a recessive mode of inheritance (allelic frequency = 0.58%). We found eight homozygous carriers of the mutation, all of whom had early-onset AF. Six of the homozygotes were diagnosed by the age of 30 and the remaining two in their 50s. Three of the homozygotes had received pacemaker implantations due to sick sinus syndrome, three had suffered an ischemic stroke, and one suffered sudden cardiac death. CONCLUSIONS: Through a population approach we found a loss of function mutation in the myosin gene MYL4 that, in the homozygous state, is completely penetrant for early-onset AF. The finding may provide novel mechanistic insight into the pathophysiology of this complex arrhythmia.
Asunto(s)
Fibrilación Atrial/genética , Mutación del Sistema de Lectura/genética , Cadenas Ligeras de Miosina/genética , Anciano , Fibrilación Atrial/etnología , Estudios de Casos y Controles , Muerte Súbita Cardíaca/etnología , Muerte Súbita Cardíaca/etiología , Femenino , Eliminación de Gen , Genes Recesivos/genética , Estudio de Asociación del Genoma Completo/métodos , Heterocigoto , Homocigoto , Humanos , Islandia/etnología , Masculino , Persona de Mediana Edad , Linaje , Factores de Riesgo , Sarcómeros , Alineación de Secuencia/métodos , Síndrome del Seno Enfermo/etnología , Síndrome del Seno Enfermo/genética , Accidente Cerebrovascular/etnología , Accidente Cerebrovascular/genéticaRESUMEN
Here we describe the insights gained from sequencing the whole genomes of 2,636 Icelanders to a median depth of 20×. We found 20 million SNPs and 1.5 million insertions-deletions (indels). We describe the density and frequency spectra of sequence variants in relation to their functional annotation, gene position, pathway and conservation score. We demonstrate an excess of homozygosity and rare protein-coding variants in Iceland. We imputed these variants into 104,220 individuals down to a minor allele frequency of 0.1% and found a recessive frameshift mutation in MYL4 that causes early-onset atrial fibrillation, several mutations in ABCB4 that increase risk of liver diseases and an intronic variant in GNAS associating with increased thyroid-stimulating hormone levels when maternally inherited. These data provide a study design that can be used to determine how variation in the sequence of the human genome gives rise to human diversity.
Asunto(s)
Subfamilia B de Transportador de Casetes de Unión a ATP/genética , Subunidades alfa de la Proteína de Unión al GTP Gs/genética , Cadenas Ligeras de Miosina/genética , Anciano , Anciano de 80 o más Años , Fibrilación Atrial/genética , Parálisis Bulbar Progresiva/genética , Cromograninas , Femenino , Mutación del Sistema de Lectura , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Genoma Humano , Estudio de Asociación del Genoma Completo , Pérdida Auditiva Sensorineural/genética , Humanos , Mutación INDEL , Islandia , Hepatopatías/genética , Masculino , Persona de Mediana Edad , Anotación de Secuencia Molecular , Filogeografía , Polimorfismo de Nucleótido Simple , Receptores Acoplados a Proteínas G/genética , Riesgo , Análisis de Secuencia de ADN , Tirotropina/sangreRESUMEN
Renal cell carcinoma (RCC) represents between 80 and 90% of kidney cancers. Previous genome-wide association studies of RCC have identified five variants conferring risk of the disease. Here we report the results from a discovery RCC genome-wide association study and replication analysis, including a total of 2,411 patients and 71,497 controls. One variant, rs35252396[CG] located at 8q24.21, is significantly associated with RCC after combining discovery and replication results (OR=1.27, P(combined)=5.4 × 10(-11)) and has an average risk allele frequency in controls of 46%. rs35252396[CG] does not have any strongly correlated variants in the genome and is located within a region predicted to have regulatory functions in several cell lines, including six originating from the kidney. This is the first RCC variant reported at 8q24.21 and it is largely independent (r(2)≤0.02) of the numerous previously reported cancer risk variants at this locus.
