RESUMEN
Alexander disease is a rare disorder of cerebral white matter due to a dysfunction of astrocytes. The most common infantile form presents as a megalencephalic leukodystrophy. Mutations of the GFAP gene, encoding Glial Fibrillary Acidic Protein, have been recognized as the cause of Alexander disease. Glial Fibrillary Acidic Protein is the major intermediate filament protein in astrocytes, its functional rod domain is conserved in sequence and structure among other intermediate filament proteins. We report here two cases of infantile Alexander disease with early onset and severe course, caused by DE NOVO mutations A364 V and Y366C. Both affected GFAP residues are part of a highly conserved coiled-coil trigger motif in the C-terminal end of segment 2B, probably required for the stability of intermediate filament molecules. Comparable effects are seen with mutations of the corresponding residues of the gene coding for keratin 14, another intermediate filament, this further supports the hypothesis that these positions of the trigger motif are generally critical for a normal function of intermediate filaments.
Asunto(s)
Enfermedad de Alexander/genética , Exones/genética , Proteína Ácida Fibrilar de la Glía/genética , Mutación/genética , Edad de Inicio , Alanina/genética , Enfermedad de Alexander/patología , Cisteína/genética , Análisis Mutacional de ADN/métodos , Femenino , Lóbulo Frontal/patología , Humanos , Lactante , Imagen por Resonancia Magnética/métodos , Masculino , Estructura Terciaria de Proteína/genética , Tirosina/genética , Valina/genéticaAsunto(s)
Cromosomas Humanos X , Duplicación de Gen , Proteína 2 de Unión a Metil-CpG/genética , Síndrome de Rett/diagnóstico , Síndrome de Rett/genética , Niño , Femenino , Dosificación de Gen , Expresión Génica , Humanos , Hibridación Fluorescente in Situ , Masculino , Proteína 2 de Unión a Metil-CpG/metabolismo , LinajeRESUMEN
The transcription factor-like nuclear regulator (TFNR) is a novel human gene that maps on 5q13, distal to the duplicated region that includes SMN1, the spinal muscular atrophy (SMA) determining gene. The location of TFNR allowed us to design an evolutionary model of the SMA region. The 9.5-kb TFNR transcript is highly expressed in cerebellum and weakly in all other tissues tested. TFNR encodes a protein of 2254 amino acids (aa) and contains nine repeats of a novel 55-aa motif, of yet unknown function. The coding region is organized in 32 exons. Alternative splicing of exon 15 results in a truncated protein of 796 aa. TFNR comprises a series of polypeptides that range from 55 to 250 kDa. Immunocytological studies showed that the TFNR protein is present exclusively in the nucleus, where it is concentrated in several nuclear structures. Amino acids 155-474 show significant homology to TFC5, a subunit of the yeast transcription factor TFIIIB, suggesting that TFNR is a putative transcription factor. Based on its proximity to SMN1 and its expression pattern, TFNR may be a candidate gene for atypical forms of SMA with cerebral atrophy and axonal neuropathy that have been shown to carry large deletions in the SMA region.
