Ecotoxicological evaluation of chitosan biopolymer films particles in adult zebrafish (Danio rerio): A comparative study with polystyrene microplastics.

To mitigate the environmental impact of microplastics (MPs), the scientific community has innovated sustainable and biodegradable polymers as viable alternatives to traditional plastics. Chitosan, the deacetylated form of chitin, stands as one of the most thoroughly investigated biopolymers and has garnered significant interest due to its versatile applications in both medical and cosmetic fields. Nevertheless, there is still a knowledge gap regarding the impact that chitosan biopolymer films (CBPF) may generate in aquatic organisms. In light of the foregoing, this study aimed to assess and compare the potential effects of CBPF on the gastrointestinal tract, gills, brain, and liver of Danio rerio against those induced by MPs. The findings revealed that both CBPF and MPs induced changes in the levels of oxidative stress biomarkers across all organs. However, it is essential to note that our star plots illustrate a tendency for CBPF to activate antioxidant enzymes and for MPs to produce oxidative damage. Regarding gene expression, our findings indicate that MPs led to an up-regulation in the expression of genes associated with apoptotic response (p53, casp3, cas9, bax, and bcl2) in all fish organs. Meanwhile, CBPF produced the same effect in genes related to antioxidant response (nrf1 and nrf2). Overall, our histological observations substantiated these effects, revealing the presence of plastic particles and tissue alterations in the gills and gastrointestinal tract of fish subjected to MPs. From these results, it can be concluded that CBPF does not represent a risk to fish after long exposure.

Comparison of the SMLM technique and the MSSR algorithm in confocal microscopy for super-resolved imaging of cellulose fibres.

Nowadays, the use of super-resolution microscopy (SRM) is increasing globally due to its potential application in several fields of life sciences. However, a detailed and comprehensive guide is necessary for understanding a single-frame image's resolution limit. This study was performed to provide information about the structural organisation of isolated cellulose fibres from garlic and agave wastes through fluorophore-based techniques and image analysis algorithms. Confocal microscopy provided overall information on the cellulose fibres' microstructure, while techniques such as total internal reflection fluorescence microscopy facilitated the study of the plant fibres' surface structures at a sub-micrometric scale. Furthermore, SIM and single-molecule localisation microscopy (SMLM) using the PALM reconstruction wizard can resolve the network of cellulose fibres at the nanometric level. In contrast, the mean shift super-resolution (MSSR) algorithm successfully determined nanometric structures from confocal microscopy images. Atomic force microscopy was used as a microscopy technique for measuring the size of the fibres. Similar fibre sizes to those evaluated with SIM and SMLM were found using the MSSR algorithm and AFM. However, the MSSR algorithm must be cautiously applied because the selection of thresholding parameters still depends on human visual perception. Therefore, this contribution provides a comparative study of SRM techniques and MSSR algorithm using cellulose fibres as reference material to evaluate the performance of a mathematical algorithm for image processing of bioimages at a nanometric scale. In addition, this work could act as a simple guide for improving the lateral resolution of single-frame fluorescence bioimages when SRM facilities are unavailable.

Recent Developments in Synthesis, Properties, Applications and Recycling of Bio-Based Elastomers.

In 2021, global plastics production was 390.7 Mt; in 2022, it was 400.3 Mt, showing an increase of 2.4%, and this rising tendency will increase yearly. Of this data, less than 2% correspond to bio-based plastics. Currently, polymers, including elastomers, are non-recyclable and come from non-renewable sources. Additionally, most elastomers are thermosets, making them complex to recycle and reuse. It takes hundreds to thousands of years to decompose or biodegrade, contributing to plastic waste accumulation, nano and microplastic formation, and environmental pollution. Due to this, the synthesis of elastomers from natural and renewable resources has attracted the attention of researchers and industries. In this review paper, new methods and strategies are proposed for the preparation of bio-based elastomers. The main goals are the advances and improvements in the synthesis, properties, and applications of bio-based elastomers from natural and industrial rubbers, polyurethanes, polyesters, and polyethers, and an approach to their circular economy and sustainability. Olefin metathesis is proposed as a novel and sustainable method for the synthesis of bio-based elastomers, which allows for the depolymerization or degradation of rubbers with the use of essential oils, terpenes, fatty acids, and fatty alcohols from natural resources such as chain transfer agents (CTA) or donors of the terminal groups in the main chain, which allow for control of the molecular weights and functional groups, obtaining new compounds, oligomers, and bio-based elastomers with an added value for the application of new polymers and materials. This tendency contributes to the development of bio-based elastomers that can reduce carbon emissions, avoid cross-contamination from fossil fuels, and obtain a greener material with biodegradable and/or compostable behavior.