Asunto(s)
Carcinoma de Células Renales/genética , Predisposición Genética a la Enfermedad , Neoplasias Renales/genética , Polimorfismo de Nucleótido Simple , Anciano , Carcinoma de Células Renales/epidemiología , Biología Computacional , Regulación Neoplásica de la Expresión Génica , Genoma Humano , Genotipo , Humanos , Islandia/epidemiología , Neoplasias Renales/epidemiología , Oportunidad Relativa , Factores de RiesgoRESUMEN
In Western countries, prostate cancer is the most prevalent cancer of men and one of the leading causes of cancer-related death in men. Several genome-wide association studies have yielded numerous common variants conferring risk of prostate cancer. Here, we analyzed 32.5 million variants discovered by whole-genome sequencing 1,795 Icelanders. We identified a new low-frequency variant at 8q24 associated with prostate cancer in European populations, rs188140481[A] (odds ratio (OR) = 2.90; P(combined) = 6.2 × 10(-34)), with an average risk allele frequency in controls of 0.54%. This variant is only very weakly correlated (r(2) ≤ 0.06) with previously reported risk variants at 8q24, and its association remains significant after adjustment for all known risk-associated variants. Carriers of rs188140481[A] were diagnosed with prostate cancer 1.26 years younger than non-carriers (P = 0.0059). We also report results for a previously described HOXB13 variant (rs138213197[T]), confirming it as a prostate cancer risk variant in populations from across Europe.
Asunto(s)
Adenocarcinoma/genética , Mutación , Neoplasias de la Próstata/genética , Anciano , Anciano de 80 o más Años , Secuencia de Bases , Línea Celular , Cromosomas Humanos Par 8 , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Genoma Humano , Estudio de Asociación del Genoma Completo , Proteínas de Homeodominio/genética , Humanos , Islandia , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Polimorfismo de Nucleótido Simple , Riesgo , Análisis de Secuencia de ADN , Población Blanca/genéticaRESUMEN
To search for sequence variants conferring risk of nonmedullary thyroid cancer, we focused our analysis on 22 SNPs with a P < 5 × 10(-8) in a genome-wide association study on levels of thyroid stimulating hormone (TSH) in 27,758 Icelanders. Of those, rs965513 has previously been shown to associate with thyroid cancer. The remaining 21 SNPs were genotyped in 561 Icelandic individuals with thyroid cancer (cases) and up to 40,013 controls. Variants suggestively associated with thyroid cancer (P < 0.05) were genotyped in an additional 595 non-Icelandic cases and 2,604 controls. After combining the results, three variants were shown to associate with thyroid cancer: rs966423 on 2q35 (OR = 1.34; P(combined) = 1.3 × 10(-9)), rs2439302 on 8p12 (OR = 1.36; P(combined) = 2.0 × 10(-9)) and rs116909374 on 14q13.3 (OR = 2.09; P(combined) = 4.6 × 10(-11)), a region previously reported to contain an uncorrelated variant conferring risk of thyroid cancer. A strong association (P = 9.1 × 10(-91)) was observed between rs2439302 on 8p12 and expression of NRG1, which encodes the signaling protein neuregulin 1, in blood.
Asunto(s)
Cromosomas Humanos Par 14/genética , Cromosomas Humanos Par 2/genética , Cromosomas Humanos Par 8/genética , Sitios Genéticos/genética , Predisposición Genética a la Enfermedad/genética , Neoplasias de la Tiroides/genética , Tirotropina/metabolismo , Variación Genética , Estudio de Asociación del Genoma Completo , Genotipo , Humanos , Islandia , Neurregulina-1/sangre , Neurregulina-1/genética , Polimorfismo de Nucleótido Simple/genéticaRESUMEN
Through complementary application of SNP genotyping, whole-genome sequencing and imputation in 38,384 Icelanders, we have discovered a previously unidentified sick sinus syndrome susceptibility gene, MYH6, encoding the alpha heavy chain subunit of cardiac myosin. A missense variant in this gene, c.2161C>T, results in the conceptual amino acid substitution p.Arg721Trp, has an allelic frequency of 0.38% in Icelanders and associates with sick sinus syndrome with an odds ratio = 12.53 and P = 1.5 × 10⻲9. We show that the lifetime risk of being diagnosed with sick sinus syndrome is around 6% for non-carriers of c.2161C>T but is approximately 50% for carriers of the c.2161C>T variant.
