An application example of the likelihood ratio approach to the evaluation of organic gunshot residues using a fictional scenario and recently published data.

The analysis of gunshot residues (GSR) can provide important information with regard to the involvement of a person of interest (POI) in a firearm-related incident. Organic gunshot residues (OGSR) have been investigated in order to provide additional and complementary information to the traditional inorganic gunshot residue (IGSR) particles detected by scanning electron microscopy (SEM). Currently, many procedures and analytical methods have been developed to detect OGSR-related compounds collected from the shooter's hands. However, such studies provide no information regarding the inclusion of such results in an activity level evaluation for discharging a firearm. The aim of this article is to assess the feasibility of using the likelihood ratio (LR) approach as a tool to evaluate OGSR results for activity level propositions. The developed model focuses on the assignment of an LR for several compounds detected in OGSR. A simple worst-case simulation was investigated in order to assess the applicability of the LR approach to evaluate OGSR traces. This simulation highlighted the importance of addressing an appropriate pair of activity level propositions when evaluating the results.

Evaluating the effect of barrel length on pellet distribution patterns of sawn-off shotguns.

In recent years, sawn-off shotguns have been regularly encountered in firearm-related incidents. Shotgun represents a community threat due to their ability to be shortened in order to improve their concealability. In practice, forensic scientists and investigators may be asked to determine if a shotgun used in a particular event might have been shortened based on the pellet distribution. To investigate the effect of altering the barrel length on pellet distribution, five 12-gauge shotguns were progressively shortened. Each shotgun was assessed at six barrel lengths and a single discharge of No. 4 shot shotshell ammunition was fired at a fixed fabric target. The shotguns were positioned at six different distances from target and experiments conducted in triplicate. The fabric targets containing pellet pattern underwent data analysis to calculate the pellet distribution area. At short distances from target (≤ 1.0 m), the pellet penetration was observed as a single entry point as the pellets did not have a sufficient flight time to spread from each other. For distances of 2.5 m, 5 m and 10 m, pellet penetration patterns were observed and a quantitative analysis was performed on the samples containing pellet distribution patterns. At each of these distances, it was detected that there was a noticeable increase in pellet distribution area between the unaltered shotgun and altered barrel lengths for all shotguns. The distance from the target as well as the presence of a choke in the shotgun's barrel was found to have a greater impact on the pellet distribution than the barrel length.

A forensic investigation on the persistence of organic gunshot residues.

Gunshot residues (GSR) are a potential form of forensic traces in firearm-related events. In most forensic laboratories, GSR analyses focus on the detection and characterisation of the inorganic components (IGSR), which are mainly particles containing mixtures of lead, barium and antimony originating from the primer. The increasing prevalence of heavy metal-free ammunition challenges the current protocols used for IGSR analysis. To provide complementary information to IGSR particles, the current study concentrated on the organic components (OGSR) arising from the combustion of the propellant. The study focused on four compounds well-known as being part of OGSR: ethylcentralite (EC), methylcentralite (MC), diphenylamine (DPA), N-nitrosodiphenylamine (N-nDPA). This study assessed the retention of these OGSR traces on a shooter's hands. The overall project aim was to provide appropriate information regarding OGSR persistence, which can be suitable to be integrated into the interpretation framework of OGSR as recommended by the recent ENFSI Guideline for Evaluative Reporting in Forensic Science. The persistence was studied through several intervals ranging from immediately after discharge to four hours and two ammunition calibres were chosen: .40 S&W calibre, used by the NSW Police Force; and .357 Magnum, which is frequently encountered in Australian casework. This study successfully detected the compounds of interest up to four hours after discharge. The trends displayed a large decrease in the amount detected during the first hour. A large variability was also observed due to numerous factors involved in the production, deposition and collection of OGSR.

The development and comparison of collection techniques for inorganic and organic gunshot residues.

The detection and interpretation of gunshot residues (GSR) plays an important role in the investigation of firearm-related events. Commonly, the analysis focuses on inorganic particles incorporating elements derived from the primer. However, recent changes in ammunition formulations and possibility that particles from non-firearm sources can be indistinguishable from certain primer particles challenge the standard operational protocol and call for adjustments, namely the combination of inorganic and organic GSR analysis. Two protocols for the combined collection and subsequent analysis of inorganic and organic GSR were developed and optimised for 15 compounds potentially present in organic GSR (OGSR). These protocols were conceptualised to enable OGSR analysis by ultrahigh-performance liquid chromatography (UHPLC) coupled with UV detection and triple quadrupole tandem mass spectrometry (confirmation) and IGSR analysis by scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDX). Using liquid extraction, the extraction recoveries from spiked swabs and stubs were ~80 % (50-98 % for swabs, 64-98 % for stubs). When the mixed OGSR standard was applied to the hands and recovered in the way that is usual for IGSR collection, GSR stubs performed significantly better than swabs (~30 %) for the collection of OGSR. The optimised protocols were tested and compared for combined OGSR and inorganic GSR analysis using samples taken at a shooting range. The most suitable protocol for combined collection and analysis of IGSR and OGSR involved collection using GSR stubs followed by SEM-EDX analysis and liquid extraction using acetone followed by analysis with UHPLC.