Recent developments in bio-based polyethylene: Degradation studies, waste management and recycling.

Nowadays, the tendency to replace conventional fossil-based plastics is increasing considerably; there is a growing trend towards alternatives that involve the development of plastic materials derived from renewable sources, which are compostable and biodegradable. Indeed, only 1.5 % of whole plastic production is part of the small bioplastics market, even when these materials with a partial or full composition from biomass are rapidly expanding. A very interesting field of investigation is currently being developed in which the disposal and processing of the final products are evaluated in terms of reducing environmental harm. This review presents a compilation of polyethylene (PE) types, their uses, and current problems in the waste management of PE and recycling. Particularly, this review is based on the capabilities to synthesize bio-based PE from natural and renewable sources as a replacement for the raw material derived from petroleum. In addition to recent studies in degradation on different types of PE with weight loss ranges from 1 to 47 %, the techniques used and the main changes observed after degradation. Finally, perspectives are presented in the manuscript about renewable and non-renewable polymers, depending on the non-degradable, biodegradable, and compostable behavior, including composting recent studies in PE. In addition, it contributes to the 3R approaches to responsible waste management of PE and advancement towards an environmentally friendly PE.

Polystyrene microplastics mitigate the embryotoxic damage of metformin and guanylurea in Danio rerio.

Microplastics (MPs) alone may endanger the health and fitness of aquatic species through different mechanisms. However, the harmful effects of these when mixed with other emerging contaminants require additional research. Herein, we aimed to determine whether a mixture of MPs with metformin (MET) or guanylurea (GUA) might induce embryotoxicity and oxidative stress in Danio rerio. Upon exposure to mixtures, our results showed MPs reduced the mortality rate of MET and GUA in embryos. Moreover, the severity and the rate of malformations were also decreased in all mixtures with MPs. Concerning oxidative stress, our findings indicated MET, GUA, MPs, and the mixtures increased the levels of lipoperoxidation, hydroperoxide content, and protein carbonyl content in D. rerio larvae. However, the oxidative damage induced in all mixtures was lower than that produced by both drugs alone. Thus, it is likely that the accumulation of MPs avoided the entrance of MET and GUA into the embryos. Once the embryo hatched, MPs did only remain accumulated in the yolk sac of larvae and did not translocate to other organs. Our risk assessment analysis confirmed that MPs shrunk the damage produced by MET and GUA. In a nutshell, MPs mitigate the embryotoxic damage of metformin and guanylurea in D. rerio by blocking their entrance.

Effect of ball milling on cellulose nanoparticles structure obtained from garlic and agave waste.

The aim of this study was to obtain cellulose and cellulose nanoparticles (CNP) from garlic and agave wastes, as well as elucidating its structure at different scales using microscopy and spectroscopy techniques. Cellulose is isolated by using a sequential extractive process and monitored by CLSM and SEM, while CNP are produced in a high-energy planetary mill. FTIR and XRD confirmed the presence of cellulose type I and CI and Dhkl was used to evaluate the size of CNP. The corresponding crystalline structure, d-spacing and angles obtained from crystalline regions of CNP were estimated by TEM and computational simulation. It is shown that the triclinic phase is predominant in G, and a monoclinic conformation in CNP for A. The novelty of this contribution is to demonstrate that the crystalline structure of CNP, extracted from different agro-food wastes, depends on its initial microstructural arrangement (laminar or fibrillar).