RESUMEN
We have conducted a long-term research on the Taiwanese soft coral Asterospicularia laurae, which resulted in many xenicane-type diterpenoids such as asterolaurins A-M from A. laurae coral tissues during the non-spawning period were isolated. Here, we report a new xenicane diterpenoid, asterolaurin N (1), along with three known xenicane-type monocarbocyclic diterpenes [13-epi-9-desacetylxenicin (2), xeniolide-B 9-acetate (3) and asterolaurin I (4)] from A. laurae during the spawning period. The structures of the new secondary metabolite were established with an extensive spectroscopic analysis. The 1D and 2D nuclear magnetic resonance (NMR) data of the compounds were discussed. We discovered that the C-15 of 1 contains two methyl groups on a carbon bearing an acetyl group, which has not been reported previously. In addition, Compounds 1, 3, and 4 showed selective cytotoxic activity against Molt 4, while 2 exhibited significant cytotoxicity against Molt 4, K562, Sup-T1 and U937 cell lines.
Asunto(s)
Antozoos/metabolismo , Metabolismo Secundario , Animales , Antineoplásicos/química , Antineoplásicos/farmacología , Espectroscopía de Resonancia Magnética con Carbono-13 , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Diterpenos/química , Diterpenos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Concentración 50 Inhibidora , Espectroscopía de Protones por Resonancia Magnética , TaiwánRESUMEN
Excessive copper ions in drinking water could cause serious health issues, such as gastrointestinal disorders and cirrhosis, and they are associated with Alzheimer's disease. ICP-OES, ICP-MS, and AAS are the most common methods of copper ion determination. However, the high cost of sample preparation and labor limit the possibility of on-site detection. In this study, rapid monitoring of copper ion through the SERS technique was evaluated. Fe3O4@SiO2-Ag-4MBA nanoparticles were investigated as SERS-activated magnetic nanoprobes. These magnetic nanoprobes underwent superparamagnetism for rapid aggregation in seconds and provided selectivity in sensing copper ions. According to the dose-response curve of the SERS spectra, the limit of detection (LOD) was 0.421 ppm and the dynamic range was from 0.5 to 20 ppm in the presence of other metal ions. Copper ion detection through SERS was highly correlated with ICP-OES (R 2 = 0.95, slope = 0.974). These results demonstrate that magnetic nanoprobes may ultimately be used in a platform for on-site detection